Current Protein Identity:P10958
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1D5W PHOSPHORYLATED FIXJ RECEIVER DOMAIN Deposited 1999-10-12 | Assembly 1 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
1–126(126 aa)
Fragment:FIXJ RECEIVER DOMAIN (RESIDUES 1-126)
|
Mutation:T2Q, A125L Non-standard monomer:Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.5;277 K;AMMONIUM SULFATE, 2-METHYL-2,4-PENTANEDIOL, HEPES, TRITON X-100, EDTA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.244 |
| 1D5W PHOSPHORYLATED FIXJ RECEIVER DOMAIN Deposited 1999-10-12 | Assembly 2 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
1–126(126 aa)
Fragment:FIXJ RECEIVER DOMAIN (RESIDUES 1-126)
Chain C
1–126(126 aa)
Fragment:FIXJ RECEIVER DOMAIN (RESIDUES 1-126)
|
Mutation:T2Q, A125L Non-standard monomer:Yes (specific site not provided by mmCIF) Mutation:T2Q, A125L Non-standard monomer:Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.5;277 K;AMMONIUM SULFATE, 2-METHYL-2,4-PENTANEDIOL, HEPES, TRITON X-100, EDTA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.30 Å R-free 0.244 |
| 1DBW CRYSTAL STRUCTURE OF FIXJ-N Deposited 1999-11-03 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–126(126 aa)
Fragment:FIXJ RECEIVER DOMAIN (RESIDUES 1-126)
|
Mutation:T2Q, A125L | 15P POLYETHYLENE GLYCOL (N=34) × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;277 K;PEG 1500, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 4K
|
Resolution 1.60 Å R-free 0.224 |
| 1DBW CRYSTAL STRUCTURE OF FIXJ-N Deposited 1999-11-03 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
1–126(126 aa)
Fragment:FIXJ RECEIVER DOMAIN (RESIDUES 1-126)
|
Mutation:T2Q, A125L | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;277 K;PEG 1500, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 4K
|
Resolution 1.60 Å R-free 0.224 |
| 1DCK STRUCTURE OF UNPHOSPHORYLATED FIXJ-N COMPLEXED WITH MN2+ Deposited 1999-11-05 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–126(126 aa)
Fragment:N-TERMINAL DOMAIN (RESIDUES 1-126)
|
Mutation:T2Q, A125L | MN MANGANESE (II) ION × 1 15P POLYETHYLENE GLYCOL (N=34) × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;277 K;PEG1500, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 4K
|
Resolution 2.00 Å R-free 0.253 |
| 1DCK STRUCTURE OF UNPHOSPHORYLATED FIXJ-N COMPLEXED WITH MN2+ Deposited 1999-11-05 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
1–126(126 aa)
Fragment:N-TERMINAL DOMAIN (RESIDUES 1-126)
|
Mutation:T2Q, A125L | MN MANGANESE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;277 K;PEG1500, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 4K
|
Resolution 2.00 Å R-free 0.253 |
| 1DCM STRUCTURE OF UNPHOSPHORYLATED FIXJ-N WITH AN ATYPICAL CONFORMER (MONOMER A) Deposited 1999-11-05 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–126(126 aa)
Fragment:FIXJ RECEIVER DOMAIN (RESIDUES 1-126)
|
Mutation:T2Q, A125L | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.8;277 K;PEG1500, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 4K
|
Resolution 3.00 Å R-free 0.335 |
| 1DCM STRUCTURE OF UNPHOSPHORYLATED FIXJ-N WITH AN ATYPICAL CONFORMER (MONOMER A) Deposited 1999-11-05 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
1–126(126 aa)
Fragment:FIXJ RECEIVER DOMAIN (RESIDUES 1-126)
|
Mutation:T2Q, A125L | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7.8;277 K;PEG1500, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 4K
|
Resolution 3.00 Å R-free 0.335 |
| 1X3U Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium melilot Deposited 2005-05-10 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
130–204(75 aa)
Fragment:C-TERMINAL DOMAIN
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5.5;298 K;Pressure 1
NMR sample composition
1mM FixJC U-15N,13C; 20mM NaHPO4-NaH2PO4 (pH 5.5), 100mM NaCl, 50mM Na2SO4; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |