Current Protein Identity:P12464
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2KRC Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase Deposited 2009-12-16 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
2–92(91 aa)
Fragment:residues 2-92
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.6;301 K;Ionic strength (raw mmCIF value) 10;Pressure 1
NMR sample composition
10 mM sodium chloride-1, 50 uM sodium azide-2, 20 mM phosphate buffer-3, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
10 mM sodium chloride-4, 50 uM sodium azide-5, 20 mM phosphate buffer-6, 14.29 mg/mL filamentous Pf1 bacteriophage-7, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
10 mM sodium chloride-8, 50 uM sodium azide-9, 20 mM phosphate buffer-10, 5 % polyacrylamide gel-11, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2M4K Solution structure of the delta subunit of RNA polymerase from Bacillus subtilis Deposited 2013-02-07 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
2–173(172 aa)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.6;300 K;Ionic strength (raw mmCIF value) 10;Pressure ambient
NMR sample composition
10 % [U-100% 2H] D2O, 10 mM sodium chloride, 50 uM sodium azide, 20 mM sodium phosphate, 90 % H2O, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 4NC7 N-terminal domain of delta-subunit of RNA polymerase complexed with I3C and nickel ions Deposited 2013-10-24 | Assembly 1 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
2–92(91 aa)
Fragment:UNP residues 2-92
Chain B
2–92(91 aa)
Fragment:UNP residues 2-92
|
Not recorded | I3C 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid × 1 NI NICKEL (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;290.15 K;2M sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
X-ray crystallization conditions
VAPOR DIFFUSION;pH 8.2;290.15 K;1.8M sodium/potassium phosphate, pH 8.2, VAPOR DIFFUSION, temperature 290.15K
|
Resolution 2.00 Å R-free 0.259 |
| 4NC7 N-terminal domain of delta-subunit of RNA polymerase complexed with I3C and nickel ions Deposited 2013-10-24 | Assembly 2 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
2–92(91 aa)
Fragment:UNP residues 2-92
|
Not recorded | I3C 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid × 2 NI NICKEL (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;290.15 K;2M sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
X-ray crystallization conditions
VAPOR DIFFUSION;pH 8.2;290.15 K;1.8M sodium/potassium phosphate, pH 8.2, VAPOR DIFFUSION, temperature 290.15K
|
Resolution 2.00 Å R-free 0.259 |
| 4NC7 N-terminal domain of delta-subunit of RNA polymerase complexed with I3C and nickel ions Deposited 2013-10-24 | Assembly 3 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
2–92(91 aa)
Fragment:UNP residues 2-92
|
Not recorded | NI NICKEL (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;290.15 K;2M sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
X-ray crystallization conditions
VAPOR DIFFUSION;pH 8.2;290.15 K;1.8M sodium/potassium phosphate, pH 8.2, VAPOR DIFFUSION, temperature 290.15K
|
Resolution 2.00 Å R-free 0.259 |
| 4NC7 N-terminal domain of delta-subunit of RNA polymerase complexed with I3C and nickel ions Deposited 2013-10-24 | Assembly 4 Protein homooligomer Homooligomer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain A
2–92(91 aa)
Fragment:UNP residues 2-92
Chain B
2–92(91 aa)
Fragment:UNP residues 2-92
|
Not recorded | I3C 5-amino-2,4,6-triiodobenzene-1,3-dicarboxylic acid × 2 NI NICKEL (II) ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;290.15 K;2M sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
X-ray crystallization conditions
VAPOR DIFFUSION;pH 8.2;290.15 K;1.8M sodium/potassium phosphate, pH 8.2, VAPOR DIFFUSION, temperature 290.15K
|
Resolution 2.00 Å R-free 0.259 |
| 4NC8 N-terminal domain of delta-subunit of RNA polymerase complexed with nickel ions Deposited 2013-10-24 | Assembly 1 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
2–92(91 aa)
Fragment:UNP residues 2-92
|
Not recorded | NI NICKEL (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;290.15 K;2M sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8.2;290.15 K;1.8M sodium/potassium phosphate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
|
Resolution 2.17 Å R-free 0.283 |
| 4NC8 N-terminal domain of delta-subunit of RNA polymerase complexed with nickel ions Deposited 2013-10-24 | Assembly 2 Protein homooligomer Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
2–92(91 aa)
Fragment:UNP residues 2-92
|
Not recorded | NI NICKEL (II) ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;290.15 K;2M sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8.2;290.15 K;1.8M sodium/potassium phosphate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
|
Resolution 2.17 Å R-free 0.283 |
| 4NC8 N-terminal domain of delta-subunit of RNA polymerase complexed with nickel ions Deposited 2013-10-24 | Assembly 3 Protein homooligomer Homooligomer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain A
2–92(91 aa)
Fragment:UNP residues 2-92
Chain B
2–92(91 aa)
Fragment:UNP residues 2-92
|
Not recorded | NI NICKEL (II) ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;290.15 K;2M sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8.2;290.15 K;1.8M sodium/potassium phosphate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
|
Resolution 2.17 Å R-free 0.283 |
| 7F75 Cryo-EM structure of Spx-dependent transcription activation complex Deposited 2021-06-28 | Assembly 1 Protein–DNA Heteromer;Protein × 10 PDB declaration: dodecameric(12) Consistent with all polymers |
Chain L
1–173(173 aa)
|
Not recorded | MG MAGNESIUM ION × 1 ZN ZINC ION × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å |
| 8XA6 Cryo-EM structure of Bacillus RNAP and SPO1 gp33 complex Deposited 2023-12-02 | Assembly 1 Protein heterocomplex Heteromer;Protein × 8 PDB declaration: octameric(8) Consistent with protein count |
Chain L
1–173(173 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.02 Å |
| 8XA7 Cryo-EM structure of Bacillus subtilis RNAP,sigA and SPO1 gp33 complex Deposited 2023-12-02 | Assembly 1 Protein heterocomplex Heteromer;Protein × 9 PDB declaration: nonameric(9) Consistent with protein count |
Chain L
1–173(173 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.94 Å |
| 8XA8 Cryo-EM structure of Bacillus RNAP and HelD complex Deposited 2023-12-02 | Assembly 1 Protein heterocomplex Heteromer;Protein × 8 PDB declaration: octameric(8) Consistent with protein count |
Chain I
1–173(173 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.19 Å |