Current Protein Identity:Q07381
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 5IW7 Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor Deposited 2016-03-22 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
46–409(364 aa)
Chain A
477–788(312 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6;291 K;8-14% w/v PEG 3350
0.18-0.30 M sodium malonate pH 6.0
|
Resolution 3.60 Å R-free 0.295 |
| 5IW7 Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor Deposited 2016-03-22 | Assembly 2 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
46–409(364 aa)
Chain B
477–788(312 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6;291 K;8-14% w/v PEG 3350
0.18-0.30 M sodium malonate pH 6.0
|
Resolution 3.60 Å R-free 0.295 |
| 5IW7 Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor Deposited 2016-03-22 | Assembly 3 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain C
46–409(364 aa)
Chain C
477–788(312 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6;291 K;8-14% w/v PEG 3350
0.18-0.30 M sodium malonate pH 6.0
|
Resolution 3.60 Å R-free 0.295 |
| 5IW7 Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor Deposited 2016-03-22 | Assembly 4 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain D
46–409(364 aa)
Chain D
477–788(312 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6;291 K;8-14% w/v PEG 3350
0.18-0.30 M sodium malonate pH 6.0
|
Resolution 3.60 Å R-free 0.295 |
| 5WWN Crystal structure of Tsr1 Deposited 2017-01-03 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
80–788(709 aa)
Fragment:UNP residues 80-788
|
Not recorded | SO4 SULFATE ION × 10 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;293 K;0.1M sodium chloride, 0.08M BIS-TRIS pH 5.5, 0.02M BIS-TRIS pH 6.5, 1.6M ammonium sulfate
|
Resolution 2.81 Å R-free 0.274 |
| 6EML Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae Deposited 2017-10-02 | Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 31-meric(31) Consistent with protein count |
Chain t
1–788(788 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 6FAI Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit Deposited 2017-12-15 | Assembly 1 Protein–RNA Heteromer;Protein × 35 PDB declaration: 36-meric(36) Consistent with all polymers |
Chain k
1–788(788 aa)
|
Not recorded | ZN ZINC ION × 2 MG MAGNESIUM ION × 46 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE-PROPANE
|
Resolution 3.40 Å |
| 6RBD State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles Deposited 2019-04-10 | Assembly 1 Protein–RNA Heteromer;Protein × 34 PDB declaration: 35-meric(35) Consistent with all polymers |
Chain k
1–788(788 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE;blot 1.7-1.9 seconds before plunging
|
Resolution 3.47 Å |
| 6WDR Subunit joining exposes nascent pre-40S rRNA for processing and quality control Deposited 2020-04-01 | Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers |
Chain k
1–788(788 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 6.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 6Y7C Early cytoplasmic yeast pre-40S particle (purified with Tsr1 as bait) Deposited 2020-02-28 | Assembly 1 Protein–RNA Heteromer;Protein × 33 PDB declaration: 34-meric(34) Consistent with all polymers |
Chain t
1–788(788 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 7WTP Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-2 (with Rps2) Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer;Protein × 18 PDB declaration: nonadecameric(19) Consistent with all polymers |
Chain CC
1–788(788 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 7WTQ Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-2 (without Rps2) Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer;Protein × 17 PDB declaration: octadecameric(18) Consistent with all polymers |
Chain CC
1–788(788 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å |
| 7WTR Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-3 Deposited 2022-02-05 | Assembly 1 Protein–RNA Heteromer;Protein × 18 PDB declaration: nonadecameric(19) Consistent with all polymers |
Chain CC
1–788(788 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 8C00 Enp1TAP-S21_A population of yeast small ribosomal subunit precursors depleted of rpS21/eS21 Deposited 2022-12-15 | Assembly 1 Protein–RNA Heteromer;Protein × 25 PDB declaration: 26-meric(26) Consistent with all polymers |
Chain t
1–788(788 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 8C01 Enp1TAP_A population of yeast small ribosomal subunit precursors Deposited 2022-12-15 | Assembly 1 Protein–RNA Heteromer;Protein × 30 PDB declaration: 31-meric(31) Consistent with all polymers |
Chain t
1–788(788 aa)
|
Not recorded | ZN ZINC ION × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |