当前蛋白身份:P32081
重新检索
差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1CSP CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS MAJOR COLD SHOCK PROTEIN, CSPB: A UNIVERSAL NUCLEIC-ACID BINDING DOMAIN 提交 1993-05-12 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1–67(67 aa)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.45 Å |
| 1CSQ CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS MAJOR COLD SHOCK PROTEIN, CSPB: A UNIVERSAL NUCLEIC-ACID BINDING DOMAIN 提交 1993-05-12 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1–67(67 aa)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.70 Å |
| 1NMF MAJOR COLD-SHOCK PROTEIN, NMR, 20 STRUCTURES 提交 1996-02-05 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1–67(67 aa)
|
未记录 | 未记录非水小分子 | SOLUTION NMR | mmCIF 未提供已读取条件 | 分辨率未提供 |
| 1NMG MAJOR COLD-SHOCK PROTEIN, NMR, MINIMIZED AVERAGE STRUCTURE 提交 1996-02-05 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1–67(67 aa)
|
未记录 | 未记录非水小分子 | SOLUTION NMR | mmCIF 未提供已读取条件 | 分辨率未提供 |
| 2ES2 Crystal Structure Analysis of the Bacillus Subtilis Cold Shock Protein Bs-CspB in Complex with Hexathymidine 提交 2005-10-25 | Assembly 1 蛋白–DNA 单体;蛋白 × 1 PDB 声明:dimeric(2) 与全部聚合物一致 |
链 A
1–67(67 aa)
|
未记录 | CA CALCIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP;pH 6.5;293 K;0.2M calcium acetate
18% (w/v) PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
分辨率 1.78 Å R-free 0.223 |
| 2F52 Solution structure of cold shock protein CspB from Bacillus subtilis in complex with heptathymidine 提交 2005-11-25 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1–67(67 aa)
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 7;288 K;离子强度(mmCIF原始值)53mM buffer salt;压力 ambient
NMR样品组成
0.8mM CspB U-15N, 50mM Na-cacodylate, 3mM MgCl2, 1.2mM heptathymidine, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR样品组成
0.8mM CspB U-15N, 13C, 50mM Na-cacodylate, 3mM MgCl2, 1.2mM heptathymidine, 90% H2O, 10% D2O | 90% H2O/10% D2O
|
分辨率未提供 |
| 2I5L Crystal structure of Bacillus subtilis Cold Shock Protein variant Bs-CspB M1R/E3K/K65I 提交 2006-08-25 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 X
1–67(67 aa)
|
突变:M1R, E3K, K65I | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 8.5;293.15 K;protein solution: 20 mM TRIS pH 7.5, 50 mM sodium chloride, 3 mM magnesium chloride, 20.4 mg/ml protein.
crystallization buffer: 25 % PEG 3350, 0.2 M sodium carbonate, 0.1 M TRIS HCl pH 8.5. crystallization setup: 0.8 microliter protein solution:0.8 microliter reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
分辨率 2.55 Å R-free 0.244 |
| 2I5M Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R 提交 2006-08-25 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 X
1–67(67 aa)
|
突变:A46K, S48R | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.5;293.15 K;reservoir solution: 1.5 M lithium sulfate, 0.1 M TRIS pH 7.5, 15% glycerol for cryoprotection. protein solution: 20 mM TRIS pH 7.5, 50 mM NaCl, 3 mM MgCl2, 17.4 mg/ml protein. crystallization setup: 0.8 microliter protein solution:0.8 microliter reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
分辨率 2.30 Å R-free 0.227 |
| 2I5M Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R 提交 2006-08-25 | Assembly 2 蛋白同源多聚体 同源多聚体;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 X
1–67(67 aa)
|
突变:A46K, S48R | MG MAGNESIUM ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.5;293.15 K;reservoir solution: 1.5 M lithium sulfate, 0.1 M TRIS pH 7.5, 15% glycerol for cryoprotection. protein solution: 20 mM TRIS pH 7.5, 50 mM NaCl, 3 mM MgCl2, 17.4 mg/ml protein. crystallization setup: 0.8 microliter protein solution:0.8 microliter reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
分辨率 2.30 Å R-free 0.227 |
| 3PF4 Crystal structure of Bs-CspB in complex with r(GUCUUUA) 提交 2010-10-27 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1–67(67 aa)
|
未记录 | NA SODIUM ION × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 8.5;293.15 K;protein buffer: 50mM TRIS, 20mM Na-HEPES, pH 7.5; Bs-CspB.rGUCUUUA complex concentration: 50mg/ml; crystallization buffer: 30% (w/v) PEG 4000, 0.2M MgCl2, 0.1M TRIS pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
分辨率 1.38 Å R-free 0.194 |
| 3PF4 Crystal structure of Bs-CspB in complex with r(GUCUUUA) 提交 2010-10-27 | Assembly 2 蛋白–RNA 单体;蛋白 × 1 PDB 声明:dimeric(2) 与全部聚合物一致 |
链 B
1–67(67 aa)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 8.5;293.15 K;protein buffer: 50mM TRIS, 20mM Na-HEPES, pH 7.5; Bs-CspB.rGUCUUUA complex concentration: 50mg/ml; crystallization buffer: 30% (w/v) PEG 4000, 0.2M MgCl2, 0.1M TRIS pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
分辨率 1.38 Å R-free 0.194 |
| 3PF5 Crystal structure of Bs-CspB in complex with rU6 提交 2010-10-27 | Assembly 1 蛋白–RNA 单体;蛋白 × 1 PDB 声明:dimeric(2) 与全部聚合物一致 |
链 A
1–67(67 aa)
|
未记录 | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 8.5;293.15 K;protein buffer: 50mM TRIS, 20mM Na-HEPES, pH 7.5; Bs-CspB.rU6 complex concentration: 70mg/ml; crystallization buffer: 31% PEG 3350, 0.25M MgCl2, 0.1M TRIS pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
分辨率 1.68 Å R-free 0.235 |
| 3PF5 Crystal structure of Bs-CspB in complex with rU6 提交 2010-10-27 | Assembly 2 蛋白–RNA 单体;蛋白 × 1 PDB 声明:dimeric(2) 与全部聚合物一致 |
链 B
1–67(67 aa)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 8.5;293.15 K;protein buffer: 50mM TRIS, 20mM Na-HEPES, pH 7.5; Bs-CspB.rU6 complex concentration: 70mg/ml; crystallization buffer: 31% PEG 3350, 0.25M MgCl2, 0.1M TRIS pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
分辨率 1.68 Å R-free 0.235 |