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5nrl

Structure of a pre-catalytic spliceosome

Method: ELECTRON MICROSCOPY Dmax: 277.3 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

U6 snRNA-associated Sm-like protein LSm3

OrganismNot specified

UniProt P57743

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain 3； UniProt 1–89 Not recorded U2 snRNA × 1 U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3BW1 Crystal structure of homomeric yeast Lsm3 exhibiting novel octameric ring organisation Deposited 2008-01-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric
Chain A 1–89（89 aa）
Chain B 1–89（89 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 16 SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；1.75M lithium sulfate, 8% (v/v) 2-methyl-2,4-pentanediol, 0.1M imidazole (pH 7.0), 10mM uridine-5-monophosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.50 Å R-free 0.243
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain d 1–89（89 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4C8Q Crystal structure of the yeast Lsm1-7-Pat1 complex Deposited 2013-10-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–89（89 aa） Fragment：RESIDUES 1-89
Not recorded CO COBALT (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM HEPES PH 7.0, 20% MPD, 10 MM HEXAMINE COBALT(III) CHLORIDE
Resolution 3.70 Å R-free 0.295
4C92 Crystal structure of the yeast Lsm1-7 complex Deposited 2013-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–89（89 aa） Fragment：RESIDUES 1-89
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM MES PH 6.0, 40% MPD
Resolution 2.30 Å R-free 0.259
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–89（89 aa）
Mutation：C37S,C63S Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain J 1–89（89 aa）
Mutation：C37S,C63S Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–89（89 aa）
Mutation：C37S,C63S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain J 1–89（89 aa）
Mutation：C37S,C63S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain C 1–89（89 aa）
Mutation：C37S,C63S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain J 1–89（89 aa）
Mutation：C37S,C63S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain J 1–89（89 aa）
Mutation：C37S,C63S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain C 1–89（89 aa）
Mutation：C37S,C63S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein–RNA Heteromer；Protein × 14 PDB declaration: hexadecameric
Chain C 1–89（89 aa）
Chain J 1–89（89 aa）
Mutation：C37S,C63S Mutation：C37S,C63S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain C 1–89（89 aa）
Mutation：C37S,C63S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain J 1–89（89 aa）
Mutation：C37S,C63S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
4N0A Crystal structure of Lsm2-3-Pat1C complex from Saccharomyces cerevisiae Deposited 2013-10-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain A 1–89（89 aa）
Chain B 1–89（89 aa）
Chain E 1–89（89 aa）
Chain F 1–89（89 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；288 K；0.1M Tris, 12% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K
Resolution 3.15 Å R-free 0.300
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain 3 1–89（89 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5VSU Structure of yeast U6 snRNP with 2'-phosphate terminated U6 RNA Deposited 2017-05-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain C 1–89（89 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；289 K；0.2 M NH4F 0.1 M HEPES pH 7.4 0.01 M MgCl2 18 % PEG 3,350
Resolution 3.10 Å R-free 0.298
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain r 1–89（89 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain r 1–89（89 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6ASO Structure of yeast U6 snRNP with 3'-phosphate terminated U6 RNA Deposited 2017-08-25 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain C 1–89（89 aa）
Not recorded K POTASSIUM ION × 2 MN MANGANESE (II) ION × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；280 mM sodium potassium tartrate 22.5 % glycerol 17.5 % PEG 3,350 1 mM manganese chloride
Resolution 2.71 Å R-free 0.247

15 other PDB entries and 21 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LSM3_YEAST
Isoform —
PDB entities 2
Chains and sequence ranges Author chain 3； PDBConstruct 1–89； UniProt 1–89

U6 snRNA-associated Sm-like protein LSm7

OrganismNot specified

UniProt P53905

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain 7； UniProt 1–115 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain g 1–115（115 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4C8Q Crystal structure of the yeast Lsm1-7-Pat1 complex Deposited 2013-10-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 1–115（115 aa） Fragment：RESIDUES 1-115
Not recorded CO COBALT (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM HEPES PH 7.0, 20% MPD, 10 MM HEXAMINE COBALT(III) CHLORIDE
Resolution 3.70 Å R-free 0.295
4C92 Crystal structure of the yeast Lsm1-7 complex Deposited 2013-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–115（115 aa） Fragment：RESIDUES 1-115
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM MES PH 6.0, 40% MPD
Resolution 2.30 Å R-free 0.259
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain M 1–115（115 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain M 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain F 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain M 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain M 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain F 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein–RNA Heteromer；Protein × 14 PDB declaration: hexadecameric
Chain F 1–115（115 aa）
Chain M 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain F 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain M 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain 7 1–115（115 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5VSU Structure of yeast U6 snRNP with 2'-phosphate terminated U6 RNA Deposited 2017-05-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain G 1–115（115 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；289 K；0.2 M NH4F 0.1 M HEPES pH 7.4 0.01 M MgCl2 18 % PEG 3,350
Resolution 3.10 Å R-free 0.298
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain y 1–115（115 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain y 1–115（115 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6ASO Structure of yeast U6 snRNP with 3'-phosphate terminated U6 RNA Deposited 2017-08-25 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain G 1–115（115 aa）
Not recorded K POTASSIUM ION × 2 MN MANGANESE (II) ION × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；280 mM sodium potassium tartrate 22.5 % glycerol 17.5 % PEG 3,350 1 mM manganese chloride
Resolution 2.71 Å R-free 0.247

13 other PDB entries and 19 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LSM7_YEAST
Isoform —
PDB entities 6
Chains and sequence ranges Author chain 7； PDBConstruct 1–115； UniProt 1–115

U6 snRNA-associated Sm-like protein LSm8

OrganismNot specified

UniProt P47093

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain 8； UniProt 1–109 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain b 1–109（109 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–109（109 aa）
Mutation：K17L,C22S,I38L,C51S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain H 1–109（109 aa）
Mutation：K17L,C22S,I38L,C51S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain A 1–96（96 aa）
Mutation：K17L,C22S,I38L,C51S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain H 1–96（96 aa）
Mutation：K17L,C22S,I38L,C51S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain H 1–96（96 aa）
Mutation：K17L,C22S,I38L,C51S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain A 1–96（96 aa）
Mutation：K17L,C22S,I38L,C51S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein–RNA Heteromer；Protein × 14 PDB declaration: hexadecameric
Chain A 1–96（96 aa）
Chain H 1–96（96 aa）
Mutation：K17L,C22S,I38L,C51S Mutation：K17L,C22S,I38L,C51S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain A 1–96（96 aa）
Mutation：K17L,C22S,I38L,C51S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain H 1–96（96 aa）
Mutation：K17L,C22S,I38L,C51S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain 8 1–109（109 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5VSU Structure of yeast U6 snRNP with 2'-phosphate terminated U6 RNA Deposited 2017-05-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain H 1–109（109 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；289 K；0.2 M NH4F 0.1 M HEPES pH 7.4 0.01 M MgCl2 18 % PEG 3,350
Resolution 3.10 Å R-free 0.298
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain z 1–109（109 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain z 1–109（109 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6ASO Structure of yeast U6 snRNP with 3'-phosphate terminated U6 RNA Deposited 2017-08-25 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain H 1–109（109 aa）
Not recorded K POTASSIUM ION × 2 MN MANGANESE (II) ION × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；280 mM sodium potassium tartrate 22.5 % glycerol 17.5 % PEG 3,350 1 mM manganese chloride
Resolution 2.71 Å R-free 0.247

10 other PDB entries and 15 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LSM8_YEAST
Isoform —
PDB entities 7
Chains and sequence ranges Author chain 8； PDBConstruct 1–109； UniProt 1–109

Pre-mRNA-splicing factor 8

OrganismNot specified

UniProt P33334

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain A； UniProt 1–2413 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2OG4 Structure of an expanded Jab1-MPN-like domain of splicing factor Prp8p from yeast Deposited 2007-01-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2147–2397（251 aa） Fragment：C-terminal domain (residues 2147-2397)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；2.5 % PEG 3350, 0.01 M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.241
3E66 Crystal structure of the beta-finger domain of yeast Prp8 Deposited 2008-08-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1822–2095（274 aa） Fragment：Beta-finger domain: UNP residues 1822-2095
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1M Tris-HCl pH 8.5, 10% PEG 8000, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.05 Å R-free 0.236
3E66 Crystal structure of the beta-finger domain of yeast Prp8 Deposited 2008-08-14 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1822–2095（274 aa） Fragment：Beta-finger domain: UNP residues 1822-2095
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0.1M Tris-HCl pH 8.5, 10% PEG 8000, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.05 Å R-free 0.236
3E9O Crystal Structure of Yeast Prp8, Residues 1836-2092 Deposited 2008-08-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1836–2087（252 aa） Fragment：RNA binding domain
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.00 Å R-free 0.245
3E9P Crystal Structure of Yeast Prp8, Residues 1827-2092 Deposited 2008-08-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1833–2087（255 aa） Fragment：RNA binding domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.10 Å R-free 0.298
3E9P Crystal Structure of Yeast Prp8, Residues 1827-2092 Deposited 2008-08-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1833–2087（255 aa） Fragment：RNA binding domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.10 Å R-free 0.298
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain A 1–2413（2413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
3SBG Crystal structure of a Prp8 C-terminal fragment Deposited 2011-06-04 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1836–2397（562 aa） Fragment：Yeast Prp8p C-terminal domain, 1836-2397
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；1 M ammonium tartrate, 5 mM NiCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.28 Å R-free 0.264
3SBT Crystal structure of a Aar2-Prp8 complex Deposited 2011-06-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2092（257 aa） Fragment：Yeast Prp8p RNaseH like domain, residues 1836-2092
Not recorded SO4 SULFATE ION × 2 PGO S-1,2-PROPANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；0.1 M Tris-HCl, pH 8.5, 0.1 M lithium sulfate, 20 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.80 Å R-free 0.197
3SBT Crystal structure of a Aar2-Prp8 complex Deposited 2011-06-06 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1836–2092（257 aa） Fragment：Yeast Prp8p RNaseH like domain, residues 1836-2092
Not recorded SO4 SULFATE ION × 2 PGO S-1,2-PROPANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；277 K；0.1 M Tris-HCl, pH 8.5, 0.1 M lithium sulfate, 20 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.80 Å R-free 0.197
3ZEF Crystal structure of Prp8:Aar2 complex: second crystal form at 3.1 Angstrom resolution Deposited 2012-12-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 885–2413（1529 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.9；7-9% PEG 8000, 100 MM NA-CITRATE PH 7.9, 50-200 MM AMMONIUM SULFATE
Resolution 3.10 Å R-free 0.267
3ZEF Crystal structure of Prp8:Aar2 complex: second crystal form at 3.1 Angstrom resolution Deposited 2012-12-05 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 885–2413（1529 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.9；7-9% PEG 8000, 100 MM NA-CITRATE PH 7.9, 50-200 MM AMMONIUM SULFATE
Resolution 3.10 Å R-free 0.267
4BGD Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8 Deposited 2013-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 2148–2395（248 aa） Fragment：RESIDUES 2148-2395
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 PE5 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；100 MM HEPESNA PH 7.5, 200 MM MGCL2, 32-40% PEG400
Resolution 3.10 Å R-free 0.270
4I43 Crystal structure of Prp8:Aar2 complex Deposited 2012-11-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 885–2413（1529 aa） Fragment：UNP residues 885-2413
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.9；293 K；7-9% PEG8000 0.1M sodium citrate 0.05-0.15M ammonium sulfate , pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 2.00 Å R-free 0.249
4ILG Crystal structure of Aar2p in complex with the Prp8p RNaseH and Jab1/MPN domains Deposited 2012-12-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2090)
Chain C 2147–2413（267 aa） Fragment：yPrp8 Jab1/MPN (UNP Residues 2147-2413)
Not recorded EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.1 M HEPES-NaOH, pH 7.5, 12 % (w/v) PEG 6000 and 0.6 M KCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 2.10 Å R-free 0.228
4ILH Crystal structure of an Aar2p C-terminal deletion mutant in conplex with Prp8p RNaseH Deposited 2012-12-31 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；277 K；0.1 M Tris-HCl, pH 8.0, 19 % (w/v) PEG 4000 and 100 mM KCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K
Resolution 1.85 Å R-free 0.217
4ILJ Crystal structure of an Prp8p RNaseH W1911A mutant protein Deposited 2012-12-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2090)
Mutation：W1911A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1 M Tris-Cl pH 8.5, 12% PEG 3350, 100 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.00 Å R-free 0.226
4ILJ Crystal structure of an Prp8p RNaseH W1911A mutant protein Deposited 2012-12-31 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2090)
Mutation：W1911A No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；298 K；0.1 M Tris-Cl pH 8.5, 12% PEG 3350, 100 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K
Resolution 2.00 Å R-free 0.226
5DCA Crystal structure of yeast full length Brr2 in complex with Prp8 Jab1 domain Deposited 2015-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain J 2148–2398（251 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M MES-NaOH, pH 6.5, 9 % (w/v) PEG 3350, 0.2 M MgCl2
Resolution 2.80 Å R-free 0.255
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric
Chain A 1–735（735 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain A 1–2413（2413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric
Chain A 2147–2413（267 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GAP Body region of the U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Dodecameric
Chain A 1–2413（2413 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted at 4 deg C for 2 seconds before plunging.
Resolution 3.60 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain A 128–2413（2286 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain A 1–2413（2413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain A 1–2413（2413 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain A 1–2413（2413 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain A 1–2413（2413 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5M52 Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain Deposited 2016-10-20 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 2147–2413（267 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1 M Tris-HCl pH 7.5, 10.5 % (w/v) PEG 3350, 0.2 M MgCl
Resolution 3.40 Å R-free 0.296
5M52 Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain Deposited 2016-10-20 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 2147–2413（267 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1 M Tris-HCl pH 7.5, 10.5 % (w/v) PEG 3350, 0.2 M MgCl
Resolution 3.40 Å R-free 0.296
5M5P S. cerevisiae spliceosomal helicase Brr2 (271-end) in complex with the Jab/MPN domain of S. cerevisiae Prp8 Deposited 2016-10-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 2147–2413（267 aa） Fragment：UNP residues 2147-2413
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；0.1 M MES pH 6.5 9.2% (w/v) PEG 4000 0.4 M MgCl2
Resolution 4.20 Å R-free 0.335
5M5P S. cerevisiae spliceosomal helicase Brr2 (271-end) in complex with the Jab/MPN domain of S. cerevisiae Prp8 Deposited 2016-10-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 2147–2413（267 aa） Fragment：UNP residues 2147-2413
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；0.1 M MES pH 6.5 9.2% (w/v) PEG 4000 0.4 M MgCl2
Resolution 4.20 Å R-free 0.335
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric
Chain A 1–2413（2413 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain A 1–2413（2413 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5QY1 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry A07a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded PGR R-1,2-PROPANEDIOL × 1 SO4 SULFATE ION × 2 R8A (2-aminopyridin-3-yl)methanol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.72 Å R-free 0.225
5QY2 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry A12b Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SO4 SULFATE ION × 4 PGR R-1,2-PROPANEDIOL × 3 TJ1 3-(propan-2-yl)-1,2,4-oxadiazol-5(4H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.36 Å R-free 0.218
5QY3 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry B03a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 2 SYG 2-[(1~{S})-1-azanylpropyl]phenol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.58 Å R-free 0.210
5QY4 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry B08a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded T9S ethyl 1,3-dihydro-2H-pyrrolo[3,4-c]pyridine-2-carboxylate × 2 SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.57 Å R-free 0.221
5QY5 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry C02a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SO4 SULFATE ION × 4 PGR R-1,2-PROPANEDIOL × 3 SYA 2,4,5-tris(fluoranyl)-3-methoxy-benzoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.211
5QY6 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry C05a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SY7 2-methoxy-4-[[(4~{S},5~{S})-2,4,5-tris(2-methoxypyridin-4-yl)imidazolidin-1-yl]methyl]pyridine × 1 SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.220
5QY7 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry C08a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 1 USJ (1S,3S)-2-methyl-2,3-dihydro-1H-isoindole-1,3-diol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.67 Å R-free 0.219
5QY8 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry C10a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded TJV 1,3-benzodioxole-5-carbothioamide × 1 SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.59 Å R-free 0.219
5QY9 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry C11b Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded R8Y N-ethyl-N-(thiophene-2-carbonyl)-beta-alanine × 1 SO4 SULFATE ION × 4 PGR R-1,2-PROPANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.40 Å R-free 0.210
5QYA PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry D02a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SY4 ~{N}-[5-azanyl-2,4-bis(fluoranyl)phenyl]propane-1-sulfonamide × 3 SO4 SULFATE ION × 3 PGR R-1,2-PROPANEDIOL × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.55 Å R-free 0.219
5QYB PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry D06a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded R9G (3-methoxyphenyl)(pyrrolidin-1-yl)methanone × 1 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 2.10 Å R-free 0.223
5QYC PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry D07a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SYJ 2-fluoranyl-~{N}-(furan-2-ylmethyl)benzenesulfonamide × 1 SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.70 Å R-free 0.210
5QYD PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry E05a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded R9S 2-(2-methylphenyl)acetamide × 1 PGR R-1,2-PROPANEDIOL × 1 SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.67 Å R-free 0.221
5QYE PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry E12a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded USP (2R)-6-amino-3-methyl-2,3-dihydro-1,3-benzoxazol-2-ol × 1 SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.217
5QYF PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry F02a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SO4 SULFATE ION × 4 PGR R-1,2-PROPANEDIOL × 3 T9Y ethyl 5-(trifluoromethyl)-1H-pyrazole-4-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.49 Å R-free 0.223
5QYG PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry F08a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 1 RBJ 4-[(methylamino)methyl]phenol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.67 Å R-free 0.219
5QYH PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry G04a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded T9V N-(4-methoxyphenyl)acetamide × 1 SO4 SULFATE ION × 3 PGR R-1,2-PROPANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.47 Å R-free 0.229
5QYI PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry G12a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 3 SZ7 ~{N}-[(~{E})-thiophen-2-ylmethylideneamino]cyclopropanecarboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.58 Å R-free 0.220
5QYJ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry H11a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded TBJ N-cyclopentyl-N'-{[(2R)-oxolan-2-yl]methyl}urea × 1 SO4 SULFATE ION × 2 PGR R-1,2-PROPANEDIOL × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.209
5QYK PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry H12a Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded SO4 SULFATE ION × 3 PGR R-1,2-PROPANEDIOL × 3 SZA ~{N}-(2-thiophen-2-ylethyl)pyridine-4-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.63 Å R-free 0.214
5QYL PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 01 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.56 Å R-free 0.225
5QYM PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 02 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.47 Å R-free 0.225
5QYN PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 03 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.65 Å R-free 0.218
5QYO PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 04 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.58 Å R-free 0.223
5QYP PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 05 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.61 Å R-free 0.223
5QYQ PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 06 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.67 Å R-free 0.242
5QYR PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 07 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.54 Å R-free 0.223
5QYS PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 08 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.52 Å R-free 0.222
5QYT PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 09 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.52 Å R-free 0.242
5QYU PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 10 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.227
5QYV PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 11 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.59 Å R-free 0.245
5QYW PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 12 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.63 Å R-free 0.219
5QYX PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 13 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.60 Å R-free 0.247
5QYY PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 14 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.71 Å R-free 0.228
5QYZ PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 15 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.37 Å R-free 0.224
5QZ0 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 16 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.57 Å R-free 0.245
5QZ1 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 17 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.58 Å R-free 0.247
5QZ2 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 17 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.54 Å R-free 0.222
5QZ3 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 18 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.53 Å R-free 0.284
5QZ4 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 19 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.75 Å R-free 0.236
5QZ5 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 20 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.218
5QZ6 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 21 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.32 Å R-free 0.224
5QZ7 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 22 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.48 Å R-free 0.214
5QZ8 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 23 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.62 Å R-free 0.252
5QZ9 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 24 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.43 Å R-free 0.220
5QZA PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 25 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.219
5QZB PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 26 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.69 Å R-free 0.244
5QZC PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 27 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.72 Å R-free 0.230
5QZD PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 28 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.59 Å R-free 0.263
5QZE PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 29 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.55 Å R-free 0.219
5QZF PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 30 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.68 Å R-free 0.234
5QZG PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 31 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.55 Å R-free 0.225
5QZH PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 32 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.77 Å R-free 0.221
5QZI PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 33 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.224
5QZJ PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 34 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.223
5QZK PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 35 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.73 Å R-free 0.269
5QZL PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 36 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.54 Å R-free 0.224
5QZM PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 37 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.66 Å R-free 0.218
5QZN PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 38 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.44 Å R-free 0.214
5QZO PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 39 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.39 Å R-free 0.221
5QZP PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 40 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.50 Å R-free 0.222
5QZQ PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 41 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 2.11 Å R-free 0.218
5QZR PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 42 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.59 Å R-free 0.223
5QZS PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 43 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.58 Å R-free 0.217
5QZT PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 44 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.53 Å R-free 0.238
5QZU PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 45 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.54 Å R-free 0.234
5QZV PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 46 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 2.11 Å R-free 0.205
5QZW PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 47 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.36 Å R-free 0.227
5QZX PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 48 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.55 Å R-free 0.221
5QZY PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 49 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.66 Å R-free 0.241
5QZZ PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 50 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.59 Å R-free 0.249
5R00 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 51 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.72 Å R-free 0.227
5R01 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 52 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.72 Å R-free 0.228
5R02 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 53 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.66 Å R-free 0.212
5R03 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 54 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.51 Å R-free 0.265
5R04 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 55 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.34 Å R-free 0.233
5R05 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 56 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.57 Å R-free 0.230
5R06 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 57 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.67 Å R-free 0.222
5R07 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 58 Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1835-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% (w/v) Peg 4000, 3% (v/v) DMSO, 0.1M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.71 Å R-free 0.222
5R08 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry A07, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded R8A (2-aminopyridin-3-yl)methanol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.70 Å R-free 0.262
5R09 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry B03, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded SYG 2-[(1~{S})-1-azanylpropyl]phenol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.56 Å R-free 0.242
5R0A PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry C02, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded SYA 2,4,5-tris(fluoranyl)-3-methoxy-benzoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.54 Å R-free 0.255
5R0B PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry C05, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded SY7 2-methoxy-4-[[(4~{S},5~{S})-2,4,5-tris(2-methoxypyridin-4-yl)imidazolidin-1-yl]methyl]pyridine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.82 Å R-free 0.264
5R0C PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry C08, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded R8V (3S)-3-hydroxy-2-methyl-2,3-dihydro-1H-isoindol-1-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.60 Å R-free 0.250
5R0D PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry C11, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded R8Y N-ethyl-N-(thiophene-2-carbonyl)-beta-alanine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.27 Å R-free 0.197
5R0E PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry D02, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded SY4 ~{N}-[5-azanyl-2,4-bis(fluoranyl)phenyl]propane-1-sulfonamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.59 Å R-free 0.236
5R0F PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry D06, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded R9G (3-methoxyphenyl)(pyrrolidin-1-yl)methanone × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.97 Å R-free 0.304
5R0G PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry D07, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded SYJ 2-fluoranyl-~{N}-(furan-2-ylmethyl)benzenesulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.73 Å R-free 0.340
5R0H PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry E05, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded R9S 2-(2-methylphenyl)acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.57 Å R-free 0.246
5R0I PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry E12, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded SYV 6-azanyl-3-methyl-1,3-benzoxazol-2-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.86 Å R-free 0.295
5R0J PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry F08, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded RBJ 4-[(methylamino)methyl]phenol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.81 Å R-free 0.281
5R0K PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry G12, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded SZ7 ~{N}-[(~{E})-thiophen-2-ylmethylideneamino]cyclopropanecarboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.80 Å R-free 0.302
5R0L PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry H11, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded TBJ N-cyclopentyl-N'-{[(2R)-oxolan-2-yl]methyl}urea × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.70 Å R-free 0.234
5R0M PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment F2X-Entry H12, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded SZA ~{N}-(2-thiophen-2-ylethyl)pyridine-4-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；20% (w/v) PEG 4000, 0.2M Tris-HCl pH 8.5, 0.2M Li2SO4
Resolution 1.70 Å R-free 0.251
5R0N PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 01, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.78 Å R-free 0.268
5R0O PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 02, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.86 Å R-free 0.264
5R0P PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 03, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.73 Å R-free 0.256
5R0Q PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 04, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.91 Å R-free 0.284
5R0R PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 05, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.73 Å R-free 0.260
5R0S PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 06, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.81 Å R-free 0.306
5R0T PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 07, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.96 Å R-free 0.262
5R0U PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 08, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.86 Å R-free 0.301
5R0V PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 09, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.81 Å R-free 0.320
5R0W PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 10, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.86 Å R-free 0.295
5R0X PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 11, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.84 Å R-free 0.273
5R0Y PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 12, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.75 Å R-free 0.342
5R0Z PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 13, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.86 Å R-free 0.283
5R10 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 14, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.70 Å R-free 0.255
5R11 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 15, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.82 Å R-free 0.264
5R12 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 16, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.70 Å R-free 0.298
5R13 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 18, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.87 Å R-free 0.275
5R14 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 19, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.74 Å R-free 0.253
5R15 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 20, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.79 Å R-free 0.252
5R16 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 21, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.84 Å R-free 0.252
5R17 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 22, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.87 Å R-free 0.296
5R18 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 23, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.79 Å R-free 0.257
5R19 PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 24, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.70 Å R-free 0.239
5R1A PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 25, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.73 Å R-free 0.252
5R1B PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 26, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.89 Å R-free 0.298
5R1C PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 27, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.91 Å R-free 0.281
5R1D PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 28, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.82 Å R-free 0.269
5R1E PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 29, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.70 Å R-free 0.239
5R1F PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 30, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.80 Å R-free 0.291
5R1G PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 31, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.81 Å R-free 0.303
5R1H PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 32, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 2.06 Å R-free 0.320
5R1I PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 33, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 2.01 Å R-free 0.330
5R1J PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 34, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.96 Å R-free 0.278
5R1K PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 35, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.99 Å R-free 0.326
5R1L PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 36, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.94 Å R-free 0.309
5R1M PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 37, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.90 Å R-free 0.299
5R1N PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 38, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.94 Å R-free 0.330
5R1O PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 39, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.90 Å R-free 0.334
5R1P PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 40, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.95 Å R-free 0.285
5R1Q PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 41, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 1.86 Å R-free 0.266
5R1S PanDDA analysis group deposition -- Auto-refined data of Aar2/RNaseH for ground state model 42, DMSO-free Deposited 2020-02-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：yPrp8 RNaseH (UNP Residues 1836-2096)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M ammonium acetate, 0.1 M sodium acetate, 24-30% PEG 4000
Resolution 2.05 Å R-free 0.307
5ST0 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02B04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UV9 1-amino-4,4-dimethylcyclohexane-1-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.241
5ST1 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02C03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UVO 5-methoxy-2-nitrosophenol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.235
5ST2 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02C06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UW6 5-methylpyridazin-3(2H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.53 Å R-free 0.245
5ST3 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02C09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UWI oxane-4-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.47 Å R-free 0.231
5ST4 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02D05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UWR (2R)-amino(3-chlorophenyl)acetonitrile × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.44 Å R-free 0.230
5ST5 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02D06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UXB (3S)-3-amino-3-(3-chlorophenyl)propan-1-ol × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.61 Å R-free 0.333
5ST6 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02D07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V0X (3R)-2,3-dihydro-1-benzofuran-3-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.62 Å R-free 0.245
5ST7 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02E03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V29 (3R)-3-(benzylamino)oxolane-3-carboxylic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.46 Å R-free 0.232
5ST8 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02E09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V33 (3-methoxyphenyl)acetic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.248
5ST9 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02F01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V8E (4-butanoylpiperazin-1-yl)acetic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.40 Å R-free 0.223
5STA PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02F03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V8T (1R)-1-{4-[(propan-2-yl)oxy]phenyl}ethan-1-amine × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.241
5STB PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02F05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V99 3-ethyl-5-(ethylsulfanyl)-4H-1,2,4-triazol-4-amine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.247
5STC PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02F09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V9P 1-(2-chloroethyl)-1H-imidazole × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.44 Å R-free 0.229
5STE PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02F11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VAV [2-methyl-5-(trifluoromethyl)pyrazol-3-yl]-oxidanyl-oxidanylidene-boron × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.237
5STF PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02H02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VOK 5,5-diethyl-1-methyl-1,3-diazinane-2,4,6-trione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.45 Å R-free 0.236
5STG PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02H08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VOW (4-ethoxyphenyl)-oxidanyl-oxidanylidene-boron × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.61 Å R-free 0.248
5STH PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02H09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded 8K2 5-chloranylthiophene-2-sulfonamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.293
5STI PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02H11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W8F 1-(3-methoxy-4-methylphenyl)methanamine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.76 Å R-free 0.260
5STJ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02H11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W8F 1-(3-methoxy-4-methylphenyl)methanamine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.76 Å R-free 0.260
5STK PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P02H12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W93 (2R)-2-amino-2-(3-methoxyphenyl)ethan-1-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.80 Å R-free 0.320
5STL PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03A02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W9E N-hydroxy-4-(trifluoromethyl)benzene-1-carboximidamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.245
5STM PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03A03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W9I 5-bromo-2-hydrazinylpyridine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.65 Å R-free 0.307
5STN PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03A11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W9O N-methyl-1-{(1S,2R)-2-[(1,2,4-triazolidin-1-yl)methyl]cyclohexyl}methanamine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.56 Å R-free 0.243
5STO PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03A12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W9U cyclohexyl[4-(hydroxyamino)piperidin-1-yl]methanone × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.231
5STP PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03B02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WA0 (1R)-2-(1H-imidazol-2-yl)-1-phenylethan-1-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.233
5STQ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03B03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VP0 [(1S)-2-acetyl-1,2,3,4-tetrahydroisoquinolin-1-yl]acetic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.50 Å R-free 0.253
5STR PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03B04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VNU N-(1-phenylcyclobutyl)glycinamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.45 Å R-free 0.221
5STS PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03B11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V0O ethyl 2-(cyclopropylamino)-1,3-thiazole-4-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.247
5STT PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03C03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V1U 2-(2-methoxyethoxy)benzoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.49 Å R-free 0.227
5STU PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03C07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V2U 2-chloro-N-(3,5-dimethoxyphenyl)acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.242
5STV PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03C11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V86 2-bromo-N-ethylbenzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.50 Å R-free 0.242
5STW PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03C12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V8L N'-phenylacetohydrazide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.89 Å R-free 0.341
5STX PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03D01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V92 4-hydroxy-2-oxo-1,2-dihydroquinoline-3-carbonitrile × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.50 Å R-free 0.240
5STY PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03D02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V9I ethyl (3S)-3-(methoxycarbamoyl)piperidine-1-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.235
5STZ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03D04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V9X 3-(1H-indol-3-yl)propanamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.44 Å R-free 0.222
5SU0 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03D07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VOE (2R)-2-phenylbutan-1-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.241
5SU1 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03D08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VOQ N-[(3,5-dimethyl-1,2-oxazol-4-yl)methyl]-N,2-dimethyl-L-alaninamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.229
5SU2 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03D10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W7X 1-(6-methylpyridin-2-yl)-1,4-diazepane × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.85 Å R-free 0.316
5SU3 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03D12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W8B 4-chloro-5-(morpholin-4-yl)pyridazin-3(2H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.65 Å R-free 0.249
5SU4 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03E02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W8K N-[(3-bromophenyl)methyl]acetamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.66 Å R-free 0.357
5SU5 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03E05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W8W 5-bromo-1-methylpyrimidine-2,4(1H,3H)-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.81 Å R-free 0.299
5SU6 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03E08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W98 N-cyclopropyl-N-[(thiophen-2-yl)methyl]methanesulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.237
5SU7 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03E12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V3R (2S)-4-(3,4-difluorophenyl)butan-2-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.81 Å R-free 0.346
5SU8 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03F01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V40 (2S)-1-{[(1R,2R)-2-(aminomethyl)cyclohexyl]methyl}pyrrolidin-2-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.79 Å R-free 0.255
5SU9 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03F06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V4L N~2~-methyl-N~2~-[2-methyl-2-(4-methylphenyl)propyl]glycinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.66 Å R-free 0.255
5SUA PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03F12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V4X 3-oxo-3-(thiomorpholin-4-yl)propanenitrile × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.53 Å R-free 0.233
5SUB PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03G01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V6R (2S)-2-amino-N-[(2-fluorophenyl)methyl]-N-methylbutanamide × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.235
5SUC PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03G02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V73 N-(2-hydroxyethyl)-N-methyl-2-phenylacetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.225
5SUD PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03G05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V7C N-[(2R)-2-cyanopropyl]benzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.48 Å R-free 0.241
5SUE PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03G10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WA8 2-(2-oxopyridin-1(2H)-yl)-N,N-di(prop-2-en-1-yl)acetamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.70 Å R-free 0.259
5SUF PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03H03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded SO4 SULFATE ION × 1 W3U 1-benzyl-1H-pyrazole-4-carboxylic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.254
5SUG PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03H05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W1E 2-methylpropyl [(3R)-1,1-dioxo-2,3-dihydro-1H-1lambda~6~-thiophen-3-yl]acetate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.53 Å R-free 0.236
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain A 1–2413（2413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain A 1–2413（2413 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain A 1–2413（2413 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain A 1–2413（2413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain A 1–2413（2413 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain A 1–2413（2413 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain A 1–2413（2413 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain A 1–2413（2413 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain A 1–2413（2413 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain A 1–2413（2413 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain A 1–2413（2413 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6TEO Crystal structure of a yeast Snu114-Prp8 complex Deposited 2019-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 315–555（241 aa）
Not recorded SO4 SULFATE ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；10% (w/v) PEG8000, 200mM Lithium chloride, 50mM magnesium sulfate
Resolution 3.10 Å R-free 0.271
6TEO Crystal structure of a yeast Snu114-Prp8 complex Deposited 2019-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 315–555（241 aa）
Not recorded SO4 SULFATE ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；10% (w/v) PEG8000, 200mM Lithium chloride, 50mM magnesium sulfate
Resolution 3.10 Å R-free 0.271
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric
Chain A 1–2413（2413 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7FJU PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03H10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W1N (3S)-3-amino-4-(2-fluorophenyl)butanoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.63 Å R-free 0.240
7FJV PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03H10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W1N (3S)-3-amino-4-(2-fluorophenyl)butanoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.63 Å R-free 0.240
7FJW PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P03H12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W1W 6-[(pyridin-2-yl)sulfanyl]pyridin-3-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.63 Å R-free 0.308
7FJX PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04A01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W2B N-cyclohexyl-2-(1H-pyrazol-1-yl)acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.242
7FJY PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04A02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W2I 1-[(3-aminophenyl)methyl]piperidin-4-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.63 Å R-free 0.246
7FJZ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04A04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W2O D-phenylalaninamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.253
7FK0 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04A05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W36 2-amino-N-(3-fluoro-4-methylphenyl)ethane-1-sulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.69 Å R-free 0.331
7FK1 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04A06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W3H (3R)-3-(4-chlorobenzene-1-sulfonyl)butanoic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.76 Å R-free 0.315
7FK2 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04A07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VF3 4-(thiophene-2-sulfonyl)morpholine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.35 Å R-free 0.245
7FK3 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04A09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VNC methyl N-(benzenesulfonyl)-N-methylglycinate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.235
7FK4 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04A11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VNN (4-methylpiperazin-1-yl)(5-methylthiophen-3-yl)methanone × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.234
7FK5 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04A12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V3F (5S)-5-methyl-5-phenylimidazolidine-2,4-dione × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.53 Å R-free 0.227
7FK6 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04B02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WE5 methyl (3R)-5-oxo-1-phenylpyrrolidine-3-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.61 Å R-free 0.262
7FK7 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04B04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V4C N-cyclopropyl-1-methyl-N-(2-methylpropyl)-1H-pyrazole-4-sulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.246
7FK8 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04B07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WAO 2-[(3-chloro-4-fluorophenyl)methyl]-4-methyl-2,4-dihydro-3H-1,2,4-triazol-3-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.303
7FK9 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04B12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WKL N-(2-aminoethyl)-N-(cyanomethyl)thiophene-2-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.35 Å R-free 0.239
7FKA PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04C04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V6Z methyl N-(2-chlorobenzoyl)glycinate × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.234
7FKB PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04C07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W3Q N-[(2-methoxyphenyl)methyl]urea × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.68 Å R-free 0.317
7FKC PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04C10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V7L N,N~2~-dimethyl-N-(pyridin-2-yl)glycinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.250
7FKD PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04C11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WAE N-(2,6-dimethylphenyl)methanesulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.45 Å R-free 0.232
7FKE PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04C12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WAH N-ethyl-1-(4-fluorophenyl)methanesulfonamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.48 Å R-free 0.236
7FKF PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04D04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W1I N'-(2-bromophenyl)-N,N-dimethylsulfuric diamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.69 Å R-free 0.248
7FKG PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04D05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WOR N'-(2-hydroxy-5-methylphenyl)-N-methyl-N-(propan-2-yl)sulfuric diamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.241
7FKH PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04D06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WAX (3-aminophenyl)(morpholin-4-yl)methanone × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.44 Å R-free 0.235
7FKI PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04D08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W25 (2R)-(3,4-difluorophenyl)(hydroxy)acetic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.47 Å R-free 0.236
7FKJ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04D11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WB6 5-chloro-N,1-dimethyl-N-[(2S)-pentan-2-yl]-1H-imidazole-4-sulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.74 Å R-free 0.264
7FKK PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04D12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WBB N-methoxy-2-(2-methylphenyl)acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.63 Å R-free 0.292
7FKL PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04E02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W2U (2S)-2-phenoxypropanehydrazide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.63 Å R-free 0.292
7FKM PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04E03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W3B methyl N-[3-chloro-4-(difluoromethoxy)phenyl]glycinate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.53 Å R-free 0.239
7FKN PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04E05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded AC6 P-HYDROXYACETOPHENONE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.251
7FKO PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04E07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WBF 2-[(2-fluorophenyl)sulfanyl]acetohydrazide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.234
7FKP PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04E08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WBL propyl (2S)-2-[(5-amino-1,3,4-thiadiazol-2-yl)sulfanyl]propanoate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.52 Å R-free 0.231
7FKQ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04E09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VRY 1-[(2-chloro-4-methoxyphenyl)methyl]-1H-1,2,4-triazole × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.68 Å R-free 0.248
7FKR PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04E11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VQ6 (2R)-(cyclopropylamino)(2,6-difluorophenyl)acetic acid × 1 SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.69 Å R-free 0.242
7FKS PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04F01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VQB 4-chloro-6-(pyrrolidin-1-yl)pyrimidin-5-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.263
7FKT PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04F02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VBI N~2~-(3-chlorophenyl)-N~2~-(methanesulfonyl)-N-methylglycinamide × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.44 Å R-free 0.247
7FKU PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04F03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VBX N-methyl-2-{[2-(methylamino)-2-oxoethyl]amino}benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.247
7FKV PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04F08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VCO ethyl (2Z)-(azepan-2-ylidene)(cyano)acetate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.75 Å R-free 0.289
7FKW PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04F09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VD6 (2R)-1-benzylpiperidine-2-carboxylic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.47 Å R-free 0.225
7FKX PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04G02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VDI 1-(4-chlorophenyl)cyclobutane-1-carboxylic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.85 Å R-free 0.275
7FKY PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04G05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VKO 1-{[(2-methylphenyl)methyl]carbamoyl}cyclopropane-1-carboxylic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.274
7FKZ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04G08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VL8 (2R)-2-(3,4-dichlorophenoxy)-N-methylbutanamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.254
7FL0 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04G11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VNX 5-cyclopropyl-2H-tetrazole × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.236
7FL1 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04H03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VO9 N-cyclopropyl-N-(2-hydroxyethyl)thiophene-2-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.231
7FL2 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04H05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UYR (2E)-2-cyano-3-(thiophen-3-yl)prop-2-enamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.252
7FL3 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04H06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UZ8 [2-(methylsulfanyl)pyridin-3-yl](pyrrolidin-1-yl)methanone × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.239
7FL4 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04H06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UZ8 [2-(methylsulfanyl)pyridin-3-yl](pyrrolidin-1-yl)methanone × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.239
7FL5 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04H07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V0C (2S)-(4-bromophenyl)(piperazin-1-yl)acetonitrile × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.89 Å R-free 0.283
7FL6 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P04H10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VIK methyl [(3S)-1,1-dioxo-1lambda~6~-thiolan-3-yl]acetate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.231
7FL7 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05A01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VIX (2R)-(4-chlorophenyl)(morpholin-4-yl)acetic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.229
7FL8 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05A03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VJ9 ethyl 4-fluoro-3-nitrobenzoate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.242
7FL9 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05A08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VJO N-(2,3-dihydro-1,4-benzodioxin-6-yl)methanesulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.236
7FLA PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05A09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VJY N-[(5-acetylthiophen-2-yl)methyl]acetamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.87 Å R-free 0.254
7FLB PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05A10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VKF 4-(difluoromethyl)benzoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.254
7FLC PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05B08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VPW 4-(4-chlorophenyl)-1,2,3,6-tetrahydropyridine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.95 Å R-free 0.318
7FLD PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05B11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VQ2 [4-(diethylcarbamoyl)phenyl]boronic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.242
7FLE PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05C05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VB9 3-[2-(dimethylamino)ethyl]-2-sulfanylidene-2,3-dihydrothieno[3,2-d]pyrimidin-4(1H)-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.240
7FLF PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05C06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VBO methyl 3-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)propanoate × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.248
7FLG PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05D01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded L5W ~{N}-oxidanylbicyclo[2.2.1]heptane-1-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.251
7FLH PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05D03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VCX methyl 4-bromo-5-methyl-1H-pyrazole-3-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.248
7FLI PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05D09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VDD (2S)-2-[(pyridin-2-yl)sulfanyl]propanoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.75 Å R-free 0.269
7FLJ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05E05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VE0 N-hydroxy-2-(piperidin-1-yl)pyridine-3-carboximidamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.47 Å R-free 0.230
7FLK PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05E08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VKX (5R,8aS)-8a-phenylhexahydropyrrolo[1,2-a]pyrimidin-6(2H)-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.62 Å R-free 0.239
7FLL PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05E12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VLI (3S)-3-hydroxy-6-methyl-3-(trifluoromethyl)-1,3-dihydro-2H-indol-2-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.48 Å R-free 0.236
7FLM PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05F01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VO5 N-ethyl-2-methoxybenzene-1-sulfonamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.232
7FLN PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05F09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UYL (5R)-2,7-diazaspiro[4.5]dec-1-en-3-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.234
7FLO PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05F11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UYY 2-methoxybenzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.242
7FLP PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05F11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded UYY 2-methoxybenzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.242
7FLQ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05F12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V03 (3S)-3-amino-3-(4-methoxyphenyl)propan-1-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.73 Å R-free 0.275
7FLR PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05G01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VIE 2-(aminomethyl)-N,N-dimethyl-2,3-dihydro-1H-inden-2-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.63 Å R-free 0.268
7FLS PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05G02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VIP 3-[(2S)-2-amino-1-hydroxypropan-2-yl]phenol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.233
7FLT PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05G04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VJ0 (1S)-1-(3-fluoro-4-methoxyphenyl)ethan-1-amine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.73 Å R-free 0.305
7FLU PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05G05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VJI (1R)-1-(2,3-dihydro-1,4-benzodioxin-6-yl)ethane-1,2-diamine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.223
7FLV PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05G05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VJI (1R)-1-(2,3-dihydro-1,4-benzodioxin-6-yl)ethane-1,2-diamine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.223
7FLW PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05G07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V5I 2-methyl-1-(2-methylphenyl)propan-2-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.255
7FLX PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05G08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded 5G1 2-chlorobenzohydrazide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.75 Å R-free 0.287
7FLY PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05G10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V5Y piperidine-4-carboxamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.243
7FLZ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05G11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V6I N-(2-fluorophenyl)methanesulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.52 Å R-free 0.258
7FM0 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05H01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VRC (4S)-2-(aminomethyl)-4-nitrocyclohexa-2,5-dien-1-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.61 Å R-free 0.246
7FM1 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05H02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VRL methyl (1R)-6-hydroxy-1,2,3,4-tetrahydroisoquinoline-1-carboxylate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.50 Å R-free 0.233
7FM2 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05H04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VS7 (2R)-1-(1H-indol-3-yl)propan-2-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.319
7FM3 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P05H09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VSL methyl 4,5,6,7-tetrahydro-2H-indazole-3-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.65 Å R-free 0.317
7FM4 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06A01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VST (4Z)-6-fluoro-4-(methoxyimino)-3,4-dihydro-1-benzothiopyran-1,1(2H)-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.62 Å R-free 0.253
7FM5 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06A02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VSX N-(2-amino-5-chlorophenyl)acetamide × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.56 Å R-free 0.234
7FM6 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06A03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VTH 4-methoxy-2-nitrobenzonitrile × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.250
7FM7 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06A04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VTL 4-(2-hydroxyethyl)-2-methoxyphenol × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.245
7FM8 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06A09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VQF (5S,6Z)-6-imino-1,3-dimethyl-5-propyl-1,3-diazinane-2,4-dione × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.242
7FM9 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06B02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VQQ 2-(5-chloro-2-methoxyphenyl)ethan-1-amine × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.259
7FMA PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06B04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VQZ N-[2-(aminomethyl)phenyl]methanesulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.41 Å R-free 0.230
7FMB PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06B06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VTT 3-methylthiophene-2-carbohydrazide × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.242
7FMC PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06B07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VU5 1-[(2S)-2-hydroxy-3-(methylamino)propyl]pyrrolidin-2-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.235
7FMD PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06B12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VUE 2-(4-chloro-2-nitroanilino)ethan-1-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.68 Å R-free 0.273
7FME PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06C01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VUI 4-bromo-1-(2-methoxyethoxy)-2-nitrobenzene × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.44 Å R-free 0.225
7FMF PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06C06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VUU N-cyclopropyl-N'-[2-(thiophen-2-yl)ethyl]guanidine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.65 Å R-free 0.244
7FMG PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06C09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VV5 N-[2-(thiophen-2-yl)ethyl]pyridine-3-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.71 Å R-free 0.322
7FMH PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06C10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V5C (2E)-N-(2-cyanoethyl)-N-methyl-3-(2-methyl-1,3-thiazol-4-yl)prop-2-enamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.254
7FMI PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06C11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WBQ 6-(2-methoxyanilino)-6-oxohexanoic acid × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.34 Å R-free 0.213
7FMJ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06C12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V5O (2E)-3-[4-(2-oxopyrrolidin-1-yl)phenyl]prop-2-enoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.50 Å R-free 0.253
7FMK PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06D03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VRR ethyl N-benzyl-beta-alaninate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.47 Å R-free 0.216
7FML PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06D04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VR6 N-[(furan-2-yl)methyl]-2-[(pyridin-2-yl)oxy]ethan-1-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.239
7FMM PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06D09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VRH N-methoxy-2-(2-methoxy-4-methylphenoxy)acetamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.69 Å R-free 0.287
7FMN PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06D11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VVF 2-cyano-N-[3-(trifluoromethyl)phenyl]acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.60 Å R-free 0.254
7FMO PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06D12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VVK N-(2-methoxyphenyl)-2-methyl-L-alanine × 1 DMS DIMETHYL SULFOXIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.245
7FMP PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06E01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VSQ N~2~-phenylglycinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.310
7FMQ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06E04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VVR 3-phenoxypropanoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.241
7FMR PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06E05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VTC 3-aminophenyl dimethylcarbamate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.246
7FMS PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06E05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VTC 3-aminophenyl dimethylcarbamate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.58 Å R-free 0.246
7FMT PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06E09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VTO ethyl 2-aminopyridine-4-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.246
7FMU PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06E11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VQL (6P)-4-(methylsulfanyl)-6-(2-methyl-1,3-thiazol-4-yl)-1,3,5-triazin-2-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.81 Å R-free 0.287
7FMV PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06E12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VQU (2R,4S)-2-(pyridin-3-yl)-1,3-thiazolidine-4-carboxylic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.61 Å R-free 0.257
7FMW PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06F06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VTW 3-phenyl-1,2-oxazole-5-carboxylic acid × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.249
7FMX PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06F06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VTW 3-phenyl-1,2-oxazole-5-carboxylic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.249
7FMY PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06F07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VU9 1-phenylpiperazine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.65 Å R-free 0.253
7FMZ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06F08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WBX methyl [4-(1,3-thiazol-2-yl)piperazin-1-yl]acetate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.271
7FN0 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06G05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VUN 4-{[(propan-2-yl)oxy]methyl}benzoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.240
7FN1 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06G06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VV0 4-amino-3-methyl-N-(propan-2-yl)benzamide × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.44 Å R-free 0.236
7FN2 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06G08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VXF 1-[2-(morpholin-4-yl)pyridin-4-yl]methanamine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.62 Å R-free 0.294
7FN3 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06G09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VP8 (3-ethoxyphenyl)boronic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.63 Å R-free 0.236
7FN4 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06G11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VPK ethyl pyridin-3-ylcarbamate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.330
7FN5 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06H04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VXK 5-bromo-N-propylthiophene-3-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.69 Å R-free 0.259
7FN6 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06H06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VY2 ethyl (3-methylanilino)(oxo)acetate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.50 Å R-free 0.232
7FN7 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P06H08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WC2 6-chloro-N-(3-methoxypropyl)pyrimidin-4-amine × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.232
7FN8 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07A01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VYK N~2~-[(3-chlorophenyl)methyl]-N~2~-methylglycinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.41 Å R-free 0.250
7FN9 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07A05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VYU 4-(4-ethylpiperazin-1-yl)aniline × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.243
7FNA PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07A11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VZ3 3-bromo-4-methoxy-N-methylbenzene-1-sulfonamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.69 Å R-free 0.330
7FNB PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07A12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VZC 2-cyano-N-methyl-N-phenylacetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.240
7FNC PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07B01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VZL N-{2-[(4R)-8-methylimidazo[1,2-a]pyridin-2-yl]ethyl}acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.258
7FND PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07B06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VZU ethyl (4-aminophenyl)methylcarbamate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.233
7FNE PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07B07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W0C N-(pentan-3-yl)-N'-(pyridin-3-yl)urea × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.246
7FNF PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07B10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W0N N-(2-methylpentan-2-yl)-1H-pyrazole-3-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.246
7FNG PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07B12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded LAZ N-(2-AMINOETHYL)-P-CHLOROBENZAMIDE × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.60 Å R-free 0.264
7FNH PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07C05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W18 4-amino-N-[(2R)-1-hydroxybutan-2-yl]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.247
7FNI PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07C09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VW8 (1E)-N-hydroxy(phenoxy)ethanimidamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.229
7FNJ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07C10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VWH 2-[(cyclopentylamino)methyl]-6-methoxyphenol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.249
7FNK PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07C12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded LX4 4-cyclopentylcarbonylpiperazin-2-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.252
7FNL PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07D01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VWR 1-ethyl-N-[(thiophen-2-yl)methyl]-1H-tetrazol-5-amine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.245
7FNM PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07D05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VXB 5-bromo-N-(2-methoxyethyl)pyridine-3-carboxamide × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.284
7FNN PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07D07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WC6 1-(3-fluorophenyl)-2-(1H-1,2,4-triazol-1-yl)ethan-1-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.65 Å R-free 0.268
7FNO PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07D12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VP3 4-(4-fluorophenyl)-4-oxobutanoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.65 Å R-free 0.248
7FNP PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07E01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VPE (3S)-3-methyl-5-oxo-5-[(1,3-thiazol-2-yl)amino]pentanoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.242
7FNQ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07E01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VPE (3S)-3-methyl-5-oxo-5-[(1,3-thiazol-2-yl)amino]pentanoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.242
7FNR PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07E02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VXW N-[(4-methylphenyl)methyl]-3-oxobutanamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.60 Å R-free 0.262
7FNS PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07E03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VY6 2-[(morpholin-4-yl)methyl]furan-3-carboxylic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.45 Å R-free 0.243
7FNT PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07E12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VYB 2-{[(3-bromophenyl)methyl]amino}ethan-1-ol × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.61 Å R-free 0.315
7FNU PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07F03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VYO methyl [(5-amino-1,3,4-thiadiazol-2-yl)sulfanyl]acetate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.232
7FNV PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07F04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VYZ N-(4-nitrophenyl)acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.234
7FNW PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07F07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VZ9 2-{4-[(4-fluorophenyl)methyl]piperazin-1-yl}ethan-1-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.90 Å R-free 0.288
7FNX PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07F08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VZH 4-methoxy-N-methylbenzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.259
7FNY PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07F10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VZQ 4-[(acetyloxy)methyl]benzoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.71 Å R-free 0.263
7FNZ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07F12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W00 2-[(2-amino-2-oxoethyl)sulfanyl]-N-(4-fluorophenyl)acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.66 Å R-free 0.268
7FO0 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07G02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W0H 2-(4-fluorophenoxy)-N-(2-methoxyethyl)acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.83 Å R-free 0.287
7FO1 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07G03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W0R 3-[(2-methylpropyl)sulfanyl]-N-(pyridin-3-yl)propanamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.50 Å R-free 0.226
7FO2 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07G04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W0X 3-[(3-bromophenyl)methanesulfonyl]propanoic acid × 2 DMS DIMETHYL SULFOXIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 2.01 Å R-free 0.294
7FO3 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07G06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VW3 N-hydroxy-3-phenoxypropanamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.246
7FO4 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07G08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VWC 4-(2-methylpropoxy)benzohydrazide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.53 Å R-free 0.242
7FO5 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07G11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VWL N-(4-aminophenyl)-5-methylfuran-3-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.71 Å R-free 0.289
7FO6 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07H04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WCB 1-[(2S)-2-methylmorpholin-4-yl]-2-(thiophen-3-yl)ethan-1-one × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.57 Å R-free 0.277
7FO7 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07H05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VX5 (3R)-N-cyclohexylpiperidine-3-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.45 Å R-free 0.234
7FO8 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07H06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VGU N-[(1E)-2-(hydroxyamino)-2-oxoethylidene]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.314
7FO9 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07H07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VHO N-cyclopropyl-2-(3-fluorophenoxy)acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.278
7FOA PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07H07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VHO N-cyclopropyl-2-(3-fluorophenoxy)acetamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.278
7FOB PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P07H11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VI5 N-(3-fluorophenyl)-N~2~-methylglycinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.257
7FOC PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08A01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W55 2H-1-benzopyran-3-carboxylic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.90 Å R-free 0.248
7FOD PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08A07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W5H (1-methyl-1H-pyrrol-2-yl)acetic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.249
7FOE PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08A08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WCI N-(3,4-dichlorophenyl)-2-(1H-pyrazol-1-yl)acetamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.65 Å R-free 0.296
7FOF PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08A10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W5L (2R)-1-amino-3-(4-chloro-3-methylphenoxy)propan-2-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.77 Å R-free 0.290
7FOG PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08B02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W5Q N~2~-[(1S)-1-(2,4-difluorophenyl)ethyl]-N-ethylglycinamide × 1 WKH N~2~-[(1R)-1-(2,4-difluorophenyl)ethyl]-N-ethylglycinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.41 Å R-free 0.240
7FOH PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08B05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W5U N-[2-(dimethylamino)ethyl]-4-fluorobenzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.69 Å R-free 0.336
7FOI PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08B06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W5Z 3-fluoro-N-[2-(1H-imidazol-1-yl)ethyl]benzamide × 4 PGR R-1,2-PROPANEDIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.249
7FOJ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08B08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W63 (2S)-N-(3-chlorophenyl)-2-[(2-hydroxyethyl)sulfanyl]propanamide × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.65 Å R-free 0.261
7FOK PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08B09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W6H methyl N-methyl-N-[(2E)-3-(thiophen-3-yl)prop-2-enoyl]glycinate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.72 Å R-free 0.297
7FOL PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08C01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W6R N-[(thiophen-2-yl)methyl]butanamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.254
7FOM PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08C04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W7T N-(2-aminoethyl)-2-cyclohexylacetamide × 3 DMS DIMETHYL SULFOXIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.235
7FON PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08C04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W7T N-(2-aminoethyl)-2-cyclohexylacetamide × 2 DMS DIMETHYL SULFOXIDE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.235
7FOO PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08C06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded DEW 2-azanyl-1-(6,7-dihydro-4~{H}-thieno[3,2-c]pyridin-5-yl)ethanone × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.279
7FOP PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08C08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W4H N-[(1S)-1-cyanopropyl]benzamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.66 Å R-free 0.304
7FOQ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08C10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W4R methyl N-[(2,6-dichlorophenyl)acetyl]-N-methylglycinate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.74 Å R-free 0.269
7FOR PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08C12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W50 (2S)-1-(2-bromophenoxy)-3-(pyrrolidin-1-yl)propan-2-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 2.03 Å R-free 0.378
7FOS PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08D01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VFL (2R)-1-amino-3-(2,4-dimethylphenoxy)propan-2-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.67 Å R-free 0.255
7FOT PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08D03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VFR (3E)-N-benzyl-3-(hydroxyamino)-3-iminopropanamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.56 Å R-free 0.241
7FOU PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08E01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VH9 (1R)-1-(4-fluorophenyl)-2-[(propan-2-yl)amino]ethan-1-ol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.239
7FOV PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08E03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WCT 3-methyl-N-[(3S)-4,4,4-trifluoro-3-hydroxybutyl]-1,2-oxazole-5-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.48 Å R-free 0.226
7FOW PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08E04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VI2 N-(2-hydroxyethyl)-N'-(3-methylphenyl)thiourea × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.51 Å R-free 0.214
7FOX PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08E08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VI8 3-{[(pyridin-3-yl)methyl]amino}benzoic acid × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.47 Å R-free 0.238
7FOY PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08E10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W5C 2-[(4-methyl-4H-1,2,4-triazol-3-yl)sulfanyl]-1-phenylethan-1-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.251
7FOZ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08F01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WD0 (3aS,6R)-N-(2-methoxyethyl)-2,3,3a,6-tetrahydro-1,3-benzothiazole-6-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.83 Å R-free 0.341
7FP0 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08F02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WD4 1-cyclopropylimidazolidin-2-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.54 Å R-free 0.235
7FP1 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08F04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WD8 methyl N-(propan-2-yl)-N-(thiophene-3-carbonyl)glycinate × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.43 Å R-free 0.222
7FP2 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08F10 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WDC 2-{[(5-amino-2-chlorophenyl)methyl](methyl)amino}ethan-1-ol × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.64 Å R-free 0.253
7FP3 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08F11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WDH N,N~2~-diethyl-N~2~-(2-methylpyrimidin-4-yl)glycinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.76 Å R-free 0.311
7FP4 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08G02 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WDN methyl N-methyl-N-(thiophene-3-carbonyl)glycinate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.45 Å R-free 0.222
7FP5 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08G04 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W6C N-[(2S)-1-methoxypropan-2-yl]-1-methyl-6-oxo-1,4,5,6-tetrahydropyridazine-3-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.53 Å R-free 0.236
7FP6 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08G05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W6L (3R)-3-amino-3-phenylpropanenitrile × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.61 Å R-free 0.229
7FP7 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08G09 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded WDZ methyl (5-cyclopentyl-1H-tetrazol-1-yl)acetate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.47 Å R-free 0.244
7FP8 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P08H12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W6W N~3~-[(2-bromophenyl)methyl]-N~3~-methyl-beta-alaninamide × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.247
7FP9 PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09A12 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W3Z ethyl (3S)-1-(methanesulfonyl)piperidine-3-carboxylate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.90 Å R-free 0.306
7FPA PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09C01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W4C 2,4-dichloro-N-ethyl-N-(2-hydroxyethyl)benzamide × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.86 Å R-free 0.289
7FPB PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09C03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W4L N~2~-[(6-chloropyridin-3-yl)methyl]-N,N~2~-diethylglycinamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 2.02 Å R-free 0.289
7FPC PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09D07 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded W4W ({2-[(2S)-2-ethylpiperidin-1-yl]-2-oxoethyl}sulfanyl)acetonitrile × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.264
7FPD PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09E01 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VF9 1-[(2S)-2-(furan-2-yl)azepan-1-yl]ethan-1-one × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.61 Å R-free 0.321
7FPE PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09E03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VE3 N,N,4-triethyl-1,2,3-thiadiazole-5-carboxamide × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.252
7FPF PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09E08 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VEC methyl N-(3-hydroxybenzoyl)-N-methylglycinate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.55 Å R-free 0.258
7FPG PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09F06 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VEL [4-(difluoromethoxy)phenyl]methanol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.60 Å R-free 0.265
7FPH PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P09G03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded VEX (2,3,4-trimethoxyphenyl)methanol × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.91 Å R-free 0.267
7FPI PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P10C11 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V1I N,N'-dicyclopropyl-N-(prop-2-en-1-yl)urea × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.63 Å R-free 0.259
7FPJ PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P10D05 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V2L 1-(1-benzyl-1H-imidazol-2-yl)methanamine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.261
7FPK PanDDA analysis group deposition -- Aar2/RNaseH in complex with fragment P10E03 from the F2X-Universal Library Deposited 2022-08-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1836–2090（255 aa） Fragment：UNP residues 1836-2090
Not recorded V7X 1-[2-(pyrrolidin-1-yl)pyridin-3-yl]methanamine × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；19% PEG4000, 3% DMSO, 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate
Resolution 1.59 Å R-free 0.270
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain A 1–2413（2413 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

435 other PDB entries and 443 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PRP8_YEAST
Isoform —
PDB entities 8
Chains and sequence ranges Author chain A； PDBConstruct 1–2413； UniProt 1–2413

Pre-mRNA-splicing helicase BRR2

OrganismNot specified

UniProt P32639

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain B； UniProt 1–2163 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3HIB Crystal structure of the second Sec63 domain of yeast Brr2 Deposited 2009-05-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1851–2163（313 aa） Fragment：Second Sec63 domain: UNP residues 1851-2163
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；0.1M Sodium citrate, 17% PEG 8000, 0.2M NaCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.00 Å R-free 0.257
3IM1 Structure of the C-terminal Sec63 unit of yeast Brr2, P212121 Form Deposited 2009-08-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1839–2163（325 aa） Fragment：Sec63 unit (UNP residues 1839-2163)
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；100mM sodium cacodylate, pH 6.0, 100mM Li2SO4, 15% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.65 Å R-free 0.232
3IM2 Structure of the C-terminal Sec63 unit of yeast Brr2, P41212 Form Deposited 2009-08-09 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1839–2163（325 aa） Fragment：Sec63 unit (UNP residues 1839-2163)
Not recorded PG4 TETRAETHYLENE GLYCOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；100mM sodium cacodylate, pH 6.0, 100mM Li2SO4, 15 % PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 1.99 Å R-free 0.237
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain N 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4BGD Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8 Deposited 2013-03-25 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 442–2163（1722 aa） Fragment：RESIDUES 442-2163
Not recorded ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 PE5 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.5；100 MM HEPESNA PH 7.5, 200 MM MGCL2, 32-40% PEG400
Resolution 3.10 Å R-free 0.270
5DCA Crystal structure of yeast full length Brr2 in complex with Prp8 Jab1 domain Deposited 2015-08-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 113–2163（2051 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M MES-NaOH, pH 6.5, 9 % (w/v) PEG 3350, 0.2 M MgCl2
Resolution 2.80 Å R-free 0.255
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain B 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric
Chain B 1–2163（2163 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GAP Body region of the U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Dodecameric
Chain B 1–2163（2163 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted at 4 deg C for 2 seconds before plunging.
Resolution 3.60 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain B 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain B 1–2163（2163 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain C 1–2163（2163 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5M52 Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain Deposited 2016-10-20 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–2163（2163 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1 M Tris-HCl pH 7.5, 10.5 % (w/v) PEG 3350, 0.2 M MgCl
Resolution 3.40 Å R-free 0.296
5M52 Crystal structure of yeast Brr2 full-lenght in complex with Prp8 Jab1 domain Deposited 2016-10-20 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–2163（2163 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291.15 K；0.1 M Tris-HCl pH 7.5, 10.5 % (w/v) PEG 3350, 0.2 M MgCl
Resolution 3.40 Å R-free 0.296
5M5P S. cerevisiae spliceosomal helicase Brr2 (271-end) in complex with the Jab/MPN domain of S. cerevisiae Prp8 Deposited 2016-10-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 271–2163（1893 aa） Fragment：UNP residues 271-2163
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；0.1 M MES pH 6.5 9.2% (w/v) PEG 4000 0.4 M MgCl2
Resolution 4.20 Å R-free 0.335
5M5P S. cerevisiae spliceosomal helicase Brr2 (271-end) in complex with the Jab/MPN domain of S. cerevisiae Prp8 Deposited 2016-10-22 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 271–2163（1893 aa） Fragment：UNP residues 271-2163
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；0.1 M MES pH 6.5 9.2% (w/v) PEG 4000 0.4 M MgCl2
Resolution 4.20 Å R-free 0.335
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain D 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain D 1–2163（2163 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric
Chain B 1–2163（2163 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å

17 other PDB entries and 19 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name BRR2_YEAST
Isoform —
PDB entities 9
Chains and sequence ranges Author chain B； PDBConstruct 1–2163； UniProt 1–2163

Pre-mRNA-splicing factor SNU114

OrganismNot specified

UniProt P36048

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain C； UniProt 1–1008 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain H 1–1008（1008 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric
Chain C 1–1008（1008 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain C 1–1008（1008 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain C 1–1008（1008 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain C 1–1008（1008 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain C 1–1008（1008 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain C 1–1008（1008 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain B 1–1008（1008 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric
Chain C 1–1008（1008 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain C 1–1008（1008 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain C 1–1008（1008 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain C 1–1008（1008 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain C 1–1008（1008 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain C 1–1008（1008 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain C 1–1008（1008 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain B 1–1008（1008 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain C 1–1008（1008 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain C 1–1008（1008 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain C 1–1008（1008 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain C 1–1008（1008 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain C 1–1008（1008 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6TEO Crystal structure of a yeast Snu114-Prp8 complex Deposited 2019-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 72–1008（937 aa）
Not recorded SO4 SULFATE ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；10% (w/v) PEG8000, 200mM Lithium chloride, 50mM magnesium sulfate
Resolution 3.10 Å R-free 0.271
6TEO Crystal structure of a yeast Snu114-Prp8 complex Deposited 2019-11-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 72–1008（937 aa）
Not recorded SO4 SULFATE ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295.15 K；10% (w/v) PEG8000, 200mM Lithium chloride, 50mM magnesium sulfate
Resolution 3.10 Å R-free 0.271
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain C 1–1008（1008 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

23 other PDB entries and 24 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SN114_YEAST
Isoform —
PDB entities 10
Chains and sequence ranges Author chain C； PDBConstruct 1–1008； UniProt 1–1008

Spliceosomal protein DIB1

OrganismNot specified

UniProt Q06819

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain D； UniProt 1–143 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain L 1–143（143 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain D 1–143（143 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAP Body region of the U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Dodecameric
Chain D 1–143（143 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted at 4 deg C for 2 seconds before plunging.
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain E 1–143（143 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain E 1–143（143 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name DIB1_YEAST
Isoform —
PDB entities 11
Chains and sequence ranges Author chain D； PDBConstruct 1–143； UniProt 1–143

66 kDa U4/U6.U5 small nuclear ribonucleoprotein component

OrganismNot specified

UniProt Q12420

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain E； UniProt 1–587 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3PLU Structure of Hub-1 protein in complex with Snu66 peptide (HINDI) Deposited 2010-11-15 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 6–24（19 aa） Fragment：HINDI domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.9；290 K；20% PEG 3350, 0.2M Ammonium Chloride, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 290K
Resolution 1.40 Å R-free 0.228
3PLU Structure of Hub-1 protein in complex with Snu66 peptide (HINDI) Deposited 2010-11-15 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 6–24（19 aa） Fragment：HINDI domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.9；290 K；20% PEG 3350, 0.2M Ammonium Chloride, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 290K
Resolution 1.40 Å R-free 0.228
3PLV Structure of Hub-1 protein in complex with Snu66 peptide (HINDII) Deposited 2010-11-15 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 37–57（21 aa） Fragment：HINDII domain
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.9；290 K；0.2M Ammonium Iodide, 20% PEG 3350, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 290K
Resolution 1.90 Å R-free 0.242
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain O 1–587（587 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain O 1–587（587 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å

4 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SNU66_YEAST
Isoform —
PDB entities 12
Chains and sequence ranges Author chain E； PDBConstruct 1–587； UniProt 1–587

Pre-mRNA-processing factor 31

OrganismNot specified

UniProt P49704

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain F； UniProt 1–494 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain I 1–494（494 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain F 1–494（494 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAP Body region of the U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Dodecameric
Chain F 1–494（494 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted at 4 deg C for 2 seconds before plunging.
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain L 1–494（494 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain L 1–494（494 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PRP31_YEAST
Isoform —
PDB entities 13
Chains and sequence ranges Author chain F； PDBConstruct 1–494； UniProt 1–494

U4/U6 small nuclear ribonucleoprotein PRP3

OrganismNot specified

UniProt Q03338

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain G； UniProt 1–469 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain K 1–469（469 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4YHU Yeast Prp3 C-terminal fragment 296-469 Deposited 2015-02-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 296–469（174 aa） Fragment：C-terminal fragment, UNP residues 296-469
Not recorded YT3 YTTRIUM (III) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M HEPES, pH 7.5, 10 % PEG 8000
Resolution 2.70 Å R-free 0.262
4YHU Yeast Prp3 C-terminal fragment 296-469 Deposited 2015-02-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 296–469（174 aa） Fragment：C-terminal fragment, UNP residues 296-469
Not recorded YT3 YTTRIUM (III) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M HEPES, pH 7.5, 10 % PEG 8000
Resolution 2.70 Å R-free 0.262
4YHU Yeast Prp3 C-terminal fragment 296-469 Deposited 2015-02-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 296–469（174 aa） Fragment：C-terminal fragment, UNP residues 296-469
Not recorded YT3 YTTRIUM (III) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M HEPES, pH 7.5, 10 % PEG 8000
Resolution 2.70 Å R-free 0.262
4YHV Yeast Prp3 C-terminal fragment 325-469 Deposited 2015-02-27 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 325–469（145 aa） Fragment：C-terminal fragment, UNP residues 325-469
Not recorded ACY ACETIC ACID × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M HEPES, pH 7.5, 10 % PEG 8000
Resolution 2.00 Å R-free 0.221
4YHW Yeast Prp3 (296-469) in complex with fragment of U4/U6 di-snRNA Deposited 2015-02-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: trimeric
Chain A 296–469（174 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M sodium fluoride 0.18 M DL-malic acid, pH 6.8 13.2 % PEG 3350
Resolution 3.25 Å R-free 0.299
4YHW Yeast Prp3 (296-469) in complex with fragment of U4/U6 di-snRNA Deposited 2015-02-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Monomer；Protein × 1 PDB declaration: trimeric
Chain B 296–469（174 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；291 K；0.1 M sodium fluoride 0.18 M DL-malic acid, pH 6.8 13.2 % PEG 3350
Resolution 3.25 Å R-free 0.299
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain G 1–469（469 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAP Body region of the U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Dodecameric
Chain G 1–469（469 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted at 4 deg C for 2 seconds before plunging.
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain J 1–469（469 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain J 1–469（469 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å

8 other PDB entries and 11 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PRP3_YEAST
Isoform —
PDB entities 14
Chains and sequence ranges Author chain G； PDBConstruct 1–469； UniProt 1–469

U4/U6 small nuclear ribonucleoprotein PRP4

OrganismNot specified

UniProt P20053

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain H； UniProt 1–465 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain B 1–465（465 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain H 1–465（465 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAP Body region of the U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Dodecameric
Chain H 1–465（465 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted at 4 deg C for 2 seconds before plunging.
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain K 1–465（465 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain K 1–465（465 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PRP4_YEAST
Isoform —
PDB entities 15
Chains and sequence ranges Author chain H； PDBConstruct 1–465； UniProt 1–465

Pre-mRNA-splicing factor 6

OrganismNot specified

UniProt P19735

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain J； UniProt 1–899 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain G 1–899（899 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain J 1–899（899 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAP Body region of the U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Dodecameric
Chain J 1–899（899 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted at 4 deg C for 2 seconds before plunging.
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain N 1–899（899 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain N 1–899（899 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å

5 other PDB entries and 5 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PRP6_YEAST
Isoform —
PDB entities 17
Chains and sequence ranges Author chain J； PDBConstruct 1–899； UniProt 1–899

13 kDa ribonucleoprotein-associated protein

OrganismNot specified

UniProt P39990

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain K； UniProt 1–126 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1ZWZ Structural comparison of Yeast snoRNP and splicesomal protein snu13p with its homologs Deposited 2005-06-06 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–126（126 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å R-free 0.209
1ZWZ Structural comparison of Yeast snoRNP and splicesomal protein snu13p with its homologs Deposited 2005-06-06 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–126（126 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å R-free 0.209
2ALE Crystal structure of yeast RNA splicing factor Snu13p Deposited 2005-08-05 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.2 M Sodium Acetate, 0.1 M Sodium Cacodylate, 30% PEG 8K, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.80 Å R-free 0.248
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain M 1–126（126 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4NUT Crystal structure of the complex between Snu13p and the PEP domain of Rsa1 Deposited 2013-12-04 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–126（126 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 5；293 K；2.3M ammonium sulfate, 70mM sodium citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 1.55 Å R-free 0.235
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain K 1–126（126 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAP Body region of the U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 9 PDB declaration: Dodecameric
Chain K 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted at 4 deg C for 2 seconds before plunging.
Resolution 3.60 Å
5TZS Architecture of the yeast small subunit processome Deposited 2016-11-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Other combination Heteromer；Protein × 52 PDB declaration: 58-meric
Chain e 1–126（126 aa）
Chain f 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE；Incubated 10 seconds on the grid before blotting (blot time 2.0 seconds, blot force 0) and freezing.
Resolution 5.10 Å
5WLC The complete structure of the small subunit processome Deposited 2017-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 67 PDB declaration: 70-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5WYJ Cryo-EM structure of the 90S small subunit pre-ribosome (Dhr1-depleted, Enp1-TAP, state 1) Deposited 2017-01-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 61 PDB declaration: 64-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.70 Å
5WYK Cryo-EM structure of the 90S small subunit pre-ribosome (Mtr4-depleted, Enp1-TAP) Deposited 2017-01-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 58-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE；blot for 2 seconds before plunging
Resolution 4.50 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain M 1–126（126 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain M 1–126（126 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6KE6 3.4 angstrom cryo-EM structure of yeast 90S small subunit preribosome Deposited 2019-07-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 68 PDB declaration: 71-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE；wait 30s and blot 2s before plunging
Resolution 3.40 Å
6LQP Cryo-EM structure of 90S small subunit preribosomes in transition states (State A) Deposited 2020-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 70 PDB declaration: 73-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6LQQ Cryo-EM structure of 90S small subunit preribosomes in transition states (State B) Deposited 2020-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 67 PDB declaration: 70-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
6LQR Cryo-EM structure of 90S small subunit preribosomes in transition states (State C) Deposited 2020-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 65 PDB declaration: 68-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 8.60 Å
6LQS Cryo-EM structure of 90S small subunit preribosomes in transition states (State D) Deposited 2020-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 76 PDB declaration: 79-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6LQT Cryo-EM structure of 90S small subunit preribosomes in transition states (State E) Deposited 2020-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 58-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.90 Å
6LQU Cryo-EM structure of 90S small subunit preribosomes in transition states (State A1) Deposited 2020-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 69 PDB declaration: 72-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6LQV Cryo-EM structure of 90S small subunit preribosomes in transition states (State C1) Deposited 2020-01-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 58-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.80 Å
6ND4 Conformational switches control early maturation of the eukaryotic small ribosomal subunit Deposited 2018-12-13 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 30-meric
Chain e 1–126（126 aa）
Chain f 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.7
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
6ZQA Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state A (Poly-Ala) Deposited 2020-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 57 PDB declaration: 60-meric
Chain CF 1–126（126 aa）
Chain CG 1–126（126 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
6ZQB Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state B2 Deposited 2020-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 68 PDB declaration: 71-meric
Chain CF 1–126（126 aa）
Chain CG 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6ZQC Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Pre-A1 Deposited 2020-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 68 PDB declaration: 71-meric
Chain CF 1–126（126 aa）
Chain CG 1–126（126 aa）
Not recorded ZN ZINC ION × 2 MG MAGNESIUM ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6ZQD Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Post-A1 Deposited 2020-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 63 PDB declaration: 66-meric
Chain CF 1–126（126 aa）
Chain CG 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
6ZQE Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Dis-A (Poly-Ala) Deposited 2020-07-09 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 62-meric
Chain CF 1–126（126 aa）
Chain CG 1–126（126 aa）
Not recorded ZN ZINC ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 7.10 Å
7AJT Cryo-EM structure of the 90S-exosome super-complex (state Pre-A1-exosome) Deposited 2020-09-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 83-meric
Chain CF 1–126（126 aa）
Chain CG 1–126（126 aa）
Not recorded ZN ZINC ION × 3 MG MAGNESIUM ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7AJU Cryo-EM structure of the 90S-exosome super-complex (state Post-A1-exosome) Deposited 2020-09-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 74 PDB declaration: 78-meric
Chain CF 1–126（126 aa）
Chain CG 1–126（126 aa）
Not recorded ZN ZINC ION × 3 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
7D4I Cryo-EM structure of 90S small ribosomal precursors complex with the DEAH-box RNA helicase Dhr1 (State F) Deposited 2020-09-24 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 79 PDB declaration: 82-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
7D5S Cryo-EM structure of 90S preribosome with inactive Utp24 (state A2) Deposited 2020-09-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 58-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.60 Å
7D5T Cryo-EM structure of 90S preribosome with inactive Utp24 (state F1) Deposited 2020-09-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.00 Å
7D63 Cryo-EM structure of 90S preribosome with inactive Utp24 (state C) Deposited 2020-09-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 68 PDB declaration: 71-meric
Chain 3G 1–126（126 aa）
Chain 3H 1–126（126 aa）
Not recorded ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 12.30 Å
9G33 Stalled 90S - Utp23-Krr1-deltaC3 Deposited 2024-07-11 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 62 PDB declaration: 65-meric
Chain CF 1–126（126 aa）
Chain CG 1–126（126 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.05 Å
9N6V SSU processome maturation and disassembly, State A Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 69 PDB declaration: 72-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 25 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.16 Å
9N6W SSU processome maturation and disassembly, State A* Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 69 PDB declaration: 72-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 25 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.05 Å
9N6X SSU processome maturation and disassembly, State B Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 69 PDB declaration: 72-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 34 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.71 Å
9N6Y SSU processome maturation and disassembly, State C Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 70 PDB declaration: 73-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 34 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.65 Å
9N6Z SSU processome maturation and disassembly, State D Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 69 PDB declaration: 72-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.74 Å
9N70 SSU processome maturation and disassembly, State E Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 69 PDB declaration: 72-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.17 Å
9N72 SSU processome maturation and disassembly, State F Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 70 PDB declaration: 73-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.94 Å
9N73 SSU processome maturation and disassembly, State G Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 71 PDB declaration: 74-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.96 Å
9N74 SSU processome maturation and disassembly, State H Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 68 PDB declaration: 71-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 39 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.65 Å
9N75 SSU processome maturation and disassembly, State I Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 65 PDB declaration: 68-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 59 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 3 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.01 Å
9N76 SSU processome maturation and disassembly, State J Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 64 PDB declaration: 67-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 38 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.20 Å
9N77 SSU processome maturation and disassembly, State K Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 61 PDB declaration: 64-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 48 ZN ZINC ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.94 Å
9N78 SSU processome maturation and disassembly, State L Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 59-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 47 ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.17 Å
9N79 SSU processome maturation and disassembly, State M Deposited 2025-02-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 45-meric
Chain SE 1–126（126 aa）
Chain SF 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 47 ZN ZINC ION × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.93 Å

47 other PDB entries and 48 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SNU13_YEAST
Isoform —
PDB entities 18
Chains and sequence ranges Author chain K； PDBConstruct 1–126； UniProt 1–126

23 kDa U4/U6.U5 small nuclear ribonucleoprotein component

OrganismNot specified

UniProt Q12368

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain L； UniProt 1–194 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain W 1–194（194 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SNU23_YEAST
Isoform —
PDB entities 19
Chains and sequence ranges Author chain L； PDBConstruct 1–194； UniProt 1–194

Pre-mRNA-splicing factor 38

OrganismNot specified

UniProt Q00723

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain M； UniProt 1–242 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain 0 1–242（242 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PRP38_YEAST
Isoform —
PDB entities 20
Chains and sequence ranges Author chain M； PDBConstruct 1–242； UniProt 1–242

Pre-mRNA-splicing factor SPP381

OrganismNot specified

UniProt P38282

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain N； UniProt 1–291 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain 9 1–291（291 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å

1 other PDB entries and 1 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SP381_YEAST
Isoform —
PDB entities 21
Chains and sequence ranges Author chain N； PDBConstruct 1–291； UniProt 1–291

U2 snRNP component HSH155

OrganismNot specified

UniProt P49955

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain O； UniProt 1–971 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain G 1–971（971 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain Q 1–971（971 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain 1 1–971（971 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain 1 1–971（971 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain O 1–971（971 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain O 1–971（971 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain O 1–971（971 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SF3B1_YEAST
Isoform —
PDB entities 22
Chains and sequence ranges Author chain O； PDBConstruct 1–971； UniProt 1–971

Pre-mRNA-splicing factor RSE1

OrganismNot specified

UniProt Q04693

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain P； UniProt 1–1361 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain F 1–1361（1361 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain X 1–1361（1361 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain 3 1–1361（1361 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain 3 1–1361（1361 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain P 1–1361（1361 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain P 1–1361（1361 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain P 1–1361（1361 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSE1_YEAST
Isoform —
PDB entities 23
Chains and sequence ranges Author chain P； PDBConstruct 1–1361； UniProt 1–1361

Cold sensitive U2 snRNA suppressor 1

OrganismNot specified

UniProt Q02554

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain Q； UniProt 1–435 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain H 1–436（436 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5LSB Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-08-24 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 290–368（79 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Equal volumes of 10-15 mg/ml protein solution were mixed with an equal volume of reservoir solution containing 2.45-2.65 M NaCl in 0.1M sodium acetate pH 4.8-4.9
Resolution 2.70 Å R-free 0.233
5LSB Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-08-24 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 290–368（79 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Equal volumes of 10-15 mg/ml protein solution were mixed with an equal volume of reservoir solution containing 2.45-2.65 M NaCl in 0.1M sodium acetate pH 4.8-4.9
Resolution 2.70 Å R-free 0.233
5LSB Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-08-24 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 290–368（79 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Equal volumes of 10-15 mg/ml protein solution were mixed with an equal volume of reservoir solution containing 2.45-2.65 M NaCl in 0.1M sodium acetate pH 4.8-4.9
Resolution 2.70 Å R-free 0.233
5LSL Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 290–368（79 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；18% PEG4000, 0.1M Tris HCl pH 8.5, 45 mM LiSO4
Resolution 1.65 Å R-free 0.217
5LSL Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 290–368（79 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；18% PEG4000, 0.1M Tris HCl pH 8.5, 45 mM LiSO4
Resolution 1.65 Å R-free 0.217
5LSL Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 290–368（79 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；18% PEG4000, 0.1M Tris HCl pH 8.5, 45 mM LiSO4
Resolution 1.65 Å R-free 0.217
5LSL Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain H 290–368（79 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；18% PEG4000, 0.1M Tris HCl pH 8.5, 45 mM LiSO4
Resolution 1.65 Å R-free 0.217
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain 2 1–436（436 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain 2 1–436（436 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain Q 1–435（435 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain Q 1–436（436 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Q 1–436（436 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å

8 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CUS1_YEAST
Isoform —
PDB entities 24
Chains and sequence ranges Author chain Q； PDBConstruct 1–435； UniProt 1–435

Protein HSH49

OrganismNot specified

UniProt Q99181

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain R； UniProt 1–213 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain e 1–213（213 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5LSB Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-08-24 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 2–213（212 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Equal volumes of 10-15 mg/ml protein solution were mixed with an equal volume of reservoir solution containing 2.45-2.65 M NaCl in 0.1M sodium acetate pH 4.8-4.9
Resolution 2.70 Å R-free 0.233
5LSB Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-08-24 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 2–213（212 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Equal volumes of 10-15 mg/ml protein solution were mixed with an equal volume of reservoir solution containing 2.45-2.65 M NaCl in 0.1M sodium acetate pH 4.8-4.9
Resolution 2.70 Å R-free 0.233
5LSB Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-08-24 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain F 2–213（212 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Equal volumes of 10-15 mg/ml protein solution were mixed with an equal volume of reservoir solution containing 2.45-2.65 M NaCl in 0.1M sodium acetate pH 4.8-4.9
Resolution 2.70 Å R-free 0.233
5LSL Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 2–100（99 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；18% PEG4000, 0.1M Tris HCl pH 8.5, 45 mM LiSO4
Resolution 1.65 Å R-free 0.217
5LSL Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 2–100（99 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；18% PEG4000, 0.1M Tris HCl pH 8.5, 45 mM LiSO4
Resolution 1.65 Å R-free 0.217
5LSL Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 2–100（99 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；18% PEG4000, 0.1M Tris HCl pH 8.5, 45 mM LiSO4
Resolution 1.65 Å R-free 0.217
5LSL Crystal structure of yeast Hsh49p in complex with Cus1p binding domain. Deposited 2016-09-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain D 2–100（99 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；18% PEG4000, 0.1M Tris HCl pH 8.5, 45 mM LiSO4
Resolution 1.65 Å R-free 0.217
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain 4 1–213（213 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain 4 1–213（213 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain R 1–213（213 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain R 1–213（213 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain R 1–213（213 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å

8 other PDB entries and 13 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name HSH49_YEAST
Isoform —
PDB entities 25
Chains and sequence ranges Author chain R； PDBConstruct 1–213； UniProt 1–213

Pre-mRNA-splicing factor RDS3

OrganismNot specified

UniProt Q06835

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain S； UniProt 1–107 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2K0A 1H, 15N and 13C chemical shift assignments for Rds3 protein Deposited 2008-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–107（106 aa）
Not recorded ZN ZINC ION × 3 SOLUTION NMR
NMR measurement conditions pH 7；300 K；Ionic strength (raw mmCIF value) 200；Pressure ambient
NMR sample composition 350 uM [U-98% 15N] Rds3p, 20 mM [U-99% 2H] Tris buffer, 200 mM sodium chloride, 1 mM [U-99% 2H] DTT, 93% H2O/7% D2O | 93% H2O/7% D2O
NMR sample composition 700 uM [U-98% 13C; U-98% 15N] Rds3p, 20 mM [U-99% 2H] Tris buffer, 200 mM sodium chloride, 1 mM [U-99% 2H] DTT, 93% H2O/7% D2O | 93% H2O/7% D2O
Resolution not provided
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain J 1–107（107 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain Y 1–107（107 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain 5 1–107（107 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain 5 1–107（107 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain S 1–107（107 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain S 1–107（107 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain S 1–107（107 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RDS3_YEAST
Isoform —
PDB entities 26
Chains and sequence ranges Author chain S； PDBConstruct 1–107； UniProt 1–107

Pre-mRNA-splicing factor PRP9

OrganismNot specified

UniProt P19736

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain T； UniProt 1–530 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4DGW Crystal Structure of the SF3a splicing factor complex of U2 snRNP Deposited 2012-01-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain A 1–389（389 aa） Fragment：N-terminal domain, UNP residues 1-389
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；290 K；8-12% Ethanol, 100mM MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution 3.11 Å R-free 0.278
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain u 1–530（530 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain u 1–530（530 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain T 1–530（530 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain T 1–530（530 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain T 1–530（530 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å

6 other PDB entries and 6 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PRP9_YEAST
Isoform —
PDB entities 27
Chains and sequence ranges Author chain T； PDBConstruct 1–530； UniProt 1–530

Pre-mRNA-splicing factor PRP11

OrganismNot specified

UniProt Q07350

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain U； UniProt 1–266 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4DGW Crystal Structure of the SF3a splicing factor complex of U2 snRNP Deposited 2012-01-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain C 50–266（217 aa） Fragment：C-terminal domain, UNP residues 50-266
Mutation：L153M Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；290 K；8-12% Ethanol, 100mM MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution 3.11 Å R-free 0.278
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain I 1–266（266 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain v 1–266（266 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain v 1–266（266 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain U 1–114（114 aa）
Chain U 137–266（130 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain U 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain U 1–266（266 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PRP11_YEAST
Isoform —
PDB entities 28
Chains and sequence ranges Author chain U； PDBConstruct 1–266； UniProt 1–266

Pre-mRNA-splicing factor PRP21

OrganismNot specified

UniProt P32524

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain V； UniProt 1–280 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4DGW Crystal Structure of the SF3a splicing factor complex of U2 snRNP Deposited 2012-01-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric
Chain B 87–237（151 aa） Fragment：Middle domain, UNP residues 87-237
Non-standard monomer：Yes (specific site not provided by mmCIF) ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6；290 K；8-12% Ethanol, 100mM MES, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 290K
Resolution 3.11 Å R-free 0.278
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain w 1–280（280 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain w 1–280（280 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain V 1–280（280 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
7DCO Cryo-EM structure of the activated spliceosome (Bact complex) at an atomic resolution of 2.5 angstrom Deposited 2020-10-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric
Chain w 1–280（280 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 CA CALCIUM ION × 1 ZN ZINC ION × 15 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain V 1–280（280 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain V 1–280（280 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å

7 other PDB entries and 7 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name PRP21_YEAST
Isoform —
PDB entities 29
Chains and sequence ranges Author chain V； PDBConstruct 1–280； UniProt 1–280

;U2 small nuclear ribonucleoprotein A' ;

OrganismNot specified

UniProt Q08963

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain W； UniProt 1–238 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain b 1–238（238 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain W 1–238（238 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain W 1–238（238 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain W 1–238（238 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain Y 1–238（238 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain o 1–238（238 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain o 1–238（238 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain o 1–238（238 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain o 1–238（238 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain s 1–238（238 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain W 1–238（238 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain W 1–238（238 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain b 1–238（238 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain b 1–238（238 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain b 1–238（238 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain b 1–238（238 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain W 1–238（238 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain W 1–238（238 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain W 1–238（238 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

19 other PDB entries and 19 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RU2A_YEAST
Isoform —
PDB entities 30
Chains and sequence ranges Author chain W； PDBConstruct 1–238； UniProt 1–238

;U2 small nuclear ribonucleoprotein B'' ;

OrganismNot specified

UniProt P40567

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain Y； UniProt 1–111 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain a 1–111（111 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain Y 1–111（111 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain Y 1–111（111 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain Y 1–111（111 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain X 1–111（111 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain p 1–111（111 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain p 1–111（111 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain p 1–111（111 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain p 1–111（111 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain r 1–111（111 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain Y 1–111（111 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain Y 1–111（111 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain a 1–111（111 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain a 1–111（111 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain a 1–111（111 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain a 1–111（111 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain Y 1–111（111 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Y 1–111（111 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain Y 1–111（111 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

19 other PDB entries and 19 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name MSL1_YEAST
Isoform —
PDB entities 32
Chains and sequence ranges Author chain Y； PDBConstruct 1–111； UniProt 1–111

RDS3 complex subunit 10

OrganismNot specified

UniProt P0C074

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain Z； UniProt 1–85 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain K 1–85（85 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain Z 1–85（85 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain 6 1–85（85 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain 6 1–85（85 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain Z 1–85（85 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
7DCO Cryo-EM structure of the activated spliceosome (Bact complex) at an atomic resolution of 2.5 angstrom Deposited 2020-10-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric
Chain 6 1–84（84 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 CA CALCIUM ION × 1 ZN ZINC ION × 15 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain Z 1–84（84 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain Z 1–85（85 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å

8 other PDB entries and 8 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name YSF3_YEAST
Isoform —
PDB entities 33
Chains and sequence ranges Author chain Z； PDBConstruct 1–85； UniProt 1–85

U6 snRNA-associated Sm-like protein LSm2

OrganismNot specified

UniProt P38203

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain a； UniProt 1–95 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain c 1–95（95 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4C8Q Crystal structure of the yeast Lsm1-7-Pat1 complex Deposited 2013-10-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 2–95（94 aa） Fragment：RESIDUES 2-95
Not recorded CO COBALT (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM HEPES PH 7.0, 20% MPD, 10 MM HEXAMINE COBALT(III) CHLORIDE
Resolution 3.70 Å R-free 0.295
4C92 Crystal structure of the yeast Lsm1-7 complex Deposited 2013-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 2–95（94 aa） Fragment：RESIDUES 2-95
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM MES PH 6.0, 40% MPD
Resolution 2.30 Å R-free 0.259
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–95（95 aa）
Mutation：C45S Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain I 1–95（95 aa）
Mutation：C45S Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–95（95 aa）
Mutation：C45S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain I 1–95（95 aa）
Mutation：C45S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain B 1–95（95 aa）
Mutation：C45S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain I 1–95（95 aa）
Mutation：C45S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain I 1–95（95 aa）
Mutation：C45S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain B 1–95（95 aa）
Mutation：C45S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein–RNA Heteromer；Protein × 14 PDB declaration: hexadecameric
Chain B 1–95（95 aa）
Chain I 1–95（95 aa）
Mutation：C45S Mutation：C45S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain B 1–95（95 aa）
Mutation：C45S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain I 1–95（95 aa）
Mutation：C45S No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
4N0A Crystal structure of Lsm2-3-Pat1C complex from Saccharomyces cerevisiae Deposited 2013-10-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 14 PDB declaration: tetradecameric
Chain C 1–95（95 aa）
Chain D 1–95（95 aa）
Chain G 1–95（95 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；288 K；0.1M Tris, 12% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K
Resolution 3.15 Å R-free 0.300
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain 2 1–95（95 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5VSU Structure of yeast U6 snRNP with 2'-phosphate terminated U6 RNA Deposited 2017-05-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain B 1–95（95 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；289 K；0.2 M NH4F 0.1 M HEPES pH 7.4 0.01 M MgCl2 18 % PEG 3,350
Resolution 3.10 Å R-free 0.298
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain q 1–95（95 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain q 1–95（95 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6ASO Structure of yeast U6 snRNP with 3'-phosphate terminated U6 RNA Deposited 2017-08-25 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain B 1–95（95 aa）
Not recorded K POTASSIUM ION × 2 MN MANGANESE (II) ION × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；280 mM sodium potassium tartrate 22.5 % glycerol 17.5 % PEG 3,350 1 mM manganese chloride
Resolution 2.71 Å R-free 0.247

14 other PDB entries and 20 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LSM2_YEAST
Isoform —
PDB entities 34
Chains and sequence ranges Author chain a； PDBConstruct 1–95； UniProt 1–95

Small nuclear ribonucleoprotein-associated protein B

OrganismNot specified

UniProt P40018

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain b； UniProt 1–196 Chain k； UniProt 1–196 Chain s； UniProt 1–196 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain O 1–196（196 aa）
Chain S 1–196（196 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric
Chain b 1–196（196 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain b 1–196（196 aa）
Chain k 1–196（196 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric
Chain k 1–196（196 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain k 1–196（196 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain k 1–196（196 aa）
Chain s 1–196（196 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain b 1–196（196 aa）
Chain k 1–196（196 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain b 1–196（196 aa）
Chain k 1–196（196 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain b 1–196（196 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric
Chain b 1–196（196 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain b 1–196（196 aa）
Chain k 1–196（196 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain F 1–196（196 aa）
Chain k 1–196（196 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain a 1–196（196 aa）
Chain h 1–196（196 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain a 1–196（196 aa）
Chain h 1–196（196 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain P 1–196（196 aa）
Chain a 1–196（196 aa）
Chain h 1–196（196 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWN Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region) Deposited 2018-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: eicosameric
Chain a 1–196（196 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain P 1–196（196 aa）
Chain a 1–196（196 aa）
Chain h 1–196（196 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain f 1–196（196 aa）
Chain k 1–196（196 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain b 1–196（196 aa）
Chain k 1–196（196 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain b 1–196（196 aa）
Chain s 1–196（196 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain k 1–196（196 aa）
Chain s 1–196（196 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain k 1–196（196 aa）
Chain s 1–196（196 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain k 1–196（196 aa）
Chain s 1–196（196 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain k 1–196（196 aa）
Chain s 1–196（196 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6N7P S. cerevisiae spliceosomal E complex (UBC4) Deposited 2018-11-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain K 1–196（196 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6N7R Saccharomyces cerevisiae spliceosomal E complex (ACT1) Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain K 1–196（196 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6N7X S. cerevisiae U1 snRNP Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric
Chain K 1–196（196 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric
Chain b 1–196（196 aa）
Chain k 1–196（196 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain s 1–196（196 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQC The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain b 1–196（196 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain b 1–196（196 aa）
Chain s 1–196（196 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
8W2O Yeast U1 snRNP with humanized U1C Zinc-Finger domain Deposited 2024-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 18-meric
Chain K 1–196（196 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；20 mM Hepes, pH7.9, 120 mM KCl, 2 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；The grids were obtained with the chamber at 100% humidity, 2.5 s blotting time, -6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific)
Resolution 3.49 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain b 1–196（196 aa）
Chain k 1–196（196 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

33 other PDB entries and 33 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RSMB_YEAST
Isoform —
PDB entities 35
Chains and sequence ranges Author chain b； PDBConstruct 1–196； UniProt 1–196 Author chain k； PDBConstruct 1–196； UniProt 1–196 Author chain s； PDBConstruct 1–196； UniProt 1–196

Small nuclear ribonucleoprotein Sm D3

OrganismNot specified

UniProt P43321

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain d； UniProt 1–101 Chain n； UniProt 1–101 Chain v； UniProt 1–101 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain J 1–101（101 aa）
Chain R 1–101（101 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric
Chain d 1–101（101 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain d 1–101（101 aa）
Chain n 1–101（101 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric
Chain n 1–101（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain l 1–101（101 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain l 1–101（101 aa）
Chain y 1–101（101 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain d 1–101（101 aa）
Chain n 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain d 1–101（101 aa）
Chain n 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain d 1–101（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric
Chain d 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain d 1–101（101 aa）
Chain n 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain U 1–101（101 aa）
Chain l 1–101（101 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain e 1–101（101 aa）
Chain l 1–101（101 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain e 1–101（101 aa）
Chain l 1–101（101 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain S 1–101（101 aa）
Chain d 1–101（101 aa）
Chain l 1–101（101 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWN Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region) Deposited 2018-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: eicosameric
Chain d 1–101（101 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain S 1–101（101 aa）
Chain d 1–101（101 aa）
Chain l 1–101（101 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain g 1–101（101 aa）
Chain o 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain d 1–101（101 aa）
Chain n 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain d 1–101（101 aa）
Chain v 1–101（101 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain l 1–101（101 aa）
Chain y 1–101（101 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain l 1–101（101 aa）
Chain y 1–101（101 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain l 1–101（101 aa）
Chain y 1–101（101 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain l 1–101（101 aa）
Chain y 1–101（101 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6N7P S. cerevisiae spliceosomal E complex (UBC4) Deposited 2018-11-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain N 1–101（101 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6N7R Saccharomyces cerevisiae spliceosomal E complex (ACT1) Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain N 1–101（101 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6N7X S. cerevisiae U1 snRNP Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric
Chain N 1–101（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric
Chain d 1–101（101 aa）
Chain n 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain v 1–101（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQC The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain d 1–101（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain d 1–101（101 aa）
Chain v 1–101（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
8W2O Yeast U1 snRNP with humanized U1C Zinc-Finger domain Deposited 2024-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 18-meric
Chain N 1–101（101 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；20 mM Hepes, pH7.9, 120 mM KCl, 2 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；The grids were obtained with the chamber at 100% humidity, 2.5 s blotting time, -6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific)
Resolution 3.49 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain d 1–101（101 aa）
Chain n 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

33 other PDB entries and 33 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SMD3_YEAST
Isoform —
PDB entities 36
Chains and sequence ranges Author chain d； PDBConstruct 1–101； UniProt 1–101 Author chain n； PDBConstruct 1–101； UniProt 1–101 Author chain v； PDBConstruct 1–101； UniProt 1–101

Small nuclear ribonucleoprotein E

OrganismNot specified

UniProt Q12330

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain e； UniProt 1–94 Chain p； UniProt 1–94 Chain w； UniProt 1–94 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain V 1–94（94 aa）
Chain Y 1–94（94 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric
Chain e 1–94（94 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain e 1–94（94 aa）
Chain p 1–94（94 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric
Chain p 1–94（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain i 1–94（94 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain i 1–94（94 aa）
Chain u 1–94（94 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain e 1–94（94 aa）
Chain p 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain e 1–94（94 aa）
Chain p 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain e 1–94（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric
Chain e 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain e 1–94（94 aa）
Chain p 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain G 1–94（94 aa）
Chain i 1–94（94 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain b 1–94（94 aa）
Chain i 1–94（94 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain b 1–94（94 aa）
Chain i 1–94（94 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain T 1–94（94 aa）
Chain e 1–94（94 aa）
Chain i 1–94（94 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWN Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region) Deposited 2018-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: eicosameric
Chain e 1–94（94 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain T 1–94（94 aa）
Chain e 1–94（94 aa）
Chain i 1–94（94 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain c 1–94（94 aa）
Chain l 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain e 1–94（94 aa）
Chain p 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain e 1–94（94 aa）
Chain w 1–94（94 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain i 1–94（94 aa）
Chain u 1–94（94 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain i 1–94（94 aa）
Chain u 1–94（94 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain i 1–94（94 aa）
Chain u 1–94（94 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain i 1–94（94 aa）
Chain u 1–94（94 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6N7P S. cerevisiae spliceosomal E complex (UBC4) Deposited 2018-11-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain O 1–94（94 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6N7R Saccharomyces cerevisiae spliceosomal E complex (ACT1) Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain O 1–94（94 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6N7X S. cerevisiae U1 snRNP Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric
Chain O 1–94（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric
Chain e 1–94（94 aa）
Chain p 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain w 1–93（93 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQC The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain e 1–94（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain e 1–94（94 aa）
Chain w 1–94（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
8W2O Yeast U1 snRNP with humanized U1C Zinc-Finger domain Deposited 2024-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 18-meric
Chain O 1–94（94 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；20 mM Hepes, pH7.9, 120 mM KCl, 2 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；The grids were obtained with the chamber at 100% humidity, 2.5 s blotting time, -6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific)
Resolution 3.49 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain e 1–94（94 aa）
Chain p 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

33 other PDB entries and 33 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RUXE_YEAST
Isoform —
PDB entities 37
Chains and sequence ranges Author chain e； PDBConstruct 1–94； UniProt 1–94 Author chain p； PDBConstruct 1–94； UniProt 1–94 Author chain w； PDBConstruct 1–94； UniProt 1–94

Small nuclear ribonucleoprotein F

OrganismNot specified

UniProt P54999

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain f； UniProt 1–86 Chain q； UniProt 1–86 Chain x； UniProt 1–86 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1N9R Crystal structure of a heptameric ring complex of yeast SmF in spacegroup P4122 Deposited 2002-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric
Chain A 1–86（86 aa）
Chain B 1–86（86 aa）
Chain C 1–86（86 aa）
Chain D 1–86（86 aa）
Chain E 1–86（86 aa）
Chain F 1–86（86 aa）
Chain G 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 2.80 Å R-free 0.268
1N9R Crystal structure of a heptameric ring complex of yeast SmF in spacegroup P4122 Deposited 2002-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric
Chain A 1–86（86 aa）
Chain B 1–86（86 aa）
Chain C 1–86（86 aa）
Chain D 1–86（86 aa）
Chain E 1–86（86 aa）
Chain F 1–86（86 aa）
Chain G 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 2.80 Å R-free 0.268
1N9S Crystal structure of yeast SmF in spacegroup P43212 Deposited 2002-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric
Chain A 1–86（86 aa）
Chain B 1–86（86 aa）
Chain C 1–86（86 aa）
Chain D 1–86（86 aa）
Chain E 1–86（86 aa）
Chain F 1–86（86 aa）
Chain G 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；Tris, PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 3.50 Å R-free 0.297
1N9S Crystal structure of yeast SmF in spacegroup P43212 Deposited 2002-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 7 PDB declaration: heptameric
Chain H 1–86（86 aa）
Chain I 1–86（86 aa）
Chain J 1–86（86 aa）
Chain K 1–86（86 aa）
Chain L 1–86（86 aa）
Chain M 1–86（86 aa）
Chain N 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；Tris, PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 3.50 Å R-free 0.297
1N9S Crystal structure of yeast SmF in spacegroup P43212 Deposited 2002-11-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 14 PDB declaration: tetradecameric
Chain A 1–86（86 aa）
Chain B 1–86（86 aa）
Chain C 1–86（86 aa）
Chain D 1–86（86 aa）
Chain E 1–86（86 aa）
Chain F 1–86（86 aa）
Chain G 1–86（86 aa）
Chain H 1–86（86 aa）
Chain I 1–86（86 aa）
Chain J 1–86（86 aa）
Chain K 1–86（86 aa）
Chain L 1–86（86 aa）
Chain M 1–86（86 aa）
Chain N 1–86（86 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8.5；289 K；Tris, PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Resolution 3.50 Å R-free 0.297
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain W 1–86（86 aa）
Chain Z 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric
Chain f 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric
Chain q 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain h 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain f 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric
Chain f 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain H 1–86（86 aa）
Chain h 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain c 1–86（86 aa）
Chain j 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain c 1–86（86 aa）
Chain j 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain U 1–86（86 aa）
Chain f 1–86（86 aa）
Chain j 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWN Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region) Deposited 2018-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: eicosameric
Chain f 1–86（86 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain U 1–86（86 aa）
Chain f 1–86（86 aa）
Chain j 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain b 1–86（86 aa）
Chain m 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain f 1–86（86 aa）
Chain x 1–86（86 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain h 1–86（86 aa）
Chain w 1–86（86 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6N7P S. cerevisiae spliceosomal E complex (UBC4) Deposited 2018-11-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain P 1–86（86 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6N7R Saccharomyces cerevisiae spliceosomal E complex (ACT1) Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain P 1–86（86 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6N7X S. cerevisiae U1 snRNP Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric
Chain P 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain x 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQC The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain f 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain f 1–86（86 aa）
Chain x 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
8W2O Yeast U1 snRNP with humanized U1C Zinc-Finger domain Deposited 2024-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 18-meric
Chain P 1–86（86 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；20 mM Hepes, pH7.9, 120 mM KCl, 2 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；The grids were obtained with the chamber at 100% humidity, 2.5 s blotting time, -6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific)
Resolution 3.49 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain f 1–86（86 aa）
Chain q 1–86（86 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

35 other PDB entries and 38 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RUXF_YEAST
Isoform —
PDB entities 38
Chains and sequence ranges Author chain f； PDBConstruct 1–86； UniProt 1–86 Author chain q； PDBConstruct 1–86； UniProt 1–86 Author chain x； PDBConstruct 1–86； UniProt 1–86

Small nuclear ribonucleoprotein G

OrganismNot specified

UniProt P40204

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain g； UniProt 1–77 Chain r； UniProt 1–77 Chain y； UniProt 1–77 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain X 1–77（77 aa）
Chain a 1–77（77 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric
Chain g 1–77（77 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain g 1–77（77 aa）
Chain r 1–77（77 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric
Chain r 1–77（77 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain j 1–77（77 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain j 1–77（77 aa）
Chain x 1–77（77 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain g 1–77（77 aa）
Chain r 1–77（77 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain g 1–77（77 aa）
Chain r 1–77（77 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain g 1–77（77 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric
Chain g 1–77（77 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain g 1–77（77 aa）
Chain r 1–77（77 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain K 1–77（77 aa）
Chain j 1–77（77 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain d 1–77（77 aa）
Chain k 1–77（77 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain d 1–77（77 aa）
Chain k 1–77（77 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain V 1–77（77 aa）
Chain g 1–77（77 aa）
Chain k 1–77（77 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWN Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region) Deposited 2018-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: eicosameric
Chain g 1–77（77 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain V 1–77（77 aa）
Chain g 1–77（77 aa）
Chain k 1–77（77 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain d 1–77（77 aa）
Chain n 1–77（77 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain g 1–77（77 aa）
Chain r 1–77（77 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain g 1–77（77 aa）
Chain y 1–77（77 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain j 1–77（77 aa）
Chain x 1–77（77 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain j 1–77（77 aa）
Chain x 1–77（77 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain j 1–77（77 aa）
Chain x 1–77（77 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain j 1–77（77 aa）
Chain x 1–77（77 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6N7P S. cerevisiae spliceosomal E complex (UBC4) Deposited 2018-11-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain Q 1–77（77 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6N7R Saccharomyces cerevisiae spliceosomal E complex (ACT1) Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain Q 1–77（77 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6N7X S. cerevisiae U1 snRNP Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric
Chain Q 1–77（77 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric
Chain g 1–77（77 aa）
Chain r 1–77（77 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7DCO Cryo-EM structure of the activated spliceosome (Bact complex) at an atomic resolution of 2.5 angstrom Deposited 2020-10-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 56-meric
Chain g 1–77（77 aa）
Chain k 1–77（77 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 CA CALCIUM ION × 1 ZN ZINC ION × 15 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.50 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain y 1–77（77 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQC The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain g 1–77（77 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain g 1–77（77 aa）
Chain y 1–77（77 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
8W2O Yeast U1 snRNP with humanized U1C Zinc-Finger domain Deposited 2024-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 18-meric
Chain Q 1–77（77 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；20 mM Hepes, pH7.9, 120 mM KCl, 2 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；The grids were obtained with the chamber at 100% humidity, 2.5 s blotting time, -6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific)
Resolution 3.49 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain g 1–77（77 aa）
Chain r 1–77（77 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

34 other PDB entries and 34 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RUXG_YEAST
Isoform —
PDB entities 39
Chains and sequence ranges Author chain g； PDBConstruct 1–77； UniProt 1–77 Author chain r； PDBConstruct 1–77； UniProt 1–77 Author chain y； PDBConstruct 1–77； UniProt 1–77

Small nuclear ribonucleoprotein Sm D1

OrganismNot specified

UniProt Q02260

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain h； UniProt 1–146 Chain l； UniProt 1–146 Chain t； UniProt 1–146 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain P 1–146（146 aa）
Chain T 1–146（146 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric
Chain h 1–146（146 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain h 1–146（146 aa）
Chain l 1–146（146 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric
Chain l 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain m 1–146（146 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain m 1–146（146 aa）
Chain z 1–146（146 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain h 1–146（146 aa）
Chain l 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain h 1–146（146 aa）
Chain l 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain h 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric
Chain h 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain h 1–146（146 aa）
Chain l 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain V 1–146（146 aa）
Chain m 1–146（146 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain f 1–146（146 aa）
Chain m 1–146（146 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain f 1–146（146 aa）
Chain m 1–146（146 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain Q 1–146（146 aa）
Chain b 1–146（146 aa）
Chain m 1–146（146 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWN Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region) Deposited 2018-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: eicosameric
Chain b 1–146（146 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain Q 1–146（146 aa）
Chain b 1–146（146 aa）
Chain m 1–146（146 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain h 1–146（146 aa）
Chain p 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain h 1–146（146 aa）
Chain l 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain h 1–146（146 aa）
Chain t 1–146（146 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain m 1–146（146 aa）
Chain z 1–146（146 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain m 1–146（146 aa）
Chain z 1–146（146 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain m 1–146（146 aa）
Chain z 1–146（146 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain m 1–146（146 aa）
Chain z 1–146（146 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6N7P S. cerevisiae spliceosomal E complex (UBC4) Deposited 2018-11-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain L 1–146（146 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6N7R Saccharomyces cerevisiae spliceosomal E complex (ACT1) Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain L 1–146（146 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6N7X S. cerevisiae U1 snRNP Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric
Chain L 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric
Chain h 1–146（146 aa）
Chain l 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain t 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQC The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain h 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain h 1–146（146 aa）
Chain t 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
8W2O Yeast U1 snRNP with humanized U1C Zinc-Finger domain Deposited 2024-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 18-meric
Chain L 1–146（146 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；20 mM Hepes, pH7.9, 120 mM KCl, 2 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；The grids were obtained with the chamber at 100% humidity, 2.5 s blotting time, -6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific)
Resolution 3.49 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain h 1–146（146 aa）
Chain l 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

33 other PDB entries and 33 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SMD1_YEAST
Isoform —
PDB entities 40
Chains and sequence ranges Author chain h； PDBConstruct 1–146； UniProt 1–146 Author chain l； PDBConstruct 1–146； UniProt 1–146 Author chain t； PDBConstruct 1–146； UniProt 1–146

Small nuclear ribonucleoprotein Sm D2

OrganismNot specified

UniProt Q06217

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain i； UniProt 1–110 Chain m； UniProt 1–110 Chain u； UniProt 1–110 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain Q 1–110（110 aa）
Chain U 1–110（110 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5GAM Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: Dodecameric
Chain j 1–110（110 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain j 1–110（110 aa）
Chain m 1–110（110 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5GAO Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 10 PDB declaration: 11-meric
Chain m 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 4.20 Å
5GM6 Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution Deposited 2016-07-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain g 15–108（94 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 5 ZN ZINC ION × 13 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5GMK Cryo-EM structure of the Catalytic Step I spliceosome (C complex) at 3.4 angstrom resolution Deposited 2016-07-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 45-meric
Chain e 1–110（110 aa）
Chain g 1–110（110 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；The CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5LJ3 Structure of the core of the yeast spliceosome immediately after branching Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 33 PDB declaration: 38-meric
Chain j 1–110（110 aa）
Chain m 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 3.80 Å
5LJ5 Overall structure of the yeast spliceosome immediately after branching. Deposited 2016-07-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 45-meric
Chain j 1–110（110 aa）
Chain m 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 2 ZN ZINC ION × 7 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.8
cryo-EM vitrification conditions Cryogen ETHANE；3 microlitres sample were applied to the grid, left for 30 seconds and then blotted for 2.5-3.0 seconds before plunging.
Resolution 10.00 Å
5LQW yeast activated spliceosome Deposited 2016-08-17 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 27 PDB declaration: 31-meric
Chain j 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.3
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.80 Å
5MPS Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-18 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 25 PDB declaration: 30-meric
Chain j 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 3.85 Å
5MQ0 Structure of a spliceosome remodeled for exon ligation Deposited 2016-12-19 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 40 PDB declaration: 46-meric
Chain j 1–110（110 aa）
Chain m 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 3 K POTASSIUM ION × 2 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；NP-40 is also called IGEPAL CA-630
cryo-EM vitrification conditions Cryogen ETHANE；3.5 microlitres sample were applied to the grid, left for 25 seconds and then blotted for 3.0-3.5 seconds before plunging.
Resolution 4.17 Å
5WSG Cryo-EM structure of the Catalytic Step II spliceosome (C* complex) at 4.0 angstrom resolution Deposited 2016-12-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 45-meric
Chain W 1–110（110 aa）
Chain g 1–110（110 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8；10mM Tris-HCl, pH 8.0, 75mM NaCl, 1mM Mg(OAc)2, 1mM imidazole, 0.01% NP40, 1mM TCEP, 0.5mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.00 Å
5Y88 Cryo-EM structure of the intron-lariat spliceosome ready for disassembly from S.cerevisiae at 3.5 angstrom Deposited 2017-08-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 44-meric
Chain g 1–110（110 aa）
Chain n 1–110（110 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 8
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.46 Å
5YLZ Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom Deposited 2017-10-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 39 PDB declaration: 43-meric
Chain g 1–110（110 aa）
Chain n 1–110（110 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain R 1–110（110 aa）
Chain c 1–110（110 aa）
Chain n 1–110（110 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWN Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.3 angstrom (Part II: U1 snRNP region) Deposited 2018-05-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 18 PDB declaration: eicosameric
Chain c 1–110（110 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain R 1–110（110 aa）
Chain c 1–110（110 aa）
Chain n 1–110（110 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6BK8 S. cerevisiae spliceosomal post-catalytic P complex Deposited 2017-11-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 46-meric
Chain a 1–110（110 aa）
Chain q 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 5 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.30 Å
6EXN Post-catalytic P complex spliceosome with 3' splice site docked Deposited 2017-11-08 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 41 PDB declaration: 40-meric
Chain j 1–110（110 aa）
Chain m 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 1 IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；3 uL sample was applied to the grid, left for 30s, then blotted for 3s and immediately plunged into liquid ethane.
Resolution 3.70 Å
6G90 Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2) Deposited 2018-04-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 35 PDB declaration: 38-meric
Chain i 1–110（110 aa）
Chain u 1–110（110 aa）
Not recorded ZN ZINC ION × 9 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2-3.5 s and vitrified by plunging into liquid ethane with a FEI Vitrobot Mark III operated at 4 degree Celsius and 100% humidity.
Resolution 4.00 Å
6J6G Cryo-EM structure of the yeast B*-a2 complex at an average resolution of 3.2 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain e 1–110（110 aa）
Chain g 1–110（110 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6J6H Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain e 1–110（110 aa）
Chain g 1–110（110 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6J6N Cryo-EM structure of the yeast B*-b1 complex at an average resolution of 3.86 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 37 PDB declaration: 41-meric
Chain e 1–110（110 aa）
Chain g 1–110（110 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.86 Å
6J6Q Cryo-EM structure of the yeast B*-b2 complex at an average resolution of 3.7 angstrom Deposited 2019-01-15 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 38 PDB declaration: 42-meric
Chain e 1–110（110 aa）
Chain g 1–110（110 aa）
Not recorded IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 6 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6N7P S. cerevisiae spliceosomal E complex (UBC4) Deposited 2018-11-27 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain M 1–110（110 aa）
Not recorded ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6N7R Saccharomyces cerevisiae spliceosomal E complex (ACT1) Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain M 1–110（110 aa）
Not recorded ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
6N7X S. cerevisiae U1 snRNP Deposited 2018-11-28 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 15 PDB declaration: hexadecameric
Chain M 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
7B9V Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound Deposited 2020-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 45 PDB declaration: 50-meric
Chain j 1–110（110 aa）
Chain m 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 6 K POTASSIUM ION × 1 KGN D-chiro inositol hexakisphosphate × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 8 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.80 Å
7OQB The U2 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain u 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 9.00 Å
7OQC The U1 part of Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: octadecameric
Chain i 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.10 Å
7OQE Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1) Deposited 2021-06-03 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 36 PDB declaration: 39-meric
Chain i 1–110（110 aa）
Chain u 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.90 Å
8W2O Yeast U1 snRNP with humanized U1C Zinc-Finger domain Deposited 2024-02-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 16 PDB declaration: 18-meric
Chain M 1–110（110 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9；20 mM Hepes, pH7.9, 120 mM KCl, 2 mM EGTA
cryo-EM vitrification conditions Cryogen ETHANE；The grids were obtained with the chamber at 100% humidity, 2.5 s blotting time, -6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific)
Resolution 3.49 Å
9DTR Structure of the yeast post-catalytic P complex spliceosome at 2.3 Angstrom resolution Deposited 2024-10-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 42 PDB declaration: 47-meric
Chain j 1–110（110 aa）
Chain m 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 2 K POTASSIUM ION × 4 IHP INOSITOL HEXAKISPHOSPHATE × 2 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.31 Å

33 other PDB entries and 33 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name SMD2_YEAST
Isoform —
PDB entities 41
Chains and sequence ranges Author chain i； PDBConstruct 1–110； UniProt 1–110 Author chain m； PDBConstruct 1–110； UniProt 1–110 Author chain u； PDBConstruct 1–110； UniProt 1–110

U6 snRNA-associated Sm-like protein LSm4

OrganismNot specified

UniProt P40070

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain j； UniProt 1–187 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain h 1–187（187 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4C8Q Crystal structure of the yeast Lsm1-7-Pat1 complex Deposited 2013-10-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–114（114 aa） Fragment：RESIDUES 1-114
Not recorded CO COBALT (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM HEPES PH 7.0, 20% MPD, 10 MM HEXAMINE COBALT(III) CHLORIDE
Resolution 3.70 Å R-free 0.295
4C92 Crystal structure of the yeast Lsm1-7 complex Deposited 2013-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain D 1–114（114 aa） Fragment：RESIDUES 1-114
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM MES PH 6.0, 40% MPD
Resolution 2.30 Å R-free 0.259
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–93（93 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain N 1–93（93 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain N 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain G 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain N 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain N 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain G 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein–RNA Heteromer；Protein × 14 PDB declaration: hexadecameric
Chain G 1–93（93 aa）
Chain N 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain G 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain N 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain 4 1–187（187 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5VSU Structure of yeast U6 snRNP with 2'-phosphate terminated U6 RNA Deposited 2017-05-12 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain D 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；289 K；0.2 M NH4F 0.1 M HEPES pH 7.4 0.01 M MgCl2 18 % PEG 3,350
Resolution 3.10 Å R-free 0.298
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain s 1–187（187 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain s 1–187（187 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6ASO Structure of yeast U6 snRNP with 3'-phosphate terminated U6 RNA Deposited 2017-08-25 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain D 1–93（93 aa）
Not recorded K POTASSIUM ION × 2 MN MANGANESE (II) ION × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；280 mM sodium potassium tartrate 22.5 % glycerol 17.5 % PEG 3,350 1 mM manganese chloride
Resolution 2.71 Å R-free 0.247

13 other PDB entries and 19 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LSM4_YEAST
Isoform —
PDB entities 42
Chains and sequence ranges Author chain j； PDBConstruct 1–187； UniProt 1–187

U6 snRNA-associated Sm-like protein LSm5

OrganismNot specified

UniProt P40089

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain o； UniProt 1–93 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm6 × 1 （A6ZYX7） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
3JCM Cryo-EM structure of the spliceosomal U4/U6.U5 tri-snRNP Deposited 2015-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 30 PDB declaration: 34-meric
Chain f 1–93（93 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 M7M N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate) × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
4C8Q Crystal structure of the yeast Lsm1-7-Pat1 complex Deposited 2013-10-01 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–93（93 aa） Fragment：RESIDUES 1-93
Not recorded CO COBALT (II) ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM HEPES PH 7.0, 20% MPD, 10 MM HEXAMINE COBALT(III) CHLORIDE
Resolution 3.70 Å R-free 0.295
4C92 Crystal structure of the yeast Lsm1-7 complex Deposited 2013-10-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–93（93 aa） Fragment：RESIDUES 1-93
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 100 MM MES PH 6.0, 40% MPD
Resolution 2.30 Å R-free 0.259
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–93（93 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) CL CHLORIDE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M75 Crystal structure of Lsm1-7 complex Deposited 2013-08-12 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain L 1–93（93 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM MES 6.5, 25% PEG600, 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.95 Å R-free 0.291
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M77 Crystal structure of Lsm2-8 complex, space group I212121 Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain L 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.2；291 K；20% PEG2000MME, 100mM Sodium Citrate 5.2, 10% 2-propanol, 50mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 3.11 Å R-free 0.267
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain E 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M78 Crystal structure of Lsm2-8 complex, space group P21 Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric
Chain L 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.79 Å R-free 0.294
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain L 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain E 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7A Crystal structure of Lsm2-8 complex bound to the 3' end sequence of U6 snRNA Deposited 2013-08-12 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 3 Protein–RNA Heteromer；Protein × 14 PDB declaration: hexadecameric
Chain E 1–93（93 aa）
Chain L 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.78 Å R-free 0.278
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain E 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
4M7D Crystal structure of Lsm2-8 complex bound to the RNA fragment CGUUU Deposited 2013-08-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 7 PDB declaration: octameric
Chain L 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；291 K；100mM Sodium cacodylate 6.5, 25% Jeffamine ED2001, 5mM MgCl2, 8mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K
Resolution 2.60 Å R-free 0.283
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain 5 1–93（93 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
5VSU Structure of yeast U6 snRNP with 2'-phosphate terminated U6 RNA Deposited 2017-05-12 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain E 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.4；289 K；0.2 M NH4F 0.1 M HEPES pH 7.4 0.01 M MgCl2 18 % PEG 3,350
Resolution 3.10 Å R-free 0.298
5ZWM Cryo-EM structure of the yeast pre-B complex at an average resolution of 3.4~4.6 angstrom (tri-snRNP and U2 snRNP Part) Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain t 1–93（93 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ZN ZINC ION × 6 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
5ZWO Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom Deposited 2018-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain t 1–93（93 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.90 Å
6ASO Structure of yeast U6 snRNP with 3'-phosphate terminated U6 RNA Deposited 2017-08-25 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain E 1–93（93 aa）
Not recorded K POTASSIUM ION × 2 MN MANGANESE (II) ION × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；280 mM sodium potassium tartrate 22.5 % glycerol 17.5 % PEG 3,350 1 mM manganese chloride
Resolution 2.71 Å R-free 0.247

13 other PDB entries and 19 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LSM5_YEAST
Isoform —
PDB entities 43
Chains and sequence ranges Author chain o； PDBConstruct 1–93； UniProt 1–93

U6 snRNA-associated Sm-like protein LSm6

OrganismNot specified

UniProt A6ZYX7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 53 RNA 5 PDB declaration: 58-meric（58） Consistent with all polymer counts Chain z； UniProt 1–86 Not recorded U2 snRNA × 1 U6 snRNA-associated Sm-like protein LSm3 × 1 （P57743） U4 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 U6 snRNA-associated Sm-like protein LSm7 × 1 （P53905） U6 snRNA-associated Sm-like protein LSm8 × 1 （P47093） Pre-mRNA-splicing factor 8 × 1 （P33334） Pre-mRNA-splicing helicase BRR2 × 1 （P32639） Pre-mRNA-splicing factor SNU114 × 1 （P36048） Spliceosomal protein DIB1 × 1 （Q06819） 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12420） Pre-mRNA-processing factor 31 × 1 （P49704） U4/U6 small nuclear ribonucleoprotein PRP3 × 1 （Q03338） U4/U6 small nuclear ribonucleoprotein PRP4 × 1 （P20053） Yeast UBC4 gene for ubiquitin-conjugating enzyme × 1 Pre-mRNA-splicing factor 6 × 1 （P19735） 13 kDa ribonucleoprotein-associated protein × 1 （P39990） 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component × 1 （Q12368） Pre-mRNA-splicing factor 38 × 1 （Q00723） Pre-mRNA-splicing factor SPP381 × 1 （P38282） U2 snRNP component HSH155 × 1 （P49955） Pre-mRNA-splicing factor RSE1 × 1 （Q04693） Cold sensitive U2 snRNA suppressor 1 × 1 （Q02554） Protein HSH49 × 1 （Q99181） Pre-mRNA-splicing factor RDS3 × 1 （Q06835） Pre-mRNA-splicing factor PRP9 × 1 （P19736） Pre-mRNA-splicing factor PRP11 × 1 （Q07350） Pre-mRNA-splicing factor PRP21 × 1 （P32524） ;U2 small nuclear ribonucleoprotein A' ; × 1 （Q08963） Unknown × 1 ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P40567） RDS3 complex subunit 10 × 1 （P0C074） U6 snRNA-associated Sm-like protein LSm2 × 1 （P38203） Small nuclear ribonucleoprotein-associated protein B × 3 （P40018） Small nuclear ribonucleoprotein Sm D3 × 3 （P43321） Small nuclear ribonucleoprotein E × 3 （Q12330） Small nuclear ribonucleoprotein F × 3 （P54999） Small nuclear ribonucleoprotein G × 3 （P40204） Small nuclear ribonucleoprotein Sm D1 × 3 （Q02260） Small nuclear ribonucleoprotein Sm D2 × 3 （Q06217） U6 snRNA-associated Sm-like protein LSm4 × 1 （P40070） U6 snRNA-associated Sm-like protein LSm5 × 1 （P40089） GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ZN ZINC ION × 7 ELECTRON MICROSCOPY cryo-EM buffer：pH 7.9；Buffer pH: HEPES, 7.9; EDTA, 8.0 cryo-EM vitrification conditions：Cryogen ETHANE；Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity. Resolution 7.20 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 5NRL Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
5GAN The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom Deposited 2015-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 32 PDB declaration: 35-meric
Chain 6 1–86（86 aa）
Not recorded GTP GUANOSINE-5'-TRIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.9
Resolution 3.70 Å
6ASO Structure of yeast U6 snRNP with 3'-phosphate terminated U6 RNA Deposited 2017-08-25 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 8 PDB declaration: nonameric
Chain F 1–86（86 aa）
Not recorded K POTASSIUM ION × 2 MN MANGANESE (II) ION × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；289 K；280 mM sodium potassium tartrate 22.5 % glycerol 17.5 % PEG 3,350 1 mM manganese chloride
Resolution 2.71 Å R-free 0.247

2 other PDB entries and 2 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name LSM6_YEAS7
Isoform —
PDB entities 44
Chains and sequence ranges Author chain z； PDBConstruct 1–86； UniProt 1–86

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 5nrl

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 5nrl
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2. Structure Basics 2. Structure Basics

Entry ID entry_id5nrl
Deposition date deposition_date2017-04-24
Structure title titleStructure of a pre-catalytic spliceosome
Keywords keywordsSpliceosome, Splicing, Ribonucleoprotein; SPLICING
Experimental Method methodELECTRON MICROSCOPY
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier— Å
Radius of gyration Rg (electron density) rg_electron105.50 Å
Forward intensity I(0) i043981200000.00
Molecular weight molecular_weight1697200.0 kDa
Excluded volume excluded_volume2084500 ų
Envelope volume envelope_volume3760200 ų
Hydration-shell volume shell_volume297380 ų
Envelope diameter envelope_diameter362.8 Å
Shell Rg shell_rg102.90 Å
Envelope Rg envelope_rg101.60 Å
Shape Rg shape_rg105.50 Å
Total Rg total_rg105.60 Å
Total atoms total_atoms118696
Residues n_residues13938
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax277.3 Å
Rg (real space) rg_real101.40 Å
Rg uncertainty (real space) rg_real_error0.84 Å
I(0) (real space) i0_real4.2100e+10
I(0) uncertainty (real space) i0_real_error8.5750e+08
Rg (reciprocal space) rg_reciprocal104.10 Å
I(0) (reciprocal space) i0_reciprocal43720000000.0000
Solution quality estimate total_estimate0.9112
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary129.4 Å
Skewness Skewness skewness0.139
Kurtosis Kurtosis kurtosis-0.682
Angular range angular_range— – 0.0750 Å−1
Current regularization parameter α current_alpha0.8393
Highest regularization parameter α highest_alpha1353000000.0000
Real-space data points n_real_points16
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.996; Stabil: 0.961; Sysdev: 1.000; Positv: 1.000; Valcen: 0.973; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (46)

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8. Citations (1)

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9. Files and Curves (10)