Current Protein Identity:P12071
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1H9H COMPLEX OF EETI-II WITH PORCINE TRYPSIN Deposited 2001-03-12 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain I
1–30(30 aa)
|
Mutation:YES | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.7;pH 6.70
|
Resolution 1.50 Å R-free 0.276 |
| 1H9I COMPLEX OF EETI-II MUTANT WITH PORCINE TRYPSIN Deposited 2001-03-12 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain I
1–30(30 aa)
|
Mutation:YES | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.7;pH 6.70
|
Resolution 1.90 Å R-free 0.175 |
| 1W7Z Crystal structure of the free (uncomplexed) Ecballium elaterium trypsin inhibitor (EETI-II) Deposited 2004-09-14 | Assembly 1 Protein homooligomer Homooligomer;Protein × 6 PDB declaration: hexameric(6) Consistent with protein count |
Chain A
1–31(31 aa)
Chain B
1–31(31 aa)
Chain C
1–31(31 aa)
Chain D
1–31(31 aa)
Chain E
1–31(31 aa)
Chain F
1–31(31 aa)
|
Not recorded | NA SODIUM ION × 3 FMT FORMIC ACID × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.6;2M NA-FORMATE 0.1 M NA-ACETATE PH 4.6
|
Resolution 1.67 Å R-free 0.235 |
| 1W7Z Crystal structure of the free (uncomplexed) Ecballium elaterium trypsin inhibitor (EETI-II) Deposited 2004-09-14 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain G
1–31(31 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.6;2M NA-FORMATE 0.1 M NA-ACETATE PH 4.6
|
Resolution 1.67 Å R-free 0.235 |
| 1W7Z Crystal structure of the free (uncomplexed) Ecballium elaterium trypsin inhibitor (EETI-II) Deposited 2004-09-14 | Assembly 3 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain H
1–31(31 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.6;2M NA-FORMATE 0.1 M NA-ACETATE PH 4.6
|
Resolution 1.67 Å R-free 0.235 |
| 2C4B Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A Deposited 2005-10-18 | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
16–29(14 aa)
|
Mutation:YES | 2PE NONAETHYLENE GLYCOL × 2 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 4 FMT FORMIC ACID × 2 SO4 SULFATE ION × 7 UNX UNKNOWN LIGAND × 15 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;277 K;1.3 M AMMONIUM SULPHATE, 7% PEG400 (V/V), 0.1 M MES PH 6.5 AS RESERVOIR SOLUTION. DROPLETS MIXED FROM 8 UL PROTEIN (30 MG/ML) AND 4 UL RESERVOIR. SITTING DROP VAPOR DIFFUSION. 4 DEG. C.
|
Resolution 1.30 Å R-free 0.164 |
| 2C4B Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A Deposited 2005-10-18 | Assembly 2 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
16–29(14 aa)
|
Mutation:YES | 2PE NONAETHYLENE GLYCOL × 2 EDO 1,2-ETHANEDIOL × 6 FMT FORMIC ACID × 1 SO4 SULFATE ION × 10 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;277 K;1.3 M AMMONIUM SULPHATE, 7% PEG400 (V/V), 0.1 M MES PH 6.5 AS RESERVOIR SOLUTION. DROPLETS MIXED FROM 8 UL PROTEIN (30 MG/ML) AND 4 UL RESERVOIR. SITTING DROP VAPOR DIFFUSION. 4 DEG. C.
|
Resolution 1.30 Å R-free 0.164 |
| 2ETI USE OF RESTRAINED MOLECULAR DYNAMICS IN WATER TO DETERMINE THREE-DIMENSIONAL PROTEIN STRUCTURE: PREDICTION OF THE THREE-DIMENSIONAL STRUCTURE OF ECBALLIUM ELATERIUM TRYPSIN INHIBITOR II Deposited 1991-07-15 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–28(28 aa)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |
| 2IT7 Solution structure of the squash trypsin inhibitor EETI-II Deposited 2006-10-19 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–28(28 aa)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 2.7;285 K;Pressure 1
NMR measurement conditions
pH 2.7;300 K;Pressure 1
NMR sample composition
4mM EETI-II, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
4mM EETI-II, D2O | D2O
|
Resolution not provided |
| 2LET AN 1H NMR DETERMINATION OF THE THREE DIMENSIONAL STRUCTURES OF MIRROR IMAGE FORMS OF A LEU-5 VARIANT OF THE TRYPSIN INHIBITOR ECBALLIUM ELATERIUM (EETI-II) Deposited 1994-01-04 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–28(28 aa)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR | mmCIF provides none of the parsed conditions | Resolution not provided |