Current Protein Identity:P37344
New Search
Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2BJV Crystal Structure of PspF(1-275) R168A mutant Deposited 2005-02-08 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–265(265 aa)
Fragment:AAA DOMAIN, RESIDUES 1-265
|
Mutation:YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8;2 M AMMONIUM FORMATE, 0.1 MM HEPES PH 8.0, 10% MPD
|
Resolution 1.70 Å R-free 0.212 |
| 2BJW PspF AAA domain Deposited 2005-02-08 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–265(265 aa)
Fragment:AAA DOMAIN, RESIDUES 1-265
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8;2.0 M AMMONIUM FORMATE, 0.1 M HEPES PH 8.0, 10% MPD
|
Resolution 1.75 Å R-free 0.208 |
| 2C96 Structural basis of the nucleotide driven conformational changes in the AAA domain of transcription activator PspF Deposited 2005-12-09 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–265(265 aa)
Fragment:AAA DOMAIN, RESIDUES 1-265
|
Not recorded | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8;2M AMMONIUM FORMATE, 0.1 M HEPES PH 8.0, 5% MPD
|
Resolution 1.80 Å R-free 0.246 |
| 2C98 Structural basis of the nucleotide driven conformational changes in the AAA domain of transcription activator PspF Deposited 2005-12-09 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–265(265 aa)
Fragment:AAA DOMAIN, RESIDUES 1-265
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8;2M AMMONIUM FORMATE, 0.1 M HEPES PH 8.0, 5% MPD
|
Resolution 1.90 Å R-free 0.203 |
| 2C99 Structural basis of the nucleotide driven conformational changes in the AAA domain of transcription activator PspF Deposited 2005-12-09 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–265(265 aa)
Fragment:AAA DOMAIN, RESIDUES 1-265
|
Not recorded | ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8;2M AMMONIUM FORMATE, 0.1 M HEPES PH 8.0, 5% MPD
|
Resolution 1.90 Å R-free 0.212 |
| 2C9C Structural basis of the nucleotide driven conformational changes in the AAA domain of transcription activator PspF Deposited 2005-12-09 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–265(265 aa)
Fragment:AAA DOMAIN, RESIDUES 1-265
|
Mutation:YES | MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8;2M AMMONIUM FORMATE, 0.1 M HEPES PH 8.0, 5% MPD
|
Resolution 2.10 Å R-free 0.198 |
| 2VII PspF1-275-Mg-AMP Deposited 2007-12-04 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–259(259 aa)
Fragment:AAA DOMAIN, RESIDUES 1-259
|
Not recorded | AMP ADENOSINE MONOPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8;0.1M TRIS-HCL PH 8.0, 2.0M AMMONIUM FORMATE, 10% MPD
|
Resolution 2.85 Å R-free 0.241 |
| 4QNM CRYSTAL STRUCTURE of PSPF(1-265) E108Q MUTANT Deposited 2014-06-18 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–265(265 aa)
Fragment:Phage Shock protein F AAA DOMAIN, RESIDUES 1-265
|
Mutation:E108Q | EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8;292 K;100mM Bis-Tris pH 8.0, 12-16% MPD, 2M Ammonium formate, VAPOR DIFFUSION, SITTING DROP, temperature 292K
|
Resolution 1.63 Å R-free 0.203 |
| 4QNR CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP Deposited 2014-06-18 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–265(265 aa)
Fragment:Phage Shock protein F AAA DOMAIN, RESIDUES 1-265
|
Mutation:E108Q | ATP ADENOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8;292 K;100mM Bis-Tris pH 8.0, 12-16% MPD, 2M Ammonium formate , VAPOR DIFFUSION, SITTING DROP, temperature 292K
|
Resolution 1.54 Å R-free 0.178 |
| 4QOS CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ADP Deposited 2014-06-20 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
1–265(265 aa)
Fragment:Phage Shock protein F AAA DOMAIN, RESIDUES 1-265
|
Mutation:E108Q | ADP ADENOSINE-5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 8;292 K;100mM Bis-Tris pH 8.0, 12-16% MPD, 2M Ammonium formate, VAPOR DIFFUSION, SITTING DROP, temperature 292K
|
Resolution 1.42 Å R-free 0.190 |
| 5NSS Cryo-EM structure of RNA polymerase-sigma54 holoenzyme with promoter DNA and transcription activator PspF intermedate complex Deposited 2017-04-26 | Assembly 1 Insufficient information Heteromer;Protein × 12 PDB declaration: octameric(8) Review required |
Chain F
1–275(275 aa)
Chain G
1–275(275 aa)
Chain J
1–275(275 aa)
Chain K
1–275(275 aa)
Chain L
1–275(275 aa)
Chain N
1–275(275 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å |
| 9Q90 CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF Deposited 2025-02-26 | Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: 14-meric(14) Consistent with all polymers |
Chain 1
1–275(275 aa)
Chain 2
1–275(275 aa)
Chain 3
1–275(275 aa)
Chain 4
1–275(275 aa)
Chain 5
1–275(275 aa)
Chain 6
1–275(275 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 AF3 ALUMINUM FLUORIDE × 4 MG MAGNESIUM ION × 4 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9Q91 CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -11 to -8 - conformation 6 Deposited 2025-02-26 | Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: 14-meric(14) Consistent with all polymers |
Chain 1
1–259(259 aa)
Chain 2
1–259(259 aa)
Chain 3
1–259(259 aa)
Chain 4
1–259(259 aa)
Chain 5
1–259(259 aa)
Chain 6
1–259(259 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.20 Å |
| 9Q92 CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -11 to -8 - conformation 5 Deposited 2025-02-26 | Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: 14-meric(14) Consistent with all polymers |
Chain 1
1–259(259 aa)
Chain 2
1–259(259 aa)
Chain 3
1–259(259 aa)
Chain 4
1–259(259 aa)
Chain 5
1–259(259 aa)
Chain 6
1–259(259 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 AF3 ALUMINUM FLUORIDE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.80 Å |
| 9Q93 CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -11 to -8 - conformation 4 Deposited 2025-02-26 | Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: 14-meric(14) Consistent with all polymers |
Chain 1
1–259(259 aa)
Chain 2
1–259(259 aa)
Chain 3
1–259(259 aa)
Chain 4
1–259(259 aa)
Chain 5
1–259(259 aa)
Chain 6
1–259(259 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 AF3 ALUMINUM FLUORIDE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.60 Å |
| 9Q94 CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -11 to -8 - conformation 3 Deposited 2025-02-26 | Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: tetradecameric(14) Consistent with all polymers |
Chain 1
1–275(275 aa)
Chain 2
1–275(275 aa)
Chain 3
1–275(275 aa)
Chain 4
1–275(275 aa)
Chain 5
1–275(275 aa)
Chain 6
1–275(275 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 AF3 ALUMINUM FLUORIDE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å |
| 9Q95 CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -10 to -1 Deposited 2025-02-26 | Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: tetradecameric(14) Consistent with all polymers |
Chain 1
1–275(275 aa)
Chain 2
1–275(275 aa)
Chain 3
1–275(275 aa)
Chain 4
1–275(275 aa)
Chain 5
1–275(275 aa)
Chain 6
1–275(275 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 AF3 ALUMINUM FLUORIDE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.80 Å |
| 9Q97 CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -11 to -8 - conformation 2 Deposited 2025-02-26 | Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: tetradecameric(14) Consistent with all polymers |
Chain 1
1–259(259 aa)
Chain 2
1–259(259 aa)
Chain 3
1–259(259 aa)
Chain 4
1–259(259 aa)
Chain 5
1–259(259 aa)
Chain 6
1–259(259 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 AF3 ALUMINUM FLUORIDE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å |
| 9Q98 CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -11 to -8 - conformation 1 Deposited 2025-02-26 | Assembly 1 Protein–DNA Heteromer;Protein × 12 PDB declaration: tetradecameric(14) Consistent with all polymers |
Chain 1
1–259(259 aa)
Chain 2
1–259(259 aa)
Chain 3
1–259(259 aa)
Chain 4
1–259(259 aa)
Chain 5
1–259(259 aa)
Chain 6
1–259(259 aa)
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 5 AF3 ALUMINUM FLUORIDE × 5 MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.30 Å |