当前蛋白身份：P62656
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相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 提交 2013-05-22 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 19 PDB 声明：eicosameric（20） 与全部聚合物一致 |
链 N
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
|
分辨率 4.20 Å R-free 0.258 |
| 4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. 提交 2007-02-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：54-meric（54） 与全部聚合物一致 |
链 o
1–61（61 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 3.71 Å R-free 0.353 |
| 4V4J Interactions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome. 提交 2007-07-18 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：54-meric（54） 与全部聚合物一致 |
链 o
1–61（61 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K
|
分辨率 3.83 Å R-free 0.351 |
| 4V63 Structural basis for translation termination on the 70S ribosome. 提交 2008-05-16 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 AN
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
分辨率 3.21 Å R-free 0.319 |
| 4V63 Structural basis for translation termination on the 70S ribosome. 提交 2008-05-16 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 CN
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
分辨率 3.21 Å R-free 0.319 |
| 4V67 Crystal structure of a translation termination complex formed with release factor RF2. 提交 2008-10-27 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 AN
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
分辨率 3.00 Å R-free 0.316 |
| 4V67 Crystal structure of a translation termination complex formed with release factor RF2. 提交 2008-10-27 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 CN
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 801 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
分辨率 3.00 Å R-free 0.316 |
| 4V7P Recognition of the amber stop codon by release factor RF1. 提交 2010-04-29 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 AN
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 250 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
|
分辨率 3.62 Å R-free 0.291 |
| 4V7P Recognition of the amber stop codon by release factor RF1. 提交 2010-04-29 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 DN
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 160 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
|
分辨率 3.62 Å R-free 0.291 |
| 4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. 提交 2010-12-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 AN
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
|
分辨率 3.50 Å R-free 0.264 |
| 4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. 提交 2010-12-13 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 CN
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
|
分辨率 3.50 Å R-free 0.264 |
| 4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. 提交 2010-12-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 AN
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 595 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
|
分辨率 3.40 Å R-free 0.266 |
| 4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. 提交 2010-12-13 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 CN
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 599 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
|
分辨率 3.40 Å R-free 0.266 |
| 4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 AN
2–61（60 aa）
|
未记录 | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
|
分辨率 3.86 Å R-free 0.317 |
| 4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-23 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 CN
2–61（60 aa）
|
未记录 | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
|
分辨率 3.86 Å R-free 0.317 |
| 4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-24 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 AN
2–61（60 aa）
|
未记录 | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 3.50 Å R-free 0.309 |
| 4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-24 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 CN
2–61（60 aa）
|
未记录 | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 3.50 Å R-free 0.309 |
| 4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-24 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 AN
2–61（60 aa）
|
未记录 | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 3.50 Å R-free 0.328 |
| 4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-24 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 CN
2–61（60 aa）
|
未记录 | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 3.50 Å R-free 0.328 |
| 4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-25 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 55 PDB 声明：60-meric（60） 与全部聚合物一致 |
链 AN
2–61（60 aa）
|
未记录 | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 4.00 Å R-free 0.329 |
| 4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-25 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 55 PDB 声明：60-meric（60） 与全部聚合物一致 |
链 CN
2–61（60 aa）
|
未记录 | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 4.00 Å R-free 0.329 |
| 4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. 提交 2013-04-26 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 AN
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；pH 7.0
|
分辨率 3.40 Å R-free 0.268 |
| 4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. 提交 2013-04-26 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 CN
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7；pH 7.0
|
分辨率 3.40 Å R-free 0.268 |
| 4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. 提交 2013-06-12 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：54-meric（54） 与全部聚合物一致 |
链 BN
2–61（60 aa）
|
未记录 | BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 3.40 Å R-free 0.268 |
| 4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. 提交 2013-06-12 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：54-meric（54） 与全部聚合物一致 |
链 DN
2–61（60 aa）
|
未记录 | BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 3.40 Å R-free 0.268 |
| 4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. 提交 2014-07-02 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 55 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 AN
2–61（60 aa）
|
未记录 | GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.80 Å R-free 0.331 |
| 4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. 提交 2014-07-02 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 55 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 CN
2–61（60 aa）
|
未记录 | GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.80 Å R-free 0.331 |
| 4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. 提交 2014-10-21 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 5I
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 618 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.10 Å R-free 0.235 |
| 4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. 提交 2014-10-21 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 5A
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 513 ZN ZINC ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
|
分辨率 3.10 Å R-free 0.235 |
| 4XEJ IRES bound to bacterial Ribosome 提交 2014-12-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 AS14
2–61（60 aa）
|
未记录 | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates.
Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes
mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C.
Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I
(100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
|
分辨率 3.80 Å R-free 0.284 |
| 4XEJ IRES bound to bacterial Ribosome 提交 2014-12-23 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：53-meric（53） 与全部聚合物一致 |
链 BS14
2–61（60 aa）
|
未记录 | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates.
Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes
mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C.
Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I
(100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
|
分辨率 3.80 Å R-free 0.284 |
| 4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome 提交 2015-04-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55MERIC（55） 与全部聚合物一致 |
链 1n
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
分辨率 3.10 Å R-free 0.271 |
| 4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome 提交 2015-04-20 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55MERIC（55） 与全部聚合物一致 |
链 2n
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
分辨率 3.10 Å R-free 0.271 |
| 5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP 提交 2015-07-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 52 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 BR
2–61（60 aa）
|
未记录 | NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM玻璃化条件
冷冻剂 ETHANE；LIQUID ETHANE
|
分辨率 4.70 Å |
| 5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch 提交 2015-08-17 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 OA
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
|
分辨率 3.63 Å R-free 0.287 |
| 5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch 提交 2015-08-17 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 KC
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
|
分辨率 3.63 Å R-free 0.287 |
| 5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair 提交 2015-10-13 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 5I
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
分辨率 2.95 Å R-free 0.242 |
| 5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair 提交 2015-10-13 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 47 PDB 声明：55-meric（55） 待复核 |
链 5A
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
|
分辨率 2.95 Å R-free 0.242 |
| 5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon 提交 2016-03-31 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 WA
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
|
分辨率 3.10 Å R-free 0.256 |
| 5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon 提交 2016-03-31 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 BD
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
|
分辨率 3.10 Å R-free 0.256 |
| 5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 提交 2017-03-22 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：52-meric（52） 与全部聚合物一致 |
链 1n
1–61（61 aa）
|
未记录 | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
|
分辨率 3.25 Å R-free 0.270 |
| 5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 提交 2017-03-22 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：52-meric（52） 与全部聚合物一致 |
链 2n
1–61（61 aa）
|
未记录 | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
|
分辨率 3.25 Å R-free 0.270 |
| 6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome 提交 2017-09-27 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 WA
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.40 Å |
| 6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome 提交 2017-09-27 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 AD
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.40 Å |
| 6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome 提交 2017-11-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 51 PDB 声明：57-meric（57） 与全部聚合物一致 |
链 XA
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.40 Å |
| 6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome 提交 2017-11-20 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 51 PDB 声明：57-meric（57） 与全部聚合物一致 |
链 CD
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.40 Å |
| 6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome 提交 2017-11-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 VA
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 826 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.55 Å |
| 6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome 提交 2017-11-20 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 YC
1–61（61 aa）
|
未记录 | MG MAGNESIUM ION × 692 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.55 Å |
| 6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State 提交 2018-11-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 AS14
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.20 Å R-free 0.321 |
| 6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State 提交 2018-11-08 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 BS14
2–61（60 aa）
|
未记录 | MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.20 Å R-free 0.321 |