Current Protein Identity：P14151
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2LGF Solution structure of Ca2+/calmodulin complexed with a peptide representing the calmodulin-binding domain of L-selectin Deposited 2011-07-25 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
349–363（15 aa）
Fragment：sequence database residues 349-363
|
Not recorded | CA CALCIUM ION × 4 | SOLUTION NMR |
NMR measurement conditions
pH 6.8；303 K；Ionic strength (raw mmCIF value) 0.1；Pressure ambient
NMR measurement conditions
pH 6.8；303 K；Ionic strength (raw mmCIF value) 0.3；Pressure ambient
NMR sample composition
0.5-0.8 mM [U-13C; U-15N] protein_1, 0.5-0.8 mM protein_2, 4 mM CALCIUM ION, 0.5 mM DSS, 100 mM potassium chloride, 0.03 % sodium azide, 20 mM Bis-Tris, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5-0.8 mM [U-2H; U-15N] protein_1, 0.5-0.8 mM protein_2, 4 mM CALCIUM ION, 0.5 mM DSS, 100 mM potassium chloride, 0.03 % sodium azide, 20 mM Bis-Tris, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5-0.8 mM [1H/13C-methyl Met; U-2H; U-15N] protein_1, 0.5-0.8 mM protein_2, 4 mM CALCIUM ION, 0.5 mM DSS, 100 mM potassium chloride, 0.03 % sodium azide, 20 mM Bis-Tris, 100% D2O | 100% D2O
NMR sample composition
0.5-0.8 mM [U-13C; U-15N] protein_1, 0.5-0.8 mM protein_2, 4 mM CALCIUM ION, 0.5 mM DSS, 300 mM potassium chloride, 0.03 % sodium azide, 20 mM Bis-Tris, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5-0.8 mM [U-13C; U-15N] protein_1, 0.5-0.8 mM protein_2, 4 mM CALCIUM ION, 0.5 mM DSS, 300 mM potassium chloride, 0.03 % sodium azide, 20 mM Bis-Tris, 16 w/v Pf1 phage, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 3CFW L-selectin lectin and EGF domains Deposited 2008-03-04 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
39–194（156 aa）
Fragment：EGF domain (UNP residues 39-194)
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.17 M Sodium acetate trihydrate, 0.085 M Tris-HCl, pH 8.5, 25.5 % w/v PEG 4000,15% v/v Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.248 |
| 5VC1 Crystal structure of L-selectin lectin/EGF domains Deposited 2017-03-30 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
39–195（157 aa）
Fragment：UNP residues 39-195
|
Mutation：N22Q, N139Q | PG4 TETRAETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 GOL GLYCEROL × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100 mM sodium cacodylate pH 7.5, 200 mM calcium acetate, 40% (v/v) PEG 600
|
Resolution 1.94 Å R-free 0.231 |