SAXSdb — 1awc

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1. Structure Basics

entry_id	1awc
deposition_date	1997-10-01
title	MOUSE GABP ALPHA/BETA DOMAIN BOUND TO DNA
keywords	;COMPLEX (TRANSCRIPTION REGULATION-DNA), DNA-BINDING, NUCLEAR PROTEIN, ETS DOMAIN, ANKYRIN REPEATS, TRANSCRIPTION FACTOR, TRANSCRIPTION-DNA COMPLEX ;; TRANSCRIPTION/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	25.04 Å
rg_electron	23.73 Å
i0	44658400.00
molecular_weight	42584.0 kDa
excluded_volume	49214 Å³
envelope_volume	63132 Å³
shell_volume	22886 Å³
envelope_diameter	82.0 Å
shell_rg	29.91 Å
envelope_rg	23.77 Å
shape_rg	23.66 Å
total_rg	24.52 Å
total_atoms	2920
n_residues	300
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	78.9 Å
rg_real	25.00 Å
rg_real_error	0.49 Å
i0_real	4.4660e+07
i0_real_error	6.1960e+05
rg_reciprocal	25.02 Å
i0_reciprocal	44660000.0000
total_estimate	0.9127
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	30.1 Å
skewness	0.213
kurtosis	-0.570
angular_range	— – 0.3150 Å⁻¹
current_alpha	0.0000
highest_alpha	3115000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.965; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.973; Smooth: 0.991

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4. Crystallography & Experiment

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5. Entities & Polymer Info (5)

Entity 1 polymer

description	;DNA (5'-D(APAP(BRU)PGPAPCPCPGPGPAPAPGPTPAP(CBR)PAPCP(CBR)PGPGP*A)-3') ;
formula_weight	6712.9 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	D
atom_count	2966

AAUGACCGGAAGTACACCGGA

Entity 2 polymer

description	;DNA (5'-D(TPTPCPCPGPGP(BRU)PGP(BRU)PAPCPTPTPCPCPGPGPTPCPAP*T)-3') ;
formula_weight	6525.9 kDa
src_method	syn
entity_count	1

Polymer

type	polydeoxyribonucleotide
strand_id	E

TTCCGGUGUACTTCCGGTCAT

Entity 3 polymer

description	PROTEIN (GA BINDING PROTEIN ALPHA)
formula_weight	13065.2 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

IQLWQFLLELLTDKDARDCISWVGDEGEFKLNQPELVAQKWGQRKNKPTMNYEKLSRALRYYYDGDMICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVIECEQKKLARM

Source

organism	Mus musculus
common_name	house mouse
ncbi_taxonomy_id	10090
host_organism	Escherichia coli BL21(DE3)

Entity 4 polymer

description	PROTEIN (GA BINDING PROTEIN BETA 1)
formula_weight	16834.0 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

DLGKKLLEAARAGQDDEVRILMANGAPFTTDWLGTSPLHLAAQYGHFSTTEVLLRAGVSRDARTKVDRTPLHMAASEGHANIVEVLLKHGADVNAKDMLKMTALHWATEHNHQEVVELLIKYGADVHTQSKFCKTAFDISIDNGNEDLAEILQ

Source

organism	Mus musculus
common_name	house mouse
ncbi_taxonomy_id	10090
host_organism	Escherichia coli BL21(DE3)

Entity 5 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	46

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6. Citations (1)

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length_a	201.600 Å
length_b	34.550 Å
length_c	59.260 Å
angle_alpha	90.00 °
angle_beta	99.83 °
angle_gamma	90.00 °
z_pdb	4

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	2.150 Å
resolution_low	6.000 Å
r_factor	—
r_work	0.2110
r_free	0.2820

fit	/app/data/saxs_computation/aw/1awc/1awc.fit
pddf	/app/data/pr_computation/aw/1awc/1awc.pr.out
profile	/app/data/saxs_computation/aw/1awc/1awc.dat

MOUSE GABP ALPHA/BETA DOMAIN BOUND TO DNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (5)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 polymer

Polymer

Source

Entity 5 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)