Current Protein Identity：P27150
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AD2 RIBOSOMAL PROTEIN L1 MUTANT WITH SERINE 179 REPLACED BY CYSTEINE Deposited 1997-02-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–228（228 aa）
|
Mutation：S179C | SO4 SULFATE ION × 3 HG MERCURY (II) ION × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
DROPS CONTAINING: APPR. 15 MG/ML L1 50 MM GLYCINE, INITIALLY AT PH 10 5% (V/V) METHANE PENTANEDIOL 1.2 M AMMONIUM SULFATE EQUILIBRATED AGAINST 2.4 M AMMONIUM SULFATE 7% (V/V) METHANE PENTANEDIOL
|
Resolution 1.90 Å R-free 0.270 |
| 1EG0 FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME Deposited 2000-02-11 | Assembly 1 Protein–RNA Heteromer；Protein × 11 PDB declaration: pentadecameric（15） Consistent with all polymers |
Chain N
1–229（229 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.6；20 mM Hepes, 6 mM Mg(CH3COO)2 150 mM NH4Cl, 4 mM
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 11.50 Å |
| 1ZHO The structure of a ribosomal protein L1 in complex with mRNA Deposited 2005-04-26 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
1–228（228 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；KCl, MgCl2, HgCl2, PEG 10000, cacodylate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.60 Å R-free 0.281 |
| 1ZHO The structure of a ribosomal protein L1 in complex with mRNA Deposited 2005-04-26 | Assembly 2 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain C
1–228（228 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；KCl, MgCl2, HgCl2, PEG 10000, cacodylate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.60 Å R-free 0.281 |
| 1ZHO The structure of a ribosomal protein L1 in complex with mRNA Deposited 2005-04-26 | Assembly 3 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain E
1–228（228 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；KCl, MgCl2, HgCl2, PEG 10000, cacodylate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.60 Å R-free 0.281 |
| 1ZHO The structure of a ribosomal protein L1 in complex with mRNA Deposited 2005-04-26 | Assembly 4 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain G
1–228（228 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；KCl, MgCl2, HgCl2, PEG 10000, cacodylate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.60 Å R-free 0.281 |
| 2HW8 Structure of ribosomal protein L1-mRNA complex at 2.1 resolution. Deposited 2006-08-01 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
1–228（228 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | MG MAGNESIUM ION × 1 K POTASSIUM ION × 1 BU1 1,4-BUTANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG1000, sodium cacodylate,0.5% glycerol, 0.2 mM HgCl2 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.10 Å R-free 0.247 |
| 2OUM The first domain of L1 from Thermus thermophilus Deposited 2007-02-12 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–68（67 aa）
Chain A
160–229（70 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；277 K；30% v/v polyethylenglycol 4K, 100 mM Tris-HCl, 200 mM acetate Na, pH 8.5, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.55 Å R-free 0.270 |
| 2OV7 The first domain of the ribosomal protein L1 from Thermus thermophilus Deposited 2007-02-13 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
2–68（67 aa）
Chain A
160–229（70 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；277 K；30% v/v polyethylenglycol 4K, 100 mM Tris-HCl, 200 mM LiSO4, pH 8.5, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.30 Å R-free 0.233 |
| 2OV7 The first domain of the ribosomal protein L1 from Thermus thermophilus Deposited 2007-02-13 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
2–68（67 aa）
Chain B
160–229（70 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；277 K；30% v/v polyethylenglycol 4K, 100 mM Tris-HCl, 200 mM LiSO4, pH 8.5, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.30 Å R-free 0.233 |
| 2OV7 The first domain of the ribosomal protein L1 from Thermus thermophilus Deposited 2007-02-13 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
2–68（67 aa）
Chain C
160–229（70 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；277 K；30% v/v polyethylenglycol 4K, 100 mM Tris-HCl, 200 mM LiSO4, pH 8.5, VAPOR DIFFUSION, temperature 277K
|
Resolution 2.30 Å R-free 0.233 |
| 2VPL The structure of the complex between the first domain of L1 protein from Thermus thermophilus and mRNA from Methanococcus jannaschii Deposited 2008-03-01 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
2–68（67 aa）
Fragment：FIRST DOMAIN, RESIDUES 2-68,160-229
Chain A
160–229（70 aa）
Fragment：FIRST DOMAIN, RESIDUES 2-68,160-229
|
Not recorded | K POTASSIUM ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.274 |
| 2VPL The structure of the complex between the first domain of L1 protein from Thermus thermophilus and mRNA from Methanococcus jannaschii Deposited 2008-03-01 | Assembly 2 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain C
2–68（67 aa）
Fragment：FIRST DOMAIN, RESIDUES 2-68,160-229
Chain C
160–229（70 aa）
Fragment：FIRST DOMAIN, RESIDUES 2-68,160-229
|
Not recorded | K POTASSIUM ION × 1 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.274 |
| 3TG8 Mutant ribosomal protein L1 lacking ala158 from thermus thermophilus Deposited 2011-08-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–229（229 aa）
|
Mutation：lacking A158 | PG4 TETRAETHYLENE GLYCOL × 3 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；30 mM Tris-HCl, 75 mM NaCl with of 15% PEG 4K, 50 mM Tris-HCl,
0.2 M KBr (# 10 of Clear Strategy Screen I (MDL)) and 14% 2-methyl-2,4-pentandiol, 0.25 M MgCl2 in a 4:1:1, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.5
|
Resolution 1.95 Å R-free 0.226 |
| 3U4M Crystal structure of ribosomal protein tthl1 in complex with 80nt 23s rna from thermus thermophilus Deposited 2011-10-10 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
1–229（229 aa）
|
Mutation：V218R | SO4 SULFATE ION × 2 MLI MALONATE ION × 7 MG MAGNESIUM ION × 5 CL CHLORIDE ION × 2 NA SODIUM ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；295 K；50mM NaCl, 2.5M Ammonium sulfate, 50mM MES, 10mM Magnesium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.00 Å R-free 0.208 |
| 3U56 Crystal structure of mutant ribosomal protein T217V TthL1 in complex with 80nt 23S RNA from Thermus thermophilus Deposited 2011-10-11 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
1–229（229 aa）
|
Mutation：V218R | MG MAGNESIUM ION × 11 MLI MALONATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；295 K；50mM NaCl, 2.5M Ammonium sulfate, 50mM MES, 10mM Magnesium acetate , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.10 Å R-free 0.238 |
| 3UMY Crystal structure of mutant ribosomal protein T217A TthL1 in complex with 80nt 23S RNA from Thermus thermophilus Deposited 2011-11-15 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
2–229（228 aa）
|
Mutation：T217A | CL CHLORIDE ION × 2 SO4 SULFATE ION × 5 NA SODIUM ION × 1 MG MAGNESIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；295 K；2.0 M Ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.90 Å R-free 0.246 |
| 487D SEVEN RIBOSOMAL PROTEINS FITTED TO A CRYO-ELECTRON MICROSCOPIC MAP OF THE LARGE 50S SUBUNIT AT 7.5 ANGSTROMS RESOLUTION Deposited 2000-02-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain H
6–229（224 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY | mmCIF provides none of the parsed conditions | Resolution 7.50 Å |
| 4F9T Ribosomal protein L1 from Thermus thermophilus with substitution Thr217Ala Deposited 2012-05-21 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–229（229 aa）
|
Mutation：T217A | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 SO4 SULFATE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 10；295 K；15 MG/ML L1 50 MM GLYCINE,INITIALLY AT PH 10 5% (V/V)METHANE PENTANEDIOL 1.2 M AMMONIUM SULFATE EQUILIBRATED AGAINST 2.4 M AMMONIUM SULFATE 7% (V/V) METHANE PENTANEDIOL , VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.46 Å R-free 0.202 |
| 4QG3 Crystal structure of mutant ribosomal protein G219V TthL1 in complex with 80nt 23S RNA from Thermus thermophilus Deposited 2014-05-22 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
2–229（228 aa）
|
Mutation：G219V | SO4 SULFATE ION × 5 IPA ISOPROPYL ALCOHOL × 8 BME BETA-MERCAPTOETHANOL × 4 URE UREA × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；1.8 M Ammonium sulfate, 0.05 M Bis-Tris,pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.00 Å R-free 0.226 |
| 4QGB Crystal structure of mutant ribosomal protein G219V TthL1 Deposited 2014-05-22 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
14–229（216 aa）
|
Mutation：G219V | CL CHLORIDE ION × 1 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；2 M ammonium sulfate, 0.1 M sodium acetate, 0.5 l 0.65% polyacrylic acid 5100 sodium salt, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.251 |
| 4QGB Crystal structure of mutant ribosomal protein G219V TthL1 Deposited 2014-05-22 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
14–229（216 aa）
|
Mutation：G219V | CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；2 M ammonium sulfate, 0.1 M sodium acetate, 0.5 l 0.65% polyacrylic acid 5100 sodium salt, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.251 |
| 4QGB Crystal structure of mutant ribosomal protein G219V TthL1 Deposited 2014-05-22 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
14–229（216 aa）
Chain B
14–229（216 aa）
|
Mutation：G219V Mutation：G219V | CL CHLORIDE ION × 3 ACT ACETATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.6；293 K；2 M ammonium sulfate, 0.1 M sodium acetate, 0.5 l 0.65% polyacrylic acid 5100 sodium salt, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.251 |
| 4QVI Crystal structure of mutant ribosomal protein M218L TthL1 in complex with 80nt 23S RNA from Thermus thermophilus Deposited 2014-07-15 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Review required |
Chain A
1–229（229 aa）
|
Mutation：M218L | SO4 SULFATE ION × 4 ACT ACETATE ION × 4 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；2.5 M ammonium sulfate, 0.05M MES , 0.01M Magnesium Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.90 Å R-free 0.217 |
| 4REO Mutant ribosomal protein l1 from thermus thermophilus with threonine 217 replaced by valine Deposited 2014-09-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–229（229 aa）
|
Mutation：T217V | SO4 SULFATE ION × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 GLY GLYCINE × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 10；295 K；15 MG/ML L1 50 MM GLYCINE, INITIALLY AT PH 10 5% (V/V) METHANE PENTANEDIOL 1.2 M AMMONIUM SULFATE EQUILIBRATED AGAINST 2.4 M AMMONIUM SULFATE 7% (V/V) METHANE PENTANEDIOL, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.35 Å R-free 0.209 |
| 4V42 Crystal structure of the ribosome at 5.5 A resolution. Deposited 2001-03-30 | Assembly 1 Insufficient information Heteromer；Protein × 42 PDB declaration: 49-meric（49） Consistent with all polymers |
Chain BC
1–228（228 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 5.50 Å |
| 4V4P Crystal structure of 70S ribosome with thrS operator and tRNAs. Deposited 2005-01-19 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric（58） Consistent with all polymers |
Chain AC
1–228（228 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 5.50 Å R-free 0.356 |
| 4V4X Crystal structure of the 70S Thermus thermophilus ribosome showing how the 16S 3'-end mimicks mRNA E and P codons. Deposited 2006-06-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain BC
0–228（229 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 5.00 Å R-free 0.323 |
| 4V4Y Crystal structure of the 70S Thermus thermophilus ribosome with translocated and rotated Shine-Dalgarno Duplex. Deposited 2006-06-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain BC
0–228（229 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 5.50 Å R-free 0.326 |
| 4V4Z 70S Thermus thermophilous ribosome functional complex with mRNA and E- and P-site tRNAs at 4.5A. Deposited 2006-06-27 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain BC
0–228（229 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 4.51 Å R-free 0.345 |
| 4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 708 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.20 Å R-free 0.238 |
| 4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 79
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 673 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.20 Å R-free 0.238 |
| 4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 699 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.10 Å R-free 0.193 |
| 4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 79
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 646 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.10 Å R-free 0.193 |
| 4WZO Complex of 70S ribosome with tRNA-fMet and mRNA Deposited 2014-11-20 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 56-MERIC（56） Review required |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 576 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
Resolution 3.30 Å R-free 0.258 |
| 5E7K Structure of T. thermophilus 70S ribosome complex with mRNA and cognate tRNALys in the A-site Deposited 2015-10-12 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 628 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.20 Å R-free 0.258 |
| 5E7K Structure of T. thermophilus 70S ribosome complex with mRNA and cognate tRNALys in the A-site Deposited 2015-10-12 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 79
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 510 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.20 Å R-free 0.258 |
| 5EL5 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position Deposited 2015-11-04 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC（55） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 660 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.254 |
| 5EL6 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position and antibiotic paromomycin Deposited 2015-11-04 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 593 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.10 Å R-free 0.249 |
| 5EL6 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position and antibiotic paromomycin Deposited 2015-11-04 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 79
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 480 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.10 Å R-free 0.249 |
| 5EL7 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position and antibiotic paromomycin Deposited 2015-11-04 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 687 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.247 |
| 5EL7 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position and antibiotic paromomycin Deposited 2015-11-04 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 79
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 574 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.15 Å R-free 0.247 |
| 5IB7 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet, near-cognate tRNALys with U-G mismatch in the A-site and antibiotic paromomycin Deposited 2016-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 695 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 2.99 Å R-free 0.241 |
| 5IB8 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNALys with U-G mismatch in the A-site Deposited 2016-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 723 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 3.13 Å R-free 0.244 |
| 5IBB Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAVal in the A-site Deposited 2016-02-22 | Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 736 SPE THERMINE × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 2.96 Å R-free 0.243 |
| 5IBB Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAVal in the A-site Deposited 2016-02-22 | Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric（55） Consistent with all polymers |
Chain 79
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 608 SPE THERMINE × 4 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
Resolution 2.96 Å R-free 0.243 |
| 5IMQ Structure of ribosome bound to cofactor at 3.8 angstrom resolution Deposited 2016-03-06 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Z
1–229（229 aa）
|
Not recorded | GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Buffer G
5 mM HEPES pH 7.5, 10 mM MgAc, 50 mM KCl, 10 mM NH4Cl, and 6 mM 2-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 5IMR Structure of ribosome bound to cofactor at 5.7 angstrom resolution Deposited 2016-03-06 | Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain Z
1–229（229 aa）
|
Not recorded | GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5；Buffer G
5 mM HEPES pH 7.5, 10 mM MgAc, 50 mM KCl, 10 mM NH4Cl, and 6 mM 2-mercaptoethanol
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å |
| 5J8B Crystal structure of Elongation Factor 4 (EF-4/LepA) in complex with GDPCP bound to the Thermus thermophilus 70S ribosome Deposited 2016-04-07 | Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric（59） Consistent with all polymers |
Chain C
2–229（228 aa）
|
Not recorded | MG MAGNESIUM ION × 919 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
|
Resolution 2.60 Å R-free 0.261 |
| 5NPM CRYSTAL STRUCTURE OF MUTANT RIBOSOMAL PROTEIN TTHL1 LACKING 8 N-TERMINAL RESIDUES IN COMPLEX WITH 80NT 23S RNA FROM THERMUS THERMOPHILUS Deposited 2017-04-17 | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric（2） Consistent with all polymers |
Chain A
10–229（220 aa）
|
Not recorded | MLI MALONATE ION × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；295 K；2.4 M sodium malonate
|
Resolution 2.70 Å R-free 0.249 |
| 5ZLU Ribosome Structure bound to ABC-F protein. Deposited 2018-03-29 | Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain Y
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6GSL Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAArg in the A-site Deposited 2018-06-14 | Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric（60） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 954 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
|
Resolution 3.16 Å |
| 6GSL Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAArg in the A-site Deposited 2018-06-14 | Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric（54） Consistent with all polymers |
Chain 79
1–229（229 aa）
|
Not recorded | MG MAGNESIUM ION × 810 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
|
Resolution 3.16 Å |
| 6QNQ 70S ribosome initiation complex (IC) with experimentally assigned potassium ions Deposited 2019-02-11 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | K POTASSIUM ION × 169 MG MAGNESIUM ION × 543 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20K, PEG-550mme
|
Resolution 3.50 Å R-free 0.251 |
| 6QNR 70S ribosome elongation complex (EC) with experimentally assigned potassium ions Deposited 2019-02-11 | Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric（57） Consistent with all polymers |
Chain 71
1–229（229 aa）
|
Not recorded | K POTASSIUM ION × 211 MG MAGNESIUM ION × 585 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20k, PEG-550mme
|
Resolution 3.10 Å R-free 0.253 |
| 6QNR 70S ribosome elongation complex (EC) with experimentally assigned potassium ions Deposited 2019-02-11 | Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric（56） Consistent with all polymers |
Chain 79
1–229（229 aa）
|
Not recorded | K POTASSIUM ION × 155 MG MAGNESIUM ION × 409 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20k, PEG-550mme
|
Resolution 3.10 Å R-free 0.253 |