当前蛋白身份：P10587
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1BR1 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.ALF4 BOUND AT THE ACTIVE SITE 提交 1998-08-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
2–818（817 aa）
片段：CHAINS A, C, E, G, MOTOR DOMAIN, CHAINS B, D, F, H, ESSENTIAL LIGHT
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.2；277 K；1.8M AMMONIUM SULFATE 100MM HEPES PH 7.2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 3.50 Å R-free 0.305 |
| 1BR1 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.ALF4 BOUND AT THE ACTIVE SITE 提交 1998-08-26 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
2–818（817 aa）
片段：CHAINS A, C, E, G, MOTOR DOMAIN, CHAINS B, D, F, H, ESSENTIAL LIGHT
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.2；277 K；1.8M AMMONIUM SULFATE 100MM HEPES PH 7.2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 3.50 Å R-free 0.305 |
| 1BR1 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.ALF4 BOUND AT THE ACTIVE SITE 提交 1998-08-26 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
2–818（817 aa）
片段：CHAINS A, C, E, G, MOTOR DOMAIN, CHAINS B, D, F, H, ESSENTIAL LIGHT
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.2；277 K；1.8M AMMONIUM SULFATE 100MM HEPES PH 7.2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 3.50 Å R-free 0.305 |
| 1BR1 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.ALF4 BOUND AT THE ACTIVE SITE 提交 1998-08-26 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 G
2–818（817 aa）
片段：CHAINS A, C, E, G, MOTOR DOMAIN, CHAINS B, D, F, H, ESSENTIAL LIGHT
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.2；277 K；1.8M AMMONIUM SULFATE 100MM HEPES PH 7.2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 3.50 Å R-free 0.305 |
| 1BR2 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN COMPLEXED WITH MGADP.ALF4 提交 1998-08-26 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
2–791（790 aa）
片段：MOTOR DOMAIN
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.8；277 K；12% PEG8000 100MM IMIDAZOLE PH 6.8 150MM CACL2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 2.90 Å R-free 0.291 |
| 1BR2 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN COMPLEXED WITH MGADP.ALF4 提交 1998-08-26 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
2–791（790 aa）
片段：MOTOR DOMAIN
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.8；277 K；12% PEG8000 100MM IMIDAZOLE PH 6.8 150MM CACL2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 2.90 Å R-free 0.291 |
| 1BR2 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN COMPLEXED WITH MGADP.ALF4 提交 1998-08-26 | Assembly 3 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 C
2–791（790 aa）
片段：MOTOR DOMAIN
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.8；277 K；12% PEG8000 100MM IMIDAZOLE PH 6.8 150MM CACL2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 2.90 Å R-free 0.291 |
| 1BR2 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN COMPLEXED WITH MGADP.ALF4 提交 1998-08-26 | Assembly 4 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 D
2–791（790 aa）
片段：MOTOR DOMAIN
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.8；277 K；12% PEG8000 100MM IMIDAZOLE PH 6.8 150MM CACL2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 2.90 Å R-free 0.291 |
| 1BR2 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN COMPLEXED WITH MGADP.ALF4 提交 1998-08-26 | Assembly 5 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 E
2–791（790 aa）
片段：MOTOR DOMAIN
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.8；277 K；12% PEG8000 100MM IMIDAZOLE PH 6.8 150MM CACL2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 2.90 Å R-free 0.291 |
| 1BR2 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN COMPLEXED WITH MGADP.ALF4 提交 1998-08-26 | Assembly 6 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 F
2–791（790 aa）
片段：MOTOR DOMAIN
|
未记录 | MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.8；277 K；12% PEG8000 100MM IMIDAZOLE PH 6.8 150MM CACL2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 2.90 Å R-free 0.291 |
| 1BR4 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.BEF3 BOUND AT THE ACTIVE SITE 提交 1998-08-27 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
2–818（817 aa）
片段：CHAINS A, C, E, G, MOTOR DOMAIN, CHAINS B, D, F, H, ESSENTIAL LIGHT
|
未记录 | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.2；277 K；1.8M AMMONIUM SULFATE 100MM HEPES PH 7.2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 3.62 Å R-free 0.352 |
| 1BR4 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.BEF3 BOUND AT THE ACTIVE SITE 提交 1998-08-27 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
2–818（817 aa）
片段：CHAINS A, C, E, G, MOTOR DOMAIN, CHAINS B, D, F, H, ESSENTIAL LIGHT
|
未记录 | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.2；277 K；1.8M AMMONIUM SULFATE 100MM HEPES PH 7.2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 3.62 Å R-free 0.352 |
| 1BR4 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.BEF3 BOUND AT THE ACTIVE SITE 提交 1998-08-27 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
2–818（817 aa）
片段：CHAINS A, C, E, G, MOTOR DOMAIN, CHAINS B, D, F, H, ESSENTIAL LIGHT
|
未记录 | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.2；277 K；1.8M AMMONIUM SULFATE 100MM HEPES PH 7.2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 3.62 Å R-free 0.352 |
| 1BR4 SMOOTH MUSCLE MYOSIN MOTOR DOMAIN-ESSENTIAL LIGHT CHAIN COMPLEX WITH MGADP.BEF3 BOUND AT THE ACTIVE SITE 提交 1998-08-27 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 G
2–818（817 aa）
片段：CHAINS A, C, E, G, MOTOR DOMAIN, CHAINS B, D, F, H, ESSENTIAL LIGHT
|
未记录 | MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.2；277 K；1.8M AMMONIUM SULFATE 100MM HEPES PH 7.2 2MM MGADP 2MM AL(NO3)3 8MM NAF PROTEIN CONCENTRATION: 10MG/ML TEMPERATURE: 4 DEGREES CENTIGRADE, temperature 277K
|
分辨率 3.62 Å R-free 0.352 |
| 1I84 CRYO-EM STRUCTURE OF THE HEAVY MEROMYOSIN SUBFRAGMENT OF CHICKEN GIZZARD SMOOTH MUSCLE MYOSIN WITH REGULATORY LIGHT CHAIN IN THE DEPHOSPHORYLATED STATE. ONLY C ALPHAS PROVIDED FOR REGULATORY LIGHT CHAIN. ONLY BACKBONE ATOMS PROVIDED FOR S2 FRAGMENT. 提交 2001-03-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 S
1–1174（1174 aa）
片段：MEROMYOSIN SUBFRAGMENT. S1 AND S2 FRAGMENTS.
链 V
1–1174（1174 aa）
片段：MEROMYOSIN SUBFRAGMENT. S1 AND S2 FRAGMENTS.
|
非标准单体：是（mmCIF未提供具体位点） 非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | ELECTRON CRYSTALLOGRAPHY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；Specimens were frozen in liquid ethane using a drop
freezing apparatus.
X-ray结晶条件
Lipid monolayer；pH 7.5；277 K；MgCl, sodium phosphate, ATP, EGTA, PEG 6000, pH 7.5, Lipid monolayer, temperature 277K
|
分辨率 20.00 Å |
| 3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP 提交 2008-07-15 | Assembly 1 信息不足 异源复合物；蛋白 × 216 PDB 声明：216-meric（216） 与蛋白数一致 |
链 A
3–852（850 aa）
片段：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
链 B
3–852（850 aa）
片段：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM玻璃化条件
Plunging in a liquid ethane.
Blotting was performed from one side of the grid till a thin sample film on it using Whatman
No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled
by liquid nitrogen.
Grids were stored under liquid nitrogen.
|
分辨率 20.00 Å |
| 3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP 提交 2008-07-15 | Assembly 2 信息不足 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
3–852（850 aa）
片段：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
链 B
3–852（850 aa）
片段：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM玻璃化条件
Plunging in a liquid ethane.
Blotting was performed from one side of the grid till a thin sample film on it using Whatman
No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled
by liquid nitrogen.
Grids were stored under liquid nitrogen.
|
分辨率 20.00 Å |
| 3DTP Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP 提交 2008-07-15 | Assembly 3 信息不足 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
3–852（850 aa）
片段：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-972)
链 B
3–852（850 aa）
片段：SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974),SUBFRAGMENT 1(S1), DELTA-S2 (residues 2-974)
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3；pH 7；100mM NaCl, 3mM MgCl2, 1mM EGTA, 5mM PIPES, 5mM NaH2PO4, 1mM NaN3
cryo-EM玻璃化条件
Plunging in a liquid ethane.
Blotting was performed from one side of the grid till a thin sample film on it using Whatman
No 42 filter paper, then the grid was immediately plunged under gravity into liquid ethane cooled
by liquid nitrogen.
Grids were stored under liquid nitrogen.
|
分辨率 20.00 Å |
| 3J04 EM structure of the heavy meromyosin subfragment of Chick smooth muscle Myosin with regulatory light chain in phosphorylated state 提交 2011-02-18 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
2–910（909 aa）
片段：MEROMYOSIN SUBFRAGMENT
链 D
2–910（909 aa）
片段：MEROMYOSIN SUBFRAGMENT
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.8；1 mM Mg, 20 mM phosphate, 1 mM ATP, 1 mM EGTA, 7-10% polyethylene glycol 6000, 90-120 mM NaCl
cryo-EM玻璃化条件
Blot for 4 sec before plunging；冷冻剂 ETHANE；Carried out in cold room at 4 degrees C
X-ray结晶条件
pH 7.8；pH 7.8
|
分辨率 20.00 Å |
| 5M05 Chicken smooth muscle myosin motor domain co-crystallized with the specific CK-571 inhibitor, MgADP form 提交 2016-10-03 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–790（790 aa）
|
未记录 | 52E 4-{[(2-chloro-3-fluorobenzyl)carbamoyl](methyl)amino}-3,4-dideoxy-5-O-(isoquinolin-3-ylcarbamoyl)-D-erythro-pentitol × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.2；277 K；Peg8K, 50 mM Bicine pH 8.2, 10% DMSO
|
分辨率 2.67 Å R-free 0.244 |
| 6BIH The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State 提交 2017-11-02 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 14 PDB 声明：tetradecameric（14） 与蛋白数一致 |
链 H
1–790（790 aa）
|
未记录 | ADP ADENOSINE-5'-DIPHOSPHATE × 7 MG MAGNESIUM ION × 7 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；actin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.4, myosin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.0
cryo-EM玻璃化条件
冷冻剂 ETHANE；Some specimens were frozen manually using a homemade plunger.
|
分辨率 6.00 Å |
| 6BIH The Structure of the Actin-Smooth Muscle Myosin Motor Domain Complex in the Rigor State 提交 2017-11-02 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 H
1–790（790 aa）
|
未记录 | ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；actin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.4, myosin buffer: 10 mM imidazole, 10 mM KCl, 1.0 mM MgCl2, 1.0 mM EGTA, 0.5 mM DTT, pH 7.0
cryo-EM玻璃化条件
冷冻剂 ETHANE；Some specimens were frozen manually using a homemade plunger.
|
分辨率 6.00 Å |
| 7MF3 Structure of the autoinhibited state of smooth muscle myosin-2 提交 2021-04-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 A
2–1979（1978 aa）
链 B
2–1979（1978 aa）
链 G
2–1979（1978 aa）
链 H
2–1979（1978 aa）
|
未记录 | ADP ADENOSINE-5'-DIPHOSPHATE × 2 PO4 PHOSPHATE ION × 2 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.3；150 mM NaCl, 1mM EGTA, 2mM MgCl2
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.40 Å |