Current Protein Identity:P0A805
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1EK8 CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI Deposited 2000-03-07 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–185(185 aa)
|
Not recorded | HG MERCURY (II) ION × 3 DEM DECYLOXY-METHANOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.5;287 K;0.1 M MES-NaOH, 10 % PEG 350 MME, 12 % PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K
|
Resolution 2.30 Å R-free 0.297 |
| 1ISE Crystal structure of a mutant of ribosome recycling factor from Escherichia coli, Arg132Gly Deposited 2001-11-30 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–185(185 aa)
|
Mutation:R132G | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 5.8;277 K;50mM MES-NaOH, PEG 1500, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.20 Å R-free 0.297 |
| 1Y69 RRF domain I in complex with the 50S ribosomal subunit from Deinococcus radiodurans Deposited 2004-12-04 | Assembly 1 Protein–RNA Heteromer;Protein × 3 PDB declaration: pentameric(5) Consistent with all polymers |
Chain 8
1–30(30 aa)
Fragment:UNP residues 1-30 and 106-185
Chain 8
106–185(80 aa)
Fragment:UNP residues 1-30 and 106-185
|
Mutation:RRF domain II deletion and GGG insertion Mutation:RRF domain II deletion and GGG insertion | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.8;290 K;ETHANOL, DIMETHYLHEXANEDIOL, MGCL2, KCL, HEPES, NH4CL, pH 7.80, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 3.33 Å R-free 0.338 |
| 1ZN0 Coordinates of RRF and EF-G fitted into Cryo-EM map of the 50S subunit bound with both EF-G (GDPNP) and RRF Deposited 2005-05-11 | Assembly 1 Protein–RNA Heteromer;Protein × 2 PDB declaration: trimeric(3) Consistent with all polymers |
Chain A
1–185(185 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
Polymix buffer;pH 7.5;Polymix buffer
cryo-EM vitrification conditions
Cryogen ETHANE;Rapid-freezing in liquid ethane
|
Resolution 15.50 Å |
| 1ZN1 Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex Deposited 2005-05-11 | Assembly 1 Protein–RNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
1–185(185 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
Polymix buffer;pH 7.5;Polymix buffer
cryo-EM vitrification conditions
Cryogen ETHANE;Rapid-freezing in liquid ethane
|
Resolution 14.10 Å |
| 2RDO 50S subunit with EF-G(GDPNP) and RRF bound Deposited 2007-09-24 | Assembly 1 Protein–RNA Heteromer;Protein × 32 PDB declaration: 34-meric(34) Consistent with all polymers |
Chain 8
1–185(185 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
PolyMix;pH 7.5;PolyMix
cryo-EM vitrification conditions
Cryogen ETHANE;Rapid-freezing in liquid ethane by Vitrobot
|
Resolution 9.10 Å |
| 4V9C Allosteric control of the ribosome by small-molecule antibiotics Deposited 2012-07-25 | Assembly 2 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers |
Chain CY
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 266 NMY NEOMYCIN × 8 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5;291 K;PEG800, MPD, pH 6.5, microbatch, temperature 291K
|
Resolution 3.30 Å R-free 0.255 |
| 4V9D Structures of the bacterial ribosome in classical and hybrid states of tRNA binding Deposited 2012-07-31 | Assembly 1 Protein–RNA Heteromer;Protein × 50 PDB declaration: 55-meric(55) Consistent with all polymers |
Chain AY
2–183(182 aa)
|
Not recorded | MG MAGNESIUM ION × 271 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH;pH 6.5;291 K;PEG8000, MPD, pH 6.5, microbatch, temperature 291K
X-ray crystallization conditions
MICROBATCH;pH 6.5;291 K;PEG8000, MPD, pH 6.5, microbatch, temperature 291K
X-ray crystallization conditions
MICROBATCH;pH 6.5;291 K;PEG8000, MPD, pH 6.5, microbatch, temperature 291K
X-ray crystallization conditions
MICROBATCH;pH 6.5;291 K;PEG8000, MPD, pH 6.5, microbatch, temperature 291K
X-ray crystallization conditions
MICROBATCH;pH 6.5;291 K;PEG8000, MPD, pH 6.5, microbatch, temperature 291K
|
Resolution 3.00 Å R-free 0.260 |
| 4WOI 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 Deposited 2014-10-15 | Assembly 1 Protein–RNA Heteromer;Protein × 51 PDB declaration: 56-meric(56) Consistent with all polymers |
Chain AV
1–185(185 aa)
|
Not recorded | MG MAGNESIUM ION × 269 PAR PAROMOMYCIN × 6 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
MICROBATCH;pH 6.5;291 K;PEG8k, MPD, KCl, KSCN
|
Resolution 3.00 Å R-free 0.253 |