Current Protein Identity:P15938
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 | Assembly 1 Protein–RNA Heteromer;Protein × 40 PDB declaration: 45-meric(45) Consistent with all polymers |
Chain Q
1–1071(1071 aa)
|
Not recorded | MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE;3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
|
Resolution 10.00 Å |
| 5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 | Assembly 1 Protein–RNA Heteromer;Protein × 38 PDB declaration: 45-meric(45) Consistent with all polymers |
Chain e
1–1071(1071 aa)
|
Not recorded | GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8;10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |