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4v4j

Interactions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome.

Method: X-RAY DIFFRACTION Dmax: 293.7 Å Quality: EXCELLENT
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1. Protein Identity and Related Structures Protein Identity & Related Structures

50S ribosomal protein L1

OrganismNot specified

UniProt Q72GV9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain A； UniProt 1–229 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain A 1–229（229 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BC 2–229（228 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DC 2–229（228 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BC 2–229（228 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DC 2–229（228 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BC 2–229（228 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DC 2–229（228 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BC 2–229（228 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DC 2–229（228 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AC 2–229（228 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AC 2–229（228 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 71 1–229（229 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 79 1–229（229 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
7A5F Structure of the stalled human mitoribosome with P- and E-site mt-tRNAs Deposited 2020-08-21 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 85 PDB declaration: 91-meric
Chain n 1–229（229 aa）
Not recorded MG MAGNESIUM ION × 128 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SO4 SULFATE ION × 1 MLI MALONATE ION × 3 CL CHLORIDE ION × 2 NA SODIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.40 Å
7A5G Structure of the elongating human mitoribosome bound to mtEF-Tu.GMPPCP and A/T mt-tRNA Deposited 2020-08-21 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 86 PDB declaration: 94-meric
Chain n 1–229（229 aa）
Not recorded MG MAGNESIUM ION × 128 SPD SPERMIDINE × 1 ZN ZINC ION × 7 GDP GUANOSINE-5'-DIPHOSPHATE × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 NA SODIUM ION × 4 SO4 SULFATE ION × 1 MLI MALONATE ION × 3 CL CHLORIDE ION × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.33 Å
7A5J Structure of the split human mitoribosomal large subunit with P-and E-site mt-tRNAs Deposited 2020-08-21 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 63-meric
Chain n 1–229（229 aa）
Not recorded MG MAGNESIUM ION × 92 ZN ZINC ION × 3 CL CHLORIDE ION × 2 NA SODIUM ION × 4 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å

11 other PDB entries and 16 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL1_THET2
Isoform —
PDB entities 3
Chains and sequence ranges Author chain A； PDBConstruct 1–229； UniProt 1–229

50S ribosomal protein L2

OrganismNot specified

UniProt Q72I07

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain B； UniProt 1–276 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain B 1–276（276 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BD 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DD 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BD 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DD 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BC 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CC 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BC 2–272（271 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DC 2–272（271 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BC 2–272（271 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DC 2–272（271 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BD 2–276（275 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DD 2–276（275 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BD 2–276（275 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DD 2–276（275 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BD 2–276（275 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DD 2–276（275 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BD 2–276（275 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DD 2–276（275 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BD 2–272（271 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DD 2–272（271 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AD 2–272（271 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CD 2–272（271 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BD 2–276（275 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DD 2–276（275 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 11 2–273（272 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 19 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 11 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 19 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 11 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 19 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 11 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 19 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL02 2–272（271 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL02 2–272（271 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1D 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2D 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AD 5–276（272 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AD 5–276（272 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain A 2–272（271 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain WA 2–272（271 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 11 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 19 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain E 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain JB 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1D 1–276（276 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2D 1–276（276 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain E 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain IB 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain E 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain JB 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain E 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain HB 2–276（275 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain BD 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain DD 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1D 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2D 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1D 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2D 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1D 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2D 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL02 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL02 1–276（276 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

33 other PDB entries and 63 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL2_THET2
Isoform —
PDB entities 4
Chains and sequence ranges Author chain B； PDBConstruct 1–276； UniProt 1–276

50S ribosomal protein L3

OrganismNot specified

UniProt Q72I04

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain C； UniProt 1–206 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain C 1–206（206 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BE 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DE 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BE 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DE 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BD 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CD 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BD 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DD 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BD 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DD 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BE 1–205（205 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DE 1–205（205 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BE 1–205（205 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DE 1–205（205 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BE 1–205（205 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DE 1–205（205 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BE 1–205（205 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DE 1–205（205 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BE 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DE 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AE 1–204（204 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CE 1–204（204 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BE 1–205（205 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DE 1–205（205 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 21 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 29 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL03 1–204（204 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL03 1–204（204 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1E 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2E 1–204（204 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AE 1–206（206 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AE 1–206（206 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain B 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain XA 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 21 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 29 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain F 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain KB 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1E 1–206（206 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2E 1–206（206 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain F 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain JB 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain F 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain KB 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain F 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain IB 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1E 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2E 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL03 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL03 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

27 other PDB entries and 51 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL3_THET2
Isoform —
PDB entities 5
Chains and sequence ranges Author chain C； PDBConstruct 1–206； UniProt 1–206

50S ribosomal protein L4

OrganismNot specified

UniProt Q72I05

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain D； UniProt 1–205 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain D 1–205（205 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BF 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DF 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BF 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DF 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BE 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CE 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BE 1–202（202 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DE 1–202（202 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BE 1–202（202 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DE 1–202（202 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BF 1–203（203 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DF 1–203（203 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BF 1–203（203 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DF 1–203（203 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BF 1–203（203 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DF 1–203（203 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BF 1–203（203 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DF 1–203（203 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BF 1–202（202 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DF 1–202（202 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AF 1–202（202 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CF 1–202（202 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL04 1–202（202 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL04 1–202（202 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain C 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain YA 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain G 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain LB 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain G 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain KB 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain G 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain LB 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain G 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain JB 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL04 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL04 1–205（205 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

19 other PDB entries and 37 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL4_THET2
Isoform —
PDB entities 6
Chains and sequence ranges Author chain D； PDBConstruct 1–205； UniProt 1–205

50S ribosomal protein L5

OrganismNot specified

UniProt Q72I16

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain E； UniProt 3–184 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain E 1–182（182 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BG 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DG 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BG 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DG 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BF 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CF 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BF 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DF 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BF 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DF 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BG 2–182（181 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DG 2–182（181 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BG 2–182（181 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DG 2–182（181 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BG 2–182（181 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DG 2–182（181 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BG 2–182（181 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DG 2–182（181 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BG 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DG 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AG 2–182（181 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CG 2–182（181 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL05 2–182（181 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL05 2–182（181 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain D 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain ZA 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain H 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain MB 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1G 1–182（182 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2G 1–182（182 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain H 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain LB 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain H 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain MB 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain H 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain KB 1–182（182 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL05 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL05 2–182（181 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

20 other PDB entries and 39 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL5_THET2
Isoform —
PDB entities 7
Chains and sequence ranges Author chain E； PDBConstruct 1–182； UniProt 3–184

50S ribosomal protein L6

OrganismNot specified

UniProt Q72I19

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain F； UniProt 1–180 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain F 1–180（180 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BH 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DH 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BH 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DH 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BG 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CG 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BG 12–170（159 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DG 12–170（159 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BG 12–170（159 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DG 12–170（159 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BH 12–178（167 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DH 12–178（167 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BH 12–178（167 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DH 12–178（167 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BH 12–178（167 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DH 12–178（167 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BH 12–178（167 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DH 12–178（167 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BH 12–170（159 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DH 12–170（159 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AH 12–170（159 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CH 12–170（159 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BH 12–178（167 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DH 12–178（167 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 51 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 59 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL06 12–170（159 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL06 12–170（159 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1H 2–175（174 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2H 2–175（174 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AH 4–177（174 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AH 4–177（174 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain E 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain AB 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 51 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 59 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain I 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain NB 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1H 1–180（180 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2H 1–180（180 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain I 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain MB 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain I 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain NB 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain I 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain LB 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL06 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL06 1–180（180 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

26 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL6_THET2
Isoform —
PDB entities 8
Chains and sequence ranges Author chain F； PDBConstruct 1–180； UniProt 1–180

50S ribosomal protein L9

OrganismNot specified

UniProt Q72GV5

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain G； UniProt 1–148 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain G 1–148（148 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V5E Insights into translational termination from the structure of RF2 bound to the ribosome Deposited 2009-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain DI 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 546 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；SEE SELMER ET AL., SCIENCE 2006, pH 7.1
Resolution 3.45 Å R-free 0.257
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BI 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DI 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BI 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DI 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BH 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CH 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BH 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DH 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BH 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DH 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BI 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DI 1–145（145 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AI 1–145（145 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CI 1–145（145 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL09 1–145（145 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL09 1–145（145 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain F 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain BB 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain J 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain OB 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1I 1–148（148 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2I 1–148（148 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain J 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain NB 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain J 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain OB 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain J 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain MB 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL09 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL09 1–148（148 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

17 other PDB entries and 32 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL9_THET2
Isoform —
PDB entities 9
Chains and sequence ranges Author chain G； PDBConstruct 1–148； UniProt 1–148

50S ribosomal protein L13

OrganismNot specified

UniProt Q72IN1

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain H； UniProt 1–140 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different construct Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain H 1–163（163 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BN 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DN 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BN 2–140（139 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DN 2–140（139 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BJ 2–140（139 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CJ 2–140（139 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BJ 2–138（137 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DJ 2–138（137 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BJ 2–138（137 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DJ 2–138（137 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BN 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DN 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BN 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DN 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BN 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DN 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BN 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DN 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BN 2–138（137 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DN 2–138（137 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AJ 2–138（137 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CJ 2–138（137 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BN 1–139（139 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DN 1–139（139 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 58 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 15 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 58 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 15 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 58 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 15 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 58 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 15 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL13 2–138（137 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL13 2–138（137 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1N 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2N 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AK 2–140（139 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AK 2–140（139 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain H 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain DB 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 58 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 15 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain K 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain PB 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1N 1–140（140 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2N 1–140（140 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5VPP The 70S P-site tRNA SufA6 complex Deposited 2017-05-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric
Chain RN 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 768 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
Resolution 3.90 Å
5VPP The 70S P-site tRNA SufA6 complex Deposited 2017-05-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric
Chain YN 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 547 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
Resolution 3.90 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain K 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain OB 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain K 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain PB 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain K 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain NB 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain BN 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain DN 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1N 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2N 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1N 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2N 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1N 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2N 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL13 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL13 1–140（140 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

34 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL13_THET2
Isoform —
PDB entities 10
Chains and sequence ranges Author chain H； PDBConstruct 1–140； UniProt 1–140

50S ribosomal protein L14

OrganismNot specified

UniProt Q72I14

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain I； UniProt 1–122 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain I 1–122（122 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BO 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DO 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BO 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DO 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BK 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CK 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BK 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DK 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BK 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DK 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BO 1–122（122 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DO 1–122（122 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BO 1–122（122 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DO 1–122（122 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BO 1–122（122 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DO 1–122（122 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BO 1–122（122 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DO 1–122（122 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BO 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DO 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AK 1–122（122 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CK 1–122（122 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL14 1–122（122 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL14 1–122（122 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AL 1–122（122 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AL 1–122（122 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain I 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain EB 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain L 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain QB 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1O 1–122（122 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2O 1–122（122 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain L 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain PB 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain L 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain QB 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain L 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain OB 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL14 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL14 1–122（122 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

22 other PDB entries and 41 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL14_THET2
Isoform —
PDB entities 11
Chains and sequence ranges Author chain I； PDBConstruct 1–122； UniProt 1–122

50S ribosomal protein L15

OrganismNot specified

UniProt Q72I23

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain J； UniProt 1–150 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain J 1–150（150 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BP 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DP 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BP 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DP 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BL 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CL 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL 5–150（146 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DL 5–150（146 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL 5–150（146 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DL 5–150（146 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BP 5–150（146 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DP 5–150（146 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BP 5–150（146 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DP 5–150（146 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BP 5–150（146 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DP 5–150（146 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BP 5–150（146 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DP 5–150（146 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BP 5–150（146 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DP 5–150（146 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AL 5–150（146 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CL 5–150（146 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BP 5–150（146 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DP 5–150（146 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 78 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 35 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL15 5–150（146 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL15 5–150（146 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1P 1–149（149 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2P 1–149（149 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AM 6–150（145 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AM 6–150（145 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain J 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain FB 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 78 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 35 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain M 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain RB 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1P 1–150（150 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2P 1–150（150 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain M 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain QB 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain M 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain RB 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain M 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain PB 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL15 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL15 1–150（150 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

26 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL15_THET2
Isoform —
PDB entities 12
Chains and sequence ranges Author chain J； PDBConstruct 1–150； UniProt 1–150

50S ribosomal protein L16

OrganismNot specified

UniProt Q72I11

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain K； UniProt 1–141 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain K 1–141（141 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BQ 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DQ 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BQ 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DQ 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BM 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CM 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BM 6–141（136 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DM 6–141（136 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BM 6–141（136 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DM 6–141（136 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BQ 1–141（141 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DQ 1–141（141 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BQ 1–141（141 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DQ 1–141（141 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BQ 1–141（141 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DQ 1–141（141 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BQ 1–141（141 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DQ 1–141（141 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BQ 6–139（134 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DQ 6–139（134 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AM 6–139（134 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CM 6–139（134 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BQ 1–141（141 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DQ 1–141（141 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL16 6–139（134 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL16 6–139（134 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain K 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain GB 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain N 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain SB 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1Q 1–141（141 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2Q 1–141（141 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain N 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain RB 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain N 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain SB 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain N 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain QB 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL16 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL16 1–141（141 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

21 other PDB entries and 41 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL16_THET2
Isoform —
PDB entities 13
Chains and sequence ranges Author chain K； PDBConstruct 1–141； UniProt 1–141

50S ribosomal protein L17

OrganismNot specified

UniProt Q72I33

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain L； UniProt 1–118 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain L 1–118（118 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BR 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DR 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BR 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DR 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BN 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CN 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BN 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DN 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BN 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DN 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BR 2–118（117 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DR 2–118（117 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BR 2–118（117 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DR 2–118（117 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BR 2–118（117 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DR 2–118（117 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BR 2–118（117 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DR 2–118（117 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BR 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DR 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AN 2–118（117 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CN 2–118（117 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BR 2–118（117 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DR 2–118（117 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 98 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 55 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 98 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 55 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 51 PDB declaration: 61-meric
Chain not uniquely mapped Reference range not declared
Not recorded PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME
Resolution 3.40 Å R-free 0.241
4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 51 PDB declaration: 62-meric
Chain DQ 2–118（117 aa）
Not recorded PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME
Resolution 3.40 Å R-free 0.241
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 98 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 55 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 98 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 55 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL17 2–118（117 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL17 2–118（117 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AO 2–118（117 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AO 2–118（117 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain L 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain HB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 98 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 55 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain O 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain TB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1R 1–118（118 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2R 1–118（118 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain O 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain SB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain O 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain TB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain O 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain RB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain BR 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain DR 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL17 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL17 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

34 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL17_THET2
Isoform —
PDB entities 14
Chains and sequence ranges Author chain L； PDBConstruct 1–118； UniProt 1–118

50S ribosomal protein L18

OrganismNot specified

UniProt Q72I20

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain M； UniProt 1–112 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain M 1–112（112 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BO 2–112（111 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CO 2–112（111 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BO 11–108（98 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DO 11–108（98 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BO 11–108（98 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DO 11–108（98 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BS 11–109（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DS 11–109（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BS 11–109（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DS 11–109（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BS 11–109（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DS 11–109（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BS 11–109（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DS 11–109（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BS 11–108（98 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DS 11–108（98 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AO 11–108（98 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CO 11–108（98 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BS 11–109（99 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DS 11–109（99 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain A8 2–112（111 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 65 2–112（111 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL18 11–108（98 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL18 11–108（98 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1S 3–112（110 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2S 3–112（110 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AP 3–112（110 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AP 3–112（110 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain M 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain IB 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain A8 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 65 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain P 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain UB 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1S 1–112（112 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2S 1–112（112 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain P 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain TB 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain P 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain UB 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain P 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain SB 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1S 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2S 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL18 2–112（111 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL18 2–112（111 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

27 other PDB entries and 51 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL18_THET2
Isoform —
PDB entities 15
Chains and sequence ranges Author chain M； PDBConstruct 1–112； UniProt 1–112

50S ribosomal protein L19

OrganismNot specified

UniProt Q72JU9

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain N； UniProt 1–146 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain N 1–146（146 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BT 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DT 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BT 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DT 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BP 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CP 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BP 1–137（137 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DP 1–137（137 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BP 1–137（137 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DP 1–137（137 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BT 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DT 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BT 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DT 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BT 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DT 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BT 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DT 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BT 1–137（137 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DT 1–137（137 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AP 1–137（137 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CP 1–137（137 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL19 1–137（137 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL19 1–137（137 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AQ 1–117（117 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AQ 1–117（117 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain N 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain JB 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Q 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain VB 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1T 1–146（146 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2T 1–146（146 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Q 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain UB 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain Q 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain VB 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Q 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain TB 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL19 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL19 1–146（146 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

22 other PDB entries and 41 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL19_THET2
Isoform —
PDB entities 16
Chains and sequence ranges Author chain N； PDBConstruct 1–146； UniProt 1–146

50S ribosomal protein L20

OrganismNot specified

UniProt Q72L76

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain O； UniProt 1–118 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain O 1–118（118 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BU 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DU 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BU 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DU 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BQ 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CQ 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BQ 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DQ 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BQ 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DQ 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BU 2–118（117 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DU 2–118（117 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BU 2–118（117 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DU 2–118（117 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BU 2–118（117 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DU 2–118（117 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BU 2–118（117 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DU 2–118（117 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BU 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DU 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AQ 2–118（117 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CQ 2–118（117 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BU 2–118（117 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DU 2–118（117 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain C8 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 85 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain C8 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 85 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain C8 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 85 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain C8 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 85 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL20 2–118（117 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL20 2–118（117 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1U 2–117（116 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2U 2–117（116 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AR 2–118（117 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AR 2–118（117 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain O 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain KB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain C8 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 85 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain WB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1U 1–118（118 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2U 1–118（118 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain VB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain WB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain R 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain UB 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain BU 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain DU 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1U 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2U 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1U 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2U 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1U 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2U 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL20 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL20 1–118（118 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

33 other PDB entries and 63 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL20_THET2
Isoform —
PDB entities 17
Chains and sequence ranges Author chain O； PDBConstruct 1–118； UniProt 1–118

50S ribosomal protein L21

OrganismNot specified

UniProt Q72HR2

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain P； UniProt 1–101 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain P 1–101（101 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BV 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DV 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BV 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DV 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BR 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CR 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BR 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DR 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BR 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DR 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BV 1–101（101 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DV 1–101（101 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BV 1–101（101 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DV 1–101（101 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BV 1–101（101 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DV 1–101（101 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BV 1–101（101 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DV 1–101（101 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BV 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DV 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AR 1–101（101 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CR 1–101（101 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BV 1–101（101 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DV 1–101（101 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain D8 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 95 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain D8 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 95 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain D8 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 95 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain D8 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 95 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL21 1–101（101 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL21 1–101（101 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1V 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2V 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AS 1–101（101 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AS 1–101（101 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain P 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain LB 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain D8 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 95 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain S 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain XB 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1V 1–101（101 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2V 1–101（101 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain S 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain WB 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain S 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain XB 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain S 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain VB 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain BV 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain DV 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1V 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2V 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1V 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2V 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1V 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2V 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL21 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL21 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

33 other PDB entries and 63 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL21_THET2
Isoform —
PDB entities 18
Chains and sequence ranges Author chain P； PDBConstruct 1–101； UniProt 1–101

50S ribosomal protein L22

OrganismNot specified

UniProt Q72I09

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain Q； UniProt 1–113 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain Q 1–113（113 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BW 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DW 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BW 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DW 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DS 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BW 1–113（113 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DW 1–113（113 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BW 1–113（113 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DW 1–113（113 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BW 1–113（113 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DW 1–113（113 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BW 1–113（113 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DW 1–113（113 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BW 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DW 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AS 1–112（112 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CS 1–112（112 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BW 1–113（113 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DW 1–113（113 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain E8 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain A5 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL22 1–112（112 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL22 1–112（112 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1W 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2W 1–112（112 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AT 1–110（110 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AT 1–110（110 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain Q 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain MB 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain E8 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain A5 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain T 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain YB 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1W 1–113（113 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2W 1–113（113 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain T 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain XB 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain T 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain YB 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain T 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain WB 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL22 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL22 1–113（113 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

26 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL22_THET2
Isoform —
PDB entities 19
Chains and sequence ranges Author chain Q； PDBConstruct 1–113； UniProt 1–113

50S ribosomal protein L23

OrganismNot specified

UniProt Q72I06

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain R； UniProt 1–96 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain R 1–96（96 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BX 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DX 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BX 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DX 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BT 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CT 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BT 3–94（92 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DT 3–94（92 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BT 3–94（92 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DT 3–94（92 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BX 3–95（93 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DX 3–95（93 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BX 3–95（93 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DX 3–95（93 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BX 3–95（93 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DX 3–95（93 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BX 3–95（93 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DX 3–95（93 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BX 3–94（92 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DX 3–94（92 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AT 3–94（92 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CT 3–94（92 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BX 3–95（93 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DX 3–95（93 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain F8 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain B5 1–94（94 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL23 3–94（92 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL23 3–94（92 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1X 1–95（95 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2X 1–95（95 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AU 3–96（94 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AU 3–96（94 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain R 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain NB 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain F8 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain B5 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain U 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ZB 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1X 1–96（96 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2X 1–96（96 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain U 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain YB 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain U 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain ZB 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain U 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain XB 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL23 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL23 1–96（96 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

26 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q72I06_THET2
Isoform —
PDB entities 20
Chains and sequence ranges Author chain R； PDBConstruct 1–96； UniProt 1–96

50S ribosomal protein L24

OrganismNot specified

UniProt Q72I15

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain S； UniProt 1–110 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain RY 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 325 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.278
4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain YY 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 351 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.278
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain S 1–110（110 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BY 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DY 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BY 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DY 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BU 2–110（109 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CU 2–110（109 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BU 2–101（100 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DU 2–101（100 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BU 2–101（100 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DU 2–101（100 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BY 2–108（107 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DY 2–108（107 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BY 2–108（107 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DY 2–108（107 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BY 2–108（107 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DY 2–108（107 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BY 2–108（107 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DY 2–108（107 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BY 2–101（100 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DY 2–101（100 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AU 2–101（100 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CU 2–101（100 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BY 2–108（107 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DY 2–108（107 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain G8 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain C5 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL24 2–101（100 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL24 2–101（100 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1Y 1–107（107 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2Y 1–107（107 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AV 1–110（110 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AV 1–110（110 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain S 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain OB 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain G8 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain C5 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain V 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AC 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1Y 1–110（110 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2Y 1–110（110 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain V 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ZB 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain V 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain AC 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain V 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain YB 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1Y 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2Y 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL24 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL24 1–110（110 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

28 other PDB entries and 53 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL24_THET2
Isoform —
PDB entities 21
Chains and sequence ranges Author chain S； PDBConstruct 1–110； UniProt 1–110

50S ribosomal protein L25

OrganismNot specified

UniProt Q72IA7

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain T； UniProt 1–206 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain T 1–206（206 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BZ 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DZ 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BZ 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DZ 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BV 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CV 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BV 3–190（188 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DV 3–190（188 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BV 3–190（188 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DV 3–190（188 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BZ 3–187（185 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DZ 3–187（185 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BZ 3–187（185 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DZ 3–187（185 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain BZ 3–187（185 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain DZ 3–187（185 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain BZ 3–187（185 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain DZ 3–187（185 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BZ 3–189（187 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DZ 3–189（187 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AV 3–189（187 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CV 3–189（187 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain BZ 3–187（185 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain DZ 3–187（185 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain H8 1–175（175 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain D5 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain H8 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain D5 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain H8 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain D5 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain H8 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain D5 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL25 3–189（187 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL25 3–189（187 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1Z 1–203（203 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2Z 1–203（203 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AW 1–180（180 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AW 1–180（180 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain T 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain PB 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain H8 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain D5 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain W 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BC 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1Z 1–206（206 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2Z 1–206（206 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain W 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AC 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain W 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain BC 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain W 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ZB 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain BZ 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain DZ 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL25 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL25 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 632 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 524 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 710 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2Z 1–206（206 aa）
Not recorded MG MAGNESIUM ION × 629 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279

35 other PDB entries and 67 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL25_THET2
Isoform —
PDB entities 22
Chains and sequence ranges Author chain T； PDBConstruct 1–206； UniProt 1–206

50S ribosomal protein L27

OrganismNot specified

UniProt Q72HR3

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain U； UniProt 1–85 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain U 1–85（85 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B0 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D0 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B0 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D0 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BW 2–85（84 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CW 2–85（84 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BW 10–85（76 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DW 10–85（76 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BW 10–85（76 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DW 10–85（76 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B0 2–85（84 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D0 2–85（84 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B0 2–85（84 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D0 2–85（84 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B0 2–85（84 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D0 2–85（84 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain B0 2–85（84 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain D0 2–85（84 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B0 10–85（76 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain D0 10–85（76 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AW 10–85（76 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CW 10–85（76 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL27 10–85（76 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL27 10–85（76 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain U 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain QB 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 10 1–85（85 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 20 1–85（85 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain X 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BC 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain X 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain CC 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain X 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AC 1–85（85 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å

18 other PDB entries and 35 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL27_THET2
Isoform —
PDB entities 23
Chains and sequence ranges Author chain U； PDBConstruct 1–85； UniProt 1–85

LSU ribosomal protein L28P

OrganismNot specified

UniProt Q72G84

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain V； UniProt 1–98 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain V 1–98（98 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B1 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D1 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B1 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D1 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BX 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CX 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BX 8–95（88 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DX 8–95（88 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BX 8–95（88 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DX 8–95（88 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B1 3–95（93 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D1 3–95（93 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B1 3–95（93 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D1 3–95（93 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B1 3–95（93 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D1 3–95（93 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain B1 3–95（93 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain D1 3–95（93 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B1 8–95（88 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain D1 8–95（88 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AX 8–95（88 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CX 8–95（88 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 51 PDB declaration: 61-meric
Chain CH 3–95（93 aa）
Not recorded PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME
Resolution 3.40 Å R-free 0.241
4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 51 PDB declaration: 62-meric
Chain not uniquely mapped Reference range not declared
Not recorded PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME
Resolution 3.40 Å R-free 0.241
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL28 8–95（88 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL28 8–95（88 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain V 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain RB 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Y 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DC 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 11 1–98（98 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 21 1–98（98 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Y 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CC 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain Y 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain DC 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Y 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BC 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL28 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL28 1–98（98 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

21 other PDB entries and 41 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q72G84_THET2
Isoform —
PDB entities 24
Chains and sequence ranges Author chain V； PDBConstruct 1–98； UniProt 1–98

50S ribosomal protein L29

OrganismNot specified

UniProt Q72I12

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain W； UniProt 1–72 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain W 1–72（72 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B2 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D2 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B2 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D2 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BY 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CY 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BY 1–62（62 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DY 1–62（62 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BY 1–62（62 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DY 1–62（62 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B2 2–72（71 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D2 2–72（71 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B2 2–72（71 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D2 2–72（71 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B2 2–72（71 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D2 2–72（71 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain B2 2–72（71 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain D2 2–72（71 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B2 1–62（62 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain D2 1–62（62 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AY 1–62（62 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CY 1–62（62 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain B2 2–72（71 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain D2 2–72（71 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain K8 4–69（66 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain G5 4–69（66 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain K8 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain G5 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain K8 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain G5 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain K8 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain G5 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL29 1–62（62 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL29 1–62（62 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 12 1–70（70 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 22 1–70（70 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain W 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain SB 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain K8 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain G5 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Z 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain EC 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 12 1–72（72 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 22 1–72（72 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Z 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DC 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain Z 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain EC 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Z 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CC 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain B2 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain D2 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 12 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 22 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 12 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 22 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 12 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 22 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL29 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL29 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 12 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 632 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 22 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 524 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 12 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 710 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 22 1–72（72 aa）
Not recorded MG MAGNESIUM ION × 629 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279

33 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL29_THET2
Isoform —
PDB entities 25
Chains and sequence ranges Author chain W； PDBConstruct 1–72； UniProt 1–72

LSU ribosomal protein L30P

OrganismNot specified

UniProt Q72I22

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain X； UniProt 1–60 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain X 1–60（60 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B3 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D3 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B3 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D3 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain BZ 1–59（59 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CZ 1–59（59 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BZ 1–59（59 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DZ 1–59（59 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BZ 1–59（59 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain DZ 1–59（59 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B3 1–60（60 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D3 1–60（60 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B3 1–60（60 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D3 1–60（60 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B3 1–60（60 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D3 1–60（60 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain B3 1–60（60 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain D3 1–60（60 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B3 1–59（59 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain D3 1–59（59 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain AZ 1–59（59 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain CZ 1–59（59 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain B3 1–60（60 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain D3 1–60（60 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain L8 2–60（59 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain H5 2–60（59 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain L8 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain H5 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL30 1–59（59 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL30 1–59（59 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 13 2–60（59 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 23 2–60（59 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain AZ 2–60（59 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AZ 2–60（59 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain X 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain TB 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain L8 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain H5 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5EL6 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position and antibiotic paromomycin Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain L8 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 593 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.10 Å R-free 0.249
5EL6 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position and antibiotic paromomycin Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain H5 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 480 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.10 Å R-free 0.249
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AA 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain FC 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 13 1–60（60 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 23 1–60（60 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AA 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain EC 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain AA 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain FC 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AA 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DC 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL30 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL30 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

28 other PDB entries and 53 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name Q72I22_THET2
Isoform —
PDB entities 26
Chains and sequence ranges Author chain X； PDBConstruct 1–60； UniProt 1–60

50S ribosomal protein L32

OrganismNot specified

UniProt P62652

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain Y； UniProt 1–60 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain Y 1–60（60 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B5 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D5 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B5 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D5 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B2 2–60（59 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain C2 2–60（59 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain B2 2–53（52 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain D2 2–53（52 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain B2 2–53（52 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain D2 2–53（52 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B5 2–60（59 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D5 2–60（59 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B5 2–60（59 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D5 2–60（59 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B5 2–60（59 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D5 2–60（59 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain B5 2–60（59 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain D5 2–60（59 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B5 2–53（52 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain D5 2–53（52 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain A2 2–53（52 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain C2 2–53（52 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL32 2–53（52 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL32 2–53（52 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain Y 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain UB 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CA 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain HC 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 15 1–60（60 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 25 1–60（60 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CA 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain GC 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain CA 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain HC 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CA 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain FC 1–60（60 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å

19 other PDB entries and 37 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL32_THET2
Isoform —
PDB entities 27
Chains and sequence ranges Author chain Y； PDBConstruct 1–60； UniProt 1–60

50S ribosomal protein L34

OrganismNot specified

UniProt P80340

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain Z； UniProt 1–49 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
10PX Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with benzoxaborole derivative of azithromycin (AZI-BB2), mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2026-02-01 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 98 PDB declaration: 112-meric
Chain 17 1–49（49 aa）
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 2794 K POTASSIUM ION × 2 A1C9N AZI-BB2 × 2 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.45 Å R-free 0.300
1VVJ Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome Deposited 2013-05-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 286 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.44 Å R-free 0.285
1VVJ Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome Deposited 2013-05-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 315 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.44 Å R-free 0.285
1VY4 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing acylated tRNA-substrates in the A and P sites. Deposited 2014-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1159 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.60 Å R-free 0.264
1VY4 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing acylated tRNA-substrates in the A and P sites. Deposited 2014-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 889 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.60 Å R-free 0.264
1VY5 Crystal structure of the Thermus thermophilus 70S ribosome in the post-catalysis state of peptide bond formation containing dipeptydil-tRNA in the A site and deacylated tRNA in the P site. Deposited 2014-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1165 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.55 Å R-free 0.280
1VY5 Crystal structure of the Thermus thermophilus 70S ribosome in the post-catalysis state of peptide bond formation containing dipeptydil-tRNA in the A site and deacylated tRNA in the P site. Deposited 2014-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 912 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.55 Å R-free 0.280
1VY6 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Puromycin in the A site and acylated tRNA in the P site. Deposited 2014-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 965 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.90 Å R-free 0.286
1VY6 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Puromycin in the A site and acylated tRNA in the P site. Deposited 2014-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 799 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.90 Å R-free 0.286
1VY7 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Cytidine-Puromycin in the A site and acylated tRNA in the P site. Deposited 2014-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1055 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.80 Å R-free 0.280
1VY7 Crystal structure of the Thermus thermophilus 70S ribosome in the pre-attack state of peptide bond formation containing short substrate-mimic Cytidine-Cytidine-Puromycin in the A site and acylated tRNA in the P site. Deposited 2014-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 845 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.80 Å R-free 0.280
4L47 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome Deposited 2013-06-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 325 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.22 Å R-free 0.260
4L47 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome Deposited 2013-06-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 354 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.22 Å R-free 0.260
4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 325 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.278
4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 351 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.278
4LEL Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCG-G on the Ribosome Deposited 2013-06-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 321 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.298
4LEL Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCG-G on the Ribosome Deposited 2013-06-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 349 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.298
4LFZ Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U in the Absence of Paromomycin Deposited 2013-06-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 320 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.92 Å R-free 0.265
4LFZ Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U in the Absence of Paromomycin Deposited 2013-06-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 354 ZN ZINC ION × 3 AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.92 Å R-free 0.265
4LNT Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-U on the Ribosome Deposited 2013-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 325 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
Resolution 2.94 Å R-free 0.265
4LNT Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-U on the Ribosome Deposited 2013-07-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 352 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
Resolution 2.94 Å R-free 0.265
4LSK Crystal Structure of tRNA Proline (CGG) Bound to Codon CCG-G on the Ribosome Deposited 2013-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 321 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.48 Å R-free 0.270
4LSK Crystal Structure of tRNA Proline (CGG) Bound to Codon CCG-G on the Ribosome Deposited 2013-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 351 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.48 Å R-free 0.270
4LT8 Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-G on the Ribosome Deposited 2013-07-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 324 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.14 Å R-free 0.262
4LT8 Crystal Structure of tRNA Proline (CGG) Bound to Codon CCC-G on the Ribosome Deposited 2013-07-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 352 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.14 Å R-free 0.262
4P6F Crystal structure of the peptolide 12C bound to bacterial ribosome Deposited 2014-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 328 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2
Resolution 3.60 Å R-free 0.254
4P6F Crystal structure of the peptolide 12C bound to bacterial ribosome Deposited 2014-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 368 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M Tris-Acetate, 0.2M KSCN, 10 mM MgCl2
Resolution 3.60 Å R-free 0.254
4P70 Crystal Structure of Unmodified tRNA Proline (CGG) Bound to Codon CCG on the Ribosome Deposited 2014-03-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 338 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.68 Å R-free 0.272
4P70 Crystal Structure of Unmodified tRNA Proline (CGG) Bound to Codon CCG on the Ribosome Deposited 2014-03-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 362 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.68 Å R-free 0.272
4TUA Crystal structure of ASL-Thr bound to Codon ACC-A on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 425 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.60 Å R-free 0.252
4TUA Crystal structure of ASL-Thr bound to Codon ACC-A on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 471 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.60 Å R-free 0.252
4TUB Crystal structure of tRNA-Thr bound to Codon ACC-C on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 426 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.60 Å R-free 0.259
4TUB Crystal structure of tRNA-Thr bound to Codon ACC-C on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 492 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.60 Å R-free 0.259
4TUC Crystal structure of ASL-SufJ bound to Codon ACC-A on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 354 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.60 Å R-free 0.253
4TUC Crystal structure of ASL-SufJ bound to Codon ACC-A on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 394 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.60 Å R-free 0.253
4TUD Crystal structure of ASL-SufJ bound to Codon ACC-C on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 393 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.60 Å R-free 0.244
4TUD Crystal structure of ASL-SufJ bound to Codon ACC-C on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 418 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.60 Å R-free 0.244
4TUE Crystal structure of ASL-SufJ bound to Codon ACC-U on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 422 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.50 Å R-free 0.250
4TUE Crystal structure of ASL-SufJ bound to Codon ACC-U on the Ribosome Deposited 2014-06-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 463 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl
Resolution 3.50 Å R-free 0.250
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain Z 1–49（49 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V4X Crystal structure of the 70S Thermus thermophilus ribosome showing how the 16S 3'-end mimicks mRNA E and P codons. Deposited 2006-06-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B6 1–49（49 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 5.00 Å R-free 0.323
4V4Y Crystal structure of the 70S Thermus thermophilus ribosome with translocated and rotated Shine-Dalgarno Duplex. Deposited 2006-06-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain B6 1–49（49 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 5.50 Å R-free 0.326
4V4Z 70S Thermus thermophilous ribosome functional complex with mRNA and E- and P-site tRNAs at 4.5A. Deposited 2006-06-27 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B6 1–49（49 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 4.51 Å R-free 0.345
4V51 Structure of the Thermus thermophilus 70S ribosome complexed with mRNA, tRNA and paromomycin Deposited 2006-07-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；0.2M KSCN, 4% PEG 20K, 4% PEG550 MME, 0.1M TRIS-ACETATE PH 7
Resolution 2.80 Å R-free 0.313
4V51 Structure of the Thermus thermophilus 70S ribosome complexed with mRNA, tRNA and paromomycin Deposited 2006-07-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 638 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；0.2M KSCN, 4% PEG 20K, 4% PEG550 MME, 0.1M TRIS-ACETATE PH 7
Resolution 2.80 Å R-free 0.313
4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 741 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
Resolution 3.50 Å R-free 0.326
4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 741 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
Resolution 3.50 Å R-free 0.326
4V5C Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A-site tRNA, deacylated P-site tRNA, and E-site tRNA. Deposited 2009-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；pH 7.1
Resolution 3.30 Å R-free 0.272
4V5C Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A-site tRNA, deacylated P-site tRNA, and E-site tRNA. Deposited 2009-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；pH 7.1
Resolution 3.30 Å R-free 0.272
4V5D Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA. Deposited 2009-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 673 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；pH 7.1
Resolution 3.50 Å R-free 0.256
4V5D Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA. Deposited 2009-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 673 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；pH 7.1
Resolution 3.50 Å R-free 0.256
4V5E Insights into translational termination from the structure of RF2 bound to the ribosome Deposited 2009-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 546 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；SEE SELMER ET AL., SCIENCE 2006, pH 7.1
Resolution 3.45 Å R-free 0.257
4V5E Insights into translational termination from the structure of RF2 bound to the ribosome Deposited 2009-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 546 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；SEE SELMER ET AL., SCIENCE 2006, pH 7.1
Resolution 3.45 Å R-free 0.257
4V5F The structure of the ribosome with elongation factor G trapped in the post-translocational state Deposited 2009-09-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 62-meric
Chain B7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；100 MM MES PH 6.5, 12%PEG 20K, 50 MM KCL, 10 MM NH4CL, 3UM DEOXY-BIG-CHAP
Resolution 3.60 Å R-free 0.260
4V5F The structure of the ribosome with elongation factor G trapped in the post-translocational state Deposited 2009-09-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 62-meric
Chain D7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；100 MM MES PH 6.5, 12%PEG 20K, 50 MM KCL, 10 MM NH4CL, 3UM DEOXY-BIG-CHAP
Resolution 3.60 Å R-free 0.260
4V5G The crystal structure of the 70S ribosome bound to EF-Tu and tRNA Deposited 2009-09-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 22-25 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.4% (W/V) PEG20K
Resolution 3.60 Å R-free 0.315
4V5G The crystal structure of the 70S ribosome bound to EF-Tu and tRNA Deposited 2009-09-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 MG MAGNESIUM ION × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 22-25 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.4% (W/V) PEG20K
Resolution 3.60 Å R-free 0.315
4V5J Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release Deposited 2010-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 548 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；SEE SELMER ET AL., SCIENCE 2006, pH 6.5
Resolution 3.10 Å R-free 0.264
4V5J Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release Deposited 2010-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 546 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；SEE SELMER ET AL., SCIENCE 2006, pH 6.5
Resolution 3.10 Å R-free 0.264
4V5K Structure of cytotoxic domain of colicin E3 bound to the 70S ribosome Deposited 2010-05-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1164 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.18；AS STATED IN STRUCTURE OF THE 70S RIBOSOME COMPLEXED WITH MRNA AND TRNA. (2006) SCIENCE 313: 1935, PH 7.18
Resolution 3.20 Å R-free 0.270
4V5K Structure of cytotoxic domain of colicin E3 bound to the 70S ribosome Deposited 2010-05-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1170 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.18；AS STATED IN STRUCTURE OF THE 70S RIBOSOME COMPLEXED WITH MRNA AND TRNA. (2006) SCIENCE 313: 1935, PH 7.18
Resolution 3.20 Å R-free 0.270
4V5L The structure of EF-Tu and aminoacyl-tRNA bound to the 70S ribosome with a GTP analog Deposited 2010-09-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, AND 5.3% (W/V) PEG20K
Resolution 3.10 Å R-free 0.268
4V5M tRNA tranlocation on the 70S ribosome: the pre-translocational translocation intermediate TI(PRE) Deposited 2010-10-01 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain B7 1–49（49 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- VITROBOT (FEI)
Resolution 7.80 Å
4V5N tRNA translocation on the 70S ribosome: the post- translocational translocation intermediate TI(POST) Deposited 2010-10-21 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain B7 1–49（49 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, INSTRUMENT- VITROBOT (FEI)
Resolution 7.60 Å
4V5P The crystal structure of EF-Tu and A9C-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
Resolution 3.10 Å R-free 0.267
4V5P The crystal structure of EF-Tu and A9C-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
Resolution 3.10 Å R-free 0.267
4V5Q The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
Resolution 3.10 Å R-free 0.275
4V5Q The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a near- cognate codon on the 70S ribosome Deposited 2010-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
Resolution 3.10 Å R-free 0.275
4V5R The crystal structure of EF-Tu and Trp-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
Resolution 3.10 Å R-free 0.264
4V5R The crystal structure of EF-Tu and Trp-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
Resolution 3.10 Å R-free 0.264
4V5S The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
Resolution 3.10 Å R-free 0.285
4V5S The crystal structure of EF-Tu and G24A-tRNA-Trp bound to a cognate codon on the 70S ribosome. Deposited 2010-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 GDP GUANOSINE-5'-DIPHOSPHATE × 1 KIR KIRROMYCIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, 5.2% (W/V) PEG20K
Resolution 3.10 Å R-free 0.285
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V68 T. thermophilus 70S ribosome in complex with mRNA, tRNAs and EF-Tu.GDP.kirromycin ternary complex, fitted to a 6.4 A Cryo-EM map. Deposited 2008-12-11 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 60-meric
Chain B7 1–49（49 aa）
Not recorded PHA PHENYLALANINAL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MAU N-METHYL KIRROMYCIN × 1 BME BETA-MERCAPTOETHANOL × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen METHANE；A Vitrobot was used to shock-freeze the sample in liquid methane.
Resolution 6.40 Å
4V6A Structure of EF-P bound to the 70S ribosome. Deposited 2009-06-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 611 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5 % Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
Resolution 3.10 Å R-free 0.302
4V6A Structure of EF-P bound to the 70S ribosome. Deposited 2009-06-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 439 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5 % Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
Resolution 3.10 Å R-free 0.302
4V6F Elongation complex of the 70S ribosome with three tRNAs and mRNA. Deposited 2009-07-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 48 PDB declaration: 55-meric
Chain A7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 2089 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, temperature 293K
Resolution 3.10 Å R-free 0.255
4V6F Elongation complex of the 70S ribosome with three tRNAs and mRNA. Deposited 2009-07-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 3256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, temperature 293K
Resolution 3.10 Å R-free 0.255
4V6G Initiation complex of 70S ribosome with two tRNAs and mRNA. Deposited 2009-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 48 PDB declaration: 54-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1216 ZN ZINC ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, temperature 293K
Resolution 3.50 Å R-free 0.252
4V6G Initiation complex of 70S ribosome with two tRNAs and mRNA. Deposited 2009-07-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 48 PDB declaration: 55-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1437 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, temperature 293K
Resolution 3.50 Å R-free 0.252
4V7J Structure of RelE nuclease bound to the 70S ribosome (precleavage state) Deposited 2009-11-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric
Chain A7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 532 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；0.1M Tris-HAc, 0.2M KSCN, 3-4.5% w/v PEG 20k, 3-4.5% PEG 550 monomethylether, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.30 Å R-free 0.247
4V7J Structure of RelE nuclease bound to the 70S ribosome (precleavage state) Deposited 2009-11-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric
Chain B7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 532 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.2；293 K；0.1M Tris-HAc, 0.2M KSCN, 3-4.5% w/v PEG 20k, 3-4.5% PEG 550 monomethylether, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.30 Å R-free 0.247
4V7K Structure of RelE nuclease bound to the 70S ribosome (postcleavage state) Deposited 2009-11-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain A7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 532 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 M Tris-HAc, 0.2 M KSCN, 3-4.5% PEG20K (W/V), 3-4.5% PEG550 MME, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.60 Å R-free 0.245
4V7K Structure of RelE nuclease bound to the 70S ribosome (postcleavage state) Deposited 2009-11-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain B7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 MG MAGNESIUM ION × 532 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.2；293 K；0.1 M Tris-HAc, 0.2 M KSCN, 3-4.5% PEG20K (W/V), 3-4.5% PEG550 MME, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.60 Å R-free 0.245
4V7L The structures of viomycin bound to the 70S ribosome. Deposited 2009-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 577 ZN ZINC ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
Resolution 3.00 Å R-free 0.272
4V7L The structures of viomycin bound to the 70S ribosome. Deposited 2009-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 388 ZN ZINC ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
Resolution 3.00 Å R-free 0.272
4V7M The structures of Capreomycin bound to the 70S ribosome. Deposited 2009-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 483 ZN ZINC ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
Resolution 3.45 Å R-free 0.268
4V7M The structures of Capreomycin bound to the 70S ribosome. Deposited 2009-11-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 359 ZN ZINC ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K
Resolution 3.45 Å R-free 0.268
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B4 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain C4 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7W Structure of the Thermus thermophilus ribosome complexed with chloramphenicol. Deposited 2010-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 445 ZN ZINC ION × 2 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.00 Å R-free 0.281
4V7W Structure of the Thermus thermophilus ribosome complexed with chloramphenicol. Deposited 2010-08-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 400 ZN ZINC ION × 2 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.00 Å R-free 0.281
4V7X Structure of the Thermus thermophilus ribosome complexed with erythromycin. Deposited 2010-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 438 ZN ZINC ION × 2 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.00 Å R-free 0.278
4V7X Structure of the Thermus thermophilus ribosome complexed with erythromycin. Deposited 2010-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 392 ZN ZINC ION × 2 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.00 Å R-free 0.278
4V7Y Structure of the Thermus thermophilus 70S ribosome complexed with azithromycin. Deposited 2010-08-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 421 ZN ZINC ION × 2 K POTASSIUM ION × 1 ZIT AZITHROMYCIN × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.00 Å R-free 0.269
4V7Y Structure of the Thermus thermophilus 70S ribosome complexed with azithromycin. Deposited 2010-08-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 373 ZN ZINC ION × 2 K POTASSIUM ION × 1 ZIT AZITHROMYCIN × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.00 Å R-free 0.269
4V7Z Structure of the Thermus thermophilus 70S ribosome complexed with telithromycin. Deposited 2010-08-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 435 ZN ZINC ION × 2 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.10 Å R-free 0.284
4V7Z Structure of the Thermus thermophilus 70S ribosome complexed with telithromycin. Deposited 2010-08-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 384 ZN ZINC ION × 2 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.10 Å R-free 0.284
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain B4 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain D4 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain B4 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain D4 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V87 Crystal structure analysis of ribosomal decoding. Deposited 2011-09-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain A7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 937 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.10 Å R-free 0.240
4V87 Crystal structure analysis of ribosomal decoding. Deposited 2011-09-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 779 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.10 Å R-free 0.240
4V8A The structure of thermorubin in complex with the 70S ribosome from Thermus thermophilus. Deposited 2011-12-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain A7 1–49（49 aa）
Not recorded T8B Thermorubin × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；2.9% PEG 20K, 9% MPD, 0.1M Tris-HCl pH 7.6, 175 mM L-Arginine, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.20 Å R-free 0.273
4V8A The structure of thermorubin in complex with the 70S ribosome from Thermus thermophilus. Deposited 2011-12-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 51-meric
Chain B7 1–49（49 aa）
Not recorded T8B Thermorubin × 1 MG MAGNESIUM ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；2.9% PEG 20K, 9% MPD, 0.1M Tris-HCl pH 7.6, 175 mM L-Arginine, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.20 Å R-free 0.273
4V8B Crystal structure analysis of ribosomal decoding (near-cognate tRNA-leu complex). Deposited 2011-12-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 924 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.00 Å R-free 0.272
4V8B Crystal structure analysis of ribosomal decoding (near-cognate tRNA-leu complex). Deposited 2011-12-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 772 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.00 Å R-free 0.272
4V8C Crystal structure analysis of ribosomal decoding (near-cognate tRNA-leu complex with paromomycin). Deposited 2011-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain A7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 926 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.30 Å R-free 0.237
4V8C Crystal structure analysis of ribosomal decoding (near-cognate tRNA-leu complex with paromomycin). Deposited 2011-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 771 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.30 Å R-free 0.237
4V8D Structure analysis of ribosomal decoding (cognate tRNA-tyr complex). Deposited 2011-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 847 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.00 Å R-free 0.235
4V8D Structure analysis of ribosomal decoding (cognate tRNA-tyr complex). Deposited 2011-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 757 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.00 Å R-free 0.235
4V8E Crystal structure analysis of ribosomal decoding (near-cognate tRNA-tyr complex). Deposited 2011-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain A7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 479 OHX osmium (III) hexammine × 370 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.30 Å R-free 0.259
4V8E Crystal structure analysis of ribosomal decoding (near-cognate tRNA-tyr complex). Deposited 2011-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain C7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 415 OHX osmium (III) hexammine × 342 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.30 Å R-free 0.259
4V8F Crystal structure analysis of ribosomal decoding (near-cognate tRNA-ttyr complex with paromomycin). Deposited 2011-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain A7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 486 OHX osmium (III) hexammine × 369 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.30 Å R-free 0.247
4V8F Crystal structure analysis of ribosomal decoding (near-cognate tRNA-ttyr complex with paromomycin). Deposited 2011-12-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 417 OHX osmium (III) hexammine × 341 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.30 Å R-free 0.247
4V8G Crystal structure of RMF bound to the 70S ribosome. Deposited 2011-12-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 776 ZN ZINC ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 3.00 Å R-free 0.254
4V8G Crystal structure of RMF bound to the 70S ribosome. Deposited 2011-12-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 512 ZN ZINC ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 3.00 Å R-free 0.254
4V8H Crystal structure of HPF bound to the 70S ribosome. Deposited 2011-12-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 857 ZN ZINC ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 3.10 Å R-free 0.258
4V8H Crystal structure of HPF bound to the 70S ribosome. Deposited 2011-12-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 791 ZN ZINC ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 3.10 Å R-free 0.258
4V8I Crystal structure of YfiA bound to the 70S ribosome. Deposited 2011-12-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1042 ZN ZINC ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.70 Å R-free 0.254
4V8I Crystal structure of YfiA bound to the 70S ribosome. Deposited 2011-12-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 889 ZN ZINC ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.70 Å R-free 0.254
4V8J Crystal structure of the bacterial ribosome ram mutation G347U. Deposited 2011-12-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 369 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10MM MGCL2, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
Resolution 3.90 Å R-free 0.269
4V8J Crystal structure of the bacterial ribosome ram mutation G347U. Deposited 2011-12-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 371 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10MM MGCL2, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K
Resolution 3.90 Å R-free 0.269
4V8N The crystal structure of agmatidine tRNA-Ile2 bound to the 70S ribosome in the A and P site. Deposited 2013-02-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain B7 1–49（49 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；0.1 M TRIS-ACETATE PH 7, 0.2 M KSCN, 3.5-5.5% (W/V) PEG 20K, AND 3.5-5.5% (W/V) PEG 550 MONOMETHYL ETHER
Resolution 3.10 Å R-free 0.280
4V8N The crystal structure of agmatidine tRNA-Ile2 bound to the 70S ribosome in the A and P site. Deposited 2013-02-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain D7 1–49（49 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；0.1 M TRIS-ACETATE PH 7, 0.2 M KSCN, 3.5-5.5% (W/V) PEG 20K, AND 3.5-5.5% (W/V) PEG 550 MONOMETHYL ETHER
Resolution 3.10 Å R-free 0.280
4V8O Crystal structure of the hybrid state of ribosome in complex with the guanosine triphosphatase release factor 3 Deposited 2011-07-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain B7 1–49（49 aa）
Not recorded GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.7；100 MM MES-KOH PH 6.7, 20 MM KCL, 7.4% (W/V) PEG20K
Resolution 3.80 Å R-free 0.351
4V8Q Complex of SmpB, a tmRNA fragment and EF-Tu-GDP-Kirromycin with the 70S ribosome Deposited 2011-12-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric
Chain A7 1–49（49 aa）
Not recorded ZN ZINC ION × 3 MG MAGNESIUM ION × 2 KIR KIRROMYCIN × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；100 MM MES PH 6.5, 20 MM KCL, 8% (W/V) PEG20K
Resolution 3.10 Å R-free 0.270
4V8U Crystal Structure of 70S Ribosome with Both Cognate tRNAs in the E and P Sites Representing an Authentic Elongation Complex. Deposited 2012-08-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain B7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.4；0.1M MES, PH6.5, 8.5-9.0% PEG20K, 0-25MM KCL, pH 6.4
Resolution 3.70 Å R-free 0.249
4V8U Crystal Structure of 70S Ribosome with Both Cognate tRNAs in the E and P Sites Representing an Authentic Elongation Complex. Deposited 2012-08-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain D7 1–49（49 aa）
Not recorded ZN ZINC ION × 4 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.4；0.1M MES, PH6.5, 8.5-9.0% PEG20K, 0-25MM KCL, pH 6.4
Resolution 3.70 Å R-free 0.249
4V8X Structure of Thermus thermophilus ribosome Deposited 2013-07-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 352 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；0.1 M TRIS-HAC PH 7.2, 0.2 M KSCN, 4.1%-4.3% (W/V) PEG 20K AND 4.1%-4.3% (W/V) PEG 550MME
Resolution 3.35 Å R-free 0.261
4V8X Structure of Thermus thermophilus ribosome Deposited 2013-07-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 355 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；0.1 M TRIS-HAC PH 7.2, 0.2 M KSCN, 4.1%-4.3% (W/V) PEG 20K AND 4.1%-4.3% (W/V) PEG 550MME
Resolution 3.35 Å R-free 0.261
4V90 Thermus thermophilus Ribosome Deposited 2014-02-22 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric
Chain B7 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 523 ZN ZINC ION × 3 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；0.1M MES PH 6.5, 5.8-6.8%PEG20K, 30-120 MM KCL AND 0.8-2.5% GLYCEROL
Resolution 2.95 Å R-free 0.244
4V95 Crystal structure of YAEJ bound to the 70S ribosome Deposited 2012-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1359 ZN ZINC ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, vapor diffusion, temperature 292K
Resolution 3.20 Å R-free 0.245
4V95 Crystal structure of YAEJ bound to the 70S ribosome Deposited 2012-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 977 ZN ZINC ION × 7 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, vapor diffusion, temperature 292K
Resolution 3.20 Å R-free 0.245
4V97 Crystal structure of the bacterial ribosome ram mutation G299A. Deposited 2012-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 457 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.52 Å R-free 0.249
4V97 Crystal structure of the bacterial ribosome ram mutation G299A. Deposited 2012-04-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 576 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Resolution 3.52 Å R-free 0.249
4V9A Crystal Structure of the 70S ribosome with tetracycline. Deposited 2012-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 921 TAC TETRACYCLINE × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.30 Å R-free 0.254
4V9A Crystal Structure of the 70S ribosome with tetracycline. Deposited 2012-07-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 766 TAC TETRACYCLINE × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.30 Å R-free 0.254
4V9B Crystal Structure of the 70S ribosome with tigecycline. Deposited 2012-07-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 922 T1C TIGECYCLINE × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.10 Å R-free 0.269
4V9B Crystal Structure of the 70S ribosome with tigecycline. Deposited 2012-07-18 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 764 T1C TIGECYCLINE × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K
Resolution 3.10 Å R-free 0.269
4V9H Crystal structure of the ribosome bound to elongation factor G in the guanosine triphosphatase state Deposited 2013-03-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 58-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 138 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.3；294 K；100mM MES pH 6.3, 75mM KCl, 6.0% PEG 20K, VAPOR DIFFUSION, HANGING DROP, temperature 294K
Resolution 2.86 Å R-free 0.250
4V9I Crystal structure of thermus thermophilus 70S in complex with tRNAs and mRNA containing a pseudouridine in a stop codon Deposited 2013-04-04 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain B7 1–48（48 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.30 Å R-free 0.279
4V9I Crystal structure of thermus thermophilus 70S in complex with tRNAs and mRNA containing a pseudouridine in a stop codon Deposited 2013-04-04 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain D7 1–48（48 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.30 Å R-free 0.279
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B7 1–49（49 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D7 1–49（49 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B7 1–49（49 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D7 1–49（49 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B7 1–49（49 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D7 1–49（49 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain B7 1–49（49 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain D7 1–49（49 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B7 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain D7 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain A4 1–48（48 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain C4 1–48（48 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9R Crystal structure of antibiotic DITYROMYCIN bound to 70S ribosome Deposited 2013-12-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1086 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 3.00 Å R-free 0.259
4V9R Crystal structure of antibiotic DITYROMYCIN bound to 70S ribosome Deposited 2013-12-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 887 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 3.00 Å R-free 0.259
4V9S Crystal structure of antibiotic GE82832 bound to 70S ribosome Deposited 2013-12-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1086 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 3.10 Å R-free 0.260
4V9S Crystal structure of antibiotic GE82832 bound to 70S ribosome Deposited 2013-12-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 887 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 FME N-FORMYLMETHIONINE × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 3.10 Å R-free 0.260
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain B7 1–49（49 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain D7 1–49（49 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W2E Crystal structure of Elongation Factor 4 (EF4/LepA) bound to the Thermus thermophilus 70S ribosome Deposited 2014-06-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 51 PDB declaration: 57-meric
Chain 7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 906 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.90 Å R-free 0.304
4W2F Crystal structure of the Thermus thermophilus 70S ribosome in complex with amicoumacin, mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1338 UAM Amicoumacin A × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.40 Å R-free 0.277
4W2F Crystal structure of the Thermus thermophilus 70S ribosome in complex with amicoumacin, mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 969 UAM Amicoumacin A × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.40 Å R-free 0.277
4W2G Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (soaked), mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1218 PCY Pactamycin × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.55 Å R-free 0.273
4W2G Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (soaked), mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 916 PCY Pactamycin × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.55 Å R-free 0.273
4W2H Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (co-crystallized), mRNA and deacylated tRNA in the P site Deposited 2014-09-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1135 PCY Pactamycin × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.70 Å R-free 0.262
4W2H Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (co-crystallized), mRNA and deacylated tRNA in the P site Deposited 2014-09-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 908 PCY Pactamycin × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.70 Å R-free 0.262
4W2I Crystal structure of the Thermus thermophilus 70S ribosome in complex with negamycin, mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1147 NEG NEGAMYCIN × 9 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.70 Å R-free 0.257
4W2I Crystal structure of the Thermus thermophilus 70S ribosome in complex with negamycin, mRNA and three deacylated tRNAs in the A, P and E sites Deposited 2014-09-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 866 NEG NEGAMYCIN × 10 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K
Resolution 2.70 Å R-free 0.257
4W4G Postcleavage state of 70S bound to HigB toxin and AAA (lysine) codon Deposited 2014-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 581 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
Resolution 3.30 Å R-free 0.238
4W4G Postcleavage state of 70S bound to HigB toxin and AAA (lysine) codon Deposited 2014-08-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 659 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
Resolution 3.30 Å R-free 0.238
4WPO Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the pre-translocational state Deposited 2014-10-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric
Chain A7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1163 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
Resolution 2.80 Å R-free 0.252
4WPO Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the pre-translocational state Deposited 2014-10-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric
Chain C7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 893 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
Resolution 2.80 Å R-free 0.252
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQF Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G and fusidic acid in the post-translocational state Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric
Chain A7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1147 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
Resolution 2.80 Å R-free 0.255
4WQF Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G and fusidic acid in the post-translocational state Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric
Chain C7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 893 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
Resolution 2.80 Å R-free 0.255
4WQR Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 726 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.15 Å R-free 0.231
4WQR Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.15 Å R-free 0.231
4WQU Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G trapped by the antibiotic dityromycin Deposited 2014-10-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric
Chain A7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1147 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
Resolution 2.80 Å R-free 0.264
4WQU Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G trapped by the antibiotic dityromycin Deposited 2014-10-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 53 PDB declaration: 59-meric
Chain C7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 893 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
Resolution 2.80 Å R-free 0.264
4WQY Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the post-translocational state (without fusitic acid) Deposited 2014-10-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 57-meric
Chain A7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1155 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
Resolution 2.80 Å R-free 0.266
4WQY Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the post-translocational state (without fusitic acid) Deposited 2014-10-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 57-meric
Chain C7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 890 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
Resolution 2.80 Å R-free 0.266
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRO Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site Deposited 2014-10-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 727 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.231
4WSD Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site and with antibiotic paromomycin. Deposited 2014-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 713 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 2.95 Å R-free 0.235
4WSD Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site and with antibiotic paromomycin. Deposited 2014-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 525 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 2.95 Å R-free 0.235
4WSM Complex of 70S ribosome with tRNA-Leu and mRNA with G-U mismatch in the first position in the A- and P-sites Deposited 2014-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 685 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.30 Å
4WSM Complex of 70S ribosome with tRNA-Leu and mRNA with G-U mismatch in the first position in the A- and P-sites Deposited 2014-10-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 649 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.30 Å
4WT1 Complex of 70S ribosome with tRNA-Phe and mRNA with A-A mismatch in the second position in the A-site Deposited 2014-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 701 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å
4WT1 Complex of 70S ribosome with tRNA-Phe and mRNA with A-A mismatch in the second position in the A-site Deposited 2014-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 546 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å
4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 51 PDB declaration: 61-meric
Chain C8 1–48（48 aa）
Not recorded PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME
Resolution 3.40 Å R-free 0.241
4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 51 PDB declaration: 62-meric
Chain D8 1–48（48 aa）
Not recorded PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME
Resolution 3.40 Å R-free 0.241
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZO Complex of 70S ribosome with tRNA-fMet and mRNA Deposited 2014-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 56-MERIC
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 576 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.30 Å R-free 0.258
4WZO Complex of 70S ribosome with tRNA-fMet and mRNA Deposited 2014-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-MERIC
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 413 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.30 Å R-free 0.258
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL34 1–48（48 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL34 1–48（48 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4Y4O Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to protein Y (YfiA) at 2.3A resolution Deposited 2015-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1495 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.30 Å R-free 0.247
4Y4O Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to protein Y (YfiA) at 2.3A resolution Deposited 2015-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1022 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.30 Å R-free 0.247
4Y4P Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to mRNA and A-, P- and E-site tRNAs at 2.5A resolution Deposited 2015-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1572 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.281
4Y4P Crystal structure of the Thermus thermophilus 70S ribosome with rRNA modifications and bound to mRNA and A-, P- and E-site tRNAs at 2.5A resolution Deposited 2015-02-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1265 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.281
4YPB Precleavage 70S structure of the P. vulgaris HigB DeltaH92 toxin bound to the AAA codon Deposited 2015-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 624 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
Resolution 3.40 Å R-free 0.234
4YPB Precleavage 70S structure of the P. vulgaris HigB DeltaH92 toxin bound to the AAA codon Deposited 2015-03-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 684 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
Resolution 3.40 Å R-free 0.234
4YZV Precleavage 70S structure of the P. vulgaris HigB deltaH92 toxin bound to the ACA codon Deposited 2015-03-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 638 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
Resolution 3.10 Å R-free 0.237
4YZV Precleavage 70S structure of the P. vulgaris HigB deltaH92 toxin bound to the ACA codon Deposited 2015-03-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
Resolution 3.10 Å R-free 0.237
4Z3S Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic A201A, mRNA and three tRNAs in the A, P and E sites at 2.65A resolution Deposited 2015-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1328 4M2 3'-deoxy-3'-{[(2E)-3-(4-{[(4Z)-6-O-(6-deoxy-3,4-di-O-methyl-alpha-D-mannopyranosyl)-5-O-methyl-alpha-D-threo-hex-4-enofuranosyl]oxy}phenyl)-2-methylprop-2-enoyl]amino}-N,N-dimethyladenosine × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.65 Å R-free 0.271
4Z3S Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic A201A, mRNA and three tRNAs in the A, P and E sites at 2.65A resolution Deposited 2015-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 964 4M2 3'-deoxy-3'-{[(2E)-3-(4-{[(4Z)-6-O-(6-deoxy-3,4-di-O-methyl-alpha-D-mannopyranosyl)-5-O-methyl-alpha-D-threo-hex-4-enofuranosyl]oxy}phenyl)-2-methylprop-2-enoyl]amino}-N,N-dimethyladenosine × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.65 Å R-free 0.271
4Z8C Crystal structure of the Thermus thermophilus 70S ribosome bound to translation inhibitor oncocin Deposited 2015-04-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1448 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.90 Å R-free 0.248
4Z8C Crystal structure of the Thermus thermophilus 70S ribosome bound to translation inhibitor oncocin Deposited 2015-04-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 890 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.90 Å R-free 0.248
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 17 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 27 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZSN 70S-wild-type HigB toxin complex bound to a AAA lysine codon Deposited 2015-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 610 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
Resolution 3.60 Å R-free 0.234
4ZSN 70S-wild-type HigB toxin complex bound to a AAA lysine codon Deposited 2015-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 671 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN
Resolution 3.60 Å R-free 0.234
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain Ad 1–49（49 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain Ad 1–49（49 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5CZP 70S termination complex containing E. coli RF2 Deposited 2015-07-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1027 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.9% PEG 20k, 9.8% PEG 200, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MAGNESIUM ACETATE
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.4% PEG 20k, 10.8% PEG 200, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MAGNESIUM ACETATE
Resolution 3.30 Å R-free 0.286
5CZP 70S termination complex containing E. coli RF2 Deposited 2015-07-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1493 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.9% PEG 20k, 9.8% PEG 200, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MAGNESIUM ACETATE
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.4% PEG 20k, 10.8% PEG 200, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MAGNESIUM ACETATE
Resolution 3.30 Å R-free 0.286
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain AA 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain WB 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5DFE 70S termination complex containing E. coli RF2 Deposited 2015-08-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.5% PEG 20k, 11% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.8% PEG 20k, 11.6% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.10 Å R-free 0.227
5DFE 70S termination complex containing E. coli RF2 Deposited 2015-08-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1025 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.5% PEG 20k, 11% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.8% PEG 20k, 11.6% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.10 Å R-free 0.227
5DOX Crystal structure of the Thermus thermophilus 70S ribosome in complex with Hygromycin-A at 3.1A resolution Deposited 2015-09-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1019 HGR Hygromycin A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.10 Å R-free 0.284
5DOX Crystal structure of the Thermus thermophilus 70S ribosome in complex with Hygromycin-A at 3.1A resolution Deposited 2015-09-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 699 HGR Hygromycin A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.10 Å R-free 0.284
5DOY Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic Hygromycin A, mRNA and three tRNAs in the A, P and E sites at 2.6A resolution Deposited 2015-09-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1358 K POTASSIUM ION × 1 HGR Hygromycin A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.283
5DOY Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic Hygromycin A, mRNA and three tRNAs in the A, P and E sites at 2.6A resolution Deposited 2015-09-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 943 K POTASSIUM ION × 1 HGR Hygromycin A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.283
5E7K Structure of T. thermophilus 70S ribosome complex with mRNA and cognate tRNALys in the A-site Deposited 2015-10-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 628 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.20 Å R-free 0.258
5E7K Structure of T. thermophilus 70S ribosome complex with mRNA and cognate tRNALys in the A-site Deposited 2015-10-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 510 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.20 Å R-free 0.258
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5EL4 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 671 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.15 Å R-free 0.251
5EL4 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 536 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.15 Å R-free 0.251
5EL5 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-MERIC
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 660 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.15 Å R-free 0.254
5EL5 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-MERIC
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 522 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.15 Å R-free 0.254
5EL6 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position and antibiotic paromomycin Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 593 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.10 Å R-free 0.249
5EL6 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the first position and antibiotic paromomycin Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 480 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.10 Å R-free 0.249
5EL7 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position and antibiotic paromomycin Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 687 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.15 Å R-free 0.247
5EL7 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with a U-U mismatch in the second position and antibiotic paromomycin Deposited 2015-11-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 574 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.15 Å R-free 0.247
5F8K Crystal structure of the Bac7(1-16) antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 17 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1363 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 2.80 Å R-free 0.292
5F8K Crystal structure of the Bac7(1-16) antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 27 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1340 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 2.80 Å R-free 0.292
5FDU Crystal structure of the Metalnikowin I antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric
Chain 17 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1292 AMP ADENOSINE MONOPHOSPHATE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 2.90 Å R-free 0.234
5FDU Crystal structure of the Metalnikowin I antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric
Chain 27 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1127 AMP ADENOSINE MONOPHOSPHATE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 2.90 Å R-free 0.234
5FDV Crystal structure of the Pyrrhocoricin antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric
Chain 17 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1292 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 2.80 Å R-free 0.240
5FDV Crystal structure of the Pyrrhocoricin antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric
Chain 27 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1128 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 2.80 Å R-free 0.240
5HAU Crystal structure of antimicrobial peptide Bac7(1-19) bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 53 PDB declaration: 58-meric
Chain 15 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1081 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 3.00 Å R-free 0.265
5HAU Crystal structure of antimicrobial peptide Bac7(1-19) bound to the Thermus thermophilus 70S ribosome Deposited 2015-12-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 53 PDB declaration: 58-meric
Chain 25 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 848 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 3.00 Å R-free 0.265
5HCP Crystal structure of antimicrobial peptide Metalnikowin bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1593 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 2.89 Å R-free 0.268
5HCP Crystal structure of antimicrobial peptide Metalnikowin bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 887 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 2.89 Å R-free 0.268
5HCQ Crystal structure of antimicrobial peptide Oncocin d15-19 bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1540 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.251
5HCQ Crystal structure of antimicrobial peptide Oncocin d15-19 bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 890 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.251
5HCR Crystal structure of antimicrobial peptide Oncocin 10wt bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1585 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.268
5HCR Crystal structure of antimicrobial peptide Oncocin 10wt bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 837 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.268
5HD1 Crystal structure of antimicrobial peptide Pyrrhocoricin bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1701 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.272
5HD1 Crystal structure of antimicrobial peptide Pyrrhocoricin bound to the Thermus thermophilus 70S ribosome Deposited 2016-01-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 900 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；292 K；0.15M Arginine-HCl, 0.1M Tris-HCl pH 7.3-7.6, 2.6-2.9% PEG-20K, 9-10% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.272
5IB7 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet, near-cognate tRNALys with U-G mismatch in the A-site and antibiotic paromomycin Deposited 2016-02-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 695 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.99 Å R-free 0.241
5IB7 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet, near-cognate tRNALys with U-G mismatch in the A-site and antibiotic paromomycin Deposited 2016-02-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 563 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 SPE THERMINE × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.99 Å R-free 0.241
5IB8 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNALys with U-G mismatch in the A-site Deposited 2016-02-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 723 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.13 Å R-free 0.244
5IB8 Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNALys with U-G mismatch in the A-site Deposited 2016-02-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 614 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 SPE THERMINE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 3.13 Å R-free 0.244
5IBB Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAVal in the A-site Deposited 2016-02-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 736 SPE THERMINE × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.96 Å R-free 0.243
5IBB Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAVal in the A-site Deposited 2016-02-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 608 SPE THERMINE × 4 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.96 Å R-free 0.243
5IMQ Structure of ribosome bound to cofactor at 3.8 angstrom resolution Deposited 2016-03-06 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain y 1–49（49 aa）
Not recorded GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Buffer G 5 mM HEPES pH 7.5, 10 mM MgAc, 50 mM KCl, 10 mM NH4Cl, and 6 mM 2-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5IMR Structure of ribosome bound to cofactor at 5.7 angstrom resolution Deposited 2016-03-06 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain y 1–49（49 aa）
Not recorded GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5；Buffer G 5 mM HEPES pH 7.5, 10 mM MgAc, 50 mM KCl, 10 mM NH4Cl, and 6 mM 2-mercaptoethanol
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.70 Å
5J30 Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1431 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.1% PEG 20k, 10.2% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.20 Å R-free 0.244
5J30 Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1000 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.1% PEG 20k, 10.2% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.20 Å R-free 0.244
5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1425 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.04 Å R-free 0.279
5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1011 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.04 Å R-free 0.279
5J4B Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (co-crystallized) and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1706 K POTASSIUM ION × 1 CPT Cisplatin × 9 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.264
5J4B Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (co-crystallized) and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1311 K POTASSIUM ION × 1 CPT Cisplatin × 7 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.264
5J4C Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (soaked) and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1796 CPT Cisplatin × 4 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.268
5J4C Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (soaked) and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1275 CPT Cisplatin × 4 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.268
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain EA 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain JC 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J8B Crystal structure of Elongation Factor 4 (EF-4/LepA) in complex with GDPCP bound to the Thermus thermophilus 70S ribosome Deposited 2016-04-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 59-meric
Chain 7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 919 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME
Resolution 2.60 Å R-free 0.261
5NDJ Crystal structure of aminoglycoside TC007 in complex with 70S ribosome from Thermus thermophilus, three tRNAs and mRNA (soaking) Deposited 2017-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain P8 1–49（49 aa）
Not recorded 8UZ TC007 × 6 MG MAGNESIUM ION × 931 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN
Resolution 3.15 Å R-free 0.247
5NDJ Crystal structure of aminoglycoside TC007 in complex with 70S ribosome from Thermus thermophilus, three tRNAs and mRNA (soaking) Deposited 2017-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain L5 1–49（49 aa）
Not recorded 8UZ TC007 × 7 MG MAGNESIUM ION × 839 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN
Resolution 3.15 Å R-free 0.247
5NDK Crystal structure of aminoglycoside TC007 co-crystallized with 70S ribosome from Thermus thermophilus, three tRNAs and mRNA Deposited 2017-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain P8 1–49（49 aa）
Not recorded 8UZ TC007 × 2 MG MAGNESIUM ION × 846 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN
Resolution 2.95 Å R-free 0.253
5NDK Crystal structure of aminoglycoside TC007 co-crystallized with 70S ribosome from Thermus thermophilus, three tRNAs and mRNA Deposited 2017-03-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain L5 1–49（49 aa）
Not recorded 8UZ TC007 × 2 MG MAGNESIUM ION × 800 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN
Resolution 2.95 Å R-free 0.253
5OT7 Elongation factor G-ribosome complex captures in the absence of inhibitors. Deposited 2017-08-21 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 59-meric
Chain c 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 462 ZN ZINC ION × 3 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.80 Å
5UQ7 70S ribosome complex with dnaX mRNA stemloop and E-site tRNA ("in" conformation) Deposited 2017-02-07 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain 7 1–48（48 aa）
Not recorded ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.50 Å
5UQ8 70S ribosome complex with dnaX mRNA stem-loop and E-site tRNA ("out" conformation) Deposited 2017-02-07 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain 7 1–48（48 aa）
Not recorded ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 17 1–49（49 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 27 1–49（49 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5VP2 Crystal structure of the Thermus thermophilus 70S ribosome in complex with madumycin II and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution Deposited 2017-05-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1587 K POTASSIUM ION × 1 M2D Madumycin II × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.278
5VP2 Crystal structure of the Thermus thermophilus 70S ribosome in complex with madumycin II and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution Deposited 2017-05-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1216 K POTASSIUM ION × 1 M2D Madumycin II × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.278
5VPO The 70S P-site ASL SufA6 complex Deposited 2017-05-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 325 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
Resolution 3.34 Å R-free 0.267
5VPO The 70S P-site ASL SufA6 complex Deposited 2017-05-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 352 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
Resolution 3.34 Å R-free 0.267
5VPP The 70S P-site tRNA SufA6 complex Deposited 2017-05-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 768 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
Resolution 3.90 Å
5VPP The 70S P-site tRNA SufA6 complex Deposited 2017-05-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 53-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 547 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5% PEG 20k, 5% PEG 550 MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM CHLORIDE
Resolution 3.90 Å
5W4K Crystal structure of the Thermus thermophilus 70S ribosome in complex with Klebsazolicin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution Deposited 2017-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1724 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.252
5W4K Crystal structure of the Thermus thermophilus 70S ribosome in complex with Klebsazolicin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution Deposited 2017-06-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1201 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.252
5WIS Crystal structure of the Thermus thermophilus 70S ribosome in complex with methymycin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution Deposited 2017-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1587 MT9 (3R,4S,5S,7R,9E,11S,12R)-12-ethyl-11-hydroxy-3,5,7,11-tetramethyl-2,8-dioxooxacyclododec-9-en-4-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.266
5WIS Crystal structure of the Thermus thermophilus 70S ribosome in complex with methymycin and bound to mRNA and A-, P- and E-site tRNAs at 2.7A resolution Deposited 2017-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1262 MT9 (3R,4S,5S,7R,9E,11S,12R)-12-ethyl-11-hydroxy-3,5,7,11-tetramethyl-2,8-dioxooxacyclododec-9-en-4-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.266
5WIT Crystal structure of the Thermus thermophilus 70S ribosome in complex with pikromycin and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2017-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1411 K POTASSIUM ION × 1 AQJ (3R,5R,6S,7S,9R,11E,13S,14R)-14-ethyl-13-hydroxy-3,5,7,9,13-pentamethyl-2,4,10-trioxo-1-oxacyclotetradec-11-en-6-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.283
5WIT Crystal structure of the Thermus thermophilus 70S ribosome in complex with pikromycin and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2017-07-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 982 K POTASSIUM ION × 1 AQJ (3R,5R,6S,7S,9R,11E,13S,14R)-14-ethyl-13-hydroxy-3,5,7,9,13-pentamethyl-2,4,10-trioxo-1-oxacyclotetradec-11-en-6-yl 3,4,6-trideoxy-3-(dimethylamino)-beta-D-xylo-hexopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.283
5ZLU Ribosome Structure bound to ABC-F protein. Deposited 2018-03-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 57-meric
Chain z 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 2 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.60 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain EA 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain IC 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain EA 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain JC 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BUW Thermus thermophilus 70S complex containing 16S G299A ram mutation and empty A site. Deposited 2017-12-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 719 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
Resolution 3.50 Å R-free 0.250
6BUW Thermus thermophilus 70S complex containing 16S G299A ram mutation and empty A site. Deposited 2017-12-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 731 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
Resolution 3.50 Å R-free 0.250
6BZ6 Thermus thermophilus 70S complex containing 16S G347U ram mutation and empty A site Deposited 2017-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 641 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.8% PEG 20k, 4.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.9% PEG 20k, 4.9% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
Resolution 3.18 Å R-free 0.251
6BZ6 Thermus thermophilus 70S complex containing 16S G347U ram mutation and empty A site Deposited 2017-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 703 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.8% PEG 20k, 4.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.9% PEG 20k, 4.9% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
Resolution 3.18 Å R-free 0.251
6BZ7 Thermus thermophilus 70S containing 16S G299A point mutation and near-cognate ASL Leucine in A site. Deposited 2017-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 618 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.1% PEG 20k, 4.1% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
Resolution 3.68 Å R-free 0.274
6BZ7 Thermus thermophilus 70S containing 16S G299A point mutation and near-cognate ASL Leucine in A site. Deposited 2017-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 669 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；3.8% PEG 20k, 3.8% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.1% PEG 20k, 4.1% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
Resolution 3.68 Å R-free 0.274
6BZ8 Thermus thermophilus 70S containing 16S G347U point mutation and near-cognate ASL Leucine in A site Deposited 2017-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 624 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.7% PEG 20k, 4.7% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
Resolution 3.74 Å R-free 0.225
6BZ8 Thermus thermophilus 70S containing 16S G347U point mutation and near-cognate ASL Leucine in A site Deposited 2017-12-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 668 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.7% PEG 20k, 4.7% PEG 550MME, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
Resolution 3.74 Å R-free 0.225
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CAE Crystal structure of the Thermus thermophilus 70S ribosome in complex with NOSO-95179 antibiotic and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2018-01-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1651 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.249
6CAE Crystal structure of the Thermus thermophilus 70S ribosome in complex with NOSO-95179 antibiotic and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution Deposited 2018-01-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1175 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.249
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CZR The structure of amicetin bound to the 70S ribosome Deposited 2018-04-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 17 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1163 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 FSD amicetin × 1 ZN ZINC ION × 4 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 3.14 Å R-free 0.292
6CZR The structure of amicetin bound to the 70S ribosome Deposited 2018-04-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 27 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 770 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 FSD amicetin × 1 ZN ZINC ION × 5 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 3.14 Å R-free 0.292
6FKR Crystal structure of the dolphin proline-rich antimicrobial peptide Tur1A bound to the Thermus thermophilus 70S ribosome Deposited 2018-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 53-meric
Chain 17 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1347 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl pH 7.6, PEG 20,000, 2-methyl-2,4-pentanediol, L-arginine
Resolution 3.20 Å R-free 0.252
6FKR Crystal structure of the dolphin proline-rich antimicrobial peptide Tur1A bound to the Thermus thermophilus 70S ribosome Deposited 2018-01-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–48（48 aa）
Not recorded MG MAGNESIUM ION × 1347 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl pH 7.6, PEG 20,000, 2-methyl-2,4-pentanediol, L-arginine
Resolution 3.20 Å R-free 0.252
6GSJ Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAThr in the A-site Deposited 2018-06-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 874 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
Resolution 2.96 Å R-free 0.252
6GSJ Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAThr in the A-site Deposited 2018-06-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 620 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
Resolution 2.96 Å R-free 0.252
6GSK Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNAThr in the A-site Deposited 2018-06-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 638 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
Resolution 3.36 Å R-free 0.264
6GSK Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and near-cognate tRNAThr in the A-site Deposited 2018-06-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 46 PDB declaration: 53-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 444 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
Resolution 3.36 Å R-free 0.264
6GSL Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAArg in the A-site Deposited 2018-06-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 60-meric
Chain P8 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 954 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
Resolution 3.16 Å
6GSL Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAArg in the A-site Deposited 2018-06-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-meric
Chain L5 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 810 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；Tris-OAc, KSCN, PEG-20000, PEG-550mme
Resolution 3.16 Å
6GZQ T. thermophilus hibernating 70S ribosome Deposited 2018-07-04 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain c1 1–49（49 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.28 Å
6GZX T. thermophilus hibernating 100S ribosome (ice) Deposited 2018-07-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 102 PDB declaration: 108-meric
Chain c1 1–49（49 aa）
Chain c2 1–49（49 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.57 Å
6GZZ T. thermophilus hibernating 100S ribosome (amc) Deposited 2018-07-05 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 102 PDB declaration: 108-meric
Chain c1 1–49（49 aa）
Chain c2 1–49（49 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.13 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL34 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL34 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 632 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 524 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 710 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 629 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279
6ND5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with chloramphenicol and bound to mRNA and A-, P-, and E-site tRNAs at 2.60A resolution Deposited 2018-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1576 CLM CHLORAMPHENICOL × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.276
6ND5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with chloramphenicol and bound to mRNA and A-, P-, and E-site tRNAs at 2.60A resolution Deposited 2018-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1246 CLM CHLORAMPHENICOL × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.276
6ND6 Crystal structure of the Thermus thermophilus 70S ribosome in complex with erythromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.85A resolution Deposited 2018-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1523 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.85 Å R-free 0.305
6ND6 Crystal structure of the Thermus thermophilus 70S ribosome in complex with erythromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.85A resolution Deposited 2018-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1222 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.85 Å R-free 0.305
6NDK Structure of ASLSufA6 A37.5 bound to the 70S A site Deposited 2018-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 596 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4.5-5.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.64 Å R-free 0.250
6NDK Structure of ASLSufA6 A37.5 bound to the 70S A site Deposited 2018-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 725 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4.5-5.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.64 Å R-free 0.250
6NSH Modified ASL proline bound to Thermus thermophilus 70S (near-cognate) Deposited 2019-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 575 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
Resolution 3.40 Å R-free 0.237
6NSH Modified ASL proline bound to Thermus thermophilus 70S (near-cognate) Deposited 2019-01-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 624 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
Resolution 3.40 Å R-free 0.237
6NTA Modified ASL proline bound to Thermus thermophilus 70S (cognate) Deposited 2019-01-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 592 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
Resolution 3.10 Å R-free 0.274
6NTA Modified ASL proline bound to Thermus thermophilus 70S (cognate) Deposited 2019-01-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 679 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
Resolution 3.10 Å R-free 0.274
6NUO Modified tRNA(Pro) bound to Thermus thermophilus 70S (cognate) Deposited 2019-02-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 531 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
Resolution 3.20 Å R-free 0.256
6NUO Modified tRNA(Pro) bound to Thermus thermophilus 70S (cognate) Deposited 2019-02-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 621 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
Resolution 3.20 Å R-free 0.256
6NWY Modified tRNA(Pro) bound to Thermus thermophilus 70S (near-cognate) Deposited 2019-02-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 547 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
Resolution 3.50 Å R-free 0.258
6NWY Modified tRNA(Pro) bound to Thermus thermophilus 70S (near-cognate) Deposited 2019-02-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 551 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；0.1 M Tris-HOAc, pH 7.6, 0.15 M L-arginine HCl, 2.9% (v/v) PEG 20K, 12% (v/v) MPD, 0.0005M BME
Resolution 3.50 Å R-free 0.258
6O3M Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (cognate) Deposited 2019-02-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 466 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；12% MPD 0.1M, Arginine-HCl, 0.1M Tris-HCl pH7.6, 2.9% PEG 20K, 0.0006M BME
Resolution 3.97 Å R-free 0.255
6O3M Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (cognate) Deposited 2019-02-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 542 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；12% MPD 0.1M, Arginine-HCl, 0.1M Tris-HCl pH7.6, 2.9% PEG 20K, 0.0006M BME
Resolution 3.97 Å R-free 0.255
6O97 Crystal structure of the Thermus thermophilus 70S ribosome in complex with propylamycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.75A resolution Deposited 2019-03-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1565 LUJ (1R,2R,3S,4R,6S)-4,6-diamino-2-{[3-O-(2,6-diamino-2,6-dideoxy-beta-L-idopyranosyl)-beta-D-ribofuranosyl]oxy}-3-hydroxyc yclohexyl 2-amino-2,4-dideoxy-4-propyl-alpha-D-glucopyranoside × 3 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.75 Å R-free 0.284
6O97 Crystal structure of the Thermus thermophilus 70S ribosome in complex with propylamycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.75A resolution Deposited 2019-03-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1191 LUJ (1R,2R,3S,4R,6S)-4,6-diamino-2-{[3-O-(2,6-diamino-2,6-dideoxy-beta-L-idopyranosyl)-beta-D-ribofuranosyl]oxy}-3-hydroxyc yclohexyl 2-amino-2,4-dideoxy-4-propyl-alpha-D-glucopyranoside × 3 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.75 Å R-free 0.284
6OF1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with dirithromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.80A resolution Deposited 2019-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1589 DI0 Dirithromycin × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.263
6OF1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with dirithromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.80A resolution Deposited 2019-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1194 DI0 Dirithromycin × 1 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.263
6OF6 Crystal structure of tRNA^ Ala(GGC) bound to cognate 70S A-site Deposited 2019-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 546 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 AMP ADENOSINE MONOPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.20 Å R-free 0.232
6OF6 Crystal structure of tRNA^ Ala(GGC) bound to cognate 70S A-site Deposited 2019-03-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 669 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.20 Å R-free 0.232
6OJ2 Crystal structure of tRNA^ Ala(GGC) bound to the near-cognate 70S A-site Deposited 2019-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 433 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.20 Å R-free 0.270
6OJ2 Crystal structure of tRNA^ Ala(GGC) bound to the near-cognate 70S A-site Deposited 2019-04-10 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 517 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.20 Å R-free 0.270
6OPE Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to near-cognate 70S A site Deposited 2019-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 378 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME
Resolution 3.10 Å R-free 0.266
6OPE Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to near-cognate 70S A site Deposited 2019-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 469 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME
Resolution 3.10 Å R-free 0.266
6ORD Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to cognate 70S A site Deposited 2019-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1036 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME
Resolution 3.10 Å R-free 0.268
6ORD Crystal structure of tRNA^ Ala(GGC) U32-A38 bound to cognate 70S A site Deposited 2019-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 975 PAR PAROMOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；0.1M L-arginine HCl, 0.1M Tris-HCl pH 7.5, 3% PEG 20K, 10-16.5% MPD (2-methyl-2,4-pentanediol), 1mM BME
Resolution 3.10 Å R-free 0.268
6OSI Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (near cognate) Deposited 2019-05-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 522 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 4.14 Å R-free 0.294
6OSI Unmodified tRNA(Pro) bound to Thermus thermophilus 70S (near cognate) Deposited 2019-05-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 504 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 4.14 Å R-free 0.294
6OTR Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (AAU) Deposited 2019-05-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 410 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 3 A3P ADENOSINE-3'-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME
Resolution 3.12 Å R-free 0.257
6OTR Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (AAU) Deposited 2019-05-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 464 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 A3P ADENOSINE-3'-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME
Resolution 3.12 Å R-free 0.257
6OXA Dimeric E.coli YoeB bound to Thermus thermophilus 70S pre-cleavage (AAU) Deposited 2019-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 420 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1 M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME
Resolution 3.25 Å R-free 0.256
6OXA Dimeric E.coli YoeB bound to Thermus thermophilus 70S pre-cleavage (AAU) Deposited 2019-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 58-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 476 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1 M Tris-HAc, 0.2M KSCN, PEG 5.1%, 5.1% PEG550MME
Resolution 3.25 Å R-free 0.256
6OXI Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (UAA) Deposited 2019-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 419 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1 M Tris-HAc, 0.2 M KSCN PEG 5.2%, 5.2% PEG550MME
Resolution 3.50 Å R-free 0.246
6OXI Dimeric E.coli YoeB bound to Thermus thermophilus 70S post-cleavage (UAA) Deposited 2019-05-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 471 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293.15 K；0.1 M Tris-HAc, 0.2 M KSCN PEG 5.2%, 5.2% PEG550MME
Resolution 3.50 Å R-free 0.246
6Q95 Structure of tmRNA SmpB bound in A site of T. thermophilus 70S ribosome Deposited 2018-12-17 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric
Chain d 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 637 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å
6QNQ 70S ribosome initiation complex (IC) with experimentally assigned potassium ions Deposited 2019-02-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain P8 1–49（49 aa）
Not recorded K POTASSIUM ION × 169 MG MAGNESIUM ION × 543 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20K, PEG-550mme
Resolution 3.50 Å R-free 0.251
6QNQ 70S ribosome initiation complex (IC) with experimentally assigned potassium ions Deposited 2019-02-11 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain L5 1–49（49 aa）
Not recorded K POTASSIUM ION × 127 MG MAGNESIUM ION × 426 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20K, PEG-550mme
Resolution 3.50 Å R-free 0.251
6QNR 70S ribosome elongation complex (EC) with experimentally assigned potassium ions Deposited 2019-02-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain P8 1–49（49 aa）
Not recorded K POTASSIUM ION × 211 MG MAGNESIUM ION × 585 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20k, PEG-550mme
Resolution 3.10 Å R-free 0.253
6QNR 70S ribosome elongation complex (EC) with experimentally assigned potassium ions Deposited 2019-02-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain L5 1–49（49 aa）
Not recorded K POTASSIUM ION × 155 MG MAGNESIUM ION × 409 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；Tris-OAc, KSCN, PEG-20k, PEG-550mme
Resolution 3.10 Å R-free 0.253
6UCQ Crystal structure of the Thermus thermophilus 70S ribosome recycling complex Deposited 2019-09-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 50 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1523 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.3；292 K；0.15 M L-arginine-HCl, 0.1M Tris-HCl pH 7.3, 2.4-2.7% PEG-20K, 7-9% MPD, 0.5 mM BME
Resolution 3.50 Å R-free 0.294
6UCQ Crystal structure of the Thermus thermophilus 70S ribosome recycling complex Deposited 2019-09-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 50 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1350 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.3；292 K；0.15 M L-arginine-HCl, 0.1M Tris-HCl pH 7.3, 2.4-2.7% PEG-20K, 7-9% MPD, 0.5 mM BME
Resolution 3.50 Å R-free 0.294
6UO1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA (containing pseudouridine at the first position of the codon) and deacylated A-, P-, and E-site tRNAs at 2.95A resolution Deposited 2019-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 918 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.95 Å R-free 0.292
6UO1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA (containing pseudouridine at the first position of the codon) and deacylated A-, P-, and E-site tRNAs at 2.95A resolution Deposited 2019-10-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 534 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.95 Å R-free 0.292
6XHV Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.40A resolution Deposited 2020-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1568 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.286
6XHV Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.40A resolution Deposited 2020-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1248 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.286
6XHW Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.50A resolution Deposited 2020-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1552 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.281
6XHW Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.50A resolution Deposited 2020-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1244 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.281
6XHX Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with erythromycin and protein Y (YfiA) at 2.55A resolution Deposited 2020-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1479 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.272
6XHX Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with erythromycin and protein Y (YfiA) at 2.55A resolution Deposited 2020-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 995 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.272
6XHY Crystal structure of the Thermus thermophilus 70S ribosome in complex with telithromycin, mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.60A resolution Deposited 2020-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1510 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.266
6XHY Crystal structure of the Thermus thermophilus 70S ribosome in complex with telithromycin, mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.60A resolution Deposited 2020-06-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1165 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.266
6XQD Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UUC-mRNA, and deacylated P-site tRNA at 2.80A resolution Deposited 2020-07-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1535 ZN ZINC ION × 6 V7A Sarecycline × 1 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.276
6XQD Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UUC-mRNA, and deacylated P-site tRNA at 2.80A resolution Deposited 2020-07-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1164 ZN ZINC ION × 6 V7A Sarecycline × 1 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.276
6XQE Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UAA-mRNA, and deacylated P-site tRNA at 3.00A resolution Deposited 2020-07-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1465 K POTASSIUM ION × 1 ZN ZINC ION × 6 V7A Sarecycline × 1 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.00 Å R-free 0.311
6XQE Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UAA-mRNA, and deacylated P-site tRNA at 3.00A resolution Deposited 2020-07-09 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1173 K POTASSIUM ION × 1 ZN ZINC ION × 6 V7A Sarecycline × 1 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.00 Å R-free 0.311
7AZO 70S thermus thermophilus ribosome with bound antibiotic lead SEQ-977 Deposited 2020-11-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 56-meric
Chain L34A 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 448 OHX osmium (III) hexammine × 318 K POTASSIUM ION × 159 SJE (2R,3S,4R,5R,7S,9S,10S,11R,12S,13R)-12-(((2R,4R,5S,6S)-4,5-dihydroxy-4,6-dimethyltetrahydro-2H-pyran-2-yl)oxy)-2-((S)-1-(((2R,3R,4R,5R,6R)-5-hydroxy-3,4-dimethoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)propan-2-yl)-10-(((2S,3R,4R,6R)-3-hydroxy-4-(methoxyamino)-6-methyltetrahydro-2H-pyran-2-yl)oxy)-3,5,7,9,11,13-hexamethyl-7-(((2-((2-nitrophenyl)sulfonamido)ethyl)carbamoyl)oxy)-6,14-dioxooxacyclotetradecan-4-yl 3-methylbutanoate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；PEG2000MMES, KSCN
Resolution 3.30 Å R-free 0.238
7AZO 70S thermus thermophilus ribosome with bound antibiotic lead SEQ-977 Deposited 2020-11-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain L34B 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 340 OHX osmium (III) hexammine × 301 K POTASSIUM ION × 128 SJE (2R,3S,4R,5R,7S,9S,10S,11R,12S,13R)-12-(((2R,4R,5S,6S)-4,5-dihydroxy-4,6-dimethyltetrahydro-2H-pyran-2-yl)oxy)-2-((S)-1-(((2R,3R,4R,5R,6R)-5-hydroxy-3,4-dimethoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)propan-2-yl)-10-(((2S,3R,4R,6R)-3-hydroxy-4-(methoxyamino)-6-methyltetrahydro-2H-pyran-2-yl)oxy)-3,5,7,9,11,13-hexamethyl-7-(((2-((2-nitrophenyl)sulfonamido)ethyl)carbamoyl)oxy)-6,14-dioxooxacyclotetradecan-4-yl 3-methylbutanoate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；PEG2000MMES, KSCN
Resolution 3.30 Å R-free 0.238
7AZS 70S thermus thermophilus ribosome with bound antibiotic lead SEQ-569 Deposited 2020-11-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 56-meric
Chain L34A 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 450 K POTASSIUM ION × 159 OHX osmium (III) hexammine × 304 SJH (2R,3S,4R,5R,7S,9S,10S,11R,12S,13R)-12-(((2R,4R,5S,6S)-4,5-dihydroxy-4,6-dimethyltetrahydro-2H-pyran-2-yl)oxy)-2-((S)-1-(((2R,3R,4R,5R,6R)-5-hydroxy-3,4-dimethoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)propan-2-yl)-10-(((2S,3R,6R,E)-3-hydroxy-4-(methoxyimino)-6-methyltetrahydro-2H-pyran-2-yl)oxy)-3,5,7,9,11,13-hexamethyl-7-(((2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethyl)carbamoyl)oxy)-6,14-dioxooxacyclotetradecan-4-yl 3-methylbutanoate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；PEGMMES 2000, KSCN, Tris-HAc
Resolution 3.10 Å R-free 0.275
7AZS 70S thermus thermophilus ribosome with bound antibiotic lead SEQ-569 Deposited 2020-11-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain L34B 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 340 K POTASSIUM ION × 129 OHX osmium (III) hexammine × 294 SJH (2R,3S,4R,5R,7S,9S,10S,11R,12S,13R)-12-(((2R,4R,5S,6S)-4,5-dihydroxy-4,6-dimethyltetrahydro-2H-pyran-2-yl)oxy)-2-((S)-1-(((2R,3R,4R,5R,6R)-5-hydroxy-3,4-dimethoxy-6-methyltetrahydro-2H-pyran-2-yl)oxy)propan-2-yl)-10-(((2S,3R,6R,E)-3-hydroxy-4-(methoxyimino)-6-methyltetrahydro-2H-pyran-2-yl)oxy)-3,5,7,9,11,13-hexamethyl-7-(((2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethyl)carbamoyl)oxy)-6,14-dioxooxacyclotetradecan-4-yl 3-methylbutanoate × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；293 K；PEGMMES 2000, KSCN, Tris-HAc
Resolution 3.10 Å R-free 0.275
7JQL Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-001, mRNA, and deacylated P-site tRNA at 3.00A resolution Deposited 2020-08-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1440 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.00 Å R-free 0.285
7JQL Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-001, mRNA, and deacylated P-site tRNA at 3.00A resolution Deposited 2020-08-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1108 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.00 Å R-free 0.285
7JQM Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-002, mRNA, and deacylated P-site tRNA at 3.05A resolution Deposited 2020-08-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1174 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.05 Å R-free 0.283
7JQM Crystal structure of the Thermus thermophilus 70S ribosome in complex with Bac7-002, mRNA, and deacylated P-site tRNA at 3.05A resolution Deposited 2020-08-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 835 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.05 Å R-free 0.283
7LH5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with plazomicin, mRNA and tRNAs Deposited 2021-01-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain B7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 743 EDS (2S)-4-amino-N-[(1R,2S,3S,4R,5S)-5-amino-4-{[(2S,3R)-3-amino-6-{[(2-hydroxyethyl)amino]methyl}-3,4-dihydro-2H-pyran-2-y l]oxy}-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]-2-hydroxybutanamide × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；294.15 K；100 mM TRIS-HCl, pH 7.6, 3-3.2% (w/v) PEG 20K, 7-12% (v/v) MPD, 100-200 mM arginine, 0.5 mM Beta-mercaptoethanol
Resolution 3.27 Å R-free 0.277
7LH5 Crystal structure of the Thermus thermophilus 70S ribosome in complex with plazomicin, mRNA and tRNAs Deposited 2021-01-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain D7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 633 EDS (2S)-4-amino-N-[(1R,2S,3S,4R,5S)-5-amino-4-{[(2S,3R)-3-amino-6-{[(2-hydroxyethyl)amino]methyl}-3,4-dihydro-2H-pyran-2-y l]oxy}-2-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-3-hydroxycyclohexyl]-2-hydroxybutanamide × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；294.15 K；100 mM TRIS-HCl, pH 7.6, 3-3.2% (w/v) PEG 20K, 7-12% (v/v) MPD, 100-200 mM arginine, 0.5 mM Beta-mercaptoethanol
Resolution 3.27 Å R-free 0.277
7MD7 Crystal structure of the Thermus thermophilus 70S ribosome in complex with triphenylphosphonium analog of chloramphenicol CAM-C4-TPP and protein Y (YfiA) at 2.80A resolution Deposited 2021-04-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1514 YXM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-5-(triphenyl-lambda~5~-phosphanyl)pentanamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.268
7MD7 Crystal structure of the Thermus thermophilus 70S ribosome in complex with triphenylphosphonium analog of chloramphenicol CAM-C4-TPP and protein Y (YfiA) at 2.80A resolution Deposited 2021-04-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1040 YXM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-5-(triphenyl-lambda~5~-phosphanyl)pentanamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.268
7RQ8 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.50A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1567 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.268
7RQ8 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.50A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1002 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.268
7RQ9 Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.60A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1544 K POTASSIUM ION × 1 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.298
7RQ9 Crystal structure of the A2058-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAs, and aminoacylated P-site tRNA at 2.60A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1203 K POTASSIUM ION × 1 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.298
7RQA Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MTI-tripeptidyl-tRNA analog ACCA-ITM at 2.40A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1495 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.289
7RQA Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MTI-tripeptidyl-tRNA analog ACCA-ITM at 2.40A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1016 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 MRD (4R)-2-METHYLPENTANE-2,4-DIOL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.289
7RQB Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MAI-tripeptidyl-tRNA analog ACCA-IAM at 2.45A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1468 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.45 Å R-free 0.244
7RQB Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MAI-tripeptidyl-tRNA analog ACCA-IAM at 2.45A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1000 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.45 Å R-free 0.244
7RQC Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MFI-tripeptidyl-tRNA analog ACCA-IFM at 2.50A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1490 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.278
7RQC Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site MFI-tripeptidyl-tRNA analog ACCA-IFM at 2.50A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1008 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.278
7RQD Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MTI-tripeptidyl-tRNA analog ACCA-ITM, and chloramphenicol at 2.50A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1482 CLM CHLORAMPHENICOL × 1 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.280
7RQD Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MTI-tripeptidyl-tRNA analog ACCA-ITM, and chloramphenicol at 2.50A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1004 CLM CHLORAMPHENICOL × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.280
7RQE Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MAI-tripeptidyl-tRNA analog ACCA-IAM, and chloramphenicol at 2.40A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1474 CLM CHLORAMPHENICOL × 1 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.269
7RQE Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site deacylated tRNA analog CACCA, P-site MAI-tripeptidyl-tRNA analog ACCA-IAM, and chloramphenicol at 2.40A resolution Deposited 2021-08-06 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1019 CLM CHLORAMPHENICOL × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.269
7U2H Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, and deacylated E-site tRNAgly at 2.55A resolution Deposited 2022-02-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1547 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.263
7U2H Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, and deacylated E-site tRNAgly at 2.55A resolution Deposited 2022-02-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1199 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.263
7U2I Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution Deposited 2022-02-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1547 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.268
7U2I Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, aminoacylated P-site fMet-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution Deposited 2022-02-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1196 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.268
7U2J Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, peptidyl P-site fMAC-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution Deposited 2022-02-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1530 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.256
7U2J Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Gly-NH-tRNAgly, peptidyl P-site fMAC-NH-tRNAmet, deacylated E-site tRNAgly, and chloramphenicol at 2.55A resolution Deposited 2022-02-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1121 K POTASSIUM ION × 1 CLM CHLORAMPHENICOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M arginine-HCl, 0.1 M Tris-HCl, pH 7.6, 2.5% PEG20000, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.256
8CVJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMSEAC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2022-05-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1545 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.295
8CVJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMSEAC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2022-05-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1217 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.295
8CVK Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2022-05-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1542 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.284
8CVK Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2022-05-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1226 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.284
8CVL Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMTHSMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.30A resolution Deposited 2022-05-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1534 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.30 Å R-free 0.281
8CVL Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-NH-tRNAphe, peptidyl P-site fMTHSMRC-NH-tRNAmet, and deacylated E-site tRNAphe at 2.30A resolution Deposited 2022-05-18 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1189 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.30 Å R-free 0.281
8EKB Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, deacylated P-site tRNAmet, and thermorubin at 2.70A resolution Deposited 2022-09-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1469 K POTASSIUM ION × 1 T8B Thermorubin × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.257
8EKB Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, deacylated P-site tRNAmet, and thermorubin at 2.70A resolution Deposited 2022-09-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 54-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 994 K POTASSIUM ION × 1 T8B Thermorubin × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.257
8EV6 Crystal structure of the Thermus thermophilus 70S ribosome in complex with amikacin, mRNA, and A-, P-, and E-site tRNAs Deposited 2022-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1382 AKN (2S)-N-[(1R,2S,3S,4R,5S)-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-5-azanyl-2-[(2S,3R,4S,5S ,6R)-4-azanyl-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-cyclohexyl]-4-azanyl-2-oxidanyl-butanamide × 5 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.15 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 9-10.5% MPD, 0.5 mM BME
Resolution 2.95 Å R-free 0.272
8EV6 Crystal structure of the Thermus thermophilus 70S ribosome in complex with amikacin, mRNA, and A-, P-, and E-site tRNAs Deposited 2022-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 951 AKN (2S)-N-[(1R,2S,3S,4R,5S)-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-5-azanyl-2-[(2S,3R,4S,5S ,6R)-4-azanyl-6-(hydroxymethyl)-3,5-bis(oxidanyl)oxan-2-yl]oxy-3-oxidanyl-cyclohexyl]-4-azanyl-2-oxidanyl-butanamide × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.15 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 9-10.5% MPD, 0.5 mM BME
Resolution 2.95 Å R-free 0.272
8EV7 Crystal structure of the Thermus thermophilus 70S ribosome in complex with kanamycin, mRNA, and A-, P-, and E-site tRNAs Deposited 2022-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1416 KAN KANAMYCIN A × 4 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.15 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 9-10.5% MPD, 0.5 mM BME
Resolution 2.89 Å R-free 0.263
8EV7 Crystal structure of the Thermus thermophilus 70S ribosome in complex with kanamycin, mRNA, and A-, P-, and E-site tRNAs Deposited 2022-10-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 843 KAN KANAMYCIN A × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.15 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.9% PEG-20K, 9-10.5% MPD, 0.5 mM BME
Resolution 2.89 Å R-free 0.263
8FC1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.50A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1505 HGR Hygromycin A × 1 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.246
8FC1 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.50A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1002 HGR Hygromycin A × 1 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.246
8FC2 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.50A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1491 HGR Hygromycin A × 1 ZIT AZITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.250
8FC2 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.50A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1007 HGR Hygromycin A × 1 ZIT AZITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.250
8FC3 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.60A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1486 HGR Hygromycin A × 1 TEL TELITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.245
8FC3 Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.60A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1011 HGR Hygromycin A × 1 TEL TELITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.245
8FC4 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.45A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1487 HGR Hygromycin A × 1 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.45 Å R-free 0.264
8FC4 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.45A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 928 HGR Hygromycin A × 1 ERY ERYTHROMYCIN A × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.45 Å R-free 0.264
8FC5 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.65A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1500 HGR Hygromycin A × 1 ZIT AZITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.65 Å R-free 0.236
8FC5 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.65A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 966 HGR Hygromycin A × 1 ZIT AZITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.65 Å R-free 0.236
8FC6 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.35A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1487 HGR Hygromycin A × 1 TEL TELITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.35 Å R-free 0.248
8FC6 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.35A resolution Deposited 2022-12-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1009 HGR Hygromycin A × 1 TEL TELITHROMYCIN × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.35 Å R-free 0.248
8FOM Crystal structure of tRNA^Lys(SUU) bound to UAA codon in the ribosomal P site Deposited 2023-01-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 401 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.58 Å R-free 0.255
8FOM Crystal structure of tRNA^Lys(SUU) bound to UAA codon in the ribosomal P site Deposited 2023-01-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 441 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.58 Å R-free 0.255
8FON Crystal structure of tRNA^Lys(SUU) bound to AUA codon in the ribosomal P site Deposited 2023-01-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain R7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 586 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.64 Å R-free 0.278
8FON Crystal structure of tRNA^Lys(SUU) bound to AUA codon in the ribosomal P site Deposited 2023-01-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain Y7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 653 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 6 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP
Resolution 3.64 Å R-free 0.278
8G29 Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1559 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.252
8G29 Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 897 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.252
8G2A Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, peptidyl P-site fMTHSMRC-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1573 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.45 Å R-free 0.249
8G2A Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, peptidyl P-site fMTHSMRC-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1176 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.45 Å R-free 0.249
8G2B Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAphe, and aminoacylated P-site fMet-tRNAmet at 2.55A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1532 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.262
8G2B Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAphe, and aminoacylated P-site fMet-tRNAmet at 2.55A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1164 6IF methyl 7-chloro-6,7,8-trideoxy-6-{[(4S,5aS,8S,8aR)-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carbonyl]amino}-1-thio-L-threo-alpha-D-galacto-octopyranoside × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.262
8G2C Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.65A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1533 K POTASSIUM ION × 1 TYK TYLOSIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.65 Å R-free 0.249
8G2C Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.65A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1135 TYK TYLOSIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.65 Å R-free 0.249
8G2D Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.70A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1554 TYK TYLOSIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.277
8G2D Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.70A resolution Deposited 2023-02-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1235 TYK TYLOSIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.277
8T8B Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site formyl-MAI-tripeptidyl-tRNA analog ACCA-IAMf at 2.65A resolution Deposited 2023-06-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 102 PDB declaration: 112-meric
Chain 17 1–49（49 aa）
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 2428 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 6 ARG ARGININE × 2 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.65 Å R-free 0.257
8T8C Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site formyl-MFI-tripeptidyl-tRNA analog ACCA-IFMf at 2.60A resolution Deposited 2023-06-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 102 PDB declaration: 112-meric
Chain 17 1–49（49 aa）
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 2490 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 7 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.269
8UD6 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1487 K POTASSIUM ION × 1 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.241
8UD6 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1084 K POTASSIUM ION × 1 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.241
8UD7 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1521 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.242
8UD7 Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1143 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.242
8UD8 Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1546 K POTASSIUM ION × 1 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.261
8UD8 Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.70A resolution Deposited 2023-09-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1199 K POTASSIUM ION × 1 WC9 (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-thiabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.261
8UVR Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.60A resolution Deposited 2023-11-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1576 K POTASSIUM ION × 1 ZN ZINC ION × 6 SCM SPECTINOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.265
8UVR Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.60A resolution Deposited 2023-11-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1250 K POTASSIUM ION × 1 ZN ZINC ION × 6 SCM SPECTINOMYCIN × 1 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.265
8UVS Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin derivative 2694, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.75A resolution Deposited 2023-11-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1611 ZN ZINC ION × 6 Y7K (2R,4R,4aS,5aR,6S,7S,8R,9S,9aR,10aS)-2-methyl-6,8-bis(methylamino)-4-({[2-(oxan-4-yl)ethyl]amino}methyl)octahydro-2H-pyrano[2,3-b][1,4]benzodioxine-4,4a,7,9(10aH)-tetrol × 1 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.75 Å R-free 0.261
8UVS Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with spectinomycin derivative 2694, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.75A resolution Deposited 2023-11-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1250 ZN ZINC ION × 6 Y7K (2R,4R,4aS,5aR,6S,7S,8R,9S,9aR,10aS)-2-methyl-6,8-bis(methylamino)-4-({[2-(oxan-4-yl)ethyl]amino}methyl)octahydro-2H-pyrano[2,3-b][1,4]benzodioxine-4,4a,7,9(10aH)-tetrol × 1 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.75 Å R-free 0.261
8VTU Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-66, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1568 K POTASSIUM ION × 1 A1AEZ 1-cyclopropyl-7-[(4-{[3-({(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-3a,7,9,11,13,15-hexamethyl-14-[({3-[5-(methylcarbamoyl)pyridin-3-yl]prop-2-yn-1-yl}oxy)imino]-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl}oxy)-3-oxopropyl]amino}butyl)amino]-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.260
8VTU Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-66, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1187 K POTASSIUM ION × 1 A1AEZ 1-cyclopropyl-7-[(4-{[3-({(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-3a,7,9,11,13,15-hexamethyl-14-[({3-[5-(methylcarbamoyl)pyridin-3-yl]prop-2-yn-1-yl}oxy)imino]-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl}oxy)-3-oxopropyl]amino}butyl)amino]-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.260
8VTV Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-91, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1554 K POTASSIUM ION × 1 A1AE0 1-cyclopropyl-7-{7-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]hept-1-yn-1-yl}-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.262
8VTV Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-91, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1218 K POTASSIUM ION × 1 A1AE0 1-cyclopropyl-7-{7-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]hept-1-yn-1-yl}-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.262
8VTW Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-128 and protein Y at 2.35A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1450 A1AE1 1-cyclopropyl-7-(4-{4-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]butyl}piperazin-1-yl)-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ARG ARGININE × 2 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.35 Å R-free 0.243
8VTW Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-128 and protein Y at 2.35A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 984 A1AE1 1-cyclopropyl-7-(4-{4-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]butyl}piperazin-1-yl)-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.35 Å R-free 0.243
8VTX Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with macrolone MCX-128, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1548 K POTASSIUM ION × 1 A1AE1 1-cyclopropyl-7-(4-{4-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]butyl}piperazin-1-yl)-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.260
8VTX Crystal structure of the A2058-N6-dimethylated Thermus thermophilus 70S ribosome in complex with macrolone MCX-128, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.40A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1185 K POTASSIUM ION × 1 A1AE1 1-cyclopropyl-7-(4-{4-[({[(3aR,4R,7R,8S,9S,10R,11R,13R,14E,15S,15aR)-10-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-4-ethyl-11-methoxy-14-(methoxyimino)-3a,7,9,11,13,15-hexamethyl-2,6-dioxododecahydro-2H,4H-[1,3]dioxolo[4,5-c]oxacyclotetradecin-8-yl]oxy}carbonyl)amino]butyl}piperazin-1-yl)-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 PHE PHENYLALANINE × 1 FME N-FORMYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.260
8VTY Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ciprofloxacin and protein Y at 2.60A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1526 CPF 1-CYCLOPROPYL-6-FLUORO-4-OXO-7-PIPERAZIN-1-YL-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC ACID × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.241
8VTY Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ciprofloxacin and protein Y at 2.60A resolution Deposited 2024-01-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1021 CPF 1-CYCLOPROPYL-6-FLUORO-4-OXO-7-PIPERAZIN-1-YL-1,4-DIHYDROQUINOLINE-3-CARBOXYLIC ACID × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.241
8WV1 Ambient Temperature Structure of 50S Ribosomal Subunit from Thermus Thermophilus Deposited 2023-10-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 58 PDB declaration: 62-meric
Chain 2 1–49（49 aa）
Chain 7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 3807 ZN ZINC ION × 10 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；277 K；20% w/v PEG 3350 200 mM ammonium sulfate tribasic
Resolution 3.99 Å R-free 0.362
9B00 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with berberine analog of chloramphenicol CAM-BER, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.80A resolution Deposited 2024-03-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1524 K POTASSIUM ION × 1 A1AIX 13-(2-{[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]amino}-2-oxoethyl)-9,10-dimethoxy-5,6-dihydro-2H-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.284
9B00 Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with berberine analog of chloramphenicol CAM-BER, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.80A resolution Deposited 2024-03-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1199 K POTASSIUM ION × 1 A1AIX 13-(2-{[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]amino}-2-oxoethyl)-9,10-dimethoxy-5,6-dihydro-2H-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.284
9D0G Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with O-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1553 K POTASSIUM ION × 1 A1A1F (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1S,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-2,13-dioxabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.265
9D0G Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with O-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1239 K POTASSIUM ION × 1 A1A1F (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1S,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-2,13-dioxabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.265
9D0H Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with C-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1549 K POTASSIUM ION × 1 A1A1J (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,2R,3R,4Z,9R,10R,11R,12R)-10,11,12-trihydroxy-3-methyl-13-oxabicyclo[7.3.1]tridec-4-en-2-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.266
9D0H Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with C-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1241 K POTASSIUM ION × 1 A1A1J (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,2R,3R,4Z,9R,10R,11R,12R)-10,11,12-trihydroxy-3-methyl-13-oxabicyclo[7.3.1]tridec-4-en-2-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.266
9D0I Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Se-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2024-08-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1551 K POTASSIUM ION × 1 A1A1K (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-selenabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.45 Å R-free 0.265
9D0I Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Se-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution Deposited 2024-08-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1244 K POTASSIUM ION × 1 A1A1K (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1R,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-13-oxa-2-selenabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.45 Å R-free 0.265
9D0J Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with BT-33, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1475 A1A1L (4S,5aS,8S,8aR)-N-[(1R,4R,6Z,8R,9R,10R,11R,12S,13R)-4-fluoro-11,12,13-trihydroxy-8-methyl-14-oxa-2-thiabicyclo[8.3.1]tetradec-6-en-9-yl]-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.260
9D0J Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with BT-33, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1089 A1A1L (4S,5aS,8S,8aR)-N-[(1R,4R,6Z,8R,9R,10R,11R,12S,13R)-4-fluoro-11,12,13-trihydroxy-8-methyl-14-oxa-2-thiabicyclo[8.3.1]tetradec-6-en-9-yl]-4-(2-methylpropyl)octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name) × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.260
9D7R Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Fva1 antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.70A resolution Deposited 2024-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1123 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.275
9D7R Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Fva1 antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.70A resolution Deposited 2024-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 885 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.275
9D7S Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Api antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.85A resolution Deposited 2024-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1101 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.85 Å R-free 0.268
9D7S Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Api antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.85A resolution Deposited 2024-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 891 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.85 Å R-free 0.268
9D7T Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Api137 antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.70A resolution Deposited 2024-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1127 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.278
9D7T Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Api137 antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.70A resolution Deposited 2024-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 915 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.278
9DFC Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1554 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.265
9DFC Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Deposited 2024-08-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1250 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.50 Å R-free 0.265
9DFD Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin B, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.60A resolution Deposited 2024-08-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1554 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.266
9DFD Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin B, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.60A resolution Deposited 2024-08-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1250 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.266
9DFE Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin and protein Y at 2.60A resolution Deposited 2024-08-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1496 ARG ARGININE × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
9DFE Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with lasso peptide lariocidin and protein Y at 2.60A resolution Deposited 2024-08-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 54-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1024 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
9MTP Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.40A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1538 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.263
9MTP Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.40A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1138 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.40 Å R-free 0.263
9MTQ Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGD-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.55A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1527 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.257
9MTQ Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGD-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.55A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1151 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.55 Å R-free 0.257
9MTR Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGS-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.80A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1531 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.264
9MTR Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site GGS-mutant Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.80A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1118 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.264
9MTS Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-unmodified Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.70A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1465 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.262
9MTS Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-unmodified Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.70A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1124 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.262
9MTT Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site deacylated-tRNAcys at 2.60A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1539 K POTASSIUM ION × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.271
9MTT Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site deacylated-tRNAcys at 2.60A resolution Deposited 2025-01-12 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1148 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.271
9NO7 Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys at 2.13A resolution Deposited 2025-03-08 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 56-meric
Chain 7 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1685 K POTASSIUM ION × 52 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.6；5 mM HEPES-KOH, 50 mM KCl, 10 mM NH4Cl, 10 mM Mg(CH3COO)2
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.13 Å
9O3H Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.65A resolution Deposited 2025-04-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1542 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.65 Å R-free 0.276
9O3H Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.65A resolution Deposited 2025-04-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1207 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.65 Å R-free 0.276
9O3I Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ketolide telithromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.80A resolution Deposited 2025-04-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1518 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.281
9O3I Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with ketolide telithromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.80A resolution Deposited 2025-04-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1141 K POTASSIUM ION × 1 TEL TELITHROMYCIN × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.281
9O3J Crystal structure of the wild-type drug-free Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.60A resolution Deposited 2025-04-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 100 PDB declaration: 114-meric
Chain 17 1–49（49 aa）
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 2734 K POTASSIUM ION × 2 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.278
9O3K Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMAC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.70A resolution Deposited 2025-04-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 17 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 1529 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.262
9O3K Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, aminoacylated A-site Lys-tRNAlys, P-site fMAC-peptidyl-tRNAmet, and deacylated E-site tRNAlys at 2.70A resolution Deposited 2025-04-07 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 993 K POTASSIUM ION × 1 ERY ERYTHROMYCIN A × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.262
9O3L Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolide erythromycin, mRNA, deacylated A-site tRNAphe, P-site fMRC-peptidyl-tRNAmet, and deacylated E-site tRNAphe at 2.75A resolution Deposited 2025-04-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 100 PDB declaration: 114-meric
Chain 17 1–49（49 aa）
Chain 27 1–49（49 aa）
Not recorded MG MAGNESIUM ION × 2713 K POTASSIUM ION × 2 ERY ERYTHROMYCIN A × 2 ZN ZINC ION × 12 SF4 IRON/SULFUR CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.75 Å R-free 0.282

286 other PDB entries and 538 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL34_THET8
Isoform —
PDB entities 28
Chains and sequence ranges Author chain Z； PDBConstruct 1–49； UniProt 1–49

50S ribosomal protein L35

OrganismNot specified

UniProt Q72L77

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain a； UniProt 1–65 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain a 1–65（65 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B8 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D8 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain B8 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain D8 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B5 2–65（64 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain C5 2–65（64 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain B5 2–64（63 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain D5 2–64（63 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain B5 2–64（63 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain D5 2–64（63 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B8 2–65（64 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D8 2–65（64 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B8 2–65（64 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D8 2–65（64 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B8 2–65（64 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D8 2–65（64 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain B8 2–65（64 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain D8 2–65（64 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain B8 2–64（63 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain D8 2–64（63 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain A5 2–64（63 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain C5 2–64（63 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain B8 2–65（64 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain D8 2–65（64 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain Q8 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain M5 2–62（61 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL35 2–64（63 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BL35 2–64（63 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 18 2–65（64 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 28 2–65（64 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain Ae 1–64（64 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain Ae 1–64（64 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain BA 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain XB 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain Q8 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain M5 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain FA 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain KC 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 18 1–65（65 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 28 1–65（65 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain FA 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain JC 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain FA 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain KC 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain FA 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain IC 1–65（65 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL35 2–65（64 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL35 2–65（64 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

26 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL35_THET2
Isoform —
PDB entities 29
Chains and sequence ranges Author chain a； PDBConstruct 1–65； UniProt 1–65

50S ribosomal protein L36

OrganismNot specified

UniProt Q72I28

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain b； UniProt 1–37 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain b 1–37（37 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V68 T. thermophilus 70S ribosome in complex with mRNA, tRNAs and EF-Tu.GDP.kirromycin ternary complex, fitted to a 6.4 A Cryo-EM map. Deposited 2008-12-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 60-meric
Chain B9 2–36（35 aa）
Not recorded PHA PHENYLALANINAL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MAU N-METHYL KIRROMYCIN × 1 BME BETA-MERCAPTOETHANOL × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen METHANE；A Vitrobot was used to shock-freeze the sample in liquid methane.
Resolution 6.40 Å
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B9 1–37（37 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D9 1–37（37 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B9 1–37（37 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D9 1–37（37 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain B9 1–37（37 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain D9 1–37（37 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain B9 1–37（37 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain D9 1–37（37 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain B9 1–37（37 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain D9 1–37（37 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 19 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 29 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain Af 1–37（37 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 58-meric
Chain Af 1–37（37 aa）
Not recorded NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 5.00 Å
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain GA 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain LC 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 19 1–37（37 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 29 1–37（37 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain GA 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain KC 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain GA 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain LC 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain GA 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain JC 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL36 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BL36 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6Q95 Structure of tmRNA SmpB bound in A site of T. thermophilus 70S ribosome Deposited 2018-12-17 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 61-meric
Chain f 1–37（37 aa）
Not recorded MG MAGNESIUM ION × 637 ZN ZINC ION × 3 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.70 Å

17 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RL36_THET2
Isoform —
PDB entities 30
Chains and sequence ranges Author chain b； PDBConstruct 1–37； UniProt 1–37

30S ribosomal protein S2

OrganismNot specified

UniProt P62662

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain c； UniProt 1–256 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain B 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain c 1–256（256 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AB 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CB 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AB 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CB 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AB 7–240（234 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DB 7–240（234 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AB 7–240（234 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CB 7–240（234 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AB 7–240（234 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CB 7–240（234 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AB 7–241（235 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CB 7–241（235 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AB 7–241（235 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CB 7–241（235 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AB 7–241（235 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CB 7–241（235 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AB 7–241（235 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CB 7–241（235 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AB 7–240（234 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CB 7–240（234 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BB 7–240（234 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DB 7–240（234 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AB 7–241（235 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CB 7–241（235 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 1E 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 12 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 1E 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 12 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 1E 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 12 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 1E 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 12 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1E 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 12 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS02 7–240（234 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS02 7–240（234 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1b 7–237（231 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2b 7–237（231 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BF 7–240（234 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain CA 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain YB 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 1E 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 12 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain QB 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1425 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.04 Å R-free 0.279
5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain XB 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1011 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.04 Å R-free 0.279
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain KA 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain PC 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1b 1–256（256 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2b 1–256（256 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain KA 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain OC 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain LA 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain QC 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain JA 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain MC 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AB 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CB 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1b 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2b 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1b 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2b 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1b 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2b 1–256（256 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS02 2–256（255 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS02 2–256（255 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

35 other PDB entries and 67 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS2_THET2
Isoform —
PDB entities 35
Chains and sequence ranges Author chain c； PDBConstruct 1–256； UniProt 1–256

30S ribosomal protein S3

OrganismNot specified

UniProt P62663

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain d； UniProt 1–239 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain C 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V42 Crystal structure of the ribosome at 5.5 A resolution. Deposited 2001-03-30 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 42 PDB declaration: 49-meric
Chain AF 1–239（239 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 5.50 Å
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain d 1–239（239 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AC 2–208（207 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CC 2–208（207 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AC 2–208（207 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CC 2–208（207 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AC 2–208（207 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CC 2–208（207 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AC 2–208（207 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CC 2–208（207 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CC 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BC 2–207（206 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DC 2–207（206 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AC 2–208（207 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CC 2–208（207 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 2E 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 22 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 2E 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 22 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 2E 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 22 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 2E 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 22 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2E 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 22 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS03 2–207（206 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS03 2–207（206 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1c 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2c 2–207（206 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BG 2–207（206 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain DA 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain ZB 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 2E 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 22 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain QC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1425 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.04 Å R-free 0.279
5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain XC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1011 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.04 Å R-free 0.279
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain LA 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain QC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1c 1–239（239 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2c 1–239（239 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain LA 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain PC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain MA 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain RC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain KA 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain NC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CC 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1c 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2c 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1c 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2c 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1c 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2c 1–239（239 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS03 2–239（238 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS03 2–239（238 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

36 other PDB entries and 68 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS3_THET2
Isoform —
PDB entities 36
Chains and sequence ranges Author chain d； PDBConstruct 1–239； UniProt 1–239

30S ribosomal protein S4

OrganismNot specified

UniProt P62664

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain e； UniProt 1–209 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain D 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain e 1–209（209 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AD 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CD 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AD 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CD 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AD 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DD 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AD 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CD 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AD 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CD 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AD 2–209（208 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CD 2–209（208 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AD 2–209（208 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CD 2–209（208 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AD 2–209（208 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CD 2–209（208 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AD 2–209（208 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CD 2–209（208 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AD 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CD 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BD 2–209（208 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DD 2–209（208 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AD 2–209（208 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CD 2–209（208 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 3E 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 32 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 3E 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 32 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 3E 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 32 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 3E 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 32 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 3E 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 32 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS04 2–209（208 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS04 2–209（208 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1d 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2d 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BH 2–209（208 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain EA 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain AC 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 3E 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 32 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain MA 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain RC 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1d 1–209（209 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2d 1–209（209 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain MA 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain QC 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain NA 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain SC 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain LA 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain OC 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AD 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CD 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1d 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2d 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1d 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2d 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1d 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2d 1–209（209 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS04 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS04 2–209（208 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

34 other PDB entries and 65 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS4_THET2
Isoform —
PDB entities 37
Chains and sequence ranges Author chain e； PDBConstruct 1–209； UniProt 1–209

30S ribosomal protein S5

OrganismNot specified

UniProt P62665

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain f； UniProt 1–162 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain E 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain f 1–162（162 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AE 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CE 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AE 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CE 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AE 5–155（151 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DE 5–155（151 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AE 5–155（151 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CE 5–155（151 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AE 5–155（151 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CE 5–155（151 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AE 5–155（151 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CE 5–155（151 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AE 5–155（151 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CE 5–155（151 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AE 5–155（151 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CE 5–155（151 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AE 5–155（151 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CE 5–155（151 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AE 5–155（151 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CE 5–155（151 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BE 5–155（151 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DE 5–155（151 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AE 5–155（151 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CE 5–155（151 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 4E 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 42 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS05 5–155（151 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS05 5–155（151 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1e 5–152（148 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2e 5–152（148 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BI 5–154（150 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain FA 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain BC 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 4E 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 42 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain NA 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain SC 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1e 1–162（162 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2e 1–162（162 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain NA 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain RC 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain OA 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain TC 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain MA 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain PC 1–162（162 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6CAP Crystal Structure of 30S ribosomal subunit from Thermus thermophilus in complex with Sisomicin Deposited 2018-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain E 5–154（150 aa）
Not recorded MG MAGNESIUM ION × 252 SIS (1S,2S,3R,4S,6R)-4,6-diamino-3-{[(2S,3R)-3-amino-6-(aminomethyl)-3,4-dihydro-2H-pyran-2-yl]oxy}-2-hydroxycyclohexyl 3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranoside × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.5；298 K；17% MPD
Resolution 3.40 Å R-free 0.230
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS05 2–162（161 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS05 2–162（161 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

27 other PDB entries and 50 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS5_THET2
Isoform —
PDB entities 38
Chains and sequence ranges Author chain f； PDBConstruct 1–162； UniProt 1–162

30S ribosomal protein S6

OrganismNot specified

UniProt P62666

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain g； UniProt 1–101 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain F 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain QF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 325 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.278
4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain XF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 351 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.278
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain g 1–101（101 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AF 1–101（101 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CF 1–101（101 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AF 1–101（101 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CF 1–101（101 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AF 1–101（101 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CF 1–101（101 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AF 1–101（101 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CF 1–101（101 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CF 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BF 1–101（101 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DF 1–101（101 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AF 1–101（101 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CF 1–101（101 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 5E 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 52 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS06 1–101（101 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS06 1–101（101 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1f 1–100（100 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2f 1–100（100 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BJ 1–101（101 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain GA 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain CC 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 5E 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 52 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain OA 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain TC 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1f 1–101（101 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2f 1–101（101 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain OA 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain SC 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain PA 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain UC 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain NA 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain QC 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6I6I Circular permutant of ribosomal protein S6, adding 6aa to C terminal of P68-69, L75A mutant Deposited 2018-11-15 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 3–74（72 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Lithium sulphate 0.1 M Tris pH 8.5 40% v/v PEG 400
Resolution 1.50 Å R-free 0.233
6I6I Circular permutant of ribosomal protein S6, adding 6aa to C terminal of P68-69, L75A mutant Deposited 2018-11-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 3–74（72 aa）
Not recorded SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；0.2 M Lithium sulphate 0.1 M Tris pH 8.5 40% v/v PEG 400
Resolution 1.50 Å R-free 0.233
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS06 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS06 1–101（101 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
7B8V Circular permutant of ribosomal protein S6, P54-55 Deposited 2020-12-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain A 55–91（37 aa）
Chain A 3–54（52 aa）
Chain B 55–91（37 aa）
Chain B 3–54（52 aa）
Chain D 55–91（37 aa）
Chain D 3–54（52 aa）
Chain F 55–91（37 aa）
Chain F 3–54（52 aa）
Chain G 55–91（37 aa）
Chain G 3–54（52 aa）
Chain I 55–91（37 aa）
Chain I 3–54（52 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.2 M sodium bromide 0.1 M Bis-Tris propane pH 6.5 20% w/v PEG 3350
Resolution 1.86 Å R-free 0.286
7B8V Circular permutant of ribosomal protein S6, P54-55 Deposited 2020-12-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain O 55–91（37 aa）
Chain O 3–54（52 aa）
Chain S 55–91（37 aa）
Chain S 3–54（52 aa）
Chain T 55–91（37 aa）
Chain T 3–54（52 aa）
Chain U 55–91（37 aa）
Chain U 3–54（52 aa）
Chain W 55–91（37 aa）
Chain W 3–54（52 aa）
Chain X 55–91（37 aa）
Chain X 3–54（52 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.2 M sodium bromide 0.1 M Bis-Tris propane pH 6.5 20% w/v PEG 3350
Resolution 1.86 Å R-free 0.286
7B8V Circular permutant of ribosomal protein S6, P54-55 Deposited 2020-12-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain C 55–91（37 aa）
Chain C 3–54（52 aa）
Chain E 55–91（37 aa）
Chain E 3–54（52 aa）
Chain H 55–91（37 aa）
Chain H 3–54（52 aa）
Chain J 55–91（37 aa）
Chain J 3–54（52 aa）
Chain K 55–91（37 aa）
Chain K 3–54（52 aa）
Chain L 55–91（37 aa）
Chain L 3–54（52 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.2 M sodium bromide 0.1 M Bis-Tris propane pH 6.5 20% w/v PEG 3350
Resolution 1.86 Å R-free 0.286
7B8V Circular permutant of ribosomal protein S6, P54-55 Deposited 2020-12-13 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 4 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric
Chain M 55–91（37 aa）
Chain M 3–54（52 aa）
Chain N 55–91（37 aa）
Chain N 3–54（52 aa）
Chain P 55–91（37 aa）
Chain P 3–54（52 aa）
Chain Q 55–91（37 aa）
Chain Q 3–54（52 aa）
Chain R 55–91（37 aa）
Chain R 3–54（52 aa）
Chain V 55–91（37 aa）
Chain V 3–54（52 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.2 M sodium bromide 0.1 M Bis-Tris propane pH 6.5 20% w/v PEG 3350
Resolution 1.86 Å R-free 0.286

29 other PDB entries and 57 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS6_THET2
Isoform —
PDB entities 39
Chains and sequence ranges Author chain g； PDBConstruct 1–101； UniProt 1–101

30S ribosomal protein S7

OrganismNot specified

UniProt P62667

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain h； UniProt 1–156 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain G 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain h 1–156（156 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V5L The structure of EF-Tu and aminoacyl-tRNA bound to the 70S ribosome with a GTP analog Deposited 2010-09-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain AG 1–156（156 aa）
Not recorded PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, AND 5.3% (W/V) PEG20K
Resolution 3.10 Å R-free 0.268
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AG 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CG 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AG 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CG 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AG 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DG 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AG 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CG 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AG 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CG 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AG 2–156（155 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CG 2–156（155 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AG 2–156（155 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CG 2–156（155 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AG 2–156（155 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CG 2–156（155 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AG 2–156（155 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CG 2–156（155 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AG 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CG 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BG 2–156（155 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DG 2–156（155 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AG 2–156（155 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CG 2–156（155 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 6E 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 62 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 6E 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 62 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 6E 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 62 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 6E 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 62 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 6E 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 62 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS07 2–156（155 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS07 2–156（155 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1g 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2g 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BK 2–156（155 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain HA 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain DC 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 6E 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 62 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain PA 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain UC 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1g 1–156（156 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2g 1–156（156 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain PA 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain TC 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain QA 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain VC 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain OA 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain RC 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AG 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CG 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1g 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2g 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1g 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2g 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1g 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2g 1–156（156 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS07 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS07 2–156（155 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

35 other PDB entries and 66 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS7_THET2
Isoform —
PDB entities 40
Chains and sequence ranges Author chain h； PDBConstruct 1–156； UniProt 1–156

30S ribosomal protein S8

OrganismNot specified

UniProt P62668

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain i； UniProt 1–138 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1XMO Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center Deposited 2004-10-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain H 1–138（138 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 107 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, magnesium chloride, potassium chloride, ammonium chloride, MES-KOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.K
Resolution 3.25 Å R-free 0.284
1XMQ Crystal Structure of t6A37-ASLLysUUU AAA-mRNA Bound to the Decoding Center Deposited 2004-10-04 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain H 1–138（138 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 107 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, magnesium chloride, potassium chloride, ammonium chloride, MES-KOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.K
Resolution 3.00 Å R-free 0.236
1XNQ Structure of an Inosine-Adenine Wobble Base Pair Complex in the Context of the Decoding Center Deposited 2004-10-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain H 1–138（138 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 107 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, magnesium chloride, potassium chloride, ammonium chloride, MES-KOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.K
Resolution 3.05 Å R-free 0.270
1XNR Crystal Structure of an Inosine-Cytosine Wobble Base Pair in the Context of the Decoding Center Deposited 2004-10-05 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain H 1–138（138 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 108 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, magnesium chloride, potassium chloride, ammonium chloride, MES-KOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.K
Resolution 3.10 Å R-free 0.273
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain H 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain i 1–138（138 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AH 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CH 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AH 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CH 1–138（138 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AH 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CH 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AH 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CH 1–138（138 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CH 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BH 1–138（138 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DH 1–138（138 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AH 1–138（138 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CH 1–138（138 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 7E 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 72 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS08 1–138（138 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS08 1–138（138 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1h 2–138（137 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2h 2–138（137 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BL 1–138（138 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain IA 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain EC 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 7E 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 72 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain QA 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain VC 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1h 1–138（138 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2h 1–138（138 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain QA 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain UC 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain RA 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain WC 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain PA 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain SC 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1h 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2h 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS08 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS08 1–138（138 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

31 other PDB entries and 55 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS8_THET2
Isoform —
PDB entities 41
Chains and sequence ranges Author chain i； PDBConstruct 1–138； UniProt 1–138

30S ribosomal protein S9

OrganismNot specified

UniProt P62669

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain j； UniProt 1–128 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2E5L A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction Deposited 2006-12-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain I 1–128（128 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.8；293 K；10mM HEPES-KOH, 10mM MgCl2, 60mM NH4Cl, 6mM beta-mercaptoethanol, 11-12% MPD, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.30 Å R-free 0.301
2F4V 30S ribosome + designer antibiotic Deposited 2005-11-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain I 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 101 K POTASSIUM ION × 12 D2C (2S,4S,4AR,5AS,6S,11R,11AS,12R,12AR)-7-CHLORO-4-(DIMETHYLAMINO)-6,10,11,12-TETRAHYDROXY-1,3-DIOXO-1,2,3,4,4A,5,5A,6,11,11A,12,12A-DODECAHYDROTETRACENE-2-CARBOXAMIDE × 1 AB9 (2R)-4-AMINO-N-{(1R,2S,3R,4R,5S)-5-AMINO-2-{2-[(2-AMINOETHYL)AMINO]ETHOXY}-4-[(2,6-DIAMINO-2,6-DIDEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-3-HYDROXYCYCLOHEXYL}-2-HYDROXYBUTANAMIDE × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.80 Å R-free 0.315
2R1G Coordinates of the thermus thermophilus 30S components neighboring RbfA as obtained by fitting into the CRYO-EM map of A 30S-RBFA complex Deposited 2007-08-22 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 3 PDB declaration: decameric
Chain G 2–128（127 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20mM, Hepes-KOH (pH 7.8), 10mM Mg(OAc)2, 200mM NH4Cl, 65mM KCl；pH 7.8；20mM, Hepes-KOH (pH 7.8), 10mM Mg(OAc)2, 200mM NH4Cl, 65mM KCl
cryo-EM vitrification conditions Cryogen ETHANE；RAPID-FREEZING IN LIQUID ETHANE
Resolution 12.50 Å
2UUA Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a Valine-ASL with cmo5U in position 34 bound to an mRNA with a GUC-codon in the A-site and paromomycin. Deposited 2007-03-01 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain I 1–128（128 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 170 K POTASSIUM ION × 22 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；SEE 1J5E, pH 6.5
Resolution 2.90 Å R-free 0.254
2VQE Modified uridines with C5-methylene substituents at the first position of the tRNA anticodon stabilize U-G wobble pairing during decoding Deposited 2008-03-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain I 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 206 K POTASSIUM ION × 62 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；SEE WIMBERLY ET AL., NATURE 2000, pH 6.5
Resolution 2.50 Å R-free 0.283
2VQF Modified uridines with C5-methylene substituents at the first position of the tRNA anticodon stabilize U-G wobble pairing during decoding Deposited 2008-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain I 1–128（128 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 214 K POTASSIUM ION × 73 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；SEE WIMBERLY ET AL.,NATURE, 2000, pH 6.5
Resolution 2.90 Å R-free 0.258
2ZM6 Crystal structure of the Thermus thermophilus 30S ribosomal subunit Deposited 2008-04-11 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 21-meric
Chain I 1–128（128 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.8；298 K；10mM HEPES-KOH, 10mM MgCl2, 60mM NH4Cl, 6mM beta-mercaptoethanol, 11-12% MPD, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.30 Å R-free 0.323
3T1H Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human anti-codon stem loop (HASL) of transfer RNA lysine 3 (tRNALys3) bound to an mRNA with an AAA-codon in the A-site and Paromomycin Deposited 2011-07-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain I 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 185 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.11 Å R-free 0.276
3T1Y Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human anti-codon stem loop (HASL) of transfer RNA Lysine 3 (TRNALYS3) bound to an mRNA with an AAG-codon in the A-site and paromomycin Deposited 2011-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain I 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 186 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.268
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain I 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain j 1–128（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V4R Crystal structure of the whole ribosomal complex. Deposited 2005-09-30 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AI 1–128（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；277 K；PEG20k, MES buffer, magnesium acetate, potassium chloride, ammonium chloride, pH 6.7, VAPOR DIFFUSION, temperature 277K
Resolution 5.90 Å R-free 0.371
4V4S Crystal structure of the whole ribosomal complex. Deposited 2005-10-12 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AI 1–128（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；277 K；PEG20k, MES buffer, magnesium acetate, potassium chloride, ammonium chloride, pH 6.7, VAPOR DIFFUSION, temperature 277K
Resolution 6.76 Å R-free 0.356
4V4T Crystal structure of the whole ribosomal complex with a stop codon in the A-site. Deposited 2005-10-12 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain AI 1–128（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；277 K；PEG20k, MES buffer, magnesium acetate, potassium chloride, ammonium chloride, pH 6.7, VAPOR DIFFUSION, temperature 277K
Resolution 6.46 Å R-free 0.361
4V51 Structure of the Thermus thermophilus 70S ribosome complexed with mRNA, tRNA and paromomycin Deposited 2006-07-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；0.2M KSCN, 4% PEG 20K, 4% PEG550 MME, 0.1M TRIS-ACETATE PH 7
Resolution 2.80 Å R-free 0.313
4V51 Structure of the Thermus thermophilus 70S ribosome complexed with mRNA, tRNA and paromomycin Deposited 2006-07-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain CI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 638 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；0.2M KSCN, 4% PEG 20K, 4% PEG550 MME, 0.1M TRIS-ACETATE PH 7
Resolution 2.80 Å R-free 0.313
4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 741 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
Resolution 3.50 Å R-free 0.326
4V5A Structure of the Ribosome Recycling Factor bound to the Thermus thermophilus 70S ribosome with mRNA, ASL-Phe and tRNA-fMet Deposited 2007-06-28 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 741 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；COMPARE SELMER ET AL SCIENCE 2006, pH 7.0
Resolution 3.50 Å R-free 0.326
4V5C Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A-site tRNA, deacylated P-site tRNA, and E-site tRNA. Deposited 2009-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；pH 7.1
Resolution 3.30 Å R-free 0.272
4V5C Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A-site tRNA, deacylated P-site tRNA, and E-site tRNA. Deposited 2009-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain CI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 741 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；pH 7.1
Resolution 3.30 Å R-free 0.272
4V5D Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA. Deposited 2009-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 673 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；pH 7.1
Resolution 3.50 Å R-free 0.256
4V5D Structure of the Thermus thermophilus 70S ribosome in complex with mRNA, paromomycin, acylated A- and P-site tRNAs, and E-site tRNA. Deposited 2009-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 57-meric
Chain CI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 673 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；pH 7.1
Resolution 3.50 Å R-free 0.256
4V5E Insights into translational termination from the structure of RF2 bound to the ribosome Deposited 2009-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 546 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；SEE SELMER ET AL., SCIENCE 2006, pH 7.1
Resolution 3.45 Å R-free 0.257
4V5E Insights into translational termination from the structure of RF2 bound to the ribosome Deposited 2009-04-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 546 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.1；SEE SELMER ET AL., SCIENCE 2006, pH 7.1
Resolution 3.45 Å R-free 0.257
4V5J Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release Deposited 2010-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 548 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；SEE SELMER ET AL., SCIENCE 2006, pH 6.5
Resolution 3.10 Å R-free 0.264
4V5J Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release Deposited 2010-03-24 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 546 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；SEE SELMER ET AL., SCIENCE 2006, pH 6.5
Resolution 3.10 Å R-free 0.264
4V5K Structure of cytotoxic domain of colicin E3 bound to the 70S ribosome Deposited 2010-05-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1164 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.18；AS STATED IN STRUCTURE OF THE 70S RIBOSOME COMPLEXED WITH MRNA AND TRNA. (2006) SCIENCE 313: 1935, PH 7.18
Resolution 3.20 Å R-free 0.270
4V5K Structure of cytotoxic domain of colicin E3 bound to the 70S ribosome Deposited 2010-05-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 52 PDB declaration: 58-meric
Chain not uniquely mapped Reference range not declared
Not recorded MG MAGNESIUM ION × 1170 ZN ZINC ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.18；AS STATED IN STRUCTURE OF THE 70S RIBOSOME COMPLEXED WITH MRNA AND TRNA. (2006) SCIENCE 313: 1935, PH 7.18
Resolution 3.20 Å R-free 0.270
4V5L The structure of EF-Tu and aminoacyl-tRNA bound to the 70S ribosome with a GTP analog Deposited 2010-09-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain AI 1–128（128 aa）
Not recorded PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, AND 5.3% (W/V) PEG20K
Resolution 3.10 Å R-free 0.268
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V68 T. thermophilus 70S ribosome in complex with mRNA, tRNAs and EF-Tu.GDP.kirromycin ternary complex, fitted to a 6.4 A Cryo-EM map. Deposited 2008-12-11 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 60-meric
Chain AI 2–128（127 aa）
Not recorded PHA PHENYLALANINAL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MAU N-METHYL KIRROMYCIN × 1 BME BETA-MERCAPTOETHANOL × 1 ELECTRON MICROSCOPY
cryo-EM vitrification conditions Cryogen METHANE；A Vitrobot was used to shock-freeze the sample in liquid methane.
Resolution 6.40 Å
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AI 2–128（127 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DI 2–128（127 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AI 2–128（127 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CI 2–128（127 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AI 2–128（127 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CI 2–128（127 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AI 2–128（127 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CI 2–128（127 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AI 2–128（127 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CI 2–128（127 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AI 2–128（127 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CI 2–128（127 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AI 2–128（127 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CI 2–128（127 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AI 2–128（127 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CI 2–128（127 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BI 2–128（127 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DI 2–128（127 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Insufficient information Heteromer；Protein × 51 PDB declaration: 61-meric
Chain not uniquely mapped Reference range not declared
Not recorded PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME
Resolution 3.40 Å R-free 0.241
4WT8 Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex Deposited 2014-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Insufficient information Heteromer；Protein × 51 PDB declaration: 62-meric
Chain BI 2–128（127 aa）
Not recorded PAR PAROMOMYCIN × 1 3V6 Bactobolin A × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions EVAPORATION；293 K；0.2M KSCN, 0.1M Tris-HAc pH7, 3.5-4.5%(w/v) PEG20K, 3.5- 4.5%(w/v) PEG550MME
Resolution 3.40 Å R-free 0.241
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS09 2–128（127 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS09 2–128（127 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain JA 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain FC 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain RA 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain WC 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1i 1–128（128 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2i 1–128（128 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain RA 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain VC 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain SA 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain XC 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain QA 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain TC 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CI 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS09 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS09 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

44 other PDB entries and 72 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS9_THET2
Isoform —
PDB entities 42
Chains and sequence ranges Author chain j； PDBConstruct 1–128； UniProt 1–128

30S ribosomal protein S10

OrganismNot specified

UniProt P62653

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain k； UniProt 1–105 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain J 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain k 1–105（105 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AJ 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CJ 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AJ 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CJ 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AJ 3–100（98 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DJ 3–100（98 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AJ 3–100（98 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CJ 3–100（98 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AJ 3–100（98 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CJ 3–100（98 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AJ 3–101（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CJ 3–101（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AJ 3–101（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CJ 3–101（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AJ 3–101（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CJ 3–101（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AJ 3–101（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CJ 3–101（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AJ 3–100（98 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CJ 3–100（98 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BJ 3–100（98 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DJ 3–100（98 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AJ 3–101（99 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CJ 3–101（99 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 1I 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 1A 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS10 3–100（98 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS10 3–100（98 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1j 4–100（97 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2j 4–100（97 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BN 3–100（98 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain KA 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain GC 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 1I 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 1A 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain SA 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain XC 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1j 1–105（105 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2j 1–105（105 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain SA 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain WC 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain TA 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain YC 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain RA 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain UC 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS10 2–105（104 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS10 2–105（104 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

26 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS10_THET2
Isoform —
PDB entities 43
Chains and sequence ranges Author chain k； PDBConstruct 1–105； UniProt 1–105

30S ribosomal protein S11

OrganismNot specified

UniProt P62654

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain l； UniProt 1–129 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain K 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain l 1–129（129 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AK 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CK 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AK 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CK 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AK 11–129（119 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DK 11–129（119 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AK 11–129（119 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CK 11–129（119 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AK 11–129（119 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CK 11–129（119 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AK 11–129（119 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CK 11–129（119 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AK 11–129（119 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CK 11–129（119 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AK 11–129（119 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CK 11–129（119 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AK 11–129（119 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CK 11–129（119 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AK 11–124（114 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CK 11–124（114 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BK 11–124（114 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DK 11–124（114 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AK 11–129（119 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CK 11–129（119 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 2I 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 2A 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 2I 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 2A 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 2I 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 2A 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 2I 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 2A 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2I 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2A 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS11 11–124（114 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS11 11–124（114 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1k 13–126（114 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2k 13–126（114 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BO 11–129（119 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain LA 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain HC 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 2I 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 2A 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain TA 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain YC 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1k 1–129（129 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2k 1–129（129 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain TA 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain XC 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain UA 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain ZC 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain SA 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain VC 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AK 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CK 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS11 2–129（128 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS11 2–129（128 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 632 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 524 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 710 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2k 1–129（129 aa）
Not recorded MG MAGNESIUM ION × 629 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279

36 other PDB entries and 69 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS11_THET2
Isoform —
PDB entities 44
Chains and sequence ranges Author chain l； PDBConstruct 1–129； UniProt 1–129

30S ribosomal protein S12

OrganismNot specified

UniProt P61941

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain m； UniProt 1–132 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain L 1–132（132 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain m 1–132（132 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AL 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CL 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AL 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CL 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL 2–125（124 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DL 2–125（124 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL 2–125（124 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CL 2–125（124 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AL 2–125（124 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CL 2–125（124 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AL 2–126（125 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CL 2–126（125 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AL 2–126（125 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CL 2–126（125 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AL 2–126（125 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CL 2–126（125 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AL 2–126（125 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CL 2–126（125 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AL 2–123（122 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CL 2–123（122 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BL 2–123（122 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DL 2–123（122 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AL 2–126（125 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CL 2–126（125 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 3I 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 3A 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS12 2–123（122 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS12 2–123（122 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1l 2–123（122 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2l 2–123（122 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BP 2–125（124 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain MA 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain IC 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 3I 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 3A 1–132（132 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain UA 1–132（132 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ZC 1–132（132 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain UA 1–132（132 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain YC 1–132（132 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain VA 1–132（132 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain AD 1–132（132 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain TA 1–132（132 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain WC 1–132（132 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS12 2–132（131 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS12 2–132（131 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

25 other PDB entries and 47 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS12_THET2
Isoform —
PDB entities 45
Chains and sequence ranges Author chain m； PDBConstruct 1–132； UniProt 1–132

30S ribosomal protein S13

OrganismNot specified

UniProt P62655

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain n； UniProt 1–126 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2R1G Coordinates of the thermus thermophilus 30S components neighboring RbfA as obtained by fitting into the CRYO-EM map of A 30S-RBFA complex Deposited 2007-08-22 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 3 PDB declaration: decameric
Chain I 2–126（125 aa）
Not recorded No recorded non-water small molecule ELECTRON MICROSCOPY
cryo-EM buffer 20mM, Hepes-KOH (pH 7.8), 10mM Mg(OAc)2, 200mM NH4Cl, 65mM KCl；pH 7.8；20mM, Hepes-KOH (pH 7.8), 10mM Mg(OAc)2, 200mM NH4Cl, 65mM KCl
cryo-EM vitrification conditions Cryogen ETHANE；RAPID-FREEZING IN LIQUID ETHANE
Resolution 12.50 Å
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain M 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain n 1–126（126 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AM 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CM 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AM 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CM 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AM 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DM 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AM 2–117（116 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CM 2–117（116 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AM 2–117（116 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CM 2–117（116 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AM 2–126（125 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CM 2–126（125 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AM 2–126（125 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CM 2–126（125 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AM 2–126（125 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CM 2–126（125 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AM 2–126（125 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CM 2–126（125 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AM 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CM 2–118（117 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BM 2–117（116 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DM 2–118（117 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AM 2–126（125 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CM 2–126（125 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 4I 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 4A 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 4I 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 4A 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 4I 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 4A 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 4I 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 4A 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 4I 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 4A 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS13 2–118（117 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS13 2–118（117 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1m 2–117（116 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2m 2–117（116 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BQ 2–115（114 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain NA 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain JC 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 4I 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 4A 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain VA 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AD 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1m 1–126（126 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2m 1–126（126 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain VA 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ZC 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain WA 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain BD 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain UA 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain XC 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AM 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CM 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CAO Structure of the ribosomal decoding complex at ambient temperature Deposited 2018-01-31 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain M 2–119（118 aa）
Not recorded PAR PAROMOMYCIN × 6 K POTASSIUM ION × 37 MG MAGNESIUM ION × 303 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions MICROBATCH；pH 6.5；298 K；17% MPD
Resolution 3.45 Å R-free 0.272
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS13 2–126（125 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS13 2–126（125 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 632 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 524 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 710 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2m 1–126（126 aa）
Not recorded MG MAGNESIUM ION × 629 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279

38 other PDB entries and 71 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS13_THET2
Isoform —
PDB entities 46
Chains and sequence ranges Author chain n； PDBConstruct 1–126； UniProt 1–126

30S ribosomal protein S14 type Z

OrganismNot specified

UniProt P62656

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain o； UniProt 1–61 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain N 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain o 1–61（61 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AN 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CN 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AN 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CN 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AN 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DN 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AN 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CN 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AN 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CN 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AN 2–61（60 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CN 2–61（60 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AN 2–61（60 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CN 2–61（60 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AN 2–61（60 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CN 2–61（60 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AN 2–61（60 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CN 2–61（60 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AN 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CN 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BN 2–61（60 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DN 2–61（60 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AN 2–61（60 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CN 2–61（60 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 5I 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 5A 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS14 2–61（60 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS14 2–61（60 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1n 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2n 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BR 2–61（60 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain OA 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain KC 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 5I 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 5A 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain WA 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BD 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1n 1–61（61 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2n 1–61（61 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain WA 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AD 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain XA 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain CD 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain VA 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain YC 1–61（61 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS14 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS14 2–61（60 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

26 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS14Z_THET2
Isoform —
PDB entities 47
Chains and sequence ranges Author chain o； PDBConstruct 1–61； UniProt 1–61

30S ribosomal protein S15

OrganismNot specified

UniProt P62657

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain p； UniProt 1–89 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain O 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain p 1–89（89 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AO 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CO 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AO 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CO 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AO 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DO 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AO 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CO 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AO 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CO 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AO 2–89（88 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CO 2–89（88 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AO 2–89（88 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CO 2–89（88 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AO 2–89（88 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CO 2–89（88 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AO 2–89（88 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CO 2–89（88 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AO 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CO 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BO 2–89（88 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DO 2–89（88 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AO 2–89（88 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CO 2–89（88 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 6I 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 6A 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS15 2–89（88 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS15 2–89（88 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1o 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2o 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BS 2–89（88 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain PA 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain LC 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 6I 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 6A 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain XA 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CD 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1o 1–89（89 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2o 1–89（89 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain XA 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BD 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain YA 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain DD 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain WA 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ZC 1–89（89 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS15 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS15 2–89（88 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

26 other PDB entries and 49 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS15_THET2
Isoform —
PDB entities 48
Chains and sequence ranges Author chain p； PDBConstruct 1–89； UniProt 1–89

30S ribosomal protein S16

OrganismNot specified

UniProt P62238

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain q； UniProt 1–88 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain P 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain q 1–88（88 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AP 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CP 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AP 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CP 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AP 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DP 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AP 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CP 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AP 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CP 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AP 1–84（84 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CP 1–84（84 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AP 1–84（84 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CP 1–84（84 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AP 1–84（84 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CP 1–84（84 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AP 1–84（84 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CP 1–84（84 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AP 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CP 1–83（83 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BP 1–83（83 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DP 1–83（83 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AP 1–84（84 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CP 1–84（84 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 7I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 7A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 7I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 7A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 7I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 7A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 7I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 7A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 7I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 7A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS16 1–83（83 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS16 1–83（83 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1p 1–82（82 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2p 1–82（82 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BT 1–83（83 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain QA 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain MC 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 7I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 7A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain YA 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DD 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1p 1–88（88 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2p 1–88（88 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain YA 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CD 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain ZA 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain ED 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain XA 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AD 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AP 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CP 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS16 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS16 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 632 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 524 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 710 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2p 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 629 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279

36 other PDB entries and 69 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS16_THET2
Isoform —
PDB entities 49
Chains and sequence ranges Author chain q； PDBConstruct 1–88； UniProt 1–88

30S ribosomal protein S17

OrganismNot specified

UniProt P62658

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain r； UniProt 1–105 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1XMO Crystal Structure of mnm5U34t6A37-tRNALysUUU Complexed with AAG-mRNA in the Decoding Center Deposited 2004-10-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain Q 0–104（105 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 107 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, magnesium chloride, potassium chloride, ammonium chloride, MES-KOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.K
Resolution 3.25 Å R-free 0.284
1XMQ Crystal Structure of t6A37-ASLLysUUU AAA-mRNA Bound to the Decoding Center Deposited 2004-10-04 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain Q 0–104（105 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 107 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, magnesium chloride, potassium chloride, ammonium chloride, MES-KOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.K
Resolution 3.00 Å R-free 0.236
1XNQ Structure of an Inosine-Adenine Wobble Base Pair Complex in the Context of the Decoding Center Deposited 2004-10-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain Q 0–104（105 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 107 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, magnesium chloride, potassium chloride, ammonium chloride, MES-KOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.K
Resolution 3.05 Å R-free 0.270
1XNR Crystal Structure of an Inosine-Cytosine Wobble Base Pair in the Context of the Decoding Center Deposited 2004-10-05 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain Q 0–104（105 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 108 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, magnesium chloride, potassium chloride, ammonium chloride, MES-KOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.K
Resolution 3.10 Å R-free 0.273
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain r 1–105（105 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AQ 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CQ 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AQ 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CQ 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AQ 2–100（99 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DQ 2–100（99 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AQ 2–100（99 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CQ 2–100（99 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AQ 2–100（99 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CQ 2–100（99 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AQ 2–101（100 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CQ 2–101（100 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AQ 2–101（100 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CQ 2–101（100 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AQ 2–101（100 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CQ 2–101（100 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AQ 2–101（100 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CQ 2–101（100 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AQ 2–100（99 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CQ 2–100（99 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BQ 2–100（99 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DQ 2–100（99 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS17 2–100（99 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS17 2–100（99 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BU 2–105（104 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain RA 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain NC 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ZA 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ED 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ZA 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DD 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain AB 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain FD 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain YA 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BD 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS17 2–105（104 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS17 2–105（104 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

24 other PDB entries and 42 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS17_THET2
Isoform —
PDB entities 50
Chains and sequence ranges Author chain r； PDBConstruct 1–105； UniProt 1–105

30S ribosomal protein S18

OrganismNot specified

UniProt P62659

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain s； UniProt 1–88 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain R 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain QR 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 325 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.278
4L71 Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome Deposited 2013-06-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain XR 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 351 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；293 K；4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MgCl, pH 7.0, vapor diffusion, temperature 293K
Resolution 3.90 Å R-free 0.278
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain s 1–88（88 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V5L The structure of EF-Tu and aminoacyl-tRNA bound to the 70S ribosome with a GTP analog Deposited 2010-09-02 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 59-meric
Chain AR 1–88（88 aa）
Not recorded PAR PAROMOMYCIN × 1 ZN ZINC ION × 4 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.3；100 MM MES PH 6.3, 60-100 MM KCL, 50 MM SUCROSE, 1% GLYCEROL, AND 5.3% (W/V) PEG20K
Resolution 3.10 Å R-free 0.268
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AR 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CR 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AR 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CR 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AR 19–88（70 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DR 19–88（70 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AR 19–88（70 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CR 19–88（70 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AR 19–88（70 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CR 19–88（70 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AR 19–88（70 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CR 19–88（70 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AR 19–88（70 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CR 19–88（70 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AR 19–88（70 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CR 19–88（70 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AR 19–88（70 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CR 19–88（70 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AR 19–88（70 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CR 19–88（70 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BR 19–88（70 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DR 19–88（70 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AR 19–88（70 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CR 19–88（70 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 9I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 9A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 9I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 652 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WR6 Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 9A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 508 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 9I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 661 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WRA Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin. Deposited 2014-10-23 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 54-MERIC
Chain 9A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 497 PAR PAROMOMYCIN × 1 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.05 Å R-free 0.249
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 9I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 708 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WU1 Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site Deposited 2014-10-30 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain 9A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 673 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.20 Å R-free 0.238
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 9I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 699 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4WZD Complex of 70S ribosome with cognate tRNA-Tyr in the P-site Deposited 2014-11-19 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 9A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 646 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.193
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS18 19–88（70 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS18 19–88（70 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1r 20–87（68 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2r 20–87（68 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain SA 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain OC 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 9I 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain 9A 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AB 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain FD 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1r 1–88（88 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2r 1–88（88 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AB 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ED 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain BB 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain GD 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ZA 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CD 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain AR 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 218 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6C5L Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus) Deposited 2018-01-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 53 PDB declaration: 59-meric
Chain CR 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 256 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；292 K；5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME
Resolution 3.20 Å R-free 0.247
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 1r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1492 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFJ Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 56-meric
Chain 2r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1113 K POTASSIUM ION × 2 EZG N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.80 Å R-free 0.269
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1422 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFK Crystal structure of the Thermus thermophilus 70S ribosome in complex with D-histidyl-CAM and bound to protein Y (YfiA) at 2.7A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 940 K POTASSIUM ION × 1 EZP N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-D-histidinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.70 Å R-free 0.253
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 1r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 1474 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6CFL Crystal structure of the Thermus thermophilus 70S ribosome in complex with lysyl-CAM and bound to protein Y (YfiA) at 2.6A resolution Deposited 2018-02-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 53-meric
Chain 2r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 955 K POTASSIUM ION × 1 EZM N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-lysinamide × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 2.60 Å R-free 0.252
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS18 2–88（87 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS18 2–88（87 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 632 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9E Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 524 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.278
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 1r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 710 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279
6N9F Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic ACCA-DPhe and bound to mRNA and P-site tRNA at 3.7A resolution Deposited 2018-12-03 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain 2r 1–88（88 aa）
Not recorded MG MAGNESIUM ION × 629 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；292 K；0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME
Resolution 3.70 Å R-free 0.279

37 other PDB entries and 71 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS18_THET2
Isoform —
PDB entities 51
Chains and sequence ranges Author chain s； PDBConstruct 1–88； UniProt 1–88

30S ribosomal protein S19

OrganismNot specified

UniProt P62660

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain t； UniProt 1–93 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S20 × 1 （P62661） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain S 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain t 1–93（93 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AS 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CS 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AS 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CS 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS 4–81（78 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DS 4–81（78 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS 4–81（78 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CS 4–81（78 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS 4–81（78 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CS 4–81（78 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AS 4–82（79 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CS 4–82（79 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AS 4–82（79 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CS 4–82（79 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AS 4–82（79 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CS 4–82（79 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AS 4–82（79 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CS 4–82（79 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS 4–81（78 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CS 4–81（78 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BS 4–81（78 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DS 4–81（78 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain AS 4–82（79 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. Deposited 2014-07-02 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 61-meric
Chain CS 4–82（79 aa）
Not recorded GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.80 Å R-free 0.331
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain AI 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain AA 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS19 4–81（78 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS19 4–81（78 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 1s 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome Deposited 2015-04-20 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55MERIC
Chain 2s 2–84（83 aa）
Not recorded MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
Resolution 3.10 Å R-free 0.271
5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP Deposited 2015-07-23 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 52 PDB declaration: 55-meric
Chain BW 2–81（80 aa）
Not recorded NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 ELECTRON MICROSCOPY
cryo-EM buffer 5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM vitrification conditions Cryogen ETHANE；LIQUID ETHANE
Resolution 4.70 Å
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain TA 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain PC 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain AI 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 772 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5E81 Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair Deposited 2015-10-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 47 PDB declaration: 55-meric
Chain AA 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 631 SF4 IRON/SULFUR CLUSTER × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；PEG 20000, PEG 550mme, KSCN, Tris-OAc
Resolution 2.95 Å R-free 0.242
5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain QS 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 1425 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.04 Å R-free 0.279
5J3C Thermus thermophilus 70S termination complex containing E. coli RF1 Deposited 2016-03-30 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain XS 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 1011 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；5.2% PEG 20k, 10.4% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC
Resolution 3.04 Å R-free 0.279
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BB 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain GD 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1s 1–93（93 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2s 1–93（93 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BB 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain FD 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain CB 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain HD 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AB 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DD 1–93（93 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS19 2–93（92 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS19 2–93（92 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

27 other PDB entries and 51 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS19_THET2
Isoform —
PDB entities 52
Chains and sequence ranges Author chain t； PDBConstruct 1–93； UniProt 1–93

30S ribosomal protein S20

OrganismNot specified

UniProt P62661

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain u； UniProt 1–106 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein Thx × 1 （P62613） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2E5L A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction Deposited 2006-12-21 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain T 1–105（105 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.8；293 K；10mM HEPES-KOH, 10mM MgCl2, 60mM NH4Cl, 6mM beta-mercaptoethanol, 11-12% MPD, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 3.30 Å R-free 0.301
2F4V 30S ribosome + designer antibiotic Deposited 2005-11-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: 21-meric
Chain T 1–105（105 aa）
Not recorded MG MAGNESIUM ION × 101 K POTASSIUM ION × 12 D2C (2S,4S,4AR,5AS,6S,11R,11AS,12R,12AR)-7-CHLORO-4-(DIMETHYLAMINO)-6,10,11,12-TETRAHYDROXY-1,3-DIOXO-1,2,3,4,4A,5,5A,6,11,11A,12,12A-DODECAHYDROTETRACENE-2-CARBOXAMIDE × 1 AB9 (2R)-4-AMINO-N-{(1R,2S,3R,4R,5S)-5-AMINO-2-{2-[(2-AMINOETHYL)AMINO]ETHOXY}-4-[(2,6-DIAMINO-2,6-DIDEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-3-HYDROXYCYCLOHEXYL}-2-HYDROXYBUTANAMIDE × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 3.80 Å R-free 0.315
2VQE Modified uridines with C5-methylene substituents at the first position of the tRNA anticodon stabilize U-G wobble pairing during decoding Deposited 2008-03-13 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain T 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 206 K POTASSIUM ION × 62 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；SEE WIMBERLY ET AL., NATURE 2000, pH 6.5
Resolution 2.50 Å R-free 0.283
2VQF Modified uridines with C5-methylene substituents at the first position of the tRNA anticodon stabilize U-G wobble pairing during decoding Deposited 2008-03-14 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain T 1–106（106 aa）
Not recorded PAR PAROMOMYCIN × 1 MG MAGNESIUM ION × 214 K POTASSIUM ION × 73 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.5；SEE WIMBERLY ET AL.,NATURE, 2000, pH 6.5
Resolution 2.90 Å R-free 0.258
2ZM6 Crystal structure of the Thermus thermophilus 30S ribosomal subunit Deposited 2008-04-11 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 21-meric
Chain T 2–106（105 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.8；298 K；10mM HEPES-KOH, 10mM MgCl2, 60mM NH4Cl, 6mM beta-mercaptoethanol, 11-12% MPD, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 3.30 Å R-free 0.323
3T1H Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human anti-codon stem loop (HASL) of transfer RNA lysine 3 (tRNALys3) bound to an mRNA with an AAA-codon in the A-site and Paromomycin Deposited 2011-07-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain T 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 185 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 3.11 Å R-free 0.276
3T1Y Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human anti-codon stem loop (HASL) of transfer RNA Lysine 3 (TRNALYS3) bound to an mRNA with an AAG-codon in the A-site and paromomycin Deposited 2011-07-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 20 PDB declaration: 23-meric
Chain T 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 186 PAR PAROMOMYCIN × 1 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.80 Å R-free 0.268
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain T 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain u 1–106（106 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V4R Crystal structure of the whole ribosomal complex. Deposited 2005-09-30 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AT 0–105（106 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；277 K；PEG20k, MES buffer, magnesium acetate, potassium chloride, ammonium chloride, pH 6.7, VAPOR DIFFUSION, temperature 277K
Resolution 5.90 Å R-free 0.371
4V4S Crystal structure of the whole ribosomal complex. Deposited 2005-10-12 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AT 0–105（106 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；277 K；PEG20k, MES buffer, magnesium acetate, potassium chloride, ammonium chloride, pH 6.7, VAPOR DIFFUSION, temperature 277K
Resolution 6.76 Å R-free 0.356
4V4T Crystal structure of the whole ribosomal complex with a stop codon in the A-site. Deposited 2005-10-12 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain AT 0–105（106 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 6.7；277 K；PEG20k, MES buffer, magnesium acetate, potassium chloride, ammonium chloride, pH 6.7, VAPOR DIFFUSION, temperature 277K
Resolution 6.46 Å R-free 0.361
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AT 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CT 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AT 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CT 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AT 8–106（99 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DT 8–106（99 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AT 8–106（99 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CT 8–106（99 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AT 8–106（99 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CT 8–106（99 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AT 8–106（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CT 8–106（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
Resolution 3.86 Å R-free 0.317
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AT 8–106（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CT 8–106（99 aa）
Not recorded GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.309
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain AT 8–106（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-24 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 56 PDB declaration: 61-meric
Chain CT 8–106（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 3.50 Å R-free 0.328
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain AT 8–106（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. Deposited 2013-04-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 55 PDB declaration: 60-meric
Chain CT 8–106（99 aa）
Not recorded FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
Resolution 4.00 Å R-free 0.329
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AT 8–106（99 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CT 8–106（99 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BT 8–106（99 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DT 8–106（99 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AS20 8–106（99 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BS20 8–106（99 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain UA 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain QC 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CB 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain HD 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1t 1–106（106 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2t 1–106（106 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CB 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome Deposited 2017-09-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain GD 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain DB 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain ID 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BB 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ED 1–106（106 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AS20 2–106（105 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BS20 2–106（105 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

31 other PDB entries and 50 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name RS20_THET2
Isoform —
PDB entities 53
Chains and sequence ranges Author chain u； PDBConstruct 1–106； UniProt 1–106

30S ribosomal protein Thx

OrganismNot specified

UniProt P62613

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein–RNA Heteromer Protein × 48 RNA 6 PDB declaration: 54-meric（54） Consistent with all polymer counts Chain v； UniProt 1–27 Not recorded 23S LARGE SUBUNIT RIBOSOMAL RNA × 1 5S LARGE SUBUNIT RIBOSOMAL RNA × 1 50S ribosomal protein L1 × 1 （Q72GV9） 50S ribosomal protein L2 × 1 （Q72I07） 50S ribosomal protein L3 × 1 （Q72I04） 50S ribosomal protein L4 × 1 （Q72I05） 50S ribosomal protein L5 × 1 （Q72I16） 50S ribosomal protein L6 × 1 （Q72I19） 50S ribosomal protein L9 × 1 （Q72GV5） 50S ribosomal protein L13 × 1 （Q72IN1） 50S ribosomal protein L14 × 1 （Q72I14） 50S ribosomal protein L15 × 1 （Q72I23） 50S ribosomal protein L16 × 1 （Q72I11） 50S ribosomal protein L17 × 1 （Q72I33） 50S ribosomal protein L18 × 1 （Q72I20） 50S ribosomal protein L19 × 1 （Q72JU9） 50S ribosomal protein L20 × 1 （Q72L76） 50S ribosomal protein L21 × 1 （Q72HR2） 50S ribosomal protein L22 × 1 （Q72I09） 50S ribosomal protein L23 × 1 （Q72I06） 50S ribosomal protein L24 × 1 （Q72I15） 50S ribosomal protein L25 × 1 （Q72IA7） 50S ribosomal protein L27 × 1 （Q72HR3） LSU ribosomal protein L28P × 1 （Q72G84） 50S ribosomal protein L29 × 1 （Q72I12） LSU ribosomal protein L30P × 1 （Q72I22） 50S ribosomal protein L32 × 1 （P62652） 50S ribosomal protein L34 × 1 （P80340） 50S ribosomal protein L35 × 1 （Q72L77） 50S ribosomal protein L36 × 1 （Q72I28） 16S RNA × 1 P-site tRNAfMET × 1 E-site tRNA × 1 MRNA × 1 30S ribosomal protein S2 × 1 （P62662） 30S ribosomal protein S3 × 1 （P62663） 30S ribosomal protein S4 × 1 （P62664） 30S ribosomal protein S5 × 1 （P62665） 30S ribosomal protein S6 × 1 （P62666） 30S ribosomal protein S7 × 1 （P62667） 30S ribosomal protein S8 × 1 （P62668） 30S ribosomal protein S9 × 1 （P62669） 30S ribosomal protein S10 × 1 （P62653） 30S ribosomal protein S11 × 1 （P62654） 30S ribosomal protein S12 × 1 （P61941） 30S ribosomal protein S13 × 1 （P62655） 30S ribosomal protein S14 type Z × 1 （P62656） 30S ribosomal protein S15 × 1 （P62657） 30S ribosomal protein S16 × 1 （P62238） 30S ribosomal protein S17 × 1 （P62658） 30S ribosomal protein S18 × 1 （P62659） 30S ribosomal protein S19 × 1 （P62660） 30S ribosomal protein S20 × 1 （P62661） X-RAY DIFFRACTION X-ray crystallization conditions：pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K Resolution 3.83 Å R-free 0.351

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 4V4J Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
4KVB Thermus thermophilus HB27 30S ribosomal subunit lacking ribosomal protein S17 Deposited 2013-05-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 19 PDB declaration: eicosameric
Chain U 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 158 K POTASSIUM ION × 35 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions HANGING DROP；pH 6.5；277 K；MPD, pH 6.5, HANGING DROP, temperature 277K
Resolution 4.20 Å R-free 0.258
4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. Deposited 2007-02-15 Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain v 1–27（27 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.71 Å R-free 0.353
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AU 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 1260 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V63 Structural basis for translation termination on the 70S ribosome. Deposited 2008-05-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CU 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 1317 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 mM TRIS-OAc, 200 mM KSCN, 3.5-4.5 % (w/v) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.21 Å R-free 0.319
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain AU 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 1587 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V67 Crystal structure of a translation termination complex formed with release factor RF2. Deposited 2008-10-27 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CU 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 801 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K
Resolution 3.00 Å R-free 0.316
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AU 2–25（24 aa）
Not recorded MG MAGNESIUM ION × 250 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V7P Recognition of the amber stop codon by release factor RF1. Deposited 2010-04-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain DU 2–25（24 aa）
Not recorded MG MAGNESIUM ION × 160 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；295.5 K；crystals were grown by the sitting-drop vapor diffusion method dispensed robotically using 1-2 uL ribosome complexes mixed with 1-2 uL reservoir solution (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.4-4.8% PEG20,000, 2.5-11.5% PEG200) at 22.5 C, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
Resolution 3.62 Å R-free 0.291
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AU 2–25（24 aa）
Not recorded MG MAGNESIUM ION × 1169 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V83 Crystal structure of a complex containing domain 3 from the PSIV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CU 2–25（24 aa）
Not recorded MG MAGNESIUM ION × 1488 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.50 Å R-free 0.264
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain AU 2–25（24 aa）
Not recorded MG MAGNESIUM ION × 595 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V84 Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. Deposited 2010-12-13 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain CU 2–25（24 aa）
Not recorded MG MAGNESIUM ION × 599 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7；295 K；100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 3.6-5% PEG20,000, 6-14% MPD, vapor diffusion, temperature 295K
Resolution 3.40 Å R-free 0.266
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain AU 2–25（24 aa）
Not recorded MG MAGNESIUM ION × 1284 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9N Crystal structure of the 70S ribosome bound with the Q253P mutant of release factor RF2. Deposited 2013-04-26 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain CU 2–25（24 aa）
Not recorded MG MAGNESIUM ION × 1126 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain BU 2–25（24 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2050 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4V9Q Crystal Structure of Blasticidin S Bound to Thermus Thermophilus 70S Ribosome. Deposited 2013-06-12 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 54-meric
Chain DU 2–25（24 aa）
Not recorded BLS BLASTICIDIN S × 1 MG MAGNESIUM ION × 2313 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；Mix complex 1:1 with solution containing 4% PEG 20000, 8% MPD, 0.2M KSCN, 0.1M Tris-HCl , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Resolution 3.40 Å R-free 0.268
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 1F 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 618 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4WQ1 Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site. Deposited 2014-10-21 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 48 PDB declaration: 55-meric
Chain 1B 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 513 ZN ZINC ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；Tris-acetate, KSCN, PEG 550MME, PEG 20K
Resolution 3.10 Å R-free 0.235
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain ATHX 2–25（24 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
4XEJ IRES bound to bacterial Ribosome Deposited 2014-12-23 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 53-meric
Chain BTHX 2–25（24 aa）
Not recorded ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；295.5 K；Initial crystallization screening was performed around conditions previously reported (31, 48) by dispensing 0.2 + 0.2 mL sitting drops with a Phoenix robotic liquid handling system (Art Robbins) on 96-well plates. Once optimal crystallization conditions were determined, crystals were grown by the sitting-drop vapor-diffusion method using drops dispensed by the Phoenix with 1- to 2-mL ribosome complexes mixed with 1-2 mL of reservoir solution [100 mM Tris-OAc, pH 7.0, 200 mM potassium thiocyanate (KSCN), 3.6-5% PEG 20,000, 6-14% 2-methyl-2,4-pentanediol (MPD)] at 22.5C. Crystals emerged after 5-7 d and matured between 2-3 wk. Crystals were then subjected to cryoprotection by gradually replacing the mother liquor with cryoprotection buffer I (100 mM Tris-OAc, pH 7.0, 200 mM KSCN, 5% PEG 20,000, 25% MPD, 14% PEG 200, and 10 mM Mg(OAc)2). The crystals then were flash-frozen by plunging into liquid nitrogen.
Resolution 3.80 Å R-free 0.284
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain VA 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 1069 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5D8B Crystal structure of T. thermophilus ribosome containing a P-site wobble mismatch Deposited 2015-08-17 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 55-meric
Chain RC 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 1149 ZN ZINC ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 3-fold molar excess of E. coli release factor 1 and 650 uM blasticidin S added during complex formation to stabilize the complex
Resolution 3.63 Å R-free 0.287
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain DB 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon Deposited 2016-03-31 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain ID 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
Resolution 3.10 Å R-free 0.256
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 1u 1–27（27 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 Deposited 2017-03-22 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 49 PDB declaration: 52-meric
Chain 2u 1–27（27 aa）
Not recorded MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
Resolution 3.25 Å R-free 0.270
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain EB 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 51 PDB declaration: 57-meric
Chain JD 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.40 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain CB 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 826 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome Deposited 2017-11-20 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain FD 1–27（27 aa）
Not recorded MG MAGNESIUM ION × 692 ZN ZINC ION × 5 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
Resolution 3.55 Å
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein–RNA Heteromer；Protein × 50 PDB declaration: 55-meric
Chain ATHX 2–27（26 aa）
Not recorded MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321
6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State Deposited 2018-11-08 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein–RNA Heteromer；Protein × 50 PDB declaration: 56-meric
Chain BTHX 2–27（26 aa）
Not recorded MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 X-RAY DIFFRACTION
X-ray crystallization conditions LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.20 Å R-free 0.321

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UniProt name RSHX_THET2
Isoform —
PDB entities 54
Chains and sequence ranges Author chain v； PDBConstruct 1–27； UniProt 1–27

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 4v4j

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 4v4j
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2. Structure Basics 2. Structure Basics

Entry ID entry_id4v4j
Deposition date deposition_date2007-07-18
Structure title titleInteractions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome.
Keywords keywords70S ribosome, Shine-Dalgarno, RIBOSOME; RIBOSOME
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier83.59 Å
Radius of gyration Rg (electron density) rg_electron84.64 Å
Forward intensity I(0) i0154847000000.00
Molecular weight molecular_weight2185100.0 kDa
Excluded volume excluded_volume2250300 ų
Envelope volume envelope_volume4030000 ų
Hydration-shell volume shell_volume361050 ų
Envelope diameter envelope_diameter283.3 Å
Shell Rg shell_rg100.10 Å
Envelope Rg envelope_rg83.59 Å
Shape Rg shape_rg84.71 Å
Total Rg total_rg84.57 Å
Total atoms total_atoms147125
Residues n_residues10478
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax293.7 Å
Rg (real space) rg_real85.77 Å
Rg uncertainty (real space) rg_real_error1.38 Å
I(0) (real space) i0_real1.5350e+11
I(0) uncertainty (real space) i0_real_error3.0920e+09
Rg (reciprocal space) rg_reciprocal84.98 Å
I(0) (reciprocal space) i0_reciprocal155500000000.0000
Solution quality estimate total_estimate0.9051
Solution quality rating solution_quality EXCELLENT a EXCELLENT solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary108.2 Å
Skewness Skewness skewness0.378
Kurtosis Kurtosis kurtosis0.068
Angular range angular_range— – 0.0950 Å−1
Current regularization parameter α current_alpha1.1850
Highest regularization parameter α highest_alpha10630000000.0000
Real-space data points n_real_points20
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.763; Stabil: 0.912; Sysdev: 1.000; Positv: 1.000; Valcen: 0.959; Smooth: 0.796
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (54)

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8. Citations (1)

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9. Files and Curves (10)