Current Protein Identity:P27692
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2EXU Crystal Structure of Saccharomyces cerevisiae transcription elongation factors Spt4-Spt5NGN domain Deposited 2005-11-08 | Assembly 1 Insufficient information Homooligomer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
285–375(91 aa)
Fragment:Spt4, Spt5 NGN domain
|
Not recorded | ZN ZINC ION × 2 EOH ETHANOL × 4 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8.8;293 K;30% EtOH, 50mM NaCl, 100mM Tris-HCl, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.23 Å R-free 0.221 |
| 4YTK Structure of the KOW1-Linker1 domain of Transcription Elongation Factor Spt5 Deposited 2015-03-17 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
382–511(130 aa)
Fragment:UNP Residues 382-511
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 8;293 K;0.1 M Tris-HCl, pH 8.0, 30% (w/v) PEG 3350
|
Resolution 1.09 Å R-free 0.199 |
| 4YTL Structure of the KOW2-KOW3 Domain of Transcription Elongation Factor Spt5. Deposited 2015-03-17 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
534–632(99 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 5;293 K;0.1 M sodium citrate (pH 5.0), 0.5 M ammonium sulfate, and 1.0 M lithium sulfate
|
Resolution 1.60 Å R-free 0.203 |
| 4YTL Structure of the KOW2-KOW3 Domain of Transcription Elongation Factor Spt5. Deposited 2015-03-17 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
534–632(99 aa)
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 3 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 5;293 K;0.1 M sodium citrate (pH 5.0), 0.5 M ammonium sulfate, and 1.0 M lithium sulfate
|
Resolution 1.60 Å R-free 0.203 |
| 7NKX RNA polymerase II-Spt4/5-nucleosome-Chd1 structure Deposited 2021-02-19 | Assembly 1 Other combination Heteromer;Protein × 23 PDB declaration: 26-meric(26) Consistent with all polymers |
Chain Z
1–1063(1063 aa)
|
Not recorded | ZN ZINC ION × 8 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8JCH Cryo-EM structure of yeast Rat1-bound Pol II pre-termination transcription complex 1 (Pol II Rat1-PTTC1) Deposited 2023-05-11 | Assembly 1 Other combination Heteromer;Protein × 15 PDB declaration: 18-meric(18) Consistent with all polymers |
Chain W
1–1063(1063 aa)
|
Not recorded | ZN ZINC ION × 8 MG MAGNESIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 8K5P Cryo-EM structure of yeast Rat1-bound Pol II pre-termination transcription complex 2 (Pol II Rat1-PTTC2) Deposited 2023-07-23 | Assembly 1 Other combination Heteromer;Protein × 15 PDB declaration: 18-meric(18) Consistent with all polymers |
Chain W
1–1063(1063 aa)
|
Not recorded | ZN ZINC ION × 8 MG MAGNESIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8RAM Structure of Sen1 bound RNA Polymerase II pre-termination complex Deposited 2023-12-01 | Assembly 1 Other combination Heteromer;Protein × 16 PDB declaration: 19-meric(19) Consistent with all polymers |
Chain Z
1–1063(1063 aa)
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 8RAP Structure of Sen1-ADP.BeF3 bound RNA Polymerase II pre-termination complex Deposited 2023-12-01 | Assembly 1 Other combination Heteromer;Protein × 16 PDB declaration: 19-meric(19) Consistent with all polymers |
Chain Z
1–1063(1063 aa)
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å |