Current Protein Identity：P27540
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1X0O human ARNT C-terminal PAS domain Deposited 2005-03-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
356–470（115 aa）
Fragment：C-TERMINAL PAS DOMAIN
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 17mM NaCl；Pressure ambient
NMR measurement conditions
pH 6.6；310 K；Ionic strength (raw mmCIF value) 17mM NaCl；Pressure ambient
NMR sample composition
1mM hARNT C-term PAS Domain U-15N,13C; 50mM Tris buffer; 17mM NaCl, 5mM DTT | 90% H2O/10% D2O
NMR sample composition
0.478mM hARNT C-term PAS Domain U-15N; 50mM Tris buffer; 17mM NaCl, 5mM DTT | 93% H2O/7% D2O
|
Resolution not provided |
| 2A24 HADDOCK Structure of HIF-2a/ARNT PAS-B Heterodimer Deposited 2005-06-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
358–465（108 aa）
Fragment：C-terminal PAS domain (PAS-B)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；Ionic strength (raw mmCIF value) 50mM Tris, 17mM NaCl, 5mM DTT
NMR sample composition
250 micro M (15N)HIF-2alpha PAS-B (240-350) + 1mM ARNT PAS-B (356-470), 50mM Tris (pH=7.5), 17mM NaCl, 5mM DTT
NMR sample composition
250 micro M (15N)ARNT PAS-B (356-470) + 1mM HIF-2alpha PAS-B (240-350), 50mM Tris (pH=7.5), 17mM NaCl, 5mM DTT
|
Resolution not provided |
| 2B02 Crystal Structure of ARNT PAS-B Domain Deposited 2005-09-12 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
354–470（117 aa）
Fragment：PAS-B Domain
|
Mutation：C358S Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.65；295 K；Ammonium sulfate, PEG 400, HEPES, pH 8.65, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.50 Å R-free 0.208 |
| 2HV1 HADDOCK structure of ARNT PAS-B Homodimer Deposited 2006-07-27 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
356–470（115 aa）
Fragment：C-terminal PAS domain (PAS-B), residues 356-470
Chain B
356–470（115 aa）
Fragment：C-terminal PAS domain (PAS-B), residues 356-470
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；298 K；Ionic strength (raw mmCIF value) 50mM Tris, 17mM NaCl
NMR sample composition
1mM down to 125 micro M (15N) ARNT PAS-B, 50mM Tris, 17mM NaCl, 5mM DTT
NMR sample composition
75 micro M (15N) ARNT PAS-B, 400 micro M ARNT PAS-B with CMSTL spin label
attached (to C358 or C451), 50mM Tris, 17mM NaCl
|
Resolution not provided |
| 2K7S Human ARNT C-Terminal PAS Domain, 3 Residue IB slip Deposited 2008-08-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
356–470（115 aa）
Fragment：ARNT PAS-B
|
Mutation：F444Q, F446A, Y456T | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.5；298 K；Ionic strength (raw mmCIF value) 17；Pressure ambient
NMR sample composition
900 uM [U-98% 13C; U-98% 15N] ARNT PAS-B Slipped IB Strand, 50 mM TRIS, 17 mM sodium chloride, 5 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 3F1N Crystal structure of a high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains, with internally bound ethylene glycol. Deposited 2008-10-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：ARNT C-terminal PAS domain
|
Mutation：E362R | EDO 1,2-ETHANEDIOL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
vapor diffusion combined with microseeding；pH 6.5；293 K；30% PEG 3350, 0.1M BisTris, 0.05M Tris, 0.017M NaCl, 0.005M DTT, pH 6.5, vapor diffusion combined with microseeding, temperature 293K
|
Resolution 1.48 Å R-free 0.199 |
| 3F1O Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains, with an internally-bound artificial ligand Deposited 2008-10-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：ARNT C-terminal PAS domain
|
Mutation：E362R | 2XY N-[2-nitro-4-(trifluoromethyl)phenyl]morpholin-4-amine × 1 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
vapor diffusion combined with microseeding；pH 6.5；293 K；30% PEG 3350, 0.1M BisTris, 0.05M Tris, 0.017M NaCl, 0.005M DTT , pH 6.5, vapor diffusion combined with microseeding, temperature 293K
|
Resolution 1.60 Å R-free 0.203 |
| 3F1P Crystal structure of a high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains Deposited 2008-10-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：ARNT C-terminal PAS domain
|
Mutation：E362R | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
vapor diffusion combined with microseeding；pH 6.5；293 K；30% PEG3350, 0.1M BisTris, 0.05M Tris, 0.017M NaCl, 0.005M DTT, pH 6.5, vapor diffusion combined with microseeding, temperature 293K
|
Resolution 1.17 Å R-free 0.167 |
| 3H7W Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains with the artificial ligand THS017 Deposited 2009-04-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：ARNT C-terminal PAS domain (UNP residues 356 to 470)
|
Mutation：E362R | 018 2-nitro-N-(thiophen-3-ylmethyl)-4-(trifluoromethyl)aniline × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；0.1M BisTris, 17% PEG3350, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.65 Å R-free 0.236 |
| 3H82 Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains with the artificial ligand THS020 Deposited 2009-04-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：ARNT C-terminal PAS domain (UNP residues 356 to 470)
|
Mutation：E362R | 020 N-(furan-2-ylmethyl)-2-nitro-4-(trifluoromethyl)aniline × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；0.1M BisTris, 25% PEG3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.50 Å R-free 0.234 |
| 4EQ1 Crystal Structure of the ARNT PAS-B homodimer Deposited 2012-04-17 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
357–464（108 aa）
Fragment：PAS-B domain (UNP residues 357-464)
Chain B
357–464（108 aa）
Fragment：PAS-B domain (UNP residues 357-464)
|
Not recorded | PE5 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；2M Ammonium Sulfate, 3% PEG400, 1% PEI, 50mM Tris pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.60 Å R-free 0.227 |
| 4GHI Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains in complex with a benzoxadiazole antagonist Deposited 2012-08-07 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：C-terminal PAS domain, UNP residues 356-470
|
Mutation：E362R | 0X3 N-(3-chloro-5-fluorophenyl)-4-nitro-2,1,3-benzoxadiazol-5-amine × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6；293 K；20% PEG400
50 mM BisTris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.50 Å R-free 0.196 |
| 4GS9 Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains in complex with an inactive benzoxadiazole antagonist Deposited 2012-08-27 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：ARNT PAS-B domain, UNP residues 356-470
|
Mutation：E362R | 0XB N-(3-fluorophenyl)-4-nitro-2,1,3-benzoxadiazol-5-amine × 1 PE8 3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6；293 K；20% PEG 3350
50 mM BISTRIS, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.72 Å R-free 0.202 |
| 4H6J Identification of Cys 255 in HIF-1 as a novel site for development of covalent inhibitors of HIF-1 /ARNT PasB domain protein-protein interaction. Deposited 2012-09-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
357–470（114 aa）
Fragment：PAS-B DOMAIN, UNP residues 357-470
|
Mutation：E362R | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；20% PEG 8000, 0.2M MgCl2, 0.1M Tris pH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.52 Å R-free 0.248 |
| 4LPZ ARNT transcription factor/coactivator complex Deposited 2013-07-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
356–470（115 aa）
Fragment：PAS 2 and PAC domain residues 356-470
Chain B
356–470（115 aa）
Fragment：PAS 2 and PAC domain residues 356-470
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；1M Succinic acid, 30% w/v D-Sorbitol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 3.15 Å R-free 0.272 |
| 4PKY ARNT/HIF transcription factor/coactivator complex Deposited 2014-05-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
342–456（115 aa）
Fragment：C-terminal PAS 2 domain residues 342-456
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；1.6 M magnesium sulfate, 0.1 M MES pH 6.5, 20% (w/v) ethylene glycol
|
Resolution 3.20 Å R-free 0.270 |
| 4PKY ARNT/HIF transcription factor/coactivator complex Deposited 2014-05-15 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain D
342–456（115 aa）
Fragment：C-terminal PAS 2 domain residues 342-456
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；293 K；1.6 M magnesium sulfate, 0.1 M MES pH 6.5, 20% (w/v) ethylene glycol
|
Resolution 3.20 Å R-free 0.270 |
| 4XT2 Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains in complex with a tetrazole-containing antagonist Deposited 2015-01-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
342–456（115 aa）
Fragment：C-terminal PAS domain (UNP residues 342-456)
|
Mutation：E362R | 43L (5S,7R)-5,7-bis(3-bromophenyl)-4,5,6,7-tetrahydrotetrazolo[1,5-a]pyrimidine × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293.15 K；2 uL 500 uM protein against 100 mM Bis-Tris, 25% PEG3350
|
Resolution 1.70 Å R-free 0.192 |
| 4XT2 Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains in complex with a tetrazole-containing antagonist Deposited 2015-01-22 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
342–456（115 aa）
Fragment：C-terminal PAS domain (UNP residues 342-456)
|
Mutation：E362R | 43L (5S,7R)-5,7-bis(3-bromophenyl)-4,5,6,7-tetrahydrotetrazolo[1,5-a]pyrimidine × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293.15 K；2 uL 500 uM protein against 100 mM Bis-Tris, 25% PEG3350
|
Resolution 1.70 Å R-free 0.192 |
| 5TBM Crystal structure of PT2385 bound to HIF2a-B*:ARNT-B* complex Deposited 2016-09-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
340–453（114 aa）
Fragment：UNP residues 340-453
|
Not recorded | 79A 3-{[(1S)-2,2-difluoro-1-hydroxy-7-(methylsulfonyl)-2,3-dihydro-1H-inden-4-yl]oxy}-5-fluorobenzonitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.4；293 K；50 mM Bis-Tris pH5.4, 16% PEG 3350. use freshly crushed crystal as seed.
|
Resolution 1.85 Å R-free 0.300 |
| 5TBM Crystal structure of PT2385 bound to HIF2a-B*:ARNT-B* complex Deposited 2016-09-12 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
340–453（114 aa）
Fragment：UNP residues 340-453
|
Not recorded | 79A 3-{[(1S)-2,2-difluoro-1-hydroxy-7-(methylsulfonyl)-2,3-dihydro-1H-inden-4-yl]oxy}-5-fluorobenzonitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 5.4；293 K；50 mM Bis-Tris pH5.4, 16% PEG 3350. use freshly crushed crystal as seed.
|
Resolution 1.85 Å R-free 0.300 |
| 5UFP Crystal structure of PT2399 bound to HIF2a-B*:ARNT-B* complex Deposited 2017-01-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
342–453（112 aa）
Fragment：UNP residues 342-453
|
Not recorded | 86D 3-({(1S)-7-[(difluoromethyl)sulfonyl]-2,2-difluoro-1-hydroxy-2,3-dihydro-1H-inden-4-yl}oxy)-5-fluorobenzonitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；complex of HIF2-B*:ARNT-B* 10-12 mg/mL in 10 mM Tris-HCl, pH8.0 and 50 mM KCl; Crystallization buffer of
50 mM Bis-Tris, pH5.4 and 16% PEG3350.
|
Resolution 1.90 Å R-free 0.271 |
| 5V0L Crystal structure of the AHR-ARNT heterodimer in complex with the DRE Deposited 2017-02-28 | Assembly 1 Protein–DNA Heteromer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
70–346（277 aa）
|
Not recorded | PEG DI(HYDROXYETHYL)ETHER × 2 CIT CITRIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；277 K；10%-12% PEG 20000, 4-6% Tacsimate pH 7.0 or 0.1 M Bis-Tris pH 6.5
|
Resolution 4.00 Å R-free 0.323 |
| 6CZW Crystal structure of PT1940 bound to HIF2a-B*:ARNT-B* complex Deposited 2018-04-09 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：residues 356-470
|
Mutation：E13R | FO7 {2-bromo-3-(3-chloro-5-fluorophenoxy)-6-[(difluoromethyl)sulfonyl]phenyl}methanol × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；50 mM Bis-Tris pH5.4, 16% PEG 3350. Seed with crushed crystal right after drops are set up
|
Resolution 1.60 Å R-free 0.251 |
| 6CZW Crystal structure of PT1940 bound to HIF2a-B*:ARNT-B* complex Deposited 2018-04-09 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：residues 356-470
|
Mutation：E13R | FO7 {2-bromo-3-(3-chloro-5-fluorophenoxy)-6-[(difluoromethyl)sulfonyl]phenyl}methanol × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；50 mM Bis-Tris pH5.4, 16% PEG 3350. Seed with crushed crystal right after drops are set up
|
Resolution 1.60 Å R-free 0.251 |
| 6D09 Crystal structure of PT2440 bound to HIF2a-B*:ARNT-B* complex Deposited 2018-04-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：residues 356-470
|
Mutation：E13R | FOJ 3-{[(3R)-4-(difluoromethyl)-2,2-difluoro-3-hydroxy-1,1-dioxo-2,3-dihydro-1H-1-benzothiophen-5-yl]oxy}-5-fluorobenzonitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；50 mM Bis-Tris pH5.4 and 16% PEG3350. Seeding with crushed crystals right after setup
|
Resolution 1.85 Å R-free 0.282 |
| 6D09 Crystal structure of PT2440 bound to HIF2a-B*:ARNT-B* complex Deposited 2018-04-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：residues 356-470
|
Mutation：E13R | FOJ 3-{[(3R)-4-(difluoromethyl)-2,2-difluoro-3-hydroxy-1,1-dioxo-2,3-dihydro-1H-1-benzothiophen-5-yl]oxy}-5-fluorobenzonitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；50 mM Bis-Tris pH5.4 and 16% PEG3350. Seeding with crushed crystals right after setup
|
Resolution 1.85 Å R-free 0.282 |
| 6D0B Crystal structure of PT1614 bound to HIF2a-B*:ARNT-B* complex Deposited 2018-04-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：residues 356-470
|
Mutation：E13R | FOV N-(3-chloro-5-fluorophenyl)-2-nitro-4-(trifluoromethanesulfonyl)aniline × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；Bis-Tris pH5.4, 16% PEG3350. Seeding right after setup using crushed crystals
|
Resolution 1.60 Å R-free 0.235 |
| 6D0B Crystal structure of PT1614 bound to HIF2a-B*:ARNT-B* complex Deposited 2018-04-10 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：residues 356-470
|
Mutation：E13R | FOV N-(3-chloro-5-fluorophenyl)-2-nitro-4-(trifluoromethanesulfonyl)aniline × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；Bis-Tris pH5.4, 16% PEG3350. Seeding right after setup using crushed crystals
|
Resolution 1.60 Å R-free 0.235 |
| 6D0C Crystal structure of HIF2a-B*:ARNT-B* complex Deposited 2018-04-10 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
Fragment：residues 356-470
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；Bis-Tris, pH5.4 and 16% PEG3350, Seeding with crushed crystals right after setup
|
Resolution 1.50 Å R-free 0.240 |
| 6X21 Crystal structure of PT1673 bound to HIF2a-B*:ARNT-B* complex Deposited 2020-05-19 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–467（112 aa）
|
Mutation：E362R | UKJ 1-(3-bromo-5-fluorophenoxy)-4-[(difluoromethyl)sulfonyl]-2-nitrobenzene × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；Bis-Tris, pH5.4, 16% PEG3350
|
Resolution 1.54 Å R-free 0.233 |
| 6X28 Crystal structure of PT2243 bound to HIF2a-B*:ARNT-B* complex Deposited 2020-05-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–467（112 aa）
|
Mutation：E362R | ULG (1R)-4-(3,5-difluorophenoxy)-7-(trifluoromethyl)-2,3-dihydro-1H-inden-1-ol × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；Bis-Tris, pH5.4, 16% PEG3350
|
Resolution 1.92 Å R-free 0.287 |
| 6X2H Crystal structure of PT2863 bound to HIF2a-B*:ARNT-B* complex Deposited 2020-05-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–467（112 aa）
|
Not recorded | ULD cis-3-({(1S)-7-[dihydroxy(trifluoromethyl)-lambda~4~-sulfanyl]-2,2-difluoro-1-hydroxy-2,3-dihydro-1H-inden-4-yl}oxy)cyclobutane-1-carbonitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；Bis-Tris pH5.4, 16% 3350
|
Resolution 2.00 Å R-free 0.258 |
| 6X37 Crystal structure of PT3245 bound to HIF2a-B*:ARNT-B* complex Deposited 2020-05-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–467（112 aa）
|
Mutation：E362R | ULS 3-fluoro-5-{[(7R)-7-hydroxy-1-(trifluoromethyl)-6,7-dihydro-5H-cyclopenta[c]pyridin-4-yl]oxy}benzonitrile × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；Bis-Tris, pH5.4, 16% PEG 3350
|
Resolution 1.94 Å R-free 0.282 |
| 6X3D Crystal structure of PT3388 bound to HIF2a-B*:ARNT-B* complex Deposited 2020-05-21 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
342–453（112 aa）
|
Mutation：E362R | ULM (6R,7S)-4-[(3,3-difluorocyclobutyl)oxy]-6-fluoro-1-(trifluoromethyl)-6,7-dihydro-5H-cyclopenta[c]pyridin-7-ol × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.4；293 K；Bis-Tris, pH5.4 and 16% PEG 3350
|
Resolution 2.00 Å R-free 0.277 |
| 8CK3 STRUCTURE OF HIF2A-ARNT HETERODIMER IN COMPLEX WITH (S)-1-(3,5-Difluoro-phenyl)-5,5-difluoro-3-methanesulfonyl-5,6-dihydro-4H-cyclopenta[c]thiophen-4-ol Deposited 2023-02-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
|
Not recorded | UXU (4~{S})-1-[3,5-bis(fluoranyl)phenyl]-5,5-bis(fluoranyl)-3-methylsulfonyl-4,6-dihydrocyclopenta[c]thiophen-4-ol × 1 DMS DIMETHYL SULFOXIDE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；22% PEG 3350, 0.1 M BIS TRIS pH 6.0
|
Resolution 1.71 Å R-free 0.227 |
| 8CK4 STRUCTURE OF HIF2A-ARNT HETERODIMER IN COMPLEX WITH (4S)-1-(3,5-difluorophenyl)-5,5-difluoro-3-methanesulfonyl-4,5,6,7-tetrahydro-2-benzothiophen-4-ol Deposited 2023-02-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
|
Not recorded | UY3 (4~{S})-1-[3,5-bis(fluoranyl)phenyl]-5,5-bis(fluoranyl)-3-methylsulfonyl-6,7-dihydro-4~{H}-2-benzothiophen-4-ol × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；17% (w/v) PEG3350 ; 0.1 M BisTris pH 6.25
|
Resolution 2.29 Å R-free 0.251 |
| 8CK8 STRUCTURE OF HIF2A-ARNT HETERODIMER IN COMPLEX WITH (S)-1-Cyclohexyloxy-5,5-difluoro-3-methanesulfonyl-5,6-dihydro-4H-cyclopenta[c]thiophen-4-ol Deposited 2023-02-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
356–470（115 aa）
|
Not recorded | UYF (4~{S})-1-cyclohexyloxy-5,5-bis(fluoranyl)-3-methylsulfonyl-4,6-dihydrocyclopenta[c]thiophen-4-ol × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；17% (w/v) PEG3350 ; 0.1 M BisTris pH 6.25
|
Resolution 2.30 Å R-free 0.276 |
| 8G4A Human ARNT PAS-B complexed with KG-548 small molecule Deposited 2023-02-08 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
356–470（115 aa）
Chain B
356–470（115 aa）
|
Not recorded | YL8 5-[3,5-bis(trifluoromethyl)phenyl]-2H-tetrazole × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；298 K；0.1 M MES pH 6.5, 1.6 M magnesium sulfate, 2:1 (protein:condition)
|
Resolution 1.97 Å R-free 0.229 |
| 8XS6 Crystal structure of the DNA-bound AHR-ARNT heterodimer in complex with Tapinarof Deposited 2024-01-09 | Assembly 1 Protein–DNA Heteromer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
85–465（381 aa）
|
Not recorded | A1LWH Tapinarof × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289 K；potassium citrate tribasic monohydrate, PEG3350
|
Resolution 2.95 Å R-free 0.271 |
| 8XS7 Crystal structure of the DNA-bound AHR-ARNT heterodimer in complex with FICZ Deposited 2024-01-09 | Assembly 1 Protein–DNA Heteromer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
85–465（381 aa）
|
Not recorded | DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 A1LWI 5,11-dihydroindolo[3,2-b]carbazole-12-carbaldehyde × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289 K；potassium citrate tribasic monohydrate, PEG3350
|
Resolution 2.77 Å R-free 0.267 |
| 8XS8 Crystal structure of the DNA-bound AHR-ARNT heterodimer in complex with Benzo[a]pyrene Deposited 2024-01-09 | Assembly 1 Protein–DNA Heteromer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
85–465（381 aa）
|
Not recorded | W62 benzo[a]pyrene × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289 K；potassium citrate tribasic monohydrate, PEG3350
|
Resolution 3.11 Å R-free 0.230 |
| 8XS9 Crystal structure of the DNA-bound AHR-ARNT heterodimer in complex with beta-Naphthoflavone Deposited 2024-01-09 | Assembly 1 Protein–DNA Heteromer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
85–465（381 aa）
|
Not recorded | DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 A1LWJ (3R)-3-phenyl-2,3-dihydrobenzo[f]chromen-1-one × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289 K；potassium citrate tribasic monohydrate, PEG3350
|
Resolution 2.80 Å R-free 0.264 |
| 8XSA Crystal structure of the DNA-bound AHR-ARNT heterodimer in complex with Indigo Deposited 2024-01-09 | Assembly 1 Protein–DNA Heteromer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
85–465（381 aa）
|
Not recorded | DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 A1LWK 2-(3-oxidanyl-1H-indol-2-yl)indol-3-one × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289 K；potassium citrate tribasic monohydrate, PEG3350
|
Resolution 2.60 Å R-free 0.249 |
| 8XSB Crystal structure of the DNA-bound AHR-ARNT heterodimer in complex with Indirubin Deposited 2024-01-09 | Assembly 1 Protein–DNA Heteromer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
85–465（381 aa）
|
Not recorded | JY6 (3~{Z})-3-(3-oxidanylidene-1~{H}-indol-2-ylidene)-1~{H}-indol-2-one × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289 K；potassium citrate tribasic monohydrate, PEG3350
|
Resolution 3.06 Å R-free 0.240 |
| 9I64 Crystal structure of Casdatifan bound to HIF2a-B*:ARNT-B* complex Deposited 2025-01-29 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
355–470（116 aa）
|
Not recorded | A1I0H (5~{R},6~{S},8~{R})-3,5,6-tris(fluoranyl)-8-[(1~{S},2~{R})-2-fluoranyl-7-methylsulfonyl-1-oxidanyl-2,3-dihydro-1~{H}-inden-4-yl]-5,6,7,8-tetrahydronaphthalene-1-carbonitrile × 1 FMT FORMIC ACID × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.10 M Sodium Acetate, 1.25 M Sodium Fluoride
|
Resolution 1.56 Å R-free 0.214 |
| 9OF0 Cryo-EM Structure of Human HIF-2a-ARNT Complexed on 20-bp HRE Deposited 2025-04-29 | Assembly 1 Protein–DNA Heteromer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain B
91–470（380 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；50mM HEPES, 150mM NaCl, 5mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9OF2 Dimer of HIF-2a-ARNT Heterodimers Complexed on 51-bp HRE/HAS Deposited 2025-04-29 | Assembly 1 Protein–DNA Heteromer；Protein × 4 PDB declaration: hexameric（6） Consistent with all polymers |
Chain D
91–470（380 aa）
Chain F
91–470（380 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；50mM HEPES, 150mM NaCl, 5mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 9OFU Dimer of HIF-1a-ARNT Heterodimers Complexed on 52-bp HRE/HAS Deposited 2025-04-30 | Assembly 1 Protein–DNA Heteromer；Protein × 4 PDB declaration: hexameric（6） Consistent with all polymers |
Chain B
91–470（380 aa）
Chain D
91–470（380 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4；50mM HEPES, 150mM NaCl, 5mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |