Current Protein Identity：P46531
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1PB5 NMR Structure of a Prototype LNR Module from Human Notch1 Deposited 2003-05-14 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1447–1481（35 aa）
Fragment：First LNR module
|
Not recorded | CA CALCIUM ION × 1 | SOLUTION NMR |
NMR measurement conditions
pH 6.5；298 K；Ionic strength (raw mmCIF value) 10mM Ca2+；Pressure ambient
NMR measurement conditions
pH 7；283 K；Ionic strength (raw mmCIF value) 10mM Ca2+, 50mM PIPES；Pressure ambient
NMR sample composition
1mM LNRA U-15N, 10mM Ca2+, 0.5mM DSS pH 6.5 | 90% H2O/10% D2O
NMR sample composition
1mM LNRA U-15N,13C, 10mM Ca2+, 0.5mM DSS pH 6.5 | 90% H2O/10% D2O
NMR sample composition
1.5mM LNRA U-15N,13C, 10 mM Ca2+, 0.5mM DSS, 50 mM deuterated PIPES pH 7.0 | 90% H2O/10% D2O
|
Resolution not provided |
| 1TOZ NMR structure of the human NOTCH-1 ligand binding region Deposited 2004-06-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
411–526（116 aa）
Fragment：NOTCH-1 EGF 11-13
|
Mutation：M477I | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.1；298 K；Ionic strength (raw mmCIF value) 15mM CaCl2；Pressure ambient
NMR sample composition
0.5-1mM protein | 90% H2O, 10% D2O, 15mM CaCl2, 0.02% NaN3
|
Resolution not provided |
| 1YYH Crystal structure of the human Notch 1 ankyrin domain Deposited 2005-02-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1873–2115（243 aa）
Fragment：ankyrin domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；ammonium hydrogen phosphate, sodium chloride, imidazole, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.90 Å R-free 0.193 |
| 1YYH Crystal structure of the human Notch 1 ankyrin domain Deposited 2005-02-25 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1873–2115（243 aa）
Fragment：ankyrin domain
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；298 K；ammonium hydrogen phosphate, sodium chloride, imidazole, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.90 Å R-free 0.193 |
| 2F8X Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence Deposited 2005-12-04 | Assembly 1 Protein–DNA Heteromer；Protein × 3 PDB declaration: pentameric（5） Consistent with all polymers |
Chain K
1873–2127（255 aa）
Fragment：[Contains: Notch 1 extracellular truncation; Notch 1 intracellular domain]
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
HANGING DROP；pH 7.9；298 K；50mM HEPES, 6% PEG 3350, and 5% ethylene glycol, pH 7.9, HANGING DROP, temperature 298K
|
Resolution 3.25 Å R-free 0.257 |
| 2F8Y Crystal structure of human Notch1 ankyrin repeats to 1.55A resolution. Deposited 2005-12-04 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1906–2127（222 aa）
Fragment：ankyrin repeat domain, repeats 1-7
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.55 Å R-free 0.187 |
| 2F8Y Crystal structure of human Notch1 ankyrin repeats to 1.55A resolution. Deposited 2005-12-04 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1906–2127（222 aa）
Fragment：ankyrin repeat domain, repeats 1-7
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.55 Å R-free 0.187 |
| 2HE0 Crystal structure of a human Notch1 ankyrin domain mutant Deposited 2006-06-21 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1873–2115（243 aa）
Fragment：Ankyrin Domain, residues 1873-2115
Chain B
1873–2115（243 aa）
Fragment：Ankyrin Domain, residues 1873-2115
|
Mutation：E58A, R66A Mutation：E58A, R66A | EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；298 K；0.55M Sodium/Potassium Tartrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.231 |
| 2VJ3 Human Notch-1 EGFs 11-13 Deposited 2007-12-06 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
411–526（116 aa）
Fragment：EGFS 11-13, RESIDUES 411-526
|
Not recorded | CA CALCIUM ION × 4 NA SODIUM ION × 2 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.7；30% (W/V) PEG-5000-MME, 100MM SODIUM ACETATE, PH5.7
|
Resolution 2.60 Å R-free 0.244 |
| 3ETO 2 Angstrom Xray structure of the NOTCH1 Negative Regulatory Region (NRR) Deposited 2008-10-08 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1447–1734（288 aa）
Fragment：NOTCH1 Negative Regulatory Region, residues 1447-1734
|
Not recorded | CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；0.1M Sodium acetate pH 4.0, 1.0-1.5M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.252 |
| 3ETO 2 Angstrom Xray structure of the NOTCH1 Negative Regulatory Region (NRR) Deposited 2008-10-08 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1447–1734（288 aa）
Fragment：NOTCH1 Negative Regulatory Region, residues 1447-1734
|
Not recorded | CA CALCIUM ION × 3 CL CHLORIDE ION × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；0.1M Sodium acetate pH 4.0, 1.0-1.5M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.00 Å R-free 0.252 |
| 3I08 Crystal structure of the S1-cleaved Notch1 Negative Regulatory Region (NRR) Deposited 2009-06-24 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1446–1664（219 aa）
Fragment：Notch1 NRR (Residues 1446-1665)
Chain B
1666–1734（69 aa）
Fragment：Notch1 NRR (Residues 1666-1734)
|
Not recorded | CA CALCIUM ION × 3 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；0.1M NaOAc, 2.0 M NaCl, 10% glycerol, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.286 |
| 3I08 Crystal structure of the S1-cleaved Notch1 Negative Regulatory Region (NRR) Deposited 2009-06-24 | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1446–1664（219 aa）
Fragment：Notch1 NRR (Residues 1446-1665)
Chain D
1666–1734（69 aa）
Fragment：Notch1 NRR (Residues 1666-1734)
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；0.1M NaOAc, 2.0 M NaCl, 10% glycerol, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.286 |
| 3I08 Crystal structure of the S1-cleaved Notch1 Negative Regulatory Region (NRR) Deposited 2009-06-24 | Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1446–1664（219 aa）
Fragment：Notch1 NRR (Residues 1446-1665)
Chain B
1666–1734（69 aa）
Fragment：Notch1 NRR (Residues 1666-1734)
Chain C
1446–1664（219 aa）
Fragment：Notch1 NRR (Residues 1446-1665)
Chain D
1666–1734（69 aa）
Fragment：Notch1 NRR (Residues 1666-1734)
|
Not recorded | CA CALCIUM ION × 6 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；298 K；0.1M NaOAc, 2.0 M NaCl, 10% glycerol, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.20 Å R-free 0.286 |
| 3L95 Crystal structure of the human Notch1 Negative Regulatory Region (NRR) bound to the fab fragment of an antagonist antibody Deposited 2010-01-04 | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain X
1448–1621（174 aa）
Fragment：Negative regulatory region (NRR1)
Chain X
1670–1728（59 aa）
Fragment：Negative regulatory region (NRR1)
Chain Y
1448–1621（174 aa）
Fragment：Negative regulatory region (NRR1)
Chain Y
1670–1728（59 aa）
Fragment：Negative regulatory region (NRR1)
|
Not recorded | SO4 SULFATE ION × 1 CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；292 K；Equal volumes of protein and well solutions. Protein: 6 mg/mL in 150 mM NaCl, 20 mM Bis-Tris pH 6.5. Well solution: 300 mM di-Ammonium Sulfate, 20% PEG 5000 MME, 100 mM Tris pH 7.5, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.19 Å R-free 0.271 |
| 3L95 Crystal structure of the human Notch1 Negative Regulatory Region (NRR) bound to the fab fragment of an antagonist antibody Deposited 2010-01-04 | Assembly 2 Other combination Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain X
1448–1621（174 aa）
Fragment：Negative regulatory region (NRR1)
Chain X
1670–1728（59 aa）
Fragment：Negative regulatory region (NRR1)
Chain Y
1448–1621（174 aa）
Fragment：Negative regulatory region (NRR1)
Chain Y
1670–1728（59 aa）
Fragment：Negative regulatory region (NRR1)
|
Not recorded | CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；292 K；Equal volumes of protein and well solutions. Protein: 6 mg/mL in 150 mM NaCl, 20 mM Bis-Tris pH 6.5. Well solution: 300 mM di-Ammonium Sulfate, 20% PEG 5000 MME, 100 mM Tris pH 7.5, VAPOR DIFFUSION, temperature 292K
|
Resolution 2.19 Å R-free 0.271 |
| 3NBN Crystal structure of a dimer of Notch Transcription Complex trimers on HES1 DNA Deposited 2010-06-03 | Assembly 1 Protein–DNA Heteromer；Protein × 6 PDB declaration: octameric（8） Consistent with all polymers |
Chain B
1872–2126（255 aa）
Fragment：residues 1872-2126
Chain E
1872–2126（255 aa）
Fragment：residues 1872-2126
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；298 K；3% PEG3350, 10% ethylene glycol, 0.15M NaCl, 0.1M magnesium chloride, 0.1M BIS-TRIS, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.45 Å R-free 0.298 |
| 3V79 Structure of human Notch1 transcription complex including CSL, RAM, ANK, and MAML-1 on HES-1 promoter DNA sequence Deposited 2011-12-20 | Assembly 1 Protein–DNA Heteromer；Protein × 4 PDB declaration: hexameric（6） Consistent with all polymers |
Chain K
1872–2126（255 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.9；298 K；6% PEG 3350, 5% Ethylene glycol, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 3.85 Å R-free 0.245 |
| 4CUD Human Notch1 EGF domains 11-13 mutant fucosylated at T466 Deposited 2014-03-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
410–526（117 aa）
Fragment：EGF 11-13, RESIDUES 410-526
|
Not recorded | FUC alpha-L-fucopyranose × 2 CA CALCIUM ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6；pH 6.0
|
Resolution 1.85 Å R-free 0.260 |
| 4CUE Human Notch1 EGF domains 11-13 mutant T466V Deposited 2014-03-18 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
411–526（116 aa）
Fragment：EGF 11-13, RESIDUES 411-526
|
Mutation：YES | CA CALCIUM ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.5；pH 5.5
|
Resolution 3.00 Å R-free 0.282 |
| 4CUF Human Notch1 EGF domains 11-13 mutant T466S Deposited 2014-03-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
411–526（116 aa）
Fragment：EGF 11-13, RESIDUES 411-526
|
Mutation：YES | CA CALCIUM ION × 3 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.29 Å R-free 0.286 |
| 4D0E Human Notch1 EGF domains 11-13 mutant GlcNAc-fucose disaccharide modified at T466 Deposited 2014-04-25 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
411–526（116 aa）
Fragment：EGF 11-13, RESIDUES 411-526
|
Not recorded | CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6；pH 6.0
|
Resolution 1.61 Å R-free 0.270 |
| 4D0F Human Notch1 EGF domains 11-13 mutant T466A Deposited 2014-04-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
411–526（116 aa）
Fragment：EGF 11-13
|
Mutation：YES | CA CALCIUM ION × 6 EDO 1,2-ETHANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 4.5；pH 4.5
|
Resolution 2.80 Å R-free 0.265 |
| 5FM9 human Notch 1, EGF 4-7 Deposited 2015-11-02 | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
140–294（155 aa）
Fragment：EGF DOMAINS 4-7, UNP RESIDUES 140-294
|
Not recorded | CA CALCIUM ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；PH 8.5
|
Resolution 2.92 Å R-free 0.237 |
| 5FMA human Notch 1, EGF 4-7 Deposited 2015-11-02 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
142–294（153 aa）
Fragment：EGF DOMAINS 4-7, RESIDUES 142-294
|
Not recorded | CA CALCIUM ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.46 Å R-free 0.263 |
| 5FMA human Notch 1, EGF 4-7 Deposited 2015-11-02 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
142–294（153 aa）
Fragment：EGF DOMAINS 4-7, RESIDUES 142-294
|
Not recorded | CA CALCIUM ION × 2 CL CHLORIDE ION × 2 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.46 Å R-free 0.263 |
| 5KZO Notch1 transmembrane and associated juxtamembrane segment Deposited 2016-07-25 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1721–1771（51 aa）
Fragment：UNP residues 1721-1771
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 5.5；318 K；Ionic strength (raw mmCIF value) 65 mM imidazole；Pressure 1
NMR sample composition
350 uM [U-99% 15N] Notch1, 150 mg/mL DHPC/DMPC bicelle, 1 mM EDTA, 65 mM imidazole, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
500 uM [U-99% 13C; U-99% 15N] Notch1, 150 mg/mL DHPC/DMPC bicelle, 100% D2O | 100% D2O
NMR sample composition
500 uM [U-99% 13C; U-99% 15N] Notch1, 150 mg/mL DHPC/DMPC, 1 mM EDTA, 65 mM imidazole, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5L0R human POGLUT1 in complex with Notch1 EGF12 and UDP Deposited 2016-07-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
452–491（40 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 UDP URIDINE-5'-DIPHOSPHATE × 1 GOL GLYCEROL × 1 CL CHLORIDE ION × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；20% PEG5000 MME, 50 mM MES pH 6.5, 2mM CaCl2, 250mM NaCl, 5% glycerol
|
Resolution 1.50 Å R-free 0.171 |
| 5UB5 human POGLUT1 in complex with human Notch1 EGF12 S458T mutant and UDP Deposited 2016-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
452–491（40 aa）
Fragment：UNP residues 452-491
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 UDP URIDINE-5'-DIPHOSPHATE × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；295 K；20% (v/v) PEG5000 MME, 50 mM MES pH 6.5, 2mM CaCl2, 250mM NaCl, 5% (v/v) 2-methyl-2,4-pentanediol
|
Resolution 2.09 Å R-free 0.193 |
| 6PY8 Crystal structure of the RBPJ-NOTCH1-NRARP ternary complex bound to DNA Deposited 2019-07-29 | Assembly 1 Protein–DNA Heteromer；Protein × 3 PDB declaration: pentameric（5） Consistent with all polymers |
Chain F
1759–2127（369 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.8；298 K；50 mM Hepes pH 6.8, 200 mM Sodium Fluoride, 18% PEG 3350
|
Resolution 3.75 Å R-free 0.315 |
| 6PY8 Crystal structure of the RBPJ-NOTCH1-NRARP ternary complex bound to DNA Deposited 2019-07-29 | Assembly 2 Protein–DNA Heteromer；Protein × 3 PDB declaration: pentameric（5） Consistent with all polymers |
Chain K
1759–2127（369 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.8；298 K；50 mM Hepes pH 6.8, 200 mM Sodium Fluoride, 18% PEG 3350
|
Resolution 3.75 Å R-free 0.315 |
| 8OR5 Solution NMR structure of Notch1 TMD Deposited 2023-04-13 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1734–1760（27 aa）
Fragment：Notch1 transmembrane domain
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；300 K；Pressure 1
NMR sample composition
500 uM Notch1 WT TMD, trifluoroethanol/water 80/20 | trifluoroethanol/water 80/20
|
Resolution not provided |
| 8ORY Solution NMR structure of Notch1 L1740-1743 TMD Deposited 2023-04-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1734–1757（24 aa）
|
Mutation：A1740L, A1741L, A1742L, A1743L | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；300 K；Pressure 1
NMR sample composition
500 uM Notch1 L1740-1743 TMD, trifluoroethanol/water 80/20 | trifluoroethanol/water 80/20
|
Resolution not provided |
| 8ORZ Solution NMR structure of Notch1 G1740-1743 TMD Deposited 2023-04-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1734–1757（24 aa）
Fragment：Notch1 transmembrane domain
|
Mutation：A1740G, A1741G, A1742G, A1743G | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.5；300 K；Pressure 1
NMR sample composition
500 uM Notch1 G1740-1743 TMD, trifluoroethanol/water 80/20 | trifluoroethanol/water 80/20
|
Resolution not provided |
| 9B3G Human Notch-1 EGFs 21-23 Deposited 2024-03-19 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
790–906（117 aa）
Fragment：Human Notch1 EGF domains 21-23
|
Not recorded | BA BARIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M Ammonium acetate
0.1 M BIS-Tris pH 5.5
25% (w/v) PEG3350
|
Resolution 1.55 Å R-free 0.218 |
| 9B3N Human Notch-1 EGFs 20-24 Deposited 2024-03-19 | Assembly 1 Other combination Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
753–944（192 aa）
Fragment：Human Notch1 EGFs 20-24
|
Not recorded | BGC beta-D-glucopyranose × 1 FUC alpha-L-fucopyranose × 4 CA CALCIUM ION × 4 EDO 1,2-ETHANEDIOL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；294 K；0.2 M Imidazole Malate pH 6
8% (w/v) PEG4K
|
Resolution 1.50 Å R-free 0.198 |