当前蛋白身份：P13063
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1CC1 CRYSTAL STRUCTURE OF A REDUCED, ACTIVE FORM OF THE NI-FE-SE HYDROGENASE FROM DESULFOMICROBIUM BACULATUM 提交 1999-03-03 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 S
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 NI NICKEL (II) ION × 1 FE2 FE (II) ION × 1 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.2；pH 6.2
|
分辨率 2.15 Å R-free 0.248 |
| 4KL8 High-resolution structure of anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-07 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 S
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；22-27% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, under air, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.52 Å R-free 0.171 |
| 4KL8 High-resolution structure of anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-07 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 T
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 5 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；22-27% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, under air, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.52 Å R-free 0.171 |
| 4KN9 High-resolution structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 S
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.40 Å R-free 0.168 |
| 4KN9 High-resolution structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-09 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 T
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 4 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.40 Å R-free 0.168 |
| 4KO1 High X-ray dose structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 S
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 3 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.55 Å R-free 0.184 |
| 4KO1 High X-ray dose structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-11 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 T
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 4 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.55 Å R-free 0.184 |
| 4KO2 Low X-ray dose structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 S
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 4 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.60 Å R-free 0.194 |
| 4KO2 Low X-ray dose structure of H2-activated anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-11 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 T
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 4 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.60 Å R-free 0.194 |
| 4KO3 Low X-ray dose structure of anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 S
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.70 Å R-free 0.201 |
| 4KO3 Low X-ray dose structure of anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-11 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 T
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 5 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 1.70 Å R-free 0.201 |
| 4KO4 High X-ray dose structure of anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 S
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 2.00 Å R-free 0.172 |
| 4KO4 High X-ray dose structure of anaerobically purified Dm. baculatum [NiFeSe]-hydrogenase after crystallization under air 提交 2013-05-11 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 T
33–315（283 aa）
|
未记录 | SF4 IRON/SULFUR CLUSTER × 3 CA CALCIUM ION × 5 GOL GLYCEROL × 2 FCO CARBONMONOXIDE-(DICYANO) IRON × 1 NI NICKEL (II) ION × 1 H2S HYDROSULFURIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；25% PEG 4000, 0.2M CaCl2, 0.1M Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 2.00 Å R-free 0.172 |