5gam

Foot region of the yeast spliceosomal U4/U6.U5 tri-snRNP

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Pre-mRNA-splicing factor 8

OrganismNot specified

UniProt P33334

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 10 RNA 2 U5 snRNA × 1 Pre-mRNA-splicing factor SNU114 × 1 (P36048) ;U6 snRNA, 5' end ; × 1 Small nuclear ribonucleoprotein-associated protein B × 1 (P40018) Small nuclear ribonucleoprotein E × 1 (Q12330) Small nuclear ribonucleoprotein F × 1 (P54999) Small nuclear ribonucleoprotein G × 1 (P40204) Unknown polypeptide × 1 Small nuclear ribonucleoprotein Sm D3 × 1 (P43321) Small nuclear ribonucleoprotein Sm D1 × 1 (Q02260) Small nuclear ribonucleoprotein Sm D2 × 1 (Q06217) GUANOSINE-5'-TRIPHOSPHATE × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name PRP8_YEAST
Isoform —
PDB entities 2
Chains and sequence ranges Author chain A; PDBConstruct 1–735; UniProt 1–735

Pre-mRNA-splicing factor SNU114

OrganismNot specified

UniProt P36048

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 10 RNA 2 U5 snRNA × 1 Pre-mRNA-splicing factor 8 × 1 (P33334) ;U6 snRNA, 5' end ; × 1 Small nuclear ribonucleoprotein-associated protein B × 1 (P40018) Small nuclear ribonucleoprotein E × 1 (Q12330) Small nuclear ribonucleoprotein F × 1 (P54999) Small nuclear ribonucleoprotein G × 1 (P40204) Unknown polypeptide × 1 Small nuclear ribonucleoprotein Sm D3 × 1 (P43321) Small nuclear ribonucleoprotein Sm D1 × 1 (Q02260) Small nuclear ribonucleoprotein Sm D2 × 1 (Q06217) GUANOSINE-5'-TRIPHOSPHATE × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name SN114_YEAST
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C; PDBConstruct 1–1008; UniProt 1–1008

Small nuclear ribonucleoprotein-associated protein B

OrganismNot specified

UniProt P40018

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 10 RNA 2 U5 snRNA × 1 Pre-mRNA-splicing factor 8 × 1 (P33334) Pre-mRNA-splicing factor SNU114 × 1 (P36048) ;U6 snRNA, 5' end ; × 1 Small nuclear ribonucleoprotein E × 1 (Q12330) Small nuclear ribonucleoprotein F × 1 (P54999) Small nuclear ribonucleoprotein G × 1 (P40204) Unknown polypeptide × 1 Small nuclear ribonucleoprotein Sm D3 × 1 (P43321) Small nuclear ribonucleoprotein Sm D1 × 1 (Q02260) Small nuclear ribonucleoprotein Sm D2 × 1 (Q06217) GUANOSINE-5'-TRIPHOSPHATE × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RSMB_YEAST
Isoform —
PDB entities 5
Chains and sequence ranges Author chain b; PDBConstruct 1–196; UniProt 1–196

Small nuclear ribonucleoprotein E

OrganismNot specified

UniProt Q12330

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 10 RNA 2 U5 snRNA × 1 Pre-mRNA-splicing factor 8 × 1 (P33334) Pre-mRNA-splicing factor SNU114 × 1 (P36048) ;U6 snRNA, 5' end ; × 1 Small nuclear ribonucleoprotein-associated protein B × 1 (P40018) Small nuclear ribonucleoprotein F × 1 (P54999) Small nuclear ribonucleoprotein G × 1 (P40204) Unknown polypeptide × 1 Small nuclear ribonucleoprotein Sm D3 × 1 (P43321) Small nuclear ribonucleoprotein Sm D1 × 1 (Q02260) Small nuclear ribonucleoprotein Sm D2 × 1 (Q06217) GUANOSINE-5'-TRIPHOSPHATE × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RUXE_YEAST
Isoform —
PDB entities 6
Chains and sequence ranges Author chain e; PDBConstruct 1–94; UniProt 1–94

Small nuclear ribonucleoprotein F

OrganismNot specified

UniProt P54999

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 10 RNA 2 U5 snRNA × 1 Pre-mRNA-splicing factor 8 × 1 (P33334) Pre-mRNA-splicing factor SNU114 × 1 (P36048) ;U6 snRNA, 5' end ; × 1 Small nuclear ribonucleoprotein-associated protein B × 1 (P40018) Small nuclear ribonucleoprotein E × 1 (Q12330) Small nuclear ribonucleoprotein G × 1 (P40204) Unknown polypeptide × 1 Small nuclear ribonucleoprotein Sm D3 × 1 (P43321) Small nuclear ribonucleoprotein Sm D1 × 1 (Q02260) Small nuclear ribonucleoprotein Sm D2 × 1 (Q06217) GUANOSINE-5'-TRIPHOSPHATE × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RUXF_YEAST
Isoform —
PDB entities 7
Chains and sequence ranges Author chain f; PDBConstruct 1–86; UniProt 1–86

Small nuclear ribonucleoprotein G

OrganismNot specified

UniProt P40204

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 10 RNA 2 U5 snRNA × 1 Pre-mRNA-splicing factor 8 × 1 (P33334) Pre-mRNA-splicing factor SNU114 × 1 (P36048) ;U6 snRNA, 5' end ; × 1 Small nuclear ribonucleoprotein-associated protein B × 1 (P40018) Small nuclear ribonucleoprotein E × 1 (Q12330) Small nuclear ribonucleoprotein F × 1 (P54999) Unknown polypeptide × 1 Small nuclear ribonucleoprotein Sm D3 × 1 (P43321) Small nuclear ribonucleoprotein Sm D1 × 1 (Q02260) Small nuclear ribonucleoprotein Sm D2 × 1 (Q06217) GUANOSINE-5'-TRIPHOSPHATE × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name RUXG_YEAST
Isoform —
PDB entities 8
Chains and sequence ranges Author chain g; PDBConstruct 1–77; UniProt 1–77

Small nuclear ribonucleoprotein Sm D3

OrganismNot specified

UniProt P43321

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 10 RNA 2 U5 snRNA × 1 Pre-mRNA-splicing factor 8 × 1 (P33334) Pre-mRNA-splicing factor SNU114 × 1 (P36048) ;U6 snRNA, 5' end ; × 1 Small nuclear ribonucleoprotein-associated protein B × 1 (P40018) Small nuclear ribonucleoprotein E × 1 (Q12330) Small nuclear ribonucleoprotein F × 1 (P54999) Small nuclear ribonucleoprotein G × 1 (P40204) Unknown polypeptide × 1 Small nuclear ribonucleoprotein Sm D1 × 1 (Q02260) Small nuclear ribonucleoprotein Sm D2 × 1 (Q06217) GUANOSINE-5'-TRIPHOSPHATE × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name SMD3_YEAST
Isoform —
PDB entities 10
Chains and sequence ranges Author chain d; PDBConstruct 1–101; UniProt 1–101

Small nuclear ribonucleoprotein Sm D1

OrganismNot specified

UniProt Q02260

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 10 RNA 2 U5 snRNA × 1 Pre-mRNA-splicing factor 8 × 1 (P33334) Pre-mRNA-splicing factor SNU114 × 1 (P36048) ;U6 snRNA, 5' end ; × 1 Small nuclear ribonucleoprotein-associated protein B × 1 (P40018) Small nuclear ribonucleoprotein E × 1 (Q12330) Small nuclear ribonucleoprotein F × 1 (P54999) Small nuclear ribonucleoprotein G × 1 (P40204) Unknown polypeptide × 1 Small nuclear ribonucleoprotein Sm D3 × 1 (P43321) Small nuclear ribonucleoprotein Sm D2 × 1 (Q06217) GUANOSINE-5'-TRIPHOSPHATE × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name SMD1_YEAST
Isoform —
PDB entities 11
Chains and sequence ranges Author chain h; PDBConstruct 1–146; UniProt 1–146

Small nuclear ribonucleoprotein Sm D2

OrganismNot specified

UniProt Q06217

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein–RNA Heteromer Protein 10 RNA 2 U5 snRNA × 1 Pre-mRNA-splicing factor 8 × 1 (P33334) Pre-mRNA-splicing factor SNU114 × 1 (P36048) ;U6 snRNA, 5' end ; × 1 Small nuclear ribonucleoprotein-associated protein B × 1 (P40018) Small nuclear ribonucleoprotein E × 1 (Q12330) Small nuclear ribonucleoprotein F × 1 (P54999) Small nuclear ribonucleoprotein G × 1 (P40204) Unknown polypeptide × 1 Small nuclear ribonucleoprotein Sm D3 × 1 (P43321) Small nuclear ribonucleoprotein Sm D1 × 1 (Q02260) GUANOSINE-5'-TRIPHOSPHATE × 1 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name SMD2_YEAST
Isoform —
PDB entities 12
Chains and sequence ranges Author chain j; PDBConstruct 1–110; UniProt 1–110

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id5gam
Deposition date deposition_date2015-12-15
Structure title titleFoot region of the yeast spliceosomal U4/U6.U5 tri-snRNP
Keywords keywordsTranscription, pre-mRNA splicing, snRNP, GTPase, U5 snRNA, Prp8, spliceosome; TRANSCRIPTION
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5gam__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5gam__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

5gam__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)47.05 Å
Rg (electron density)46.94 Å
Total Rg47.15 Å
Atom count19123
Residues2186
Excluded volume331040 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 5gam__assembly_1__model_1 Dodecameric (12) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (13)

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6. Fold Classification (SCOP + CATH) 6 domains

CATH v4.4 (6 domains)

Domain ID domain_id5gamd00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily100 —
Domain ID domain_id5game00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily100 —
Domain ID domain_id5gamf00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily100 —
Domain ID domain_id5gamg00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily100 —
Domain ID domain_id5gamh00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily100 —
Domain ID domain_id5gamj00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily100 —
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7. Citations (2)