当前蛋白身份:P63279 重新检索
本组结构的主要差异维度
构建体不同 突变/修饰不同 组装状态不同 配体/离子不同 实验方法不同 实验环境不同 结构质量指标不同

差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。

相关结构差异明细

一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。

PDB 条目 Assembly / 聚集状态 构建体 突变与修饰 配体、离子与非聚合物 实验方法 实验环境 结构质量
1A3S HUMAN UBC9 提交 1998-01-23 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 pH 7;pH 7.
分辨率 2.80 Å R-free 0.260
1KPS Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1 提交 2002-01-02 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
未记录 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 7;291 K;2.0M ammonium phosphate, 0.1M hepes, 10mM CuCl2, 5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
分辨率 2.50 Å R-free 0.300
1KPS Structural Basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin conjugating enzyme Ubc9 and RanGAP1 提交 2002-01-02 Assembly 2 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 C 1–158(158 aa)
未记录 SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 7;291 K;2.0M ammonium phosphate, 0.1M hepes, 10mM CuCl2, 5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
分辨率 2.50 Å R-free 0.300
1Z5S Crystal structure of a complex between UBC9, SUMO-1, RANGAP1 and NUP358/RANBP2 提交 2005-03-19 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 4 PDB 声明:tetrameric(4) 与蛋白数一致
链 A 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 5;291 K;18% PEG4000 (w/v), 0.1 M sodium citrate, 0.2 M ammonium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
分辨率 3.01 Å R-free 0.290
2GRN Crystal Structure of human RanGAP1-Ubc9 提交 2006-04-24 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 5.5;291 K;1M lithium sulfate, 0.5M ammonium sulfate, 50mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
分辨率 1.80 Å R-free 0.218
2GRO Crystal Structure of human RanGAP1-Ubc9-N85Q 提交 2006-04-24 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
突变:N85Q 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 5.5;291 K;1M lithium sulfate, 0.5M ammonium sulfate, 50mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
分辨率 1.70 Å R-free 0.202
2GRP Crystal Structure of human RanGAP1-Ubc9-Y87A 提交 2006-04-24 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
突变:Y87A 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 5.5;291 K;1M lithium sulfate, 0.5M ammonium sulfate, 50mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
分辨率 2.05 Å R-free 0.231
2GRQ Crystal Structure of human RanGAP1-Ubc9-D127A 提交 2006-04-24 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
突变:D127A 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 5.5;291 K;1M lithium sulfate, 0.5M ammonium sulfate, 50mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
分辨率 1.70 Å R-free 0.205
2GRR Crystal Structure of human RanGAP1-Ubc9-D127S 提交 2006-04-24 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
突变:D127S 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 5.5;291 K;1M lithium sulfate, 0.5M ammonium sulfate, 50mM sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
分辨率 1.30 Å R-free 0.222
2O25 Ubiquitin-Conjugating Enzyme E2-25 kDa Complexed With SUMO-1-Conjugating Enzyme UBC9 提交 2006-11-29 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 D 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 6;298 K;The protein complex (1:1) was dissolved at 26 mg/ml in 20 mM Tris-HCl, pH 8.0, 5% glycerol, 2 mM DTT. Hanging drops (2 microL + 2 microL), room temperature, well solution: 16% PEG MME 5000, 0.1 M bis-Tris, pH 6.0, 1 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
分辨率 2.60 Å R-free 0.291
2O25 Ubiquitin-Conjugating Enzyme E2-25 kDa Complexed With SUMO-1-Conjugating Enzyme UBC9 提交 2006-11-29 Assembly 2 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 C 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 6;298 K;The protein complex (1:1) was dissolved at 26 mg/ml in 20 mM Tris-HCl, pH 8.0, 5% glycerol, 2 mM DTT. Hanging drops (2 microL + 2 microL), room temperature, well solution: 16% PEG MME 5000, 0.1 M bis-Tris, pH 6.0, 1 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
分辨率 2.60 Å R-free 0.291
2PE6 Non-covalent complex between human SUMO-1 and human Ubc9 提交 2007-04-02 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 pH 8.5;291 K;100 mM Tris-HCl, pH 8.5, 25% PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.50
分辨率 2.40 Å R-free 0.250
2PX9 The intrinsic affinity between E2 and the Cys domain of E1 in Ubiquitin-like modifications 提交 2007-05-14 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 B 1–158(158 aa)
未记录 未记录非水小分子 SOLUTION NMR
NMR测量条件 pH 7.2;298 K;离子强度(mmCIF原始值)50mM Phosphate;压力 1
NMR样品组成 0.15-0.6mM E1 Cys domain, 0.15-0.7mM Ubc9, 50mM Phosphate buffer | 50mM Phosphate buffer
分辨率未提供
2XWU CRYSTAL STRUCTURE OF IMPORTIN 13 - UBC9 COMPLEX 提交 2010-11-05 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 pH 6.5;50 MM MES PH 6.5 25% PEG 300
分辨率 2.80 Å R-free 0.268
3A4S The crystal structure of the SLD2:Ubc9 complex 提交 2009-07-14 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 6;277 K;25% polyethylene glycol 1500, 0.1M SPG buffer : SPG buffer was prepared by mixing succinic acid, sodium dihydrogen phosphate, and glycine in the molar ratios 2:7:7, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
分辨率 2.70 Å R-free 0.269
3A4S The crystal structure of the SLD2:Ubc9 complex 提交 2009-07-14 Assembly 2 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 B 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 6;277 K;25% polyethylene glycol 1500, 0.1M SPG buffer : SPG buffer was prepared by mixing succinic acid, sodium dihydrogen phosphate, and glycine in the molar ratios 2:7:7, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
分辨率 2.70 Å R-free 0.269
3UIN Complex between human RanGAP1-SUMO2, UBC9 and the IR1 domain from RanBP2 提交 2011-11-05 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 4 PDB 声明:tetrameric(4) 与蛋白数一致
链 A 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 7.5;279 K;100 mM HEPES pH 7.5, 400 mM ammonium citrate, 2% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 279K
分辨率 2.60 Å R-free 0.264
3UIO Complex between human RanGAP1-SUMO2, UBC9 and the IR1 domain from RanBP2 containing IR2 Motif II 提交 2011-11-05 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 4 PDB 声明:tetrameric(4) 与蛋白数一致
链 A 1–158(158 aa)
非标准单体:是(mmCIF未提供具体位点) 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 6;279 K;14% PEG4000, 100 mM sodium citrate pH 6.0, 200 mM ammonium acetate, vapor diffusion, hanging drop, temperature 279K
分辨率 2.60 Å R-free 0.251
3UIP Complex between human RanGAP1-SUMO1, UBC9 and the IR1 domain from RanBP2 containing IR2 Motif II 提交 2011-11-05 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 4 PDB 声明:tetrameric(4) 与蛋白数一致
链 A 1–158(158 aa)
非标准单体:是(mmCIF未提供具体位点) 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 7.5;279 K;22% PEG4000, 100 mM HEPES pH 7.5, 400 mM ammonium citrate, 2% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 279K
分辨率 2.29 Å R-free 0.232
4W5V Crystal structure of Human SUMO E2-conjugating enzyme (Ubc9) in complex with E1-activating enzyme (Uba2) ubiquitin fold domain (Ufd) 提交 2014-08-19 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
未记录 FMT FORMIC ACID × 1 GOL GLYCEROL × 1 K POTASSIUM ION × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;pH 8;293 K;100 mM Tris, pH 8.0, 20% PEG MME 550, 200 mM potassium formate, 20% glycerol; seeded
分辨率 2.50 Å R-free 0.283
4Y1L Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation 提交 2015-02-08 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 3 PDB 声明:trimeric(3) 与蛋白数一致
链 A 1–158(158 aa)
链 B 1–158(158 aa)
未记录 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION;pH 8;293 K;10% (w/v) PEG8000, 100 mM HEPES, pH 8.0, 8% ethylene glycol
分辨率 2.70 Å R-free 0.278
5D2M Complex between human SUMO2-RANGAP1, UBC9 and ZNF451 提交 2015-08-05 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 5 PDB 声明:pentameric(5) 与蛋白数一致
链 A 1–158(158 aa)
突变:K14R EDO 1,2-ETHANEDIOL × 3 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 7.5;291 K;6 % (w/v) PEG 8000, 0.2 M ammonium citrate and 0.1 M HEPES pH 7.5
分辨率 2.40 Å R-free 0.234
5D2M Complex between human SUMO2-RANGAP1, UBC9 and ZNF451 提交 2015-08-05 Assembly 2 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 D 1–158(158 aa)
突变:K14R EDO 1,2-ETHANEDIOL × 2 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 7.5;291 K;6 % (w/v) PEG 8000, 0.2 M ammonium citrate and 0.1 M HEPES pH 7.5
分辨率 2.40 Å R-free 0.234
5F6D Crystal structure of Ubc9 (K48A/K49A/E54A) complexed with Fragment 6 提交 2015-12-05 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 2–158(157 aa)
突变:K48A,K49A,E54A 5VJ 6~{H}-benzo[c][1,2]benzothiazine 5,5-dioxide × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION;pH 8.5;294 K;0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol 8000
分辨率 1.55 Å R-free 0.196
5F6E Crystal Structure of human Ubc9 (K48A/K49A/E54A) 提交 2015-12-05 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 2–158(157 aa)
突变:K48A,K49A,E54A EDO 1,2-ETHANEDIOL × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 8.5;294 K;0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol
分辨率 1.12 Å R-free 0.170
5F6U Crystal Structure of Ubc9 (K48A/K49A/E54A) complexed with Fragment 8 (JSS190B146) 提交 2015-12-07 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 2–158(157 aa)
突变:K48A,K49A,K54A 5VK ethyl 3-[4-(2-hydroxyphenyl)-3-oxidanyl-phenyl]propanoate × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 8.5;294 K;0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol
分辨率 1.55 Å R-free 0.203
5F6V Crystal structure of Ubc9 (K48/K49A/E54A) complexed with Fragment 1 (biphenol from fragment cocktail screen) 提交 2015-12-07 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 2–158(157 aa)
突变:K48A,K49A,E54A 5VL 2-(2-hydroxyphenyl)phenol × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 8.5;294 K;0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol 8000
分辨率 1.49 Å R-free 0.184
5F6W Crystal structure of Ubc9 (K48/K49A/E54A) complexed with Fragment 1 (biphenol) 提交 2015-12-07 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 2–158(157 aa)
突变:K48A,K49A,E54A 5VL 2-(2-hydroxyphenyl)phenol × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;294 K;0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol
分辨率 1.70 Å R-free 0.200
5F6X Crystal structure of Ubc9 (K48/K49A/E54A) complexed with Fragment 2 (mercaptobenzoxazole from cocktail screen) 提交 2015-12-07 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 2–158(157 aa)
突变:K48A,K49A,E54A 5VM 5-chloranyl-3~{H}-1,3-benzoxazole-2-thione × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 8.5;294 K;0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol 8000
分辨率 1.56 Å R-free 0.193
5F6Y Crystal structure of Ubc9 (K48/K49A/E54A) complexed with Fragment 2 (mercaptobenzoxazole) 提交 2015-12-07 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 A 2–158(157 aa)
突变:K48A,K49A,E54A 5VM 5-chloranyl-3~{H}-1,3-benzoxazole-2-thione × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 8.5;294 K;0.1M Tris pH 8.5, 8% (w/v) polyethylene glycol 8000
分辨率 1.14 Å R-free 0.178
5FQ2 Crystal structure of human SUMO E1 UFD domain in complex with Ubc9 in a P422 space group. 提交 2015-12-04 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 1–158(158 aa)
非标准单体:是(mmCIF未提供具体位点) 未记录非水小分子 X-RAY DIFFRACTION mmCIF 未提供已读取条件 分辨率 2.20 Å R-free 0.249
6SYF Human Ubc9 with covalent isopeptide ligand 提交 2019-09-27 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 3 PDB 声明:trimeric(3) 与蛋白数一致
链 A 2–158(157 aa)
突变:K48A, K49A, E54A, C138A 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;pH 5;293.15 K;0.1 M trisodium citrate pH 5.0, 30% PEG8000
分辨率 1.90 Å R-free 0.263
6SYF Human Ubc9 with covalent isopeptide ligand 提交 2019-09-27 Assembly 2 蛋白异源复合物 异源复合物;蛋白 × 3 PDB 声明:trimeric(3) 与蛋白数一致
链 B 2–158(157 aa)
突变:K48A, K49A, E54A, C138A 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;pH 5;293.15 K;0.1 M trisodium citrate pH 5.0, 30% PEG8000
分辨率 1.90 Å R-free 0.263
6SYF Human Ubc9 with covalent isopeptide ligand 提交 2019-09-27 Assembly 3 蛋白异源复合物 异源复合物;蛋白 × 3 PDB 声明:trimeric(3) 与蛋白数一致
链 C 2–158(157 aa)
突变:K48A, K49A, E54A, C138A 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;pH 5;293.15 K;0.1 M trisodium citrate pH 5.0, 30% PEG8000
分辨率 1.90 Å R-free 0.263
6SYF Human Ubc9 with covalent isopeptide ligand 提交 2019-09-27 Assembly 4 蛋白异源复合物 异源复合物;蛋白 × 3 PDB 声明:trimeric(3) 与蛋白数一致
链 D 2–158(157 aa)
突变:K48A, K49A, E54A, C138A 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;pH 5;293.15 K;0.1 M trisodium citrate pH 5.0, 30% PEG8000
分辨率 1.90 Å R-free 0.263
8ODR Mimetic of UBC9-SUMO1 提交 2023-03-09 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致
链 A 2–158(157 aa)
突变:K14R, A129K SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP;pH 8.5;293 K;LiSO4, Tris pH8.5, PEG 4000
分辨率 2.85 Å R-free 0.258
9B62 Human RANBP2/RAN(GTP)/RANGAP1-SUMO1/UBC9/CRM1/RAN(GTP) - composite map and model 提交 2024-03-23 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 7 PDB 声明:heptameric(7) 与蛋白数一致
链 C 1–158(158 aa)
未记录 GTP GUANOSINE-5'-TRIPHOSPHATE × 2 MG MAGNESIUM ION × 2 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 8;20 mM Tris-Cl pH 8.0, 50 mM NaCl, 0.1 mM TCEP supplemented with 0.02% (v/v) IGEPAL CA-630
cryo-EM玻璃化条件 冷冻剂 ETHANE;30 s wait time, blot for 2.5 s before plunging
分辨率 2.90 Å
9DQB Cryo-EM structure of a double-loaded SUMO E1-E2-SUMO1 complex. 提交 2024-09-23 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 5 PDB 声明:pentameric(5) 与蛋白数一致
链 C 1–158(158 aa)
突变:F22A,G23Q,V25S,A129K,C138S,K153R AMP ADENOSINE MONOPHOSPHATE × 1 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 3.60 Å
9DRJ Cryo-EM structure of a SUMO E1-E2-SUMO1 complex. 提交 2024-09-25 Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 4 PDB 声明:tetrameric(4) 与蛋白数一致
链 C 1–158(158 aa)
突变:C138S ZN ZINC ION × 1 AMP ADENOSINE MONOPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM缓冲液 pH 7.5
cryo-EM玻璃化条件 冷冻剂 ETHANE
分辨率 2.70 Å
9GLR Crystal Structure of Human UBC9 C93E 提交 2024-08-28 Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致
链 AAA 1–158(158 aa)
突变:C93E 未记录非水小分子 X-RAY DIFFRACTION
X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP;pH 5;293 K;2% tacsimate, pH 5.0, 0.1 M sodium citrate, 16% PEG 3350
分辨率 1.72 Å R-free 0.227