Current Protein Identity：P35408
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5YHL Crystal structure of the human prostaglandin E receptor EP4 in complex with Fab and an antagonist Br-derivative Deposited 2017-09-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
4–366（363 aa）
Fragment：UNP RESIDUES 4-217, 260-366
|
Mutation：N7Q, A62L, G106R, N177Q, 218-259 deletion | 8VL 4-[2-[[(2R)-2-(4-bromanylnaphthalen-1-yl)propanoyl]amino]-4-cyano-phenyl]butanoic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；24-31% PEG 300, 150 mM potassium sulfate, 100 mM MES (pH 5.5-6.5), 1% 1,2,3-heptanetriol, 0.2 mM ONO-AE3-208-Br, 2% DMSO
|
Resolution 4.20 Å R-free 0.359 |
| 5YWY Crystal structure of the human prostaglandin E receptor EP4 in complex with Fab and ONO-AE3-208 Deposited 2017-11-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
4–217（214 aa）
Chain A
260–366（107 aa）
|
Mutation：N7Q, A62L, G106R, N177Q, 218-259 deletion,N7Q, A62L, G106R, N177Q, 218-259 deletion Mutation：N7Q, A62L, G106R, N177Q, 218-259 deletion,N7Q, A62L, G106R, N177Q, 218-259 deletion | 7UR 4-[4-cyano-2-[[(2R)-2-(4-fluoranylnaphthalen-1-yl)propanoyl]amino]phenyl]butanoic acid × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
LIPIDIC CUBIC PHASE；293 K；24-31% PEG 300, 150 mM potassium sulfate, 100 mM MES pH 5.5-6.5, 1% 1,2,3-heptantoriol
|
Resolution 3.20 Å R-free 0.252 |
| 7D7M Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein Deposited 2020-10-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain A
4–217（214 aa）
Chain A
260–366（107 aa）
|
Mutation：N7Q, N177Q, 218-259 deletion Mutation：N7Q, N177Q, 218-259 deletion | P2E (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE；Blot Force 10, Blot Time 3.5 sec, 3 microL apply
|
Resolution 3.30 Å |
| 8GCM Cryo-EM Structure of the Prostaglandin E Receptor EP4 Coupled to G Protein Deposited 2023-03-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain R
1–488（488 aa）
|
Not recorded | YX9 (5S)-5-[(3R)-4,4-difluoro-3-hydroxy-4-phenylbutyl]-1-[6-(1H-tetrazol-5-yl)hexyl]pyrrolidin-2-one × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8GCP Cryo-EM Structure of the Prostaglandin E2 Receptor 4 Coupled to G Protein Deposited 2023-03-02 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain R
1–488（488 aa）
|
Not recorded | P2E (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8GD9 Cryo-EM Structure of the Prostaglandin E2 Receptor 4 Coupled to G Protein Deposited 2023-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain R
1–488（488 aa）
|
Not recorded | Z2C 4-({2-[(1R,2R,3R,5S)-3,5-dihydroxy-2-{(3S)-3-hydroxy-4-[3-(methoxymethyl)phenyl]butyl}cyclopentyl]ethyl}sulfanyl)butanoic acid × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 8GDA Cryo-EM Structure of the Prostaglandin E2 Receptor 4 Coupled to G Protein Deposited 2023-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain R
1–488（488 aa）
|
Not recorded | YZV (2S,5R)-5-[(1E,3S)-4,4-difluoro-3-hydroxy-4-phenylbut-1-en-1-yl]-1-[6-(1H-tetrazol-5-yl)hexyl]pyrrolidin-2-ol × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 8GDB Cryo-EM Structure of the Prostaglandin E2 Receptor 4 Coupled to G Protein Deposited 2023-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain R
1–488（488 aa）
|
Not recorded | P2E (Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9JQY Structural Insights into Selective Antagonism of TG6-129 and EP4 Prostaglandin Receptor Deposited 2024-09-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
2–366（365 aa）
|
Not recorded | A1EC5 ~{N}-[[4-[(5-ethyl-1,3,4-thiadiazol-2-yl)sulfamoyl]phenyl]carbamothioyl]-3-(4-fluorophenyl)propanamide × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.3
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.92 Å |
| 9JQZ Structural Insights into Selective Antagonism Grapiprant and EP4 Prostaglandin Receptor Deposited 2024-09-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
2–366（365 aa）
|
Mutation：T228A,N232Q,A287L,G331R,N402Q | A1ECR Grapiprant × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.3
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |