Current Protein Identity:P32558
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3BIP Crystal structure of yeast Spt16 N-terminal Domain Deposited 2007-11-30 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–465(465 aa)
Fragment:residues 1-465
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION;pH 4.5;298 K;25% Pentaerythritol Ethoxylate (15/4 EO/OH), 100mM Sodium Acetate, pH 4.5, VAPOR DIFFUSION, temperature 298K
|
Resolution 1.94 Å R-free 0.244 |
| 3BIP Crystal structure of yeast Spt16 N-terminal Domain Deposited 2007-11-30 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
1–465(465 aa)
Fragment:residues 1-465
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION;pH 4.5;298 K;25% Pentaerythritol Ethoxylate (15/4 EO/OH), 100mM Sodium Acetate, pH 4.5, VAPOR DIFFUSION, temperature 298K
|
Resolution 1.94 Å R-free 0.244 |
| 3BIQ Crystal structure of yeast Spt16 N-terminal Domain Deposited 2007-11-30 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–465(465 aa)
Fragment:residues 1-465
|
Not recorded | GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION;pH 4.5;298 K;25% Pentaerythritol Ethoxylate (15/4 EO/OH), 100mM Sodium Acetate, pH 4.5, vapor diffusion, temperature 298K
|
Resolution 1.73 Å R-free 0.213 |
| 3BIT Crystal structure of yeast Spt16 N-terminal Domain Deposited 2007-11-30 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–451(451 aa)
Fragment:residues 1-451
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | CL CHLORIDE ION × 2 BME BETA-MERCAPTOETHANOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION;pH 4.5;278 K;35% PEG 300, 200mM NaCl, 100mM Sodium Acetate, pH 4.5, vapor diffusion, temperature 278K
|
Resolution 1.90 Å R-free 0.224 |
| 3BIT Crystal structure of yeast Spt16 N-terminal Domain Deposited 2007-11-30 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
1–451(451 aa)
Fragment:residues 1-451
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | CL CHLORIDE ION × 2 BME BETA-MERCAPTOETHANOL × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION;pH 4.5;278 K;35% PEG 300, 200mM NaCl, 100mM Sodium Acetate, pH 4.5, vapor diffusion, temperature 278K
|
Resolution 1.90 Å R-free 0.224 |
| 4IOY Structure of the Spt16 Middle Domain Reveals Functional Features of the Histone Chaperone FACT Deposited 2013-01-08 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain X
675–958(284 aa)
Fragment:Middle Domain (UNP residues 675-958)
|
Not recorded | PO4 PHOSPHATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6;277.15 K;0.1 M sodium/potassium phosphate, pH 6.0, 0.2 M sodium chloride, 40% PEG400, 10 mM TCEP-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K
|
Resolution 1.95 Å R-free 0.197 |
| 7NKY RNA Polymerase II-Spt4/5-nucleosome-FACT structure Deposited 2021-02-19 | Assembly 1 Other combination Heteromer;Protein × 24 PDB declaration: 27-meric(27) Consistent with all polymers |
Chain Q
1–1035(1035 aa)
|
Not recorded | ZN ZINC ION × 9 MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8XGC Structure of yeast replisome associated with FACT and histone hexamer, Composite map Deposited 2023-12-15 | Assembly 1 Protein–DNA Heteromer;Protein × 27 PDB declaration: 29-meric(29) Consistent with all polymers |
Chain L
1–1035(1035 aa)
|
Not recorded | ZN ZINC ION × 7 ADP ADENOSINE-5'-DIPHOSPHATE × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |