Current Protein Identity：P10822
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1EYL STRUCTURE OF A RECOMBINANT WINGED BEAN CHYMOTRYPSIN INHIBITOR Deposited 2000-05-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Not recorded | SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.4；277 K；ammonium sulfate, sodium acetate, pH 5.4, VAPOR DIFFUSION, temperature 277.0K
|
Resolution 1.90 Å R-free 0.246 |
| 1EYL STRUCTURE OF A RECOMBINANT WINGED BEAN CHYMOTRYPSIN INHIBITOR Deposited 2000-05-07 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Not recorded | SO4 SULFATE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.4；277 K；ammonium sulfate, sodium acetate, pH 5.4, VAPOR DIFFUSION, temperature 277.0K
|
Resolution 1.90 Å R-free 0.246 |
| 1EYL STRUCTURE OF A RECOMBINANT WINGED BEAN CHYMOTRYPSIN INHIBITOR Deposited 2000-05-07 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Not recorded | SO4 SULFATE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.4；277 K；ammonium sulfate, sodium acetate, pH 5.4, VAPOR DIFFUSION, temperature 277.0K
|
Resolution 1.90 Å R-free 0.246 |
| 1FMZ CRYSTAL STRUCTURE OF A MUTANT WINGED BEAN CHYMOTRYPSIN INHIBITOR PROTEIN, N14K. Deposited 2000-08-19 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N14K | SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；Ammonium sulfate, Sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
Resolution 2.05 Å R-free 0.253 |
| 1FMZ CRYSTAL STRUCTURE OF A MUTANT WINGED BEAN CHYMOTRYPSIN INHIBITOR PROTEIN, N14K. Deposited 2000-08-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N14K | SO4 SULFATE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；Ammonium sulfate, Sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
Resolution 2.05 Å R-free 0.253 |
| 1FMZ CRYSTAL STRUCTURE OF A MUTANT WINGED BEAN CHYMOTRYPSIN INHIBITOR PROTEIN, N14K. Deposited 2000-08-19 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N14K | SO4 SULFATE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；Ammonium sulfate, Sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
Resolution 2.05 Å R-free 0.253 |
| 1FN0 STRUCTURE OF A MUTANT WINGED BEAN CHYMOTRYPSIN INHIBITOR PROTEIN, N14D. Deposited 2000-08-19 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N14D | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；Ammonium sulfate, Sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
Resolution 2.00 Å R-free 0.287 |
| 1FN0 STRUCTURE OF A MUTANT WINGED BEAN CHYMOTRYPSIN INHIBITOR PROTEIN, N14D. Deposited 2000-08-19 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N14D | SO4 SULFATE ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；Ammonium sulfate, Sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
Resolution 2.00 Å R-free 0.287 |
| 1FN0 STRUCTURE OF A MUTANT WINGED BEAN CHYMOTRYPSIN INHIBITOR PROTEIN, N14D. Deposited 2000-08-19 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N14D | SO4 SULFATE ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；Ammonium sulfate, Sodium acetate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
Resolution 2.00 Å R-free 0.287 |
| 1WBC CRYSTALLIZATION AND PRELIMINARY X-RAY STUDIES OF PSOPHOCARPIN B1, A CHYMOTRYPSIN INHIBITOR FROM WINGED BEAN SEEDS Deposited 1995-11-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.95 Å |
| 1XG6 The crystal structure of the P1 mutant (Leu to Arg)of a Winged bean chymotrypsin inhibitor(Kunitz)solved at 2.15A resolution Deposited 2004-09-16 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：L68R | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.15 Å R-free 0.236 |
| 2BEA Crystal structure of Asn14 to Gly mutant of WCI Deposited 2005-10-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N14G | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.35 Å R-free 0.263 |
| 2BEA Crystal structure of Asn14 to Gly mutant of WCI Deposited 2005-10-24 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
25–207（183 aa）
|
Mutation：N14G | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.35 Å R-free 0.263 |
| 2BEB X-ray structure of Asn to Thr mutant of Winged Bean Chymotrypsin inhibitor Deposited 2005-10-24 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N17T | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；5% PEG 4000, 50 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.81 Å R-free 0.254 |
| 2BEB X-ray structure of Asn to Thr mutant of Winged Bean Chymotrypsin inhibitor Deposited 2005-10-24 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
25–207（183 aa）
|
Mutation：N17T | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；5% PEG 4000, 50 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.81 Å R-free 0.254 |
| 2ESU Crystal structure of Asn to Gln mutant of Winged Bean Chymotrypsin Inhibitor protein Deposited 2005-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N17Q | NI NICKEL (II) ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；25.4% PEG 8000, 0.17M Ammonium Sulphate, 15% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 1.94 Å R-free 0.238 |
| 2ESU Crystal structure of Asn to Gln mutant of Winged Bean Chymotrypsin Inhibitor protein Deposited 2005-10-27 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N17Q | NI NICKEL (II) ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；25.4% PEG 8000, 0.17M Ammonium Sulphate, 15% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 1.94 Å R-free 0.238 |
| 2ET2 Crystal structure of an Asn to Ala mutant of Winged Bean Chymotrypsin Inhibitor protein Deposited 2005-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N17A | NI NICKEL (II) ION × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；25.5% PEG 8K, 0.17 M Ammonium Sulphate, 15% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.10 Å R-free 0.275 |
| 2ET2 Crystal structure of an Asn to Ala mutant of Winged Bean Chymotrypsin Inhibitor protein Deposited 2005-10-27 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：N17A | NI NICKEL (II) ION × 2 SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；25.5% PEG 8K, 0.17 M Ammonium Sulphate, 15% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.10 Å R-free 0.275 |
| 2QYI Crystal structure of a binary complex between an engineered trypsin inhibitor and Bovine trypsin Deposited 2007-08-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
25–207（183 aa）
Chain D
25–207（183 aa）
|
Mutation：L68R Mutation：L68R | CA CALCIUM ION × 2 NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；30% Jefamine, VAPOR DIFFUSION, temperature 293K
|
Resolution 2.60 Å R-free 0.268 |
| 2WBC REFINED CRYSTAL STRUCTURE (2.3 ANGSTROM) OF A WINGED BEAN CHYMOTRYPSIN INHIBITOR AND LOCATION OF ITS SECOND REACTIVE SITE Deposited 1997-11-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；277 K；PROTEIN WAS CRYSTALLIZED BY HANGING DROP VAPOUR DIFFUSION FROM 25% AMM. SULPHATE IN TRIS-HCL, 10MM NA ACETATE,400 MM NACL AT PH 5.4 AGAINST 25% AMM. SULPHATE,60MM NA ACETATE AT 4 DEGREE CENTIGRADE., vapor diffusion - hanging drop, temperature 277K
|
Resolution 2.30 Å R-free 0.241 |
| 3I29 Crystal structure of a binary complex between an mutant trypsin inhibitor with bovine trypsin Deposited 2009-06-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
25–207（183 aa）
|
Mutation：F64Y, L65R | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.40 Å R-free 0.287 |
| 3I2A Crystal structure of a chimeric trypsin inhibitor protein STI(L)-WCI(S) Deposited 2009-06-29 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
Chain B
25–207（183 aa）
|
Mutation：Q66P, F67Y, L68R, S69I, L70R,Q66P, F67Y, L68R, S69I, L70R, Q266P, F267Y, L268R, S269I, L270R Mutation：Q66P, F67Y, L68R, S69I, L70R,Q66P, F67Y, L68R, S69I, L70R, Q266P, F267Y, L268R, S269I, L270R | CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；PEG6000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.289 |
| 3I2X Crystal structure of a chimeric trypsin inhibitor having reactive site loop of ETI on the scaffold of WCI Deposited 2009-06-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：Q66P, F67L, L68R, L70A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.85 Å R-free 0.285 |
| 3I2X Crystal structure of a chimeric trypsin inhibitor having reactive site loop of ETI on the scaffold of WCI Deposited 2009-06-30 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
25–207（183 aa）
|
Mutation：Q66P, F67L, L68R, L70A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.85 Å R-free 0.285 |
| 3QYD Crystal structure of a recombinant chimeric trypsin inhibitor Deposited 2011-03-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–201（177 aa）
|
Mutation：Q66R, F67L, L68R, L70A, A79R, L118Y | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；35% PEG, 0.1M Tris, 5% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.97 Å R-free 0.270 |
| 3QYD Crystal structure of a recombinant chimeric trypsin inhibitor Deposited 2011-03-03 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
25–201（177 aa）
|
Mutation：Q66R, F67L, L68R, L70A, A79R, L118Y | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；35% PEG, 0.1M Tris, 5% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.97 Å R-free 0.270 |
| 3QYD Crystal structure of a recombinant chimeric trypsin inhibitor Deposited 2011-03-03 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
25–201（177 aa）
|
Mutation：Q66R, F67L, L68R, L70A, A79R, L118Y | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；35% PEG, 0.1M Tris, 5% glycerol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.97 Å R-free 0.270 |
| 3VEQ A binary complex betwwen bovine pancreatic trypsin and a engineered mutant trypsin inhibitor Deposited 2012-01-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：L668A | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.25 Å R-free 0.274 |
| 4H9W crystal structure of a METHIONINE mutant of WCI Deposited 2012-09-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Mutation：W91M | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；0.3M AMS, 5% GLYCEROL, 25% PEG 6K, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.50 Å R-free 0.257 |
| 4HA2 Crystal structure ofa phenyl alanine 91 mutant of WCI Deposited 2012-09-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Not recorded | NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；5%PEG 6K, 10%GLYCEROL, 0.1M CITRIC ACID, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.90 Å R-free 0.282 |
| 4HA2 Crystal structure ofa phenyl alanine 91 mutant of WCI Deposited 2012-09-25 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
25–207（183 aa）
|
Not recorded | NI NICKEL (II) ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；5%PEG 6K, 10%GLYCEROL, 0.1M CITRIC ACID, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.90 Å R-free 0.282 |
| 4TLP Crystal structure of a alanine91 mutant of WCI Deposited 2014-05-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
26–200（175 aa）
Fragment：UNP residues 26-200
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；30% PEG6000, 0.1M Sodium Citrate
|
Resolution 1.90 Å R-free 0.205 |
| 4WBC 2.13 A STRUCTURE OF A KUNITZ-TYPE WINGED BEAN CHYMOTRYPSIN INHIBITOR PROTEIN Deposited 1999-03-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
25–207（183 aa）
|
Not recorded | SO4 SULFATE ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；PROTEIN WAS CRYSTALLIZED BY HANGING DROP VAPOUR
DIFFUSION FROM 25% AMM. SULPHATE IN TRIS-HCL, 10MM NA ACETATE, 400 MM NACL AT
PH 5.4 AGAINST 25% AMM. SULPHATE,60MM NA ACETATE AT 4 DEG. C. CRYSTALS WERE
THEN TRANSFERED TO 25% GLYCEROL (CRYOPROTECTANT) IN 25% AMM. SULPHATE, 10MM NA
ACETATE BUFFER AT PH 5.4, BEFORE FLASH COOLING.
|
Resolution 2.14 Å R-free 0.257 |
| 4WBC 2.13 A STRUCTURE OF A KUNITZ-TYPE WINGED BEAN CHYMOTRYPSIN INHIBITOR PROTEIN Deposited 1999-03-04 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Not recorded | SO4 SULFATE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；PROTEIN WAS CRYSTALLIZED BY HANGING DROP VAPOUR
DIFFUSION FROM 25% AMM. SULPHATE IN TRIS-HCL, 10MM NA ACETATE, 400 MM NACL AT
PH 5.4 AGAINST 25% AMM. SULPHATE,60MM NA ACETATE AT 4 DEG. C. CRYSTALS WERE
THEN TRANSFERED TO 25% GLYCEROL (CRYOPROTECTANT) IN 25% AMM. SULPHATE, 10MM NA
ACETATE BUFFER AT PH 5.4, BEFORE FLASH COOLING.
|
Resolution 2.14 Å R-free 0.257 |
| 4WBC 2.13 A STRUCTURE OF A KUNITZ-TYPE WINGED BEAN CHYMOTRYPSIN INHIBITOR PROTEIN Deposited 1999-03-04 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
25–207（183 aa）
|
Not recorded | SO4 SULFATE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.4；PROTEIN WAS CRYSTALLIZED BY HANGING DROP VAPOUR
DIFFUSION FROM 25% AMM. SULPHATE IN TRIS-HCL, 10MM NA ACETATE, 400 MM NACL AT
PH 5.4 AGAINST 25% AMM. SULPHATE,60MM NA ACETATE AT 4 DEG. C. CRYSTALS WERE
THEN TRANSFERED TO 25% GLYCEROL (CRYOPROTECTANT) IN 25% AMM. SULPHATE, 10MM NA
ACETATE BUFFER AT PH 5.4, BEFORE FLASH COOLING.
|
Resolution 2.14 Å R-free 0.257 |