当前蛋白身份：P31016
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相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1BE9 THE THIRD PDZ DOMAIN FROM THE SYNAPTIC PROTEIN PSD-95 IN COMPLEX WITH A C-TERMINAL PEPTIDE DERIVED FROM CRIPT. 提交 1998-05-20 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
295–420（126 aa）
片段：THE THIRD PDZ DOMAIN OF PSD-95
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.5；0.8 M SODIUM CITRATE, 0.1 M HEPES, PH 7.5
|
分辨率 1.82 Å R-free 0.278 |
| 1BFE THE THIRD PDZ DOMAIN FROM THE SYNAPTIC PROTEIN PSD-95 提交 1998-05-20 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：THE THIRD PDZ DOMAIN OF PSD-95
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.5；0.8 M SODIUM CITRATE, 0.1 M HEPES, PH 7.5
|
分辨率 2.30 Å R-free 0.315 |
| 1IU0 The first PDZ domain of PSD-95 提交 2002-02-18 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
61–151（91 aa）
片段：PDZ1 domain
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 6.8；303 K；离子强度（mmCIF原始值）100mM KCl；压力 1
NMR样品组成
20mM KPi | 10% D2O, 90% H2O
NMR样品组成
20mM KPi | 100% D2O
|
分辨率未提供 |
| 1IU2 The first PDZ domain of PSD-95 提交 2002-02-19 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
61–151（91 aa）
片段：PDZ1 domain
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 6.8；303 K；离子强度（mmCIF原始值）100mM KCl；压力 1
NMR样品组成
20mM KPi, 100mM KCl | 10% D2O, 90% H2O
NMR样品组成
20mM KPi, 100mM KCl | 100% D2O
|
分辨率未提供 |
| 1JXM CRYSTAL STRUCTURE OF THE GMP BOUND SH3-HOOK-GK FRAGMENT OF PSD-95 提交 2001-09-07 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
430–724（295 aa）
片段：SH3-HOOK-GK
|
未记录 | 5GP GUANOSINE-5'-MONOPHOSPHATE × 1 GAI GUANIDINE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.7；277 K；2-methyl-2,4-pentanediol, HEPES buffer, guanidine, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K
|
分辨率 2.00 Å R-free 0.260 |
| 1JXO Crystal Structure of the SH3-HOOK-GK Fragment of PSD-95 提交 2001-09-07 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
430–724（295 aa）
片段：SH3-HOOK-GK
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；2-methyl-2,4-pentanediol, MES buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 2.30 Å R-free 0.264 |
| 1JXO Crystal Structure of the SH3-HOOK-GK Fragment of PSD-95 提交 2001-09-07 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
430–724（295 aa）
片段：SH3-HOOK-GK
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.3；277 K；2-methyl-2,4-pentanediol, MES buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 2.30 Å R-free 0.264 |
| 1KJW SH3-Guanylate Kinase Module from PSD-95 提交 2001-12-05 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
430–724（295 aa）
片段：SH3 Domain/Guanylate Kinase Domain
|
未记录 | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；288 K；Ammonium sulfate, PEG 4000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 15K
|
分辨率 1.80 Å R-free 0.250 |
| 1QLC Solution structure of the second PDZ domain of Postsynaptic Density-95 提交 1999-08-25 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
155–249（95 aa）
片段：THE SECOND PDZ DOMAIN
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 6；303 K；离子强度（mmCIF原始值）100；压力 1
|
分辨率未提供 |
| 1RGR Cyclic Peptides Targeting PDZ Domains of PSD-95: Structural Basis for Enhanced Affinity and Enzymatic Stability 提交 2003-11-12 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
62–154（93 aa）
片段：PDZ1 domain of PSD-95
|
未记录 | BAL BETA-ALANINE × 1 | SOLUTION NMR |
NMR样品组成
1mM PZ1 U-15N,13C, 3mM peptide, 10 mM phospate buffer 150 mM NaCl, pH 6.8 | 90% H2O/10% D2O
|
分辨率未提供 |
| 1TP3 PDZ3 domain of PSD-95 protein complexed with KKETPV peptide ligand 提交 2004-06-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ 3; residues 302-402
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.9；295 K；1.0 M sodium citrate, 0.1 M HEPES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 1.99 Å R-free 0.296 |
| 1TP5 Crystal structure of PDZ3 domain of PSD-95 protein complexed with a peptide ligand KKETWV 提交 2004-06-15 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：third PDZ domain (residues 302-402)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.9；288 K；1.0 M sodium citrate, 0.1 M HEPES, 10 mM sodium chloride, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
分辨率 1.54 Å R-free 0.229 |
| 1TP5 Crystal structure of PDZ3 domain of PSD-95 protein complexed with a peptide ligand KKETWV 提交 2004-06-15 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：third PDZ domain (residues 302-402)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.9；288 K；1.0 M sodium citrate, 0.1 M HEPES, 10 mM sodium chloride, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 288K
|
分辨率 1.54 Å R-free 0.229 |
| 1TQ3 Higher resolution crystal structure of the third PDZ domain of post synaptic PSD-95 protein 提交 2004-06-16 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：third PDZ domain (residues 302-402)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.9；295 K；1.0 M sodium citrate, 0.1 M HEPES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 1.89 Å R-free 0.296 |
| 2KA9 Solution structure of PSD-95 PDZ12 complexed with cypin peptide 提交 2008-11-03 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
61–249（189 aa）
片段：N-terminal PDZ12 domain
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 6.5；303 K；离子强度（mmCIF原始值）100；压力 ambient
NMR样品组成
1mM [U-15N] PSD95 PDZ12, 1mM cypin peptide, 90% H2O, 10% D2O | 90% H2O/10% D2O
|
分辨率未提供 |
| 2MHO Solution State Structure PSD-95 PDZ1 with 5HT2C Receptor peptide 提交 2013-11-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
60–155（96 aa）
片段：PDZ 1, UNP residues 60-155
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 6.3；298 K；离子强度（mmCIF原始值）20；压力 ambient
NMR样品组成
0.5 mM [U-13C; U-15N] protein_1, 2.5 mM protein_2, 1 mM DTT, 0.01 w/v sodium azide, 20 mM sodium phosphate, 90% H2O/10% D2O
|
分辨率未提供 |
| 2XKX Single particle analysis of PSD-95 in negative stain 提交 2010-07-15 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–724（724 aa）
链 B
1–724（724 aa）
|
突变：YES 突变：YES | 未记录非水小分子 | 未声明 |
cryo-EM缓冲液
20MM TRIS/HCL, 5MM DTT, 1MM EDTA,；pH 7.5；20MM TRIS/HCL, 5MM DTT, 1MM EDTA,
|
分辨率 22.90 Å |
| 3GSL Crystal structure of PSD-95 tandem PDZ domains 1 and 2 提交 2009-03-27 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
61–249（189 aa）
片段：PDZ domains 1 and 2: UNP residues 61-249
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；35% v/v 2-methyl-2,4-pentanediol, 0.1M Tris-HCl pH 7.0. 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 2.05 Å R-free 0.281 |
| 3GSL Crystal structure of PSD-95 tandem PDZ domains 1 and 2 提交 2009-03-27 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
61–249（189 aa）
片段：PDZ domains 1 and 2: UNP residues 61-249
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；35% v/v 2-methyl-2,4-pentanediol, 0.1M Tris-HCl pH 7.0. 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
分辨率 2.05 Å R-free 0.281 |
| 3WP0 Crystal structure of Dlg GK in complex with a phosphor-Lgl2 peptide 提交 2014-01-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
531–713（183 aa）
片段：UNP residues 533-713
|
未记录 | GOL GLYCEROL × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.8；291 K；0.2M lithium chloride, 20% PEG3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
分辨率 2.04 Å R-free 0.202 |
| 3WP1 Phosphorylation-dependent interaction between tumor suppressors Dlg and Lgl 提交 2014-01-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
531–713（183 aa）
片段：UNP residues 531-713
|
未记录 | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.80 Å R-free 0.268 |
| 5D13 Third PDZ domain (PDZ3) of PSD-95 complexed with CFMOC-KKETEV peptide 提交 2015-08-03 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：unp residues 302-402
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；295 K；20 mM HEPES
100 mM NaCl
1.8 M tri-sodium citrate
|
分辨率 2.15 Å R-free 0.257 |
| 5D13 Third PDZ domain (PDZ3) of PSD-95 complexed with CFMOC-KKETEV peptide 提交 2015-08-03 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
302–402（101 aa）
片段：unp residues 302-402
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；295 K；20 mM HEPES
100 mM NaCl
1.8 M tri-sodium citrate
|
分辨率 2.15 Å R-free 0.257 |
| 5D13 Third PDZ domain (PDZ3) of PSD-95 complexed with CFMOC-KKETEV peptide 提交 2015-08-03 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 C
302–402（101 aa）
片段：unp residues 302-402
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；295 K；20 mM HEPES
100 mM NaCl
1.8 M tri-sodium citrate
|
分辨率 2.15 Å R-free 0.257 |
| 5D13 Third PDZ domain (PDZ3) of PSD-95 complexed with CFMOC-KKETEV peptide 提交 2015-08-03 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
302–402（101 aa）
片段：unp residues 302-402
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.2；295 K；20 mM HEPES
100 mM NaCl
1.8 M tri-sodium citrate
|
分辨率 2.15 Å R-free 0.257 |
| 5GNV Structure of PSD-95/MAP1A complex reveals unique target recognition mode of MAGUK GK domain 提交 2016-07-25 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
531–713（183 aa）
片段：UNP residues 531-713
|
未记录 | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
EVAPORATION；pH 6.5；289 K；0.2M lithium sulfate, 0.1M Bis-Tris pH 6.5 and 25%(w/v) polyethylene glycol 3350
|
分辨率 2.60 Å R-free 0.257 |
| 5HDY The third PDZ domain from the synaptic protein PSD-95 提交 2016-01-05 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
未记录 | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.9；289 K；Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against microliters of crystallization buffer (1.0 M sodium citrate) at 289 K. Diamond-shaped crystals appeared either spontaneously or with microseeding after 1-5 days and grew to 100-200 microns in length over several weeks.
|
分辨率 1.80 Å R-free 0.220 |
| 5HEB The third PDZ domain from the synaptic protein PSD-95 in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
未记录 | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.65 Å R-free 0.195 |
| 5HED The third PDZ domain from the synaptic protein PSD-95 in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-05 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：T-2F | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.1；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.125 M sodium citrate, pH 7.1. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.70 Å R-free 0.197 |
| 5HET The third PDZ domain from the synaptic protein PSD-95 (G330T mutant) 提交 2016-01-06 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：G330T | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Reservoir solution contained 1.05 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (7 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 2.00 Å R-free 0.213 |
| 5HEY The third PDZ domain from the synaptic protein PSD-95 (G330T mutant) in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：G330T | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (8 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.50 Å R-free 0.203 |
| 5HF1 The third PDZ domain from the synaptic protein PSD-95 (G330T mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：G330T | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.8；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 6.8. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.75 Å R-free 0.218 |
| 5HF4 The third PDZ domain from the synaptic protein PSD-95 (H372A mutant) 提交 2016-01-06 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：H372A | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Reservoir solution contained 0.95 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.75 Å R-free 0.221 |
| 5HFB The third PDZ domain from the synaptic protein PSD-95 (H372A mutant) in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：H372A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (8 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.62 Å R-free 0.203 |
| 5HFC The third PDZ domain from the synaptic protein PSD-95 (H372A mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：H372A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.05 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (7 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.85 Å R-free 0.207 |
| 5HFD The third PDZ domain from the synaptic protein PSD-95 (G330T, H372A double mutant) 提交 2016-01-06 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：G330T H372A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Reservoir solution contained 1 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (13 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.60 Å R-free 0.197 |
| 5HFE The third PDZ domain from the synaptic protein PSD-95 (G330T, H372A double mutant) in complex with a C-terminal peptide derived from CRIPT 提交 2016-01-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：G330T H372A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.25 M sodium citrate, pH 7.0. Equal amounts (1.5 microliters) of protein (9 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.80 Å R-free 0.211 |
| 5HFF The third PDZ domain from the synaptic protein PSD-95 (G330T, H372A double mutant) in complex with a mutant C-terminal peptide derived from CRIPT (T-2F) 提交 2016-01-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
302–402（101 aa）
片段：PDZ-3 domain (UNP residues 302-402)
|
突变：G330T, H372A | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 6.75；289 K；Peptide was included in protein buffer to a final molar ratio of 2:1 relative to protein. Reservoir solution contained 1.2 M sodium citrate, pH 6.75. Equal amounts (1.5 microliters) of protein (7 mg/mL) and reservoir solution were mixed and equillibrated against 500 microliters of crystallization buffer.
|
分辨率 1.75 Å R-free 0.221 |
| 5MZ7 Crystal Structure of the third PDZ domain from the synaptic protein PSD-95 with incorporated Azidohomoalanine 提交 2017-01-31 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
303–402（100 aa）
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1.1 M sodium citrate, 0.1 M HEPES
|
分辨率 1.53 Å R-free 0.221 |
| 5MZ7 Crystal Structure of the third PDZ domain from the synaptic protein PSD-95 with incorporated Azidohomoalanine 提交 2017-01-31 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
303–402（100 aa）
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1.1 M sodium citrate, 0.1 M HEPES
|
分辨率 1.53 Å R-free 0.221 |
| 5MZ7 Crystal Structure of the third PDZ domain from the synaptic protein PSD-95 with incorporated Azidohomoalanine 提交 2017-01-31 | Assembly 3 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 C
303–402（100 aa）
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1.1 M sodium citrate, 0.1 M HEPES
|
分辨率 1.53 Å R-free 0.221 |
| 5MZ7 Crystal Structure of the third PDZ domain from the synaptic protein PSD-95 with incorporated Azidohomoalanine 提交 2017-01-31 | Assembly 4 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 D
303–402（100 aa）
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；1.1 M sodium citrate, 0.1 M HEPES
|
分辨率 1.53 Å R-free 0.221 |
| 5W72 Impact of IR active probes on PDZ3 and its ligand binding studied by NMR and X-ray crystallography 提交 2017-06-19 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
301–402（102 aa）
|
非标准单体：是（mmCIF未提供具体位点） | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 6.8；298 K；离子强度（mmCIF原始值）0；压力 1
NMR样品组成
0.5 mM [U-98% 13C; U-98% 15N] PDZ3, 50 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
|
分辨率未提供 |
| 5YPO Crystal structure of PSD-95 GK domain in complex with phospho-SAPAP peptide 提交 2017-11-02 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
531–713（183 aa）
|
未记录 | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 5.5；289 K；Condition: 0.1 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 17% w/v Polyethylene glycol 10000
|
分辨率 2.29 Å R-free 0.226 |
| 5YPO Crystal structure of PSD-95 GK domain in complex with phospho-SAPAP peptide 提交 2017-11-02 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
531–713（183 aa）
|
未记录 | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 5.5；289 K；Condition: 0.1 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 17% w/v Polyethylene glycol 10000
|
分辨率 2.29 Å R-free 0.226 |
| 5YPR Crystal Structure of PSD-95 SH3-GK domain in complex with a synthesized inhibitor 提交 2017-11-02 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
426–721（296 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 8；277 K；0.1M tris pH 8.0, 35% tert-Butanol
|
分辨率 2.35 Å R-free 0.264 |
| 7CQF Crystal structure of PSD-95 PDZ3 fused with ADAM22 C-terminal peptide 提交 2020-08-10 | Assembly 1 信息不足 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
309–422（114 aa）
|
未记录 | CL CHLORIDE ION × 1 CHT CHOLINE ION × 1 EDO 1,2-ETHANEDIOL × 4 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.2 M choline chloride, 0.1 M Tris-HCl (pH 7.5), 14 % (w/v) PEG 2000 MME
|
分辨率 1.80 Å R-free 0.205 |
| 7F7G a linear Peptide Inhibitors in complex with GK domain 提交 2021-06-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
531–713（183 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；289 K；(0.02 M Citric acid, 0.08 M BIS-TRIS propane / pH 8.8), 16% w/v Polyethylene glycol 3,350
|
分辨率 2.45 Å R-free 0.256 |
| 7F7G a linear Peptide Inhibitors in complex with GK domain 提交 2021-06-29 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
531–713（183 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；289 K；(0.02 M Citric acid, 0.08 M BIS-TRIS propane / pH 8.8), 16% w/v Polyethylene glycol 3,350
|
分辨率 2.45 Å R-free 0.256 |
| 7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
531–713（183 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)
|
分辨率 2.60 Å R-free 0.262 |
| 7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
531–713（183 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)
|
分辨率 2.60 Å R-free 0.262 |
| 7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
531–713（183 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)
|
分辨率 2.60 Å R-free 0.262 |
| 7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29 | Assembly 4 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 D
531–713（183 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)
|
分辨率 2.60 Å R-free 0.262 |
| 7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29 | Assembly 5 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
531–713（183 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)
|
分辨率 2.60 Å R-free 0.262 |
| 7F7I Stapled Peptide Inhibitor in complex with PSD95 GK domain 提交 2021-06-29 | Assembly 6 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 F
531–713（183 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；289 K；35 % w/v PEP 629, 100 mM HEPES (pH7.5)
|
分辨率 2.60 Å R-free 0.262 |
| 9IUI Crystal structure of PSD-95 GK domain in complex with GK_FingR 提交 2024-07-21 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
531–713（183 aa）
片段：GK domain
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 5；289 K；0.1M Sodium Citrate pH 5.0, 20% PEG 20000
|
分辨率 1.93 Å R-free 0.278 |
| 9IUI Crystal structure of PSD-95 GK domain in complex with GK_FingR 提交 2024-07-21 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 C
531–713（183 aa）
片段：GK domain
|
未记录 | GOL GLYCEROL × 2 PG4 TETRAETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 5；289 K；0.1M Sodium Citrate pH 5.0, 20% PEG 20000
|
分辨率 1.93 Å R-free 0.278 |
| 9UDJ Crystal structure of PSD95 in complex with 4-dimethylaminophenol 提交 2025-04-07 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
533–713（181 aa）
片段：Guanylate kinase-like
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；289.15 K；0.1 M Sodium citrate tribasic dihydrate (pH 5.5), 18% w/v Polyethylene glycol 3,350.
|
分辨率 2.44 Å R-free 0.274 |
| 9UDJ Crystal structure of PSD95 in complex with 4-dimethylaminophenol 提交 2025-04-07 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
533–713（181 aa）
片段：Guanylate kinase-like
|
未记录 | A1EOW 4-(dimethylamino)phenol × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；289.15 K；0.1 M Sodium citrate tribasic dihydrate (pH 5.5), 18% w/v Polyethylene glycol 3,350.
|
分辨率 2.44 Å R-free 0.274 |