Current Protein Identity：P12676
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1B4V CHOLESTEROL OXIDASE FROM STREPTOMYCES Deposited 1998-12-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；PRECIPITANT CONDITIONS: 10-
12% PEG 8000, 100MM SODIUM CACODYLATE PH 5.2, 75MM MNSO4 PROTEIN CONCENTRATION
8.5MG/ML, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.50 Å R-free 0.200 |
| 1B8S CHOLESTEROL OXIDASE FROM STREPTOMYCES GLU361GLN MUTANT Deposited 1999-02-02 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
46–536（491 aa）
|
Mutation：GLU361GLN | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；PRECIPITANT CONDITIONS: 10-12% PEG 8000,
100MM SODIUM CACODYLATE PH 5.2,
75MM MNSO4
PROTEIN CONCENTRATION 8.5MG/ML
, VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.65 Å R-free 0.231 |
| 1CBO CHOLESTEROL OXIDASE FROM STREPTOMYCES HIS447ASN MUTANT Deposited 1999-02-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Mutation：H447N | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.2；CRYSTALLIZATION CONDITIONS: VAPOUR DIFFUSION USING THE HANGING DROP TECHNIQUE,
PRECIPITANT CONDITIONS: 10-12% PEG 8000, 100MM SODIUM CACODYLATE PH 5.2, 75MM
MNSO4 PROTEIN CONCENTRATION 8.5MG/ML
|
Resolution 1.80 Å R-free 0.194 |
| 1CC2 CHOLESTEROL OXIDASE FROM STREPTOMYCES HIS447GLN MUTANT Deposited 1999-03-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Mutation：H447Q | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.2；CRYSTALLIZATION CONDITIONS: VAPOUR DIFFUSION USING THE HANGING DROP TECHNIQUE,
PRECIPITANT CONDITIONS: 10-12% PEG 8000 100MM SODIUM CACODYLATE PH 5.2, 75MM
MNSO4 PROTEIN CONCENTRATION 8.5MG/ML
|
Resolution 2.20 Å R-free 0.235 |
| 1IJH CHOLESTEROL OXIDASE FROM STREPTOMYCES ASN485LEU MUTANT Deposited 2001-04-26 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Mutation：N485L | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；290 K；PEG 8000, SODIUM CACODYLATE, MNSO4, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 1.53 Å R-free 0.213 |
| 1MXT Atomic resolution structure of Cholesterol oxidase (Streptomyces sp. SA-COO) Deposited 2002-10-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | SO4 SULFATE ION × 1 FAE FLAVIN-N7 PROTONATED-ADENINE DINUCLEOTIDE × 1 OXY OXYGEN MOLECULE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；290 K；PEG 8000, manganese sulfate, cacodylate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 0.95 Å R-free 0.132 |
| 1N1P ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ pH 7.4 (STREPTOMYCES SP. SA-COO) Deposited 2002-10-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | MN MANGANESE (II) ION × 3 FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；290 K；PEG 8000, MANGANESE SULFATE,GLYCINE, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 0.95 Å R-free 0.119 |
| 1N4U CHOLESTEROL OXIDASE FROM STREPTOMYCES @ pH 4.5 (STREPTOMYCES SP. SA-COO) Deposited 2002-11-01 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | PO4 PHOSPHATE ION × 5 FAE FLAVIN-N7 PROTONATED-ADENINE DINUCLEOTIDE × 1 OXY OXYGEN MOLECULE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.5；pH 4.5
|
Resolution 0.95 Å R-free 0.136 |
| 1N4V ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @pH 5.8 (STREPTOMYCES SP. SA-COO) Deposited 2002-11-01 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.8；PEG 8000, MANGANESE SULFATE, CACODYLATE, pH 5.8
|
Resolution 1.00 Å R-free 0.147 |
| 1N4W ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ pH 7.3 (STREPTOMYCES SP. SA-COO) Deposited 2002-11-01 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | MN MANGANESE (II) ION × 1 FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.3；PEG 8000, MANGANESE SULFATE, MES, pH 7.3
|
Resolution 0.92 Å R-free 0.122 |
| 2GEW Atomic resolution structure of cholesterol oxidase @ pH 9.0 (Streptomyces sp. SA-COO) Deposited 2006-03-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | SO4 SULFATE ION × 1 FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 OXY OXYGEN MOLECULE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 9；298 K；PEG 8000, AMMONIUM SULFATE, PIPES PH 7.5,VAPOR DIFFUSION, HANGING DROP, temperature 298K,
SOAK CONDITION STEP 1: PEG 8000, AMMONIUM SULFATE, HEPES PH 8.0
SOAK CONDITION STEP 2: PEG 8000, AMMONIUM SULFATE, TRICINE PH 8.5
SOAK CONDITION STEP 3: PEG 8000, AMMONIUM SULFATE, CHES PH 9.0,
|
Resolution 0.97 Å R-free 0.140 |
| 3B3R Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A) Deposited 2007-10-22 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
42–546（505 aa）
|
Mutation：E361Q/H447Q | SO4 SULFATE ION × 2 FAE FLAVIN-N7 PROTONATED-ADENINE DINUCLEOTIDE × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；10% PEG 8000, 75mM magnesium sulfate, 100mM cacodylate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 0.98 Å R-free 0.159 |
| 3B6D Crystal Structure of Streptomyces Cholesterol Oxidase H447Q/E361Q mutant (1.2A) Deposited 2007-10-29 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Mutation：H447Q/E361Q | SO4 SULFATE ION × 1 FAE FLAVIN-N7 PROTONATED-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；10% PEG 8000, 75mM MnSO4, 100mM CACODYLATE pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.20 Å R-free 0.172 |
| 3CNJ Cholesterol oxidase from Streptomyces sp. F359W mutant (0.95A) Deposited 2008-03-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
45–543（499 aa）
|
Mutation：F359W | SO4 SULFATE ION × 2 FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；PEG 8000, MANGANESE SULFATE, CACODYLATE (PH 5.2), VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 0.95 Å R-free 0.175 |
| 3GYI Cholesterol oxidase from Streptomyces sp. N485D mutant (1.0A) Deposited 2009-04-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Mutation：N485D | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；9-11% PEG 8000, 75 mM MnSO4, 100 mM cacodylate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 1.00 Å R-free 0.158 |
| 3GYJ Cholesterol oxidase from Streptomyces sp. N485L mutant (0.92A) Deposited 2009-04-03 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Mutation：N485L | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；9-11% PEG 8000, 75 mM MnSO4, 100 mM cacodylate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 0.92 Å R-free 0.151 |
| 4REK Crystal structure and charge density studies of cholesterol oxidase from Brevibacterium sterolicum at 0.74 ultra-high resolution Deposited 2014-09-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
46–544（499 aa）
Fragment：UNP residues 64-544
|
Not recorded | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；290.15 K；The crystallization solution was composed of 9-11% poly(ethylene glycol) (PEG) MW 8000, 75mM MnSO4 and 100mM sodium cacodylate buffer., pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K
|
Resolution 0.74 Å R-free 0.123 |
| 4U2L Dithionite reduced cholesterol in complex with sulfite Deposited 2014-07-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | SO4 SULFATE ION × 3 SFD (S)-10-((2S,3S,4R)-5-((S)-((S)-(((2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXY-TETRAHYDROFURAN-2-YL)METHOXY)(HYDROXY)PHOSPHORYLOXY)(HYDROXY)PHOSPHORYLOXY)-2,3,4-TRIHYDROXYPENTYL)-7,8-DIMETHYL-2,4-DIOXO-2,3,4,4A-TETRAHYDROBENZO[G]PTERIDINE-5(10H)-SULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.2；293 K；PEG 8000, manganese chloride, cacodylate
|
Resolution 1.34 Å R-free 0.133 |
| 4U2S Cholesterol oxidase in the reduced state complexed with isopropanol Deposited 2014-07-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | SO4 SULFATE ION × 4 FDA DIHYDROFLAVINE-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEG 8000, manganese sulfate, cacodylate
|
Resolution 1.12 Å R-free 0.107 |
| 4U2T Cholesterol oxidase in the oxidised state complexed with isopropanol Deposited 2014-07-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | SO4 SULFATE ION × 1 FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEG 8000, manganese sulfate, cacodylate
|
Resolution 1.22 Å R-free 0.149 |
| 4XWR X-ray structure of perdeuterated Cholesterol Oxidase from Streptomyces SA-COO Deposited 2015-01-29 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.2；293 K；PEG 8K, MnSO4, sodium cacodylate
|
Resolution 1.10 Å R-free 0.107 |
| 4XXG Structure of protonated Cholesterol Oxidase from Streptomyces SA-COO Deposited 2015-01-30 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；PEG 8K, MnSO4, sodium cacodylate
|
Resolution 0.85 Å R-free 0.124 |
| 5KWF Joint X-ray Neutron Structure of Cholesterol Oxidase Deposited 2016-07-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
43–546（504 aa）
|
Not recorded | FAD FLAVIN-ADENINE DINUCLEOTIDE × 1 | Not declared |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；7% PEG 8000, 100mM MnSO4, 100mM cacodylic acid pH 5.2
|
Resolution not provided |