Current Protein Identity:P21698
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2N0Y NMR structure of the complex between the C-terminal domain of the Rift Valley fever virus protein NSs and the PH domain of the Tfb1 subunit of TFIIH Deposited 2015-03-18 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
247–265(19 aa)
Fragment:C-terminal domain residues247-265
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5;300 K;Ionic strength (raw mmCIF value) 0;Pressure ambient
NMR sample composition
1 mM [U-13C; U-15N] RNA polymerase II transcription factor B subunit 1, 2 mM Non-structural protein NS-S, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-13C; U-15N] RNA polymerase II transcription factor B subunit 1, 2 mM Non-structural protein NS-S, 100% D2O | 100% D2O
NMR sample composition
0.5 mM [U-15N] RNA polymerase II transcription factor B subunit 1, 1 mM Non-structural protein NS-S, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM RNA polymerase II transcription factor B subunit 1, 0.5 mM [U-13C; U-15N] Non-structural protein NS-S, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM RNA polymerase II transcription factor B subunit 1, 1 mM [U-13C; U-15N] Non-structural protein NS-S, 100% D2O | 100% D2O
NMR sample composition
0.4 mM RNA polymerase II transcription factor B subunit 1, 0.2 mM [U-15N] Non-structural protein NS-S, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 8T2L Crystal structure of LC3A in complex with the LIR of NSs4 Deposited 2023-06-06 | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
257–265(9 aa)
Fragment:GS is the remain of the removed expression tag. The N terminus of Nss has been fused with Q9H492 to facilitate the crystalization.
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;293 K;20% PEG3350, 0.2M Na-citrate, 5% Isopropanol, 0.1M MES buffer PH 6.5
|
Resolution 2.24 Å R-free 0.276 |
| 8T2L Crystal structure of LC3A in complex with the LIR of NSs4 Deposited 2023-06-06 | Assembly 2 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
257–265(9 aa)
Fragment:GS is the remain of the removed expression tag. The N terminus of Nss has been fused with Q9H492 to facilitate the crystalization.
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;293 K;20% PEG3350, 0.2M Na-citrate, 5% Isopropanol, 0.1M MES buffer PH 6.5
|
Resolution 2.24 Å R-free 0.276 |
| 8T2M Crystal structure of GABARAP in complex with the LIR of NSs4 Deposited 2023-06-06 | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
257–265(9 aa)
Fragment:;GS is the remain of the removed expression tag. The C terminus of Nss 257-265 has been fused with Q9H492 to facilitate the crystalization. The GG is a spacer between Nss and GABARAP protein.
;
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;293 K;0.1 M sodium acetate:HCl, pH 4.6, 8% PEG 4000
|
Resolution 1.27 Å R-free 0.178 |
| 8T2N Crystal structure of GABARAP in complex with the LIR of NSs3 Deposited 2023-06-06 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
234–255(22 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;293 K;0.2 M Potassium acetate, 20% PEG 3350
|
Resolution 1.88 Å R-free 0.264 |
| 8T2N Crystal structure of GABARAP in complex with the LIR of NSs3 Deposited 2023-06-06 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain D
234–255(22 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;293 K;0.2 M Potassium acetate, 20% PEG 3350
|
Resolution 1.88 Å R-free 0.264 |