5wsg

Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution

Method: ELECTRON MICROSCOPY

▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Pre-mRNA-splicing factor 8

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP8_YEAST
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–2413； UniProt 1–2413

Pre-mRNA-splicing factor SNU114

OrganismNot specified

UniProt P36048

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SN114_YEAST
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain C； PDBConstruct 1–1008； UniProt 1–1008

Pre-mRNA-splicing factor CWC21

OrganismNot specified

UniProt Q03375

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CWC21_YEAST
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain J； PDBConstruct 1–135； UniProt 1–135

Pre-mRNA-splicing factor PRP46

OrganismNot specified

UniProt Q12417

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP46_YEAST
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain O； PDBConstruct 1–451； UniProt 1–451

Pre-mRNA-processing protein 45

OrganismNot specified

UniProt P28004

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP45_YEAST
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain P； PDBConstruct 1–379； UniProt 1–379

Pre-mRNA-splicing factor SLT11

OrganismNot specified

UniProt P38241

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SLT11_YEAST
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain Q； PDBConstruct 1–364； UniProt 1–364

Pre-mRNA-splicing factor CWC2

OrganismNot specified

UniProt Q12046

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CWC2_YEAST
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain R； PDBConstruct 1–339； UniProt 1–339

Pre-mRNA-splicing factor CWC15

OrganismNot specified

UniProt Q03772

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CWC15_YEAST
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain S； PDBConstruct 1–175； UniProt 1–175

Pre-mRNA-splicing factor BUD31

OrganismNot specified

UniProt P25337

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	BUD31_YEAST
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain T； PDBConstruct 1–157； UniProt 1–157

Pre-mRNA-splicing factor CWC22

OrganismNot specified

UniProt P53333

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CWC22_YEAST
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain Z； PDBConstruct 1–577； UniProt 1–577

Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1

OrganismNot specified

UniProt Q03654

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CEF1_YEAST
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain c； PDBConstruct 1–245； UniProt 9–253 Author chain c； PDBConstruct 474–579； UniProt 482–587

Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1

OrganismNot specified

UniProt Q12309

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CLF1_YEAST
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain d； PDBConstruct 1–240； UniProt 36–275

Pre-mRNA-splicing factor SYF2

OrganismNot specified

UniProt P53277

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SYF2_YEAST
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain I； PDBConstruct 1–215； UniProt 1–215

Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1

OrganismNot specified

UniProt Q04048

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SYF1_YEAST
Isoform	—
PDB entities	20
Chains and sequence ranges	Author chain v； PDBConstruct 569–734； UniProt 569–734

Pre-mRNA-processing factor 17

OrganismNot specified

UniProt P40968

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP17_YEAST
Isoform	—
PDB entities	21
Chains and sequence ranges	Author chain n； PDBConstruct 1–455； UniProt 1–455

Pre-mRNA-processing factor 19

OrganismNot specified

UniProt P32523

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP19_YEAST
Isoform	—
PDB entities	22
Chains and sequence ranges	Author chain o； PDBConstruct 1–503； UniProt 1–503 Author chain p； PDBConstruct 1–503； UniProt 1–503 Author chain q； PDBConstruct 1–503； UniProt 1–503 Author chain r； PDBConstruct 1–503； UniProt 1–503

Pre-mRNA-splicing factor SNT309

OrganismNot specified

UniProt Q06091

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SN309_YEAST
Isoform	—
PDB entities	23
Chains and sequence ranges	Author chain t； PDBConstruct 1–175； UniProt 1–175

Small nuclear ribonucleoprotein-associated protein B

OrganismNot specified

UniProt P40018

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RSMB_YEAST
Isoform	—
PDB entities	24
Chains and sequence ranges	Author chain F； PDBConstruct 1–196； UniProt 1–196 Author chain k； PDBConstruct 1–196； UniProt 1–196

Small nuclear ribonucleoprotein E

OrganismNot specified

UniProt Q12330

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RUXE_YEAST
Isoform	—
PDB entities	25
Chains and sequence ranges	Author chain G； PDBConstruct 1–94； UniProt 1–94 Author chain i； PDBConstruct 1–94； UniProt 1–94

Small nuclear ribonucleoprotein F

OrganismNot specified

UniProt P54999

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RUXF_YEAST
Isoform	—
PDB entities	26
Chains and sequence ranges	Author chain H； PDBConstruct 1–86； UniProt 1–86 Author chain h； PDBConstruct 1–86； UniProt 1–86

Small nuclear ribonucleoprotein G

OrganismNot specified

UniProt P40204

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RUXG_YEAST
Isoform	—
PDB entities	27
Chains and sequence ranges	Author chain K； PDBConstruct 1–77； UniProt 1–77 Author chain j； PDBConstruct 1–77； UniProt 1–77

Small nuclear ribonucleoprotein Sm D3

OrganismNot specified

UniProt P43321

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SMD3_YEAST
Isoform	—
PDB entities	28
Chains and sequence ranges	Author chain U； PDBConstruct 1–101； UniProt 1–101 Author chain l； PDBConstruct 1–101； UniProt 1–101

Small nuclear ribonucleoprotein Sm D1

OrganismNot specified

UniProt Q02260

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SMD1_YEAST
Isoform	—
PDB entities	29
Chains and sequence ranges	Author chain V； PDBConstruct 1–146； UniProt 1–146 Author chain m； PDBConstruct 1–146； UniProt 1–146

Small nuclear ribonucleoprotein Sm D2

OrganismNot specified

UniProt Q06217

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SMD2_YEAST
Isoform	—
PDB entities	30
Chains and sequence ranges	Author chain W； PDBConstruct 1–110； UniProt 1–110 Author chain g； PDBConstruct 1–110； UniProt 1–110

;U2 small nuclear ribonucleoprotein B'' ;

OrganismNot specified

UniProt P40567

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MSL1_YEAST
Isoform	—
PDB entities	31
Chains and sequence ranges	Author chain X； PDBConstruct 1–111； UniProt 1–111

;U2 small nuclear ribonucleoprotein A' ;

OrganismNot specified

UniProt Q08963

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RU2A_YEAST
Isoform	—
PDB entities	32
Chains and sequence ranges	Author chain Y； PDBConstruct 1–238； UniProt 1–238

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

OrganismNot specified

UniProt P15938

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor 18 × 1 （P33411） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP16_YEAST
Isoform	—
PDB entities	34
Chains and sequence ranges	Author chain e； PDBConstruct 1–1071； UniProt 1–1071

Pre-mRNA-splicing factor 18

OrganismNot specified

UniProt P33411

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 38 RNA 7	Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Pre-mRNA-splicing factor CWC21 × 1 （Q03375） Pre-mRNA-splicing factor PRP46 × 1 （Q12417） Pre-mRNA-processing protein 45 × 1 （P28004） Pre-mRNA-splicing factor SLT11 × 1 （P38241） Pre-mRNA-splicing factor CWC2 × 1 （Q12046） Pre-mRNA-splicing factor CWC15 × 1 （Q03772） Pre-mRNA-splicing factor BUD31 × 1 （P25337） Pre-mRNA-splicing factor CWC22 × 1 （P53333） 5'-exon × 1 5'-intron-lariat × 1 U5 snRNA × 1 Saccharomyces cerevisiae S288c SNR6 snRNA × 1 RNA (91-MER) × 1 3'-intron-lariat × 1 Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1 × 1 （Q03654） Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1 × 1 （Q12309） Pre-mRNA-splicing factor SYF2 × 1 （P53277） Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1 × 1 （Q04048） Pre-mRNA-processing factor 17 × 1 （P40968） Pre-mRNA-processing factor 19 × 4 （P32523） Pre-mRNA-splicing factor SNT309 × 1 （Q06091） Small nuclear ribonucleoprotein-associated protein B × 2 （P40018） Small nuclear ribonucleoprotein E × 2 （Q12330） Small nuclear ribonucleoprotein F × 2 （P54999） Small nuclear ribonucleoprotein G × 2 （P40204） Small nuclear ribonucleoprotein Sm D3 × 2 （P43321） Small nuclear ribonucleoprotein Sm D1 × 2 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 2 （Q06217） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） 3'-exon-intron × 1 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 × 1 （P15938） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 6 ZINC ION × 6	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP18_YEAST
Isoform	—
PDB entities	35
Chains and sequence ranges	Author chain f； PDBConstruct 1–251； UniProt 1–251

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id	5wsg
Deposition date deposition_date	2016-12-07
Structure title title	Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution
Keywords keywords	Catalytic Step II spliceosome, C* spliceosome, RNA BINDING PROTEIN-RNA complex; RNA BINDING PROTEIN/RNA
Experimental Method method	ELECTRON MICROSCOPY

▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5wsg__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5wsg__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

5wsg__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	85.60 Å
Rg (electron density)	86.03 Å
Total Rg	85.31 Å
Atom count	76730
Residues	9866
Excluded volume	1326300 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`5wsg__assembly_1__model_1`	45-meric （45）	Success	4.1.3-1-20251215 (887e7ef)	View Download

▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (38)

Entity 1 Entity 1 polymer

Description description	Pre-mRNA-splicing factor 8
Molecular weight formula_weight	279867.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	76730

MSGLPPPPPGFEEDSDLALPPPPPPPPGYEIEELDNPMVPSSVNEDTFLPPPPPPPSNFEINAEEIVDFTLPPPPPPPGLDELETKAEKKVELHGKRKLDIGKDTFVTRKSRKRAKKMTKKAKRSNLYTPKAEMPPEHLRKIINTHSDMASKMYNTDKKAFLGALKYLPHAILKLLENMPHPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWSATWIAMRREKRDRTHFKRMRFPPFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEYVKDWLYDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRDEVTDKNYYYLFDKKSFFNGKALNNAIPGGPKFEPLYPREEEEDYNEFNSIDRVIFRVPIRSEYKVAFPHLYNSRPRSVRIPWYNNPVSCIIQNDEEYDTPALFFDPSLNPIPHFIDNNSSLNVSNTKENGDFTLPEDFAPLLAEEEELILPNTKDAMSLYHSPFPFNRTKGKMVRAQDVALAKKWFLQHPDEEYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTKLLKSLKNTKYFQQTTIDWVEAGLQLCRQGHNMLNLLIHRKGLTYLHLDYNFNLKPTKTLTTKERKKSRLGNSFHLMRELLKMMKLIVDTHVQFRLGNVDAFQLADGIHYILNHIGQLTGIYRYKYKVMHQIRACKDLKHIIYYKFNKNLGKGPGCGFWQPAWRVWLNFLRGTIPLLERYIGNLITRQFEGRSNEIVKTTTKQRLDAYYDLELRNSVMDDILEMMPESIRQKKARTILQHLSEAWRCWKANIPWDVPGMPAPIKKIIERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVKKNLGRLTRLWIKNEQERQRQIQKNGPEITPEEATTIFSVMVEWLESRSFSPIPFPPLTYKNDTKILVLALEDLKDVYASKVRLNASEREELALIEEAYDNPHDTLNRIKKYLLTQRVFKPVDITMMENYQNISPVYSVDPLEKITDAYLDQYLWYEADQRKLFPNWIKPSDSEIPPLLVYKWTQGINNLSEIWDVSRGQSAVLLETTLGEMAEKIDFTLLNRLLRLIVDPNIADYITAKNNVVINFKDMSHVNKYGLIRGLKFASFIFQYYGLVIDLLLLGQERATDLAGPANNPNEFMQFKSKEVEKAHPIRLYTRYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFENSIGYNNRKCWPKDSRMRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENAFVSVYSKNNPNLLFSMCGFEVRILPRQRMEEVVSNDEGVWDLVDERTKQRTAKAYLKVSEEEIKKFDSRIRGILMASGSTTFTKVAAKWNTSLISLFTYFREAIVATEPLLDILVKGETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGGLGMISASHILIPASDLSWSKQTDTGITHFRAGMTHEDEKLIPTIFRYITTWENEFLDSQRVWAEYATKRQEAIQQNRRLAFEELEGSWDRGIPRISTLFQRDRHTLAYDRGHRIRREFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTDVIQALGGIETILEHTLFKGTGFNSWEGLFWEKASGFEDSMQFKKLTHAQRTGLSQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESIVFDICQILDGELDVLQIESVTKETVHPRKSYKMNSSAADITMESVHEWEVSKPSLLHETNDSFKGLITNKMWFDVQLRYGDYDSHDISRYVRAKFLDYTTDNVSMYPSPTGVMIGIDLAYNMYDAYGNWFNGLKPLIQNSMRTIMKANPALYVLRERIRKGLQIYQSSVQEPFLNSSNYAELFNNDIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKTGHLFLKIIHTSVWAGQKRLSQLAKWKTAEEVSALVRSLPKEEQPKQIIVTRKAMLDPLEVHMLDFPNIAIRPTELRLPFSAAMSIDKLSDVVMKATEPQMVLFNIYDDWLDRISSYTAFSRLTLLLRALKTNEESAKMILLSDPTITIKSYHLWPSFTDEQWITIESQMRDLILTEYGRKYNVNISALTQTEIKDIILGQNIKAPSVKRQKMAELEAARSEKQNDEEAAGASTVMKTKTINAQGEEIVVVASADYESQTFSSKNEWRKSAIANTLLYLRLKNIYVSADDFVEEQNVYVLPKNLLKKFIEISDVKIQVAAFIYGMSAKDHPKVKEIKTVVLVPQLGHVGSVQISNIPDIGDLPDTEGLELLGWIHTQTEELKFMAASEVATHSKLFADKKRDCIDISIFSTPGSVSLSAYNLTDEGYQWGEENKDIMNVLSEGFEPTFSTHAQLLLSDRITGNFIIPSGNVWNYTFMGTAFNQEGDYNFKYGIPLEFYNEMHRPVHFLQFSELAGDEELEAEQIDVFS

Entity 2 Entity 2 polymer

Description description	Pre-mRNA-splicing factor SNU114
Molecular weight formula_weight	114174.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MEGDDLFDEFGNLIGVDPFDSDEEESVLDEQEQYQTNTFEGSGNNNEIESRQLTSLGSKKELGISLEHPYGKEVEVLMETKNTQSPQTPLVEPVTERTKLQEHTIFTQLKKNIPKTRYNRDYMLSMANIPERIINVGVIGPLHSGKTSLMDLLVIDSHKRIPDMSKNVELGWKPLRYLDNLKQEIDRGLSIKLNGSTLLCTDLESKSRMINFLDAPGHVNFMDETAVALAASDLVLIVIDVVEGVTFVVEQLIKQSIKNNVAMCFVINKLDRLILDLKLPPMDAYLKLNHIIANINSFTKGNVFSPIDNNIIFASTKLGFTFTIKEFVSYYYAHSIPSSKIDDFTTRLWGSVYYHKGNFRTKPFENVEKYPTFVEFILIPLYKIFSYALSMEKDKLKNLLRSNFRVNLSQEALQYDPQPFLKHVLQLIFRQQTGLVDAITRCYQPFELFDNKTAHLSIPGKSTPEGTLWAHVLKTVDYGGAEWSLVRIYSGLLKRGDTVRILDTSQSESRQKRQLHDISKTETSNEDEDEDDETPSCEVEEIGLLGGRYVYPVHEAHKGQIVLIKGISSAYIKSATLYSVKSKEDMKQLKFFKPLDYITEAVFKIVLQPLLPRELPKLLDALNKISKYYPGVIIKVEESGEHVILGNGELYMDCLLYDLRASYAKIEIKISDPLTVFSESCSNESFASIPVSNSISRLGEENLPGLSISVAAEPMDSKMIQDLSRNTLGKGQNCLDIDGIMDNPRKLSKILRTEYGWDSLASRNVWSFYNGNVLINDTLPDEISPELLSKYKEQIIQGFYWAVKEGPLAEEPIYGVQYKLLSISVPSDVNIDVMKSQIIPLMKKACYVGLLTAIPILLEPIYEVDITVHAPLLPIVEELMKKRRGSRIYKTIKVAGTPLLEVRGQVPVIESAGFETDLRLSTNGLGMCQLYFWHKIWRKVPGDVLDKDAFIPKLKPAPINSLSRDFVMKTRRRKGISTGGFMSNDGPTLEKYISAELYAQLRENGLVP

Entity 3 Entity 3 polymer

Description description	Pre-mRNA-splicing factor CWC21
Molecular weight formula_weight	15793.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	J

MSYNGIGLKSAKGSSTSGHVQRSLASNNRRRPQGSQQQRQQRQNAIKKASHDKASRPLAVQKQIETHMEKREIEVQVSELRDRLEEEETLSEEQIDKKCEALRAKLTNEWQEQQRMSSLYTPRKARLTEEQHRHE

Entity 4 Entity 4 polymer

Description description	Pre-mRNA-splicing factor PRP46
Molecular weight formula_weight	50771.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O

MDGNDHKVENLGDVDKFYSRIRWNNQFSYMATLPPHLQSEMEGQKSLLMRYDTYRKESSSFSGEGKKVTLQHVPTDFSEASQAVISKKDHDTHASAFVNKIFQPEVAEELIVNRYEKLLSQRPEWHAPWKLSRVINGHLGWVRCVAIDPVDNEWFITGSNDTTMKVWDLATGKLKTTLAGHVMTVRDVAVSDRHPYLFSVSEDKTVKCWDLEKNQIIRDYYGHLSGVRTVSIHPTLDLIATAGRDSVIKLWDMRTRIPVITLVGHKGPINQVQCTPVDPQVVSSSTDATVRLWDVVAGKTMKVLTHHKRSVRATALHPKEFSVASACTDDIRSWGLAEGSLLTNFESEKTGIINTLSINQDDVLFAGGDNGVLSFYDYKSGHKYQSLATREMVGSLEGERSVLCSTFDKTGLRLITGEADKSIKIWKQDETATKESEPGLAWNPNLSAKRF

Entity 5 Entity 5 polymer

Description description	Pre-mRNA-processing protein 45
Molecular weight formula_weight	42548.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

MFSNRLPPPKHSQGRVSTALSSDRVEPAILTDQIAKNVKLDDFIPKRQSNFELSVPLPTKAEIQECTARTKSYIQRLVNAKLANSNNRASSRYVTETHQAPANLLLNNSHHIEVVSKQMDPLLPRFVGKKARKVVAPTENDEVVPVLHMDGSNDRGEADPNEWKIPAAVSNWKNPNGYTVALERRVGKALDNENNTINDGFMKLSEALENADKKARQEIRSKMELKRLAMEQEMLAKESKLKELSQRARYHNGTPQTGAIVKPKKQTSTVARLKELAYSQGRDVSEKIILGAAKRSEQPDLQYDSRFFTRGANASAKRHEDQVYDNPLFVQQDIESIYKTNYEKLDEAVNVKSEGASGSHGPIQFTKAESDDKSDNYGA

Entity 6 Entity 6 polymer

Description description	Pre-mRNA-splicing factor SLT11
Molecular weight formula_weight	40988.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MNDEINEPPPNICEQCLGDEANIRMTKIPQGSECKICTLPFTLYHFKTSKRSNNIIKTLICVRCATQRNICQCCMLDSRWHIPIQLRDHLISLVNEENVMTEEAKNDMMKRFLSLKNVKLGGAQITSDPSEADNIVDKLKNILLRATSDGPSTPLIKNTTALYKNEKGANEVKNLEKYASVDISHILKKLPLNESFLKNPSTKSFFLYNIDASIPEWKITDTVSQLLGIKKWKDGNSLSLIVNHKAKCGGLRFQSSELGERFVSKISETLVTPKGLKRGVLLIDRFRIFIIPWSSGFSAASFGTNTAENIKLSLSLNKLIQLELGLSFPTKSTDNAKNDKKKTSKKVHKDRSKKSKPRANKLTI

Entity 7 Entity 7 polymer

Description description	Pre-mRNA-splicing factor CWC2
Molecular weight formula_weight	38486.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MTSWRDKSAKVQVKESELPSSIPAQTGLTFNIWYNKWSQGFAGNTRFVSPFALQPQLHSGKTRGDNDGQLFFCLFFAKGMCCLGPKCEYLHHIPDEEDIGKLALRTEVLDCFGREKFADYREDMGGIGSFRKKNKTLYVGGIDGALNSKHLKPAQIESRIRFVFSRLGDIDRIRYVESKNCGFVKFKYQANAEFAKEAMSNQTLLLPSDKEWDDRREGTGLLVKWANEDPDPAAQKRLQEELKLESLNMMVHLINNNTNSAGTEVNNKNNERLDRTFPEASVDNVKKRLLPLDNGMESDDFIEKLKKVKKNISRENISSKPSVGKLGGPLLDYLSSDED

Entity 8 Entity 8 polymer

Description description	Pre-mRNA-splicing factor CWC15
Molecular weight formula_weight	19975.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S

MTTSHRPQLEARSGAKAAAYTPTGIEHARLLPGHTTLKYRKFKEEENLRANCAQEDRSNDKSLEEAVMNEEKQDVVGSGNLQETRSEKDQKDSLQELLVTQKNKVEDKAELEGNEQLKGGNSSRRSWRKGTAFGRHKVTKETNIKEHATKKSASGYINDMTKSEYHQEFLHKHVR

Entity 9 Entity 9 polymer

Description description	Pre-mRNA-splicing factor BUD31
Molecular weight formula_weight	18484.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T

MPRIKTRRSKPAPDGFEKIKPTLTDFEIQLRDAQKDKSSKLAAKSNEQLWEIMQLHHQRSRYIYTLYYKRKAISKDLYDWLIKEKYADKLLIAKWRKTGYEKLCCLRCIQKNETNNGSTCICRVPRAQLEEEARKKGTQVSFHQCVHCGCRGCASTD

Entity 10 Entity 10 polymer

Description description	Pre-mRNA-splicing factor CWC22
Molecular weight formula_weight	67386.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Z

MSTATIQDEDIKFQRENWEMIRSHVSPIISNLTMDNLQESHRDLFQVNILIGRNIICKNVVDFTLNKQNGRLIPALSALIALLNSDIPDIGETLAKELMLMFVQQFNRKDYVSCGNILQCLSILFLYDVIHEIVILQILLLLLEKNSLRLVIAVMKICGWKLALVSKKTHDMIWEKLRYILQTQELSSTLRESLETLFEIRQKDYKSGSQGLFILDPTSYTVHTHSYIVSDEDEANKELGNFEKCENFNELTMAFDTLRQKLLINNTSDTNEGSNSQLQIYDMTSTNDVEFKKKIYLVLKSSLSGDEAAHKLLKLKIANNLKKSVVDIIIKSSLQESTFSKFYSILSERMITFHRSWQTAYNETFEQNYTQDIEDYETDQLRILGKFWGHLISYEFLPMDCLKIIKLTEEESCPQGRIFIKFLFQELVNELGLDELQLRLNSSKLDGMFPLEGDAEHIRYSINFFTAIGLGLLTEDMRSRLTIIQEVEDAEEEEKKLREEEELEKLRKKARESQPTQGPKIHESRLFLQKDTRENSRSRSPFTVETRKRARSRTPPRGSRNHRNRSRTPPARRQRHR

Entity 11 Entity 11 polymer

Description description	5'-exon
Molecular weight formula_weight	4112.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	B

AUAAAUUUUUAAG

Entity 12 Entity 12 polymer

Description description	5'-intron-lariat
Molecular weight formula_weight	4694.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	N

GUAUGUAUUUAUUUU

Entity 13 Entity 13 polymer

Description description	U5 snRNA
Molecular weight formula_weight	68643.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	D

AAGCAGCUUUACAGAUCAAUGGCGGAGGGAGGUCAACAUCAAGAACUGUGGGCCUUUUAUUGCCUAUAGAACUUAUAACGAACAUGGUUCUUGCCUUUUACCAGAACCAUCCGGGUGUUGUCUCCAUAGAAACAGGUAAAGCUGUCCGUUACUGUGGGCUUGCCAUAUUUUUUGGAACUUUUCUGCCCUUUUUCUCAAUGAGUAAGGAGGGCGU

Entity 14 Entity 14 polymer

Description description	Saccharomyces cerevisiae S288c SNR6 snRNA
Molecular weight formula_weight	35883.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	E

GUUCGCGAAGUAACCCUUCGUGGACAUUUGGUCAAUUUGAAACAAUACAGAGAUGAUCAGCAGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUU

Entity 15 Entity 15 polymer

Description description	RNA (91-MER)
Molecular weight formula_weight	376267.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	L

ACGAAUCUCUUUGCCUUUUGGCUUAGAUCAAGUGUAGUAUCUGUUCUUUUCAGUGUAACAACUGAAAUGACCUCAAUGAGGCUCAUUACCUUUUAAUUUGUUACAAUACACAUUUUUUGGCACCCAAAAUAAUAAAAUGGACGGGAAGAGACUUUUUAAGCAAGUUGUUUUCCGCUAAUGUCAGGUCUCACUACUUUUUGCUGCUAUUUUUCUUCGCUCAUGGUUUCUUCAUAAGGCGUUUUUAUGAUGGUUUUUCGAAAUUGGUUUUUGAGACGACGGUUGCUCAAGGUUAUUGUUUUUGUUUUCUUCUGGUUGUUUUCUAUUUUCUUUUUUUUAGCUUUCUGUUUCUCCCUUAGUUUGGCUUUUUGCUUCAUACUCUUCCCUGUCUUUCCGAGCCGUUUAUGUCCAACGCGGGAUUUGGUUUUUCUUUAUCGAUGGGAAGAAAUGGUGCUAUAGUAGGUUGGGAGAUAAUAUUUAUGGUAUGGGGUGCUAGUGCGGAUGGGGCGCUCUUAUUGUUGAUUUCUUCGCUCGUCUUCUUUUUCUGGUGGCGCUGCAAGAGGAAGUUUUUCGACUUUGUUAUGAUUUUUGGUUUGCAAGGAAAGGUGUCUUACGAUUCUUUUUUUGAUGUAAUAGGAUAAGCUUGCUUAUCCCCCAAGUAUCGGCCAAAGUUGUUGAUUUUCCUUUUGAAGUGUCCUCGGUUUGAGGGGGUGUAGGGUGGGGUUGGUCUACAAUAAGAGUGUUCCAUUGUUAACGUGCUGGCGUCUUUUACUAUAUUUUUUUUCCCAGUUUAUUUUGUGCUUAUUUUCUCAUUGAGGAGAAGGAGCUCUUCUCGCAGGAUAUAAAUGGAGGUUUGCUAAAGGGGAGGAGAUGUGUUUGUGAGAAUACUGCUGAGAGAGUUCUGGAAGAGAAAAAAAGGAGGCAAUGGAAGGCGUUUGCUGGGAAAAGAGAAGAGCCAUGACUGCAUCUGUUGUUUCAAGGCCAGUUUUAUUAACCGCCUAUGUCAUAGAGGCGUUUUUUUUGGAGGGAUUUGAAGAAUGCCGGCGGCAUCAAGAAACGGACUUGAUGGUUGACGCCUGUUUUUAAAGUUAGAGACGUCGCGACCCUCGCACUUGUGGAGUCGUUCUUGACUUUUACUUUGGUCGCUUGAUGUUUCUCUCGUCUUCCCGUUCGCUCUU

Entity 16 Entity 16 polymer

Description description	3'-intron-lariat
Molecular weight formula_weight	7301.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	M

UUUUAAGAACUAGAUACUAACAC

Entity 17 Entity 17 polymer

Description description	Pre-mRNA-splicing factor CEF1,Pre-mRNA-splicing factor CEF1,Cef1,Pre-mRNA-splicing factor CEF1
Molecular weight formula_weight	60160.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	c

KGGVWTNVEDQILKAAVQKYGTHQWSKVASLLQKKTARQSELRWNEYLNPKLNFTEFSKEEDAQLLDLARELPNQWRTIADMMARPAQVCVERYNRLLESEDSGGAALSTGVTDLKAGDINPNAETQMARPDNGDLEDEEKEMLAEARARLLNTQGKKATRKIRERMLEESKRIAELQKRRELKQAGINVAIKKPKKKYGTDIDYNEDIVYEQAPMPGIYDTSTEDRQIKKKFEQFERKVNRKGLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXESRMQHITQGRTSMKIQFKTAMPPTEVLLESIQSKVESIEQLQRKLQHVQPLEQQNNEMCSTLCHHSLPALIEGQRKYYADYYAYRQEIRSLEGRRKRLQAMLNSS

Entity 18 Entity 18 polymer

Description description	Pre-mRNA-splicing factor CLF1,Pre-mRNA-splicing factor CLF1,Clf1
Molecular weight formula_weight	64183.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	d

DILDLEELREYQRRKRTEYEGYLKRNRLDMGQWIRYAQFEIEQHDMRRARSIFERALLVDSSFIPLWIRYIDAELKVKCINHARNLMNRAISTLPRVDKLWYKYLIVEESLNNVEIVRSLYTKWCSLEPGVNAWNSFVDFEIRQKNWNGVREIYSKYVMAHPQMQTWLKWVRFENRHGNTEFTRSVYSLAIDTVANLQNLQIWSDMEVAKLVNSFAHWEAAQQEYERSSALYQIAIEKWPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 19 Entity 19 polymer

Description description	Pre-mRNA-splicing factor SYF2
Molecular weight formula_weight	24850.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVYSMEDVNDADESVGDTESPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAKLSYEKTLRNLATQTQNSSKQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKLVNKLAVSLQSESKKRYEARKRQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE

Entity 20 Entity 20 polymer

Description description	Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1,Pre-mRNA-splicing factor SYF1,Syf1
Molecular weight formula_weight	78040.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	v

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKTKMAYNTVIDLRLITPAMAENFALFLQNHYEVMESFQVYEKTIPLFPPEIQYELWIEYLEVATSHQLSSLSPEHIRFLFEKALKNLCSNGIDCKTIFIAYSVFEERISGLISKSIEILRRGAVIGTVSVSTHLESRLQLWRMCISKAESTLGPSVTRELYQECIQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

Entity 21 Entity 21 polymer

Description description	Pre-mRNA-processing factor 17
Molecular weight formula_weight	52128.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	n

MGLVDGYDTSSDSDLNFDEGKSVHEKKNGNLHEDTSYEPSSNNIHKRKSHFTKSELKRRRKTRKGDGPWGSWSSSDDETSQASETQKEDQDIFVHALAEDNLDSEQIEVEEVSHFYGKSEKDYQGRGYLYPPNDVDVDLREERISFRCYLPKKVIRNYPGHPEGTTALKFLPKTGHLILSGGNDHTIKIWDFYHDYECLRDFQGHNKPIKALRFTEDCQSFLSSSFDRSVKIWDTETGKVKTRLHLNSTPADVESRPTNPHEFIVGLSNSKILHYDDRVSENQGLVQTYDHHLSSILALKYFPDGSKFISSSEDKTVRIWENQINVPIKQISDTAQHSMPFLNVHPSQNYFCAQSMDNRIYSFSLKPKYKRHPKKIFKGHSSAGYGISLAFSGDGRYICSGDSKSRLFTWDWNTSRLLNNIKIPGNKPITQVDWHPQETSKVICSGAAGKIYVCD

Entity 22 Entity 22 polymer

Description description	Pre-mRNA-processing factor 19
Molecular weight formula_weight	56629.8 kDa
EC 编号 ec_number	6.3.2.-
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	o,p,q,r

MLCAISGKVPRRPVLSPKSRTIFEKSLLEQYVKDTGNDPITNEPLSIEEIVEIVPSAQQASLTESTNSATLKANYSIPNLLTSLQNEWDAIMLENFKLRSTLDSLTKKLSTVMYERDAAKLVAAQLLMEKNEDSKDLPKSSQQAVAITREEFLQGLLQSSRDFVARGKLKAPKWPILKNLELLQAQNYSRNIKTFPYKELNKSMYYDKWVCMCRCEDGALHFTQLKDSKTITTITTPNPRTGGEHPAIISRGPCNRLLLLYPGNQITILDSKTNKVLREIEVDSANEIIYMYGHNEVNTEYFIWADNRGTIGFQSYEDDSQYIVHSAKSDVEYSSGVLHKDSLLLALYSPDGILDVYNLSSPDQASSRFPVDEEAKIKEVKFADNGYWMVVECDQTVVCFDLRKDVGTLAYPTYTIPEFKTGTVTYDIDDSGKNMIAYSNESNSLTIYKFDKKTKNWTKDEESALCLQSDTADFTDMDVVCGDGGIAAILKTNDSFNIVALTP

Entity 23 Entity 23 polymer

Description description	Pre-mRNA-splicing factor SNT309
Molecular weight formula_weight	20741.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	t

MDGLSFVDKGKIPDGYKNEIDQLVKKEFANIKREPVHPEIRGILAKRKGADNSVSTLTNALYTEYLKQRNNKKRRTPDFNDDDDTLFLEEYRRKYPRIDTSRYIPNESSEVSLLGIVDSYLKHQEIVLDTLLPQTVSNQWRINNDYIRQTCTIVEEMNIQQRKQINDLEIYRKRL

Entity 24 Entity 24 polymer

Description description	Small nuclear ribonucleoprotein-associated protein B
Molecular weight formula_weight	22427.0 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	k,F

MSKIQVAHSSRLANLIDYKLRVLTQDGRVYIGQLMAFDKHMNLVLNECIEERVPKTQLDKLRPRKDSKDGTTLNIKVEKRVLGLTILRGEQILSTVVEDKPLLSKKERLVRDKKEKKQAQKQTKLRKEKEKKPGKIAKPNTANAKHTSSNSREIAQPSSSRYNGGNDNIGANRSRFNNEAPPQTRKFQPPPGFKRK

Entity 25 Entity 25 polymer

Description description	Small nuclear ribonucleoprotein E
Molecular weight formula_weight	10385.1 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	i,G

MSNKVKTKAMVPPINCIFNFLQQQTPVTIWLFEQIGIRIKGKIVGFDEFMNVVIDEAVEIPVNSADGKEDVEKGTPLGKILLKGDNITLITSAD

Entity 26 Entity 26 polymer

Description description	Small nuclear ribonucleoprotein F
Molecular weight formula_weight	9669.9 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	h,H

MSESSDISAMQPVNPKPFLKGLVNHRVGVKLKFNSTEYRGTLVSTDNYFNLQLNEAEEFVAGVSHGTLGEIFIRCNNVLYIRELPN

Entity 27 Entity 27 polymer

Description description	Small nuclear ribonucleoprotein G
Molecular weight formula_weight	8490.8 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	j,K

MVSTPELKKYMDKKILLNINGSRKVAGILRGYDIFLNVVLDDAMEINGEDPANNHQLGLQTVIRGNSIISLEALDAI

Entity 28 Entity 28 polymer

Description description	Small nuclear ribonucleoprotein Sm D3
Molecular weight formula_weight	11240.1 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	l,U

MTMNGIPVKLLNEAQGHIVSLELTTGATYRGKLVESEDSMNVQLRDVIATEPQGAVTHMDQIFVRGSQIKFIVVPDLLKNAPLFKKNSSRPMPPIRGPKRR

Entity 29 Entity 29 polymer

Description description	Small nuclear ribonucleoprotein Sm D1
Molecular weight formula_weight	16296.8 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	m,V

MKLVNFLKKLRNEQVTIELKNGTTVWGTLQSVSPQMNAILTDVKLTLPQPRLNKLNSNGIAMASLYLTGGQQPTASDNIASLQYINIRGNTIRQIILPDSLNLDSLLVDQKQLNSLRRSGQIANDPSKKRRRDFGAPANKRPRRGL

Entity 30 Entity 30 polymer

Description description	Small nuclear ribonucleoprotein Sm D2
Molecular weight formula_weight	12876.1 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	g,W

MSSQIIDRPKHELSRAELEELEEFEFKHGPMSLINDAMVTRTPVIISLRNNHKIIARVKAFDRHCNMVLENVKELWTEKKGKNVINRERFISKLFLRGDSVIVVLKTPVE

Entity 31 Entity 31 polymer

Description description	;U2 small nuclear ribonucleoprotein B'' ;
Molecular weight formula_weight	12850.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	X

MVEPARKKQRIDRDTHHTVAEPVTEAKNTLYVSQLNEKINMQRLRVNLFLLFATFGEVLKVSMNFKKQRGQAFITMRTIDQASLAQISLNGERFFGKPLKVEFSKSETKTL

Entity 32 Entity 32 polymer

Description description	;U2 small nuclear ribonucleoprotein A' ;
Molecular weight formula_weight	27232.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Y

MKFTPSIVIDAPQYYVDHFNGKYNVDKCVILRDLQLETDSESMPSSLKHLTKPTHILDLTNNDLIMIPDLSRRDDIHTLLLGRNNIVEVDGRLLPMNVQNLTLSNNSIRRFEDLQRLRRAPRTLKNLTLIGNQVCHLANYREHVLRLVPHLETLDFQNVTAEERKSAMSFPRQADGDTLGPVNTAIRDNGSRDKTMEIMNLVVSKMTVERRNELKKQLAEATSLEEIARLEKLLSGGV

Entity 33 Entity 33 polymer

Description description	3'-exon-intron
Molecular weight formula_weight	4349.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	b

UUAUAGUUUUUUUA

Entity 34 Entity 34 polymer

Description description	Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
Molecular weight formula_weight	121815.2 kDa
EC 编号 ec_number	3.6.4.13
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	e

MGHSGREERIKDIFKELTSKELTPGLLLTLQKLAQKPNTNLEQFIASCKALTKLSSNNPIIFNELLELLKNKSEEDSTGPKKIAPSINKRKKFKIQLDLDDNEDELDSPVQKKPAPTRTLFKRIDKLKAKQLRQYSPTVKDPSPNSEQQTQNGHAETKDYEPTRSEVVEEDREWYDNDDDYGNLVPEPLSELPEEAKLLPVIRNIDNDDALRNTVQLYPIPLKQRMEWIPPFLSKFALENKVPTSIIIGSISETSSQVSALSMVNPFRNPDSEFSANAKRGSKLVALRRINMEHIQQSRDNTTVLNTAMGEVLGLENNNKAKDKSNQKICDDTALFTPSKDDIKHTKEQLPVFRCRSQLLSLIRENQVVVIIGETGSGKTTQLAQYLYEEGYANDRGKSIVVTQPRRVAAISVAKRVAMEMQVPLGKEVGYSIRFEDVTDSECTKLKFVTDGILLRETLLDDTLDKYSCVIIDEAHERSLNTDILLGFFKILLARRRDLKLIITSATMNAKKFSAFFGNAPQFTIPGRTFPVQTIYTSNPVQDYVEAAVSQAVKIHLANDCSSGDILIFMTGQEDIETTFDTLQEKFLQVYSKKFGTANFEEINDIEILPIYSALPADLQFKIFQDLHGTKRKIIIATNIAETSLTIKGIRYVIDCGYSKLKVYNPKIGLDSLVITPISKANADQRSGRAGRTAPGTAYRLYTEDTFKEDMYLQTIPEIQRTNLSNTLLLLKSLDVTDELSKFPFIDKPPLQTFLSSLYELWFIGAIDTSGQLTPLGLQMAKFPLQPSLSKILLIAVRNGCSDEMLTIVSMLSVPQVFYRPKERQKEADIARNKFFIAKSDHLTLLNVFEQWRANNFSSHWCNKHFVQYKSLVRARDIRDQLLTILKSQKIPVISSGKDWDIIKKCICSGFAHQAAKITGLRNYVHLKTGVSVQLHPTSALHGLGDLPPYVVYHELLMTSKEYICCVTSVDPFWLMEYGGLLYDIKRIKNDQEATTTGLFGEHYEHTLDKVEDDIDINIRRCKDMRDSVIQELKMTDNSNKEDKKQKTKKQNILNGKENSMKPFKRRKPFF

Entity 35 Entity 35 polymer

Description description	Pre-mRNA-splicing factor 18
Molecular weight formula_weight	28414.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	f

MDLDLASILKGEISKKKKELANSKGVQPPCTEKFQPHESANIDETPRQVEQESTDEENLSDNQSDDIRTTISKLENRPERIQEAIAQDKTISVIIDPSQIGSTEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDTKKALFPLLLQLRRNQLAPDLLISLATVLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQGGRNAANIMIDERTRLWITSIKRLITFEEWYTSNHDSLA

Entity 36 Entity 36 non-polymer

Description description	GUANOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	523.2 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 37 Entity 37 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	6

Entity 38 Entity 38 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	6

▼

6. Fold Classification (SCOP + CATH) 19 domains

CATH v4.4 (19 domains)

Domain ID domain_id	5wsgG00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgH00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgK00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgO00
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	5wsgU00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgV00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgW00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgX00
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily	330 — RRM (RNA recognition motif) domain

Domain ID domain_id	5wsgZ01
Class class	1 — Mainly Alpha
Architecture architecture	25 — Alpha Horseshoe
Topology topology	40 — Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Homologous superfamily homologous superfamily	180 —

Domain ID domain_id	5wsgf00
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	940 — RNA Binding Protein, Prp18; Chain A
Homologous superfamily homologous superfamily	10 — Functional domain of the splicing factor Prp18

Domain ID domain_id	5wsgg00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgh00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgi00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgj00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgl00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgm00
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	100 —

Domain ID domain_id	5wsgp01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	40 — Herpes Virus-1
Homologous superfamily homologous superfamily	10 — Zinc/RING finger domain, C3HC4 (zinc finger)

Domain ID domain_id	5wsgq01
Class class	2 — Mainly Beta
Architecture architecture	130 — 7 Propeller
Topology topology	10 — Methylamine Dehydrogenase; Chain H
Homologous superfamily homologous superfamily	10 — YVTN repeat-like/Quinoprotein amine dehydrogenase

Domain ID domain_id	5wsgr01
Class class	3 — Alpha Beta
Architecture architecture	30 — 2-Layer Sandwich
Topology topology	40 — Herpes Virus-1
Homologous superfamily homologous superfamily	10 — Zinc/RING finger domain, C3HC4 (zinc finger)

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Resolution resolution	4.000 Å
Low-resolution limit resolution_low	— Å
R factor r_factor	—
R_work r_work	—
R_free r_free	—

Resolution resolution	4.00 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	27558

Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution

1. Protein Identity and Related Structures Protein Identity & Related Structures

Pre-mRNA-splicing factor 8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor SNU114

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor CWC21

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor PRP46

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-processing protein 45

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor SLT11

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor CWC2

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor CWC15

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor BUD31

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor CWC22

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer