当前蛋白身份：P29372
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1BNK HUMAN 3-METHYLADENINE DNA GLYCOSYLASE COMPLEXED TO DNA 提交 1998-07-29 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
80–295（216 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.5；pH 7.5
|
分辨率 2.70 Å R-free 0.260 |
| 1EWN CRYSTAL STRUCTURE OF THE HUMAN AAG DNA REPAIR GLYCOSYLASE COMPLEXED WITH 1,N6-ETHENOADENINE-DNA 提交 2000-04-26 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
80–298（219 aa）
片段：E125Q
|
未记录 | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；295 K；PEG 4000, MgCl2, Tris-HCl, glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 2.10 Å R-free 0.259 |
| 1F4R CRYSTAL STRUCTURE OF THE HUMAN AAG DNA REPAIR GLYCOSYLASE COMPLEXED WITH 1,N6-ETHENOADENINE-DNA 提交 2000-06-08 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
80–298（219 aa）
|
未记录 | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；PEG 4000, magnesium chloride, Tris-HCl, glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.40 Å R-free 0.276 |
| 1F6O CRYSTAL STRUCTURE OF THE HUMAN AAG DNA REPAIR GLYCOSYLASE COMPLEXED WITH DNA 提交 2000-06-22 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
80–298（219 aa）
片段：C-TERMINAL FRAGMENT
|
未记录 | NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；PEG 8000, magnesium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.40 Å R-free 0.282 |
| 3QI5 Crystal structure of human alkyladenine DNA glycosylase in complex with 3,N4-ethenocystosine containing duplex DNA 提交 2011-01-26 | Assembly 1 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 A
84–298（215 aa）
片段：delta79AAG
|
未记录 | MN MANGANESE (II) ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；295 K；The 3,N4-ethenocytosine (EDC) containing DNA duplex was prepared by annealing the EDC containing 13-mer crystallization oligonucleotide ('5-GAC ATG (EDC)TT GCC T-3') with its complementary strand that contained G opposite EDC (5'-GGC AAG CAT GTC A-3'). The delta79AAG-EDC complexes were prepared by mixing equimolar ratios of delta79AAG and EDC:G 13-mer DNA duplex at the final protein-DNA complex concentration of 0.3 mM in the complex buffer (20 mM Hepes-NaOH pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 5% v/v glycerol and 1 mM DTT). The complex was incubated on ice for 15 min and used for crystallization. The crystals were obtained upon mixing 1 uL of complex and 1 ul of the reservoir solution (100 mM sodium cacodylate pH 6.0, 200 mM manganese chloride and 20% polyethylene glycol (PEG)-3350) over 0.5 ml of the reservoir solution, followed by incubation for 2 days, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 2.20 Å R-free 0.284 |
| 3QI5 Crystal structure of human alkyladenine DNA glycosylase in complex with 3,N4-ethenocystosine containing duplex DNA 提交 2011-01-26 | Assembly 2 蛋白–DNA 单体；蛋白 × 1 PDB 声明：trimeric（3） 与全部聚合物一致 |
链 B
84–298（215 aa）
片段：delta79AAG
|
未记录 | MN MANGANESE (II) ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；295 K；The 3,N4-ethenocytosine (EDC) containing DNA duplex was prepared by annealing the EDC containing 13-mer crystallization oligonucleotide ('5-GAC ATG (EDC)TT GCC T-3') with its complementary strand that contained G opposite EDC (5'-GGC AAG CAT GTC A-3'). The delta79AAG-EDC complexes were prepared by mixing equimolar ratios of delta79AAG and EDC:G 13-mer DNA duplex at the final protein-DNA complex concentration of 0.3 mM in the complex buffer (20 mM Hepes-NaOH pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 5% v/v glycerol and 1 mM DTT). The complex was incubated on ice for 15 min and used for crystallization. The crystals were obtained upon mixing 1 uL of complex and 1 ul of the reservoir solution (100 mM sodium cacodylate pH 6.0, 200 mM manganese chloride and 20% polyethylene glycol (PEG)-3350) over 0.5 ml of the reservoir solution, followed by incubation for 2 days, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 2.20 Å R-free 0.284 |
| 3UBY Crystal structure of human alklyadenine DNA glycosylase in a lower and higher-affinity complex with DNA 提交 2011-10-25 | Assembly 1 蛋白–DNA 同源多聚体；蛋白 × 2 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
84–298（215 aa）
片段：DELTA79AAG
链 B
84–298（215 aa）
片段：DELTA79AAG
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；298 K；An equimolar ratio of delta79AAG and 13-mer single-stranded (ss) EDC DNA were mixed to form a protein-DNA complex concentration of 0.3 mM in the complex buffer (20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 5% v/v glycerol and 1 mM DTT). The complex was incubated on ice for 15 min and used for crystallization. Crystals were obtained upon mixing 1 uL of protein-DNA complex and 1 uL of reservoir solution (100 mM BIS-TRIS, pH 5.5, 200 mM cesium chloride and 20% polyethylene glycol (PEG) 3350) over 0.5 ml of reservoir solution. Crystals appeared after incubation for 14 days at 22 degrees C, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.00 Å R-free 0.265 |
| 7XFH Structure of nucleosome-AAG complex (A-30I, post-catalytic state) 提交 2022-04-01 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 9 PDB 声明：undecameric（11） 与全部聚合物一致 |
链 K
1–298（298 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 NITROGEN
|
分辨率 2.90 Å |
| 7XFJ Structure of nucleosome-AAG complex (T-50I, post-catalytic state) 提交 2022-04-01 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 9 PDB 声明：undecameric（11） 与全部聚合物一致 |
链 K
1–298（298 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 NITROGEN
|
分辨率 3.00 Å |
| 7XFM Structure of nucleosome-AAG complex (A-53I, post-catalytic state) 提交 2022-04-01 | Assembly 1 蛋白–DNA 异源复合物；蛋白 × 9 PDB 声明：undecameric（11） 与全部聚合物一致 |
链 K
1–298（298 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 NITROGEN
|
分辨率 3.10 Å |