当前蛋白身份：P32390
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 3DWL Crystal Structure of Fission Yeast Arp2/3 Complex Lacking the Arp2 Subunit 提交 2008-07-22 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
1–427（427 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；750 mM ammonium sulfate
50 mM sodium citrate
7 % glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 3.78 Å R-free 0.344 |
| 3DWL Crystal Structure of Fission Yeast Arp2/3 Complex Lacking the Arp2 Subunit 提交 2008-07-22 | Assembly 2 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 B
1–427（427 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 5.5；277 K；750 mM ammonium sulfate
50 mM sodium citrate
7 % glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
分辨率 3.78 Å R-free 0.344 |
| 6W17 Structure of Dip1-activated Arp2/3 complex with nucleated actin filament 提交 2020-03-03 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 17 PDB 声明：heptadecameric（17） 与蛋白数一致 |
链 A
1–427（427 aa）
|
未记录 | MG MAGNESIUM ION × 6 ATP ADENOSINE-5'-TRIPHOSPHATE × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 4 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.90 Å |
| 6W18 Structure of S. pombe Arp2/3 complex in inactive state 提交 2020-03-03 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 7 PDB 声明：heptameric（7） 与蛋白数一致 |
链 A
1–427（427 aa）
|
未记录 | ATP ADENOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE；4 uL of sample was applied to freshly glow-discharged grid. Excess sample was manually blotted off with a dry Whatman No.1 filter paper for 5-7 seconds. Immediately after the blotting step the sample containing grid was rapidly vitrified by plunge freezing into liquid ethane.
|
分辨率 4.20 Å |
| 8E9B Cryo-EM structure of S. pombe Arp2/3 complex in the branch junction 提交 2022-08-26 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 15 PDB 声明：pentadecameric（15） 与蛋白数一致 |
链 A
1–427（427 aa）
|
未记录 | MG MAGNESIUM ION × 10 ADP ADENOSINE-5'-DIPHOSPHATE × 9 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 8UXW Arp2/3 branch junction complex, ADP state 提交 2023-11-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 15 PDB 声明：pentadecameric（15） 与蛋白数一致 |
链 A
1–427（427 aa）
|
未记录 | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 10 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
分辨率 2.70 Å |
| 8UXX Arp2/3 branch junction complex, BeFx state 提交 2023-11-11 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 15 PDB 声明：pentadecameric（15） 与蛋白数一致 |
链 A
1–427（427 aa）
|
未记录 | ADP ADENOSINE-5'-DIPHOSPHATE × 9 MG MAGNESIUM ION × 10 BEF BERYLLIUM TRIFLUORIDE ION × 7 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE；The samples were incubated on the grid for 50 s and the extra solution was blotted using two Vitrobot filter papers (0.55/20 mm, Grade 595, Ted Pella) for 4 s at 0 blot force. The grids were plunged into liquid ethane at ~180 degrees C with a wait time of 0.5 s.
|
分辨率 3.20 Å |