Current Protein Identity：P84022
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1MHD CRYSTAL STRUCTURE OF A SMAD MH1 DOMAIN BOUND TO DNA Deposited 1998-08-18 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
1–132（132 aa）
Fragment：MH1 DOMAIN, RESIDUES 1 - 144
Chain B
1–132（132 aa）
Fragment：MH1 DOMAIN, RESIDUES 1 - 144
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 5.6；pH 5.6
|
Resolution 2.80 Å R-free 0.288 |
| 1MJS MH2 domain of transcriptional factor SMAD3 Deposited 2002-08-28 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
229–425（197 aa）
Fragment：MH2 domain, residues 229-425
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 1.91 Å R-free 0.199 |
| 1MK2 SMAD3 SBD complex Deposited 2002-08-28 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
220–425（206 aa）
Fragment：MH2 domain, residues 220-425
|
Not recorded | ACY ACETIC ACID × 8 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.74 Å R-free 0.271 |
| 1MK2 SMAD3 SBD complex Deposited 2002-08-28 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
220–425（206 aa）
Fragment：MH2 domain, residues 220-425
|
Not recorded | ACY ACETIC ACID × 4 | X-RAY DIFFRACTION | mmCIF provides none of the parsed conditions | Resolution 2.74 Å R-free 0.271 |
| 1OZJ Crystal structure of Smad3-MH1 bound to DNA at 2.4 A resolution Deposited 2003-04-09 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
1–144（144 aa）
Fragment：DWA DOMAIN
Chain B
1–144（144 aa）
Fragment：DWA DOMAIN
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.5；293 K；citrate, PEG2000, ammonium acetate, spermine, magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.40 Å R-free 0.270 |
| 1U7F Crystal Structure of the phosphorylated Smad3/Smad4 heterotrimeric complex Deposited 2004-08-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
228–424（197 aa）
Fragment：MH2 and Linker domains
Chain C
228–424（197 aa）
Fragment：MH2 and Linker domains
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；50 mM Tris-HCl, 0-15 mM magnesium chloride, 5-15% ethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 2.60 Å R-free 0.247 |
| 2LAJ Third WW domain of human Nedd4L in complex with doubly phosphorylated human smad3 derived peptide Deposited 2011-03-16 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
202–211（10 aa）
Fragment：sequence database residues 202-211
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；285 K；Ionic strength (raw mmCIF value) 0.420；Pressure ambient
NMR sample composition
1 mM NEDD4LWW3, 3 mM SMAD3, 20 mM sodium phosphate, 100 mM sodium chloride, 2 mM sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 15N] NEDD4LWW3, 3 mM SMAD3, 20 mM sodium phosphate, 100 mM sodium chloride, 2 mM sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] NEDD4LWW3, 3 mM SMAD3, 20 mM sodium phosphate, 100 mM sodium chloride, 2 mM sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2LB2 Structure of the second domain of human Nedd4L in complex with a phosphorylated pTPY motif derived from human Smad3 Deposited 2011-03-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
178–189（12 aa）
Fragment：residues 178-189
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7；285 K；Ionic strength (raw mmCIF value) 0.420；Pressure ambient
NMR sample composition
1 mM NEDD4LWW3, 3 mM SMAD3, 20 mM sodium phosphate, 100 mM sodium chloride, 2 mM sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 15N] NEDD4LWW3, 3 mM SMAD3, 20 mM sodium phosphate, 100 mM sodium chloride, 2 mM sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
1 mM [U-100% 13C; U-100% 15N] NEDD4LWW3, 3 mM SMAD3, 20 mM sodium phosphate, 100 mM sodium chloride, 2 mM sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 5OD6 Crystal structure of Smad3-MH1 bound to the GGCGC site. Deposited 2017-07-04 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
11–135（125 aa）
Chain B
11–135（125 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.2 M lithium acetate, 20% PEG 3350
|
Resolution 2.00 Å R-free 0.235 |
| 5ODG Crystal structure of Smad3-MH1 bound to the GGCT site. Deposited 2017-07-05 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
11–135（125 aa）
Fragment：MH1 domain, UNP residues 11-135
Chain B
11–135（125 aa）
Fragment：MH1 domain, UNP residues 11-135
|
Not recorded | ZN ZINC ION × 2 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.02 M sodium potassium phosphate, 0.1 M BisTris propane pH 6.5, 20% PEG 3350
|
Resolution 2.12 Å R-free 0.242 |
| 5ODG Crystal structure of Smad3-MH1 bound to the GGCT site. Deposited 2017-07-05 | Assembly 2 Protein–DNA Homooligomer；Protein × 2 PDB declaration: tetrameric（4） Consistent with all polymers |
Chain A
11–135（125 aa）
Fragment：MH1 domain, UNP residues 11-135
Chain B
11–135（125 aa）
Fragment：MH1 domain, UNP residues 11-135
|
Not recorded | ZN ZINC ION × 2 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.02 M sodium potassium phosphate, 0.1 M BisTris propane pH 6.5, 20% PEG 3350
|
Resolution 2.12 Å R-free 0.242 |
| 5XOC Crystal structure of human Smad3-FoxH1 complex Deposited 2017-05-27 | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric（6） Consistent with protein count |
Chain A
220–416（197 aa）
Fragment：UNP residues 220-416
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.4；293 K；0.1 M citrate pH 5.4, 0.8% ethylene imine polymer and 0.5 M NaCl
|
Resolution 2.40 Å R-free 0.235 |
| 6ZMN Crystal structure of the Smad3-Smad5 MH1 domain chimera bound to the GGCGC site Deposited 2020-07-03 | Assembly 1 Protein–DNA Homooligomer；Protein × 2 PDB declaration: hexameric（6） Consistent with all polymers |
Chain A
10–136（127 aa）
Chain B
10–136（127 aa）
|
Not recorded | ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 1 ZN ZINC ION × 2 PGE TRIETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；20% PEG 3350, 0.2 M sodium acetate
|
Resolution 2.33 Å R-free 0.252 |