Current Protein Identity:Q00422
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AWC MOUSE GABP ALPHA/BETA DOMAIN BOUND TO DNA Deposited 1997-10-01 | Assembly 1 Protein–DNA Heteromer;Protein × 2 PDB declaration: tetrameric(4) Consistent with all polymers |
Chain A
320–429(110 aa)
Fragment:ETS DOMAIN PLUS 30 C-TERMINAL RESIDUES
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 9;100 MM BIS-TRIS PROPANE PH 9, 12 % PEG 1000, 5 MM COBALTIC HEXAMINE CHLORIDE, 1 MM DTT, 20 MM TRIS, 1 MM EDTA, 0.001 % SODIUM AZIDE, pH 9.0
|
Resolution 2.15 Å R-free 0.282 |
| 1SXD Solution Structure of the Pointed (PNT) Domain from mGABPa Deposited 2004-03-30 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
168–254(87 aa)
Fragment:PNT domain
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7.2;303 K;Ionic strength (raw mmCIF value) 40 mM;Pressure ambient
NMR measurement conditions
pH 7.2;303 K;Ionic strength (raw mmCIF value) 40 mM;Pressure ambient
NMR measurement conditions
pH 7.2;303 K;Ionic strength (raw mmCIF value) 40 mM;Pressure ambient
NMR sample composition
1mM GABPa U-15N,13C, 20mM phosphate buffer pH 7.2, 20mM NaCl, 2mM DTT, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
1mM GABPa 100%15N,10%13C, 20mM phosphate buffer pH 7.2 20mM NaCl, 2mM DTT, 90% H2O, 10% D2O | 90% H2O/10% D2O
NMR sample composition
2mM GABPa 100%15N, 20mM phosphate buffer pH 7.2 20mM NaCl, 2mM DTT, 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2JUO GABPa OST domain Deposited 2007-08-31 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
35–121(87 aa)
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR |
NMR measurement conditions
pH 7;298.1 K
NMR sample composition
20 mM sodium phosphate, 20 mM sodium chloride, 1 mM [U-100% 13C; U-100% 15N] GABPa OST, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
20 mM sodium phosphate, 50 mM sodium chloride, 1 mM [U-100% 15N] GABPa OST, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
20 mM sodium phosphate, 50 mM sodium chloride, 1 mM [U-10% 13C; U-100% 15N] GABPa OST, 2 mM DTT, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |