Current Protein Identity：P52565
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1CC0 CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX Deposited 1999-03-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–204（204 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；293 K；CRYSTALS GROWN BY VAPOR DIFFUSION IN A SITTING DROP USING EQUAL VOLUMES OF PROTEIN AND RESERVOIR. CRYSTALLIZATION OCCURED OVER A PERIOD OF SEVERAL DAYS AT 20 DEGREE CELSIUS. PROTEIN (15 MG/ML) WAS IN 25MM TRIS-HCL, PH=8.0, 100MM NACL, 5MM MGCL2. RESERVOIR CONTAINED: 51% SATURATED AMMONIUM SULFATE, 100 MM SODIUM ACETATE, PH=5.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 5.00 Å |
| 1CC0 CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX Deposited 1999-03-03 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain F
1–204（204 aa）
|
Not recorded | MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.3；293 K；CRYSTALS GROWN BY VAPOR DIFFUSION IN A SITTING DROP USING EQUAL VOLUMES OF PROTEIN AND RESERVOIR. CRYSTALLIZATION OCCURED OVER A PERIOD OF SEVERAL DAYS AT 20 DEGREE CELSIUS. PROTEIN (15 MG/ML) WAS IN 25MM TRIS-HCL, PH=8.0, 100MM NACL, 5MM MGCL2. RESERVOIR CONTAINED: 51% SATURATED AMMONIUM SULFATE, 100 MM SODIUM ACETATE, PH=5.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 5.00 Å |
| 1FSO CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI QUADRUPLE MUTANT Deposited 2000-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K135A, K138A, K141A, L196F | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；ammonium sulfate and MES buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.00 Å R-free 0.232 |
| 1FST CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI TRIPLE MUTANT Deposited 2000-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
24–204（181 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K135A, K138A, K141A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 3400, isopropanol, HEPES buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.70 Å R-free 0.261 |
| 1FST CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI TRIPLE MUTANT Deposited 2000-09-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
24–204（181 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K135A, K138A, K141A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG 3400, isopropanol, HEPES buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.70 Å R-free 0.261 |
| 1FT0 CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI K113A MUTANT Deposited 2000-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K113A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；ammonium sulfate, Na/K tartrate, and sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.260 |
| 1FT0 CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI K113A MUTANT Deposited 2000-09-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K113A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；ammonium sulfate, Na/K tartrate, and sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.260 |
| 1FT0 CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI K113A MUTANT Deposited 2000-09-11 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
Chain B
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K113A Mutation：K113A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；ammonium sulfate, Na/K tartrate, and sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.60 Å R-free 0.260 |
| 1FT3 CRYSTAL STRUCTURE OF TRUNCATED RHOGDI K141A MUTANT Deposited 2000-09-11 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K141A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；ammonium sulfate, Na/K tartrate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.260 |
| 1FT3 CRYSTAL STRUCTURE OF TRUNCATED RHOGDI K141A MUTANT Deposited 2000-09-11 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K141A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；ammonium sulfate, Na/K tartrate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.260 |
| 1FT3 CRYSTAL STRUCTURE OF TRUNCATED RHOGDI K141A MUTANT Deposited 2000-09-11 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
Chain B
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K141A Mutation：K141A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；ammonium sulfate, Na/K tartrate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.260 |
| 1FT3 CRYSTAL STRUCTURE OF TRUNCATED RHOGDI K141A MUTANT Deposited 2000-09-11 | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
67–204（138 aa）
Fragment：C-TERMINAL DOMAIN
|
Mutation：K141A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；ammonium sulfate, Na/K tartrate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.260 |
| 1HH4 Rac1-RhoGDI complex involved in NADPH oxidase activation Deposited 2000-12-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
1–204（204 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GER GERAN-8-YL GERAN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；HANGING DROP AT 20C WITH RESERVOIR: 30% PEG 4000, 100 MM NA CITRATE PH=5.6, 5 MM MGCL2, 200 MM AMMONIUM ACETATE, pH 5.60
|
Resolution 2.70 Å R-free 0.280 |
| 1HH4 Rac1-RhoGDI complex involved in NADPH oxidase activation Deposited 2000-12-20 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–204（204 aa）
|
Not recorded | GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GER GERAN-8-YL GERAN × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；293 K；HANGING DROP AT 20C WITH RESERVOIR: 30% PEG 4000, 100 MM NA CITRATE PH=5.6, 5 MM MGCL2, 200 MM AMMONIUM ACETATE, pH 5.60
|
Resolution 2.70 Å R-free 0.280 |
| 1KMT Crystal structure of RhoGDI Glu(154,155)Ala mutant Deposited 2001-12-17 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
64–204（141 aa）
|
Mutation：E154A, E155A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；294 K；PEG 4000, Tris-HCl, Lithium sulfate, methylpentane diol, pH 8.5, VAPOR DIFFUSION, temperature 294K
|
Resolution 1.30 Å R-free 0.195 |
| 1KMT Crystal structure of RhoGDI Glu(154,155)Ala mutant Deposited 2001-12-17 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
64–204（141 aa）
|
Mutation：E154A, E155A | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；294 K；PEG 4000, Tris-HCl, Lithium sulfate, methylpentane diol, pH 8.5, VAPOR DIFFUSION, temperature 294K
|
Resolution 1.30 Å R-free 0.195 |
| 1QVY Crystal structure of RhoGDI K(199,200)R double mutant Deposited 2003-08-29 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
67–204（138 aa）
Fragment：C-terminal domain
|
Mutation：K(199,200)R | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；294 K；30% PEG 400, 0.1 M Tris, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP,
temperature 294.0K
|
Resolution 1.60 Å R-free 0.210 |
| 1QVY Crystal structure of RhoGDI K(199,200)R double mutant Deposited 2003-08-29 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
67–204（138 aa）
Fragment：C-terminal domain
|
Mutation：K(199,200)R | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；294 K；30% PEG 400, 0.1 M Tris, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP,
temperature 294.0K
|
Resolution 1.60 Å R-free 0.210 |
| 1QVY Crystal structure of RhoGDI K(199,200)R double mutant Deposited 2003-08-29 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
67–204（138 aa）
Fragment：C-terminal domain
|
Mutation：K(199,200)R | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；294 K；30% PEG 400, 0.1 M Tris, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP,
temperature 294.0K
|
Resolution 1.60 Å R-free 0.210 |
| 1QVY Crystal structure of RhoGDI K(199,200)R double mutant Deposited 2003-08-29 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
67–204（138 aa）
Fragment：C-terminal domain
|
Mutation：K(199,200)R | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；294 K；30% PEG 400, 0.1 M Tris, 0.2 M lithium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP,
temperature 294.0K
|
Resolution 1.60 Å R-free 0.210 |
| 1RHO STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR Deposited 1996-10-12 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
59–203（145 aa）
|
Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；2 M NH4 SO4 0.1 M TRIS PH 8.5
|
Resolution 2.50 Å R-free 0.298 |
| 1RHO STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR Deposited 1996-10-12 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
59–203（145 aa）
|
Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；2 M NH4 SO4 0.1 M TRIS PH 8.5
|
Resolution 2.50 Å R-free 0.298 |
| 1RHO STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR Deposited 1996-10-12 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
59–203（145 aa）
|
Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；2 M NH4 SO4 0.1 M TRIS PH 8.5
|
Resolution 2.50 Å R-free 0.298 |
| 1RHO STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR Deposited 1996-10-12 | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
59–203（145 aa）
Chain C
59–203（145 aa）
|
Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；2 M NH4 SO4 0.1 M TRIS PH 8.5
|
Resolution 2.50 Å R-free 0.298 |
| 1RHO STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR Deposited 1996-10-12 | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
59–203（145 aa）
|
Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；2 M NH4 SO4 0.1 M TRIS PH 8.5
|
Resolution 2.50 Å R-free 0.298 |
| 1RHO STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR Deposited 1996-10-12 | Assembly 6 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
59–203（145 aa）
Chain C
59–203（145 aa）
|
Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 18 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；2 M NH4 SO4 0.1 M TRIS PH 8.5
|
Resolution 2.50 Å R-free 0.298 |
| 1RHO STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR Deposited 1996-10-12 | Assembly 7 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain B
59–203（145 aa）
|
Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 18 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；2 M NH4 SO4 0.1 M TRIS PH 8.5
|
Resolution 2.50 Å R-free 0.298 |
| 1RHO STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR Deposited 1996-10-12 | Assembly 8 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
59–203（145 aa）
|
Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；2 M NH4 SO4 0.1 M TRIS PH 8.5
|
Resolution 2.50 Å R-free 0.298 |
| 1RHO STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR Deposited 1996-10-12 | Assembly 9 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
59–203（145 aa）
Chain C
59–203（145 aa）
|
Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：TRYPSIN PROTEOLYSIS AT R58 Non-standard monomer：Yes (specific site not provided by mmCIF) | SO4 SULFATE ION × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；2 M NH4 SO4 0.1 M TRIS PH 8.5
|
Resolution 2.50 Å R-free 0.298 |
| 2BXW CRYSTAL STRUCTURE OF RHOGDI Lys(135,138,141)Tyr MUTANT Deposited 2005-07-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
67–204（138 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 67-204
|
Mutation：YES | FMT FORMIC ACID × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.88；4M NA FORMATE, pH 7.88
|
Resolution 2.40 Å R-free 0.222 |
| 2BXW CRYSTAL STRUCTURE OF RHOGDI Lys(135,138,141)Tyr MUTANT Deposited 2005-07-27 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
67–204（138 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 67-204
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.88；4M NA FORMATE, pH 7.88
|
Resolution 2.40 Å R-free 0.222 |
| 2JHS CRYSTAL STRUCTURE OF RHOGDI K135H,K138H,K141H MUTANT Deposited 2007-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | FLC CITRATE ANION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；1.4 M SODIUM CITRATE, 100 MM HEPES PH 7.5
|
Resolution 1.95 Å R-free 0.209 |
| 2JHT CRYSTAL STRUCTURE OF RHOGDI K135T,K138T,K141T MUTANT Deposited 2007-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | SO4 SULFATE ION × 1 LI LITHIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；35% PEG 4000 0.2 M LITHIUM SULFATE 0.1M TRIS PH 8.5
|
Resolution 1.88 Å R-free 0.259 |
| 2JHT CRYSTAL STRUCTURE OF RHOGDI K135T,K138T,K141T MUTANT Deposited 2007-02-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；35% PEG 4000 0.2 M LITHIUM SULFATE 0.1M TRIS PH 8.5
|
Resolution 1.88 Å R-free 0.259 |
| 2JHT CRYSTAL STRUCTURE OF RHOGDI K135T,K138T,K141T MUTANT Deposited 2007-02-23 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；35% PEG 4000 0.2 M LITHIUM SULFATE 0.1M TRIS PH 8.5
|
Resolution 1.88 Å R-free 0.259 |
| 2JHT CRYSTAL STRUCTURE OF RHOGDI K135T,K138T,K141T MUTANT Deposited 2007-02-23 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8.5；35% PEG 4000 0.2 M LITHIUM SULFATE 0.1M TRIS PH 8.5
|
Resolution 1.88 Å R-free 0.259 |
| 2JHU CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT Deposited 2007-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5
|
Resolution 1.65 Å R-free 0.233 |
| 2JHU CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT Deposited 2007-02-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5
|
Resolution 1.65 Å R-free 0.233 |
| 2JHV CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT Deposited 2007-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5
|
Resolution 2.07 Å R-free 0.257 |
| 2JHV CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT Deposited 2007-02-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5
|
Resolution 2.07 Å R-free 0.257 |
| 2JHV CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT Deposited 2007-02-23 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5
|
Resolution 2.07 Å R-free 0.257 |
| 2JHV CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT Deposited 2007-02-23 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5
|
Resolution 2.07 Å R-free 0.257 |
| 2JHV CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT Deposited 2007-02-23 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5
|
Resolution 2.07 Å R-free 0.257 |
| 2JHV CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT Deposited 2007-02-23 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain F
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6.5；2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5
|
Resolution 2.07 Å R-free 0.257 |
| 2JHW CRYSTAL STRUCTURE OF RHOGDI E155A, E157A MUTANT Deposited 2007-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；2M AMMONIUM SULFATE, pH 8.00
|
Resolution 2.50 Å R-free 0.261 |
| 2JHW CRYSTAL STRUCTURE OF RHOGDI E155A, E157A MUTANT Deposited 2007-02-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 8；2M AMMONIUM SULFATE, pH 8.00
|
Resolution 2.50 Å R-free 0.261 |
| 2JHX CRYSTAL STRUCTURE OF RHOGDI E155H, E157H MUTANT Deposited 2007-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；1.4 M SODIUM CITRATE 0.1 M HEPES PH 7.5
|
Resolution 1.60 Å R-free 0.219 |
| 2JHX CRYSTAL STRUCTURE OF RHOGDI E155H, E157H MUTANT Deposited 2007-02-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；1.4 M SODIUM CITRATE 0.1 M HEPES PH 7.5
|
Resolution 1.60 Å R-free 0.219 |
| 2JHY CRYSTAL STRUCTURE OF RHOGDI E155H, E157H MUTANT Deposited 2007-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；1.4 M SODIUM CITRATE 0.1 M HEPES PH 7.5
|
Resolution 1.90 Å R-free 0.250 |
| 2JHZ CRYSTAL STRUCTURE OF RHOGDI E155S, E157S MUTANT Deposited 2007-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；1.4 M SODIUM CITRATE 0.1 M HEPES PH 7.5
|
Resolution 2.20 Å R-free 0.228 |
| 2JHZ CRYSTAL STRUCTURE OF RHOGDI E155S, E157S MUTANT Deposited 2007-02-23 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；1.4 M SODIUM CITRATE 0.1 M HEPES PH 7.5
|
Resolution 2.20 Å R-free 0.228 |
| 2JI0 CRYSTAL STRUCTURE OF RHOGDI K138Y, K141Y MUTANT Deposited 2007-02-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
66–201（136 aa）
Fragment：ISOPRENYL-BINDING DOMAIN, RESIDUES 66-201
|
Mutation：YES | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 7.5；32% PEG 8000, 0.22M AMMONIUM SULFATE, 0.1 M SODIUM CACODYLATE PH6.5, pH 7.50
|
Resolution 2.10 Å R-free 0.259 |
| 2N80 p75NTR DD:RhoGDI Deposited 2015-09-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
31–204（174 aa）
Fragment：UNP residues 31-204
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.9；301 K；Ionic strength (raw mmCIF value) 10；Pressure ambient
NMR sample composition
0.5 mM [U-99% 13C; U-99% 15N] p75NTR DD-1, 1 mM [U-98% 2H] DTT-2, 10 mM [U-98% 2H] HEPES-3, 1 mM EDTA-4, 1 mM sodium azide-5, 2 mM RhoGDI-6, 95% H2O/5% D2O | 95% H2O/5% D2O
NMR sample composition
0.5 mM [U-99% 13C; U-99% 15N] RhoGDI-7, 2 mM p75NTR DD-8, 1 mM [U-98% 2H] DTT-9, 10 mM [U-98% 2H] HEPES-10, 1 mM EDTA-11, 1 mM sodium azide-12, 95% H2O/5% D2O | 95% H2O/5% D2O
|
Resolution not provided |
| 8X8T NMR structure of p75NTR juxtamembrane domain in complex with RhoGDI N-terminal domain containing a phosphorylation-mimicking S34D mutation Deposited 2023-11-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
2–60（59 aa）
|
Mutation：S34D | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 6.8；293 K；Ionic strength (raw mmCIF value) 10；Pressure 1
NMR sample composition
0.8 mM [U-13C; U-15N] RhoGDI-NTD, 2.4 mM NA p75NTR-JMD, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.8 mM [U-13C; U-15N] p75NTR-JMD, 2.4 mM NA RhoGDI-NTD, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |