SAXSdb — 蛋白结构检索 — SAXSdb

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当前蛋白身份：P55980 重新检索

本组结构的主要差异维度

构建体不同突变/修饰不同组装状态不同实验环境不同结构质量指标不同

差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。

相关结构差异明细

一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。

PDB 条目	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法	实验环境	结构质量
3IEC Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates 提交 2009-07-22	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric（2）与蛋白数一致	链 E 885–1005（121 aa）片段：UNP RESIDUES 885-1005	未记录	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；296 K；Initial crystals were grown by vapor diffusion using hanging drops formed from mixing a 2:1 volume ratio of the protein complex with an equilibration buffer consisting of 22.5-25% polyethylene glycol (PEG) molecular weight 3350 Da, 300 mM Li2SO4, and 100 mM Bis-Tris pH 5.8-6.2, at 23 C. Higher quality crystals were obtained by micro-seeding and addition of 5% PEG 400 as an additive. For cryoprotection crystals where transferred directly into a buffer with a 25% PEG 3350 Da, 5-10% PEG 400 Da, 300mM Li2SO4, 100mM Bis-Tris pH 5.8-6.2, 7.5% glycerol, and flash-cooled immediately afterward to -160 C. , VAPOR DIFFUSION, HANGING DROP, temperature 296K	分辨率 2.20 Å R-free 0.248
3IEC Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates 提交 2009-07-22	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric（2）与蛋白数一致	链 F 885–1005（121 aa）片段：UNP RESIDUES 885-1005	未记录	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；296 K；Initial crystals were grown by vapor diffusion using hanging drops formed from mixing a 2:1 volume ratio of the protein complex with an equilibration buffer consisting of 22.5-25% polyethylene glycol (PEG) molecular weight 3350 Da, 300 mM Li2SO4, and 100 mM Bis-Tris pH 5.8-6.2, at 23 C. Higher quality crystals were obtained by micro-seeding and addition of 5% PEG 400 as an additive. For cryoprotection crystals where transferred directly into a buffer with a 25% PEG 3350 Da, 5-10% PEG 400 Da, 300mM Li2SO4, 100mM Bis-Tris pH 5.8-6.2, 7.5% glycerol, and flash-cooled immediately afterward to -160 C. , VAPOR DIFFUSION, HANGING DROP, temperature 296K	分辨率 2.20 Å R-free 0.248
3IEC Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates 提交 2009-07-22	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric（2）与蛋白数一致	链 G 885–1005（121 aa）片段：UNP RESIDUES 885-1005	未记录	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；296 K；Initial crystals were grown by vapor diffusion using hanging drops formed from mixing a 2:1 volume ratio of the protein complex with an equilibration buffer consisting of 22.5-25% polyethylene glycol (PEG) molecular weight 3350 Da, 300 mM Li2SO4, and 100 mM Bis-Tris pH 5.8-6.2, at 23 C. Higher quality crystals were obtained by micro-seeding and addition of 5% PEG 400 as an additive. For cryoprotection crystals where transferred directly into a buffer with a 25% PEG 3350 Da, 5-10% PEG 400 Da, 300mM Li2SO4, 100mM Bis-Tris pH 5.8-6.2, 7.5% glycerol, and flash-cooled immediately afterward to -160 C. , VAPOR DIFFUSION, HANGING DROP, temperature 296K	分辨率 2.20 Å R-free 0.248
3IEC Helicobacter pylori CagA Inhibits PAR1/MARK Family Kinases by Mimicking Host Substrates 提交 2009-07-22	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric（2）与蛋白数一致	链 H 885–1005（121 aa）片段：UNP RESIDUES 885-1005	未记录	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；296 K；Initial crystals were grown by vapor diffusion using hanging drops formed from mixing a 2:1 volume ratio of the protein complex with an equilibration buffer consisting of 22.5-25% polyethylene glycol (PEG) molecular weight 3350 Da, 300 mM Li2SO4, and 100 mM Bis-Tris pH 5.8-6.2, at 23 C. Higher quality crystals were obtained by micro-seeding and addition of 5% PEG 400 as an additive. For cryoprotection crystals where transferred directly into a buffer with a 25% PEG 3350 Da, 5-10% PEG 400 Da, 300mM Li2SO4, 100mM Bis-Tris pH 5.8-6.2, 7.5% glycerol, and flash-cooled immediately afterward to -160 C. , VAPOR DIFFUSION, HANGING DROP, temperature 296K	分辨率 2.20 Å R-free 0.248
4DVY Crystal structure of the Helicobacter pylori CagA oncoprotein 提交 2012-02-23	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric（1）与蛋白数一致	链 P 1–876（876 aa）片段：UNP residues 1-876	突变：H131M/L274M	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；8% EtOH, 50mM Tris-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 3.30 Å R-free 0.248
4DVZ Crystal structure of the Helicobacter pylori CagA oncoprotein 提交 2012-02-23	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric（1）与蛋白数一致	链 A 261–829（569 aa）片段：UNP residues 261-829	未记录	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；7% EtOH, 50mM Tris-HCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K	分辨率 3.19 Å R-free 0.279
4G0H Crystal structure of the N-terminal domain of Helicobacter pylori CagA protein 提交 2012-07-09	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric（1）与蛋白数一致	链 A 1–884（884 aa）片段：UNP residues 1 to 884	未记录	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% Ethanol, Na Cacodylate 100mM pH7, NaI 15mM, VAPOR DIFFUSION, HANGING DROP, temperature 293K	分辨率 3.60 Å R-free 0.341
4IRV Structure of the Helicobacter pylori CagA Oncogene Bound to the Human Tumor Suppressor Apoptosis-stimulating Protein of p53-2 提交 2013-01-15	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric（2）与蛋白数一致	链 A 19–235（217 aa）	非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234
4IRV Structure of the Helicobacter pylori CagA Oncogene Bound to the Human Tumor Suppressor Apoptosis-stimulating Protein of p53-2 提交 2013-01-15	Assembly 2 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric（2）与蛋白数一致	链 B 19–235（217 aa）	非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234
4IRV Structure of the Helicobacter pylori CagA Oncogene Bound to the Human Tumor Suppressor Apoptosis-stimulating Protein of p53-2 提交 2013-01-15	Assembly 3 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric（2）与蛋白数一致	链 C 19–235（217 aa）	非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234
4IRV Structure of the Helicobacter pylori CagA Oncogene Bound to the Human Tumor Suppressor Apoptosis-stimulating Protein of p53-2 提交 2013-01-15	Assembly 4 蛋白异源复合物异源复合物；蛋白 × 2 PDB 声明：dimeric（2）与蛋白数一致	链 D 19–235（217 aa）	非标准单体：是（mmCIF未提供具体位点）	未记录非水小分子	X-RAY DIFFRACTION	X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.5；Crystals were grown by vapor diffusion at 25 degrees C using hanging drops formed from mixing a 2ul of the protein complex with 2ul of an equilibration buffer (21% polyethylene glycol (PEG) molecular weight 4 kDa, 200 mM Li2 SO4 , and 100 mM Tris pH 8.5) and 0.6ul of the Silver Bullets additive 43 (Hampton Research HR2-996-43). Significantly higher quality crystals were obtained from selenomethionine-substituted protein complexes and they were used for the final refinement, VAPOR DIFFUSION, HANGING DROP	分辨率 2.04 Å R-free 0.234