当前蛋白身份：P31324
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 3TNP Structure and Allostery of the PKA RIIb Tetrameric Holoenzyme 提交 2011-09-01 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 B
1–416（416 aa）
链 E
1–416（416 aa）
|
突变：R230K 突变：R230K | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；The RII (R230K)2:C2 holoenzyme complex was crystallized at room temperature in 10% PEG8000, 8% ethylene glycol, pH7.5 HEPES buffer by using vapor diffusion at a 1:1 ratio of protein to crystallization solution. VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.30 Å R-free 0.235 |
| 3TNP Structure and Allostery of the PKA RIIb Tetrameric Holoenzyme 提交 2011-09-01 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
1–416（416 aa）
|
突变：R230K | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；The RII (R230K)2:C2 holoenzyme complex was crystallized at room temperature in 10% PEG8000, 8% ethylene glycol, pH7.5 HEPES buffer by using vapor diffusion at a 1:1 ratio of protein to crystallization solution. VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.30 Å R-free 0.235 |
| 3TNP Structure and Allostery of the PKA RIIb Tetrameric Holoenzyme 提交 2011-09-01 | Assembly 3 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 E
1–416（416 aa）
|
突变：R230K | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；The RII (R230K)2:C2 holoenzyme complex was crystallized at room temperature in 10% PEG8000, 8% ethylene glycol, pH7.5 HEPES buffer by using vapor diffusion at a 1:1 ratio of protein to crystallization solution. VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 2.30 Å R-free 0.235 |
| 3TNQ Structure and Allostery of the PKA RIIb Tetrameric Holoenzyme 提交 2011-09-01 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–416（416 aa）
|
突变：R230K 非标准单体：是（mmCIF未提供具体位点） | MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；The RII (R230K)2:C2 holoenzyme complex was crystallized at room temperature in 10% PEG8000, 8% ethylene glycol, pH7.5 HEPES buffer by using vapor diffusion at a 1:1 ratio of protein to crystallization solution. VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
分辨率 3.10 Å R-free 0.275 |
| 4WBB Single Turnover Autophosphorylation Cycle of the PKA RIIb Holoenzyme 提交 2014-09-02 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–416（416 aa）
|
非标准单体：是（mmCIF未提供具体位点） | CA CALCIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
BATCH MODE；298 K；The RIIb (R230K):C apo complex was crystallized in 20%PEG8000, 8% Ethylene Glycol, 20mM MESpH7.5 HEPES . To make CaATP binding holoenzyme crystals, the apo holoenzyme crystals were soaked with 20%PEG8000, 8% Ethylene Glycol, 20mM MESpH7.5 HEPES, 5mM CaCl2 and 1mM ATP at pH5.8 solution overnight
|
分辨率 2.80 Å R-free 0.279 |
| 4X6Q An Isoform-specific Myristylation Switch Targets RIIb PKA Holoenzymes to Membranes 提交 2014-12-08 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
1–416（416 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
BATCH MODE；298 K；The RIIb(R230K)2: myrC(K7C)2 holoenzyme
was concentrated to 12 mg/mL. The crystal used for structure determination was obtained from a 3 uL drop containing 1:1 protein to well solution with the well solution containing 10% PEG8000, 8% ethylene glycol, 20mM MES, at pH5.8.
|
分辨率 2.52 Å R-free 0.261 |