7wtq

Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-2 (without Rps2)

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

40S ribosomal protein S1-A

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS3A1_YEAST
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain SB； PDBConstruct 1–255； UniProt 1–255

40S ribosomal protein S4-A

OrganismNot specified

UniProt P0CX35

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS4A_YEAST
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain SE； PDBConstruct 1–261； UniProt 1–261

40S ribosomal protein S6-A

OrganismNot specified

UniProt P0CX37

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS6A_YEAST
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain SG； PDBConstruct 1–236； UniProt 1–236

40S ribosomal protein S7-A

OrganismNot specified

UniProt P26786

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS7A_YEAST
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain SH； PDBConstruct 1–190； UniProt 1–190

40S ribosomal protein S8-A

OrganismNot specified

UniProt P0CX39

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS8A_YEAST
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain SI； PDBConstruct 1–200； UniProt 1–200

40S ribosomal protein S9-A

OrganismNot specified

UniProt O13516

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS9A_YEAST
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain SJ； PDBConstruct 1–197； UniProt 1–197

40S ribosomal protein S11-A

OrganismNot specified

UniProt P0CX47

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS11A_YEAST
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain SL； PDBConstruct 1–156； UniProt 1–156

40S ribosomal protein S13

OrganismNot specified

UniProt P05756

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS13_YEAST
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain SN； PDBConstruct 1–151； UniProt 1–151

40S ribosomal protein S14-A

OrganismNot specified

UniProt P06367

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS14A_YEAST
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain SO； PDBConstruct 1–137； UniProt 1–137

40S ribosomal protein S22-A

OrganismNot specified

UniProt P0C0W1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS22A_YEAST
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain SW； PDBConstruct 1–130； UniProt 1–130

40S ribosomal protein S23-A

OrganismNot specified

UniProt P0CX29

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS23A_YEAST
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain SX； PDBConstruct 1–145； UniProt 1–145

40S ribosomal protein S24-A

OrganismNot specified

UniProt P0CX31

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS24A_YEAST
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain SY； PDBConstruct 1–135； UniProt 1–135

40S ribosomal protein S27-A

OrganismNot specified

UniProt P35997

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS27A_YEAST
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain Sb； PDBConstruct 1–82； UniProt 1–82

40S ribosomal protein S30-A

OrganismNot specified

UniProt P0CX33

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RS30A_YEAST
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain Se； PDBConstruct 1–63； UniProt 1–63

Pre-rRNA-processing protein PNO1

OrganismNot specified

UniProt Q99216

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PNO1_YEAST
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain CA； PDBConstruct 1–274； UniProt 1–274

18S rRNA (guanine(1575)-N(7))-methyltransferase

OrganismNot specified

UniProt P25627

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） Ribosome biogenesis protein TSR1 × 1 （Q07381） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	BUD23_YEAST
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain CB； PDBConstruct 1–275； UniProt 1–275

Ribosome biogenesis protein TSR1

OrganismNot specified

UniProt Q07381

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 17 RNA 1	18S rRNA × 1 40S ribosomal protein S1-A × 1 （P33442） 40S ribosomal protein S4-A × 1 （P0CX35） 40S ribosomal protein S6-A × 1 （P0CX37） 40S ribosomal protein S7-A × 1 （P26786） 40S ribosomal protein S8-A × 1 （P0CX39） 40S ribosomal protein S9-A × 1 （O13516） 40S ribosomal protein S11-A × 1 （P0CX47） 40S ribosomal protein S13 × 1 （P05756） 40S ribosomal protein S14-A × 1 （P06367） 40S ribosomal protein S22-A × 1 （P0C0W1） 40S ribosomal protein S23-A × 1 （P0CX29） 40S ribosomal protein S24-A × 1 （P0CX31） 40S ribosomal protein S27-A × 1 （P35997） 40S ribosomal protein S30-A × 1 （P0CX33） Pre-rRNA-processing protein PNO1 × 1 （Q99216） 18S rRNA (guanine(1575)-N(7))-methyltransferase × 1 （P25627） ZINC ION × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	TSR1_YEAST
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain CC； PDBConstruct 1–788； UniProt 1–788

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7wtq
Deposition date deposition_date	2022-02-05
Structure title title	Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-2 (without Rps2)
Keywords keywords	ribosome biogenesis, 40S ribosome, RIBOSOME; RIBOSOME
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7wtq__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7wtq__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

7wtq__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	63.16 Å
Rg (electron density)	64.73 Å
Total Rg	64.58 Å
Atom count	51309
Residues	4334
Excluded volume	817930 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`7wtq__assembly_1__model_1`	octadecameric （18）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (19)

Entity 1 Entity 1 polymer

Description description	18S rRNA
Molecular weight formula_weight	579761.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	C2
Atom count atom_count	51309

UAUCUGGUUGAUCCUGCCAGUAGUCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUAUAAGCAAUUUAUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUCGUUUAUUUGAUAGUUCCUUUACUACAUGGUAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCUUAAAAUCUCGACCCUUUGGAAGAGAUGUAUUUAUUAGAUAAAAAAUCAAUGUCUUCGGACUCUUUGAUGAUUCAUAAUAACUUUUCGAAUCGCAUGGCCUUGUGCUGGCGAUGGUUCAUUCAAAUUUCUGCCCUAUCAACUUUCGAUGGUAGGAUAGUGGCCUACCAUGGUUUCAACGGGUAACGGGGAAUAAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCGCGCAAAUUACCCAAUCCUAAUUCAGGGAGGUAGUGACAAUAAAUAACGAUACAGGGCCCAUUCGGGUCUUGUAAUUGGAAUGAGUACAAUGUAAAUACCUUAACGAGGAACAAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUGUUGCAGUUAAAAAGCUCGUAGUUGAACUUUGGGCCCGGUUGGCCGGUCCGAUUUUUUCGUGUACUGGAUUUCCAACGGGGCCUUUCCUUCUGGCUAACCUUGAGUCCUUGUGGCUCUUGGCGAACCAGGACUUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCGUAUUGCUCGAAUAUAUUAGCAUGGAAUAAUAGAAUAGGACGUUUGGUUCUAUUUUGUUGGUUUCUAGGACCAUCGUAAUGAUUAAUAGGGACGGUCGGGGGCAUCAGUAUUCAAUUGUCAGAGGUGAAAUUCUUGGAUUUAUUGAAGACUAACUACUGCGAAAGCAUUUGCCAAGGACGUUUUCAUUAAUCAAGAACGAAAGUUAGGGGAUCGAAGAUGAUCAGAUACCGUCGUAGUCUUAACCAUAAACUAUGCCGACUAGGGAUCGGGUGGUGUUUUUUUAAUGACCCACUCGGCACCUUACGAGAAAUCAAAGUCUUUGGGUUCUGGGGGGAGUAUGGUCGCAAGGCUGAAACUUAAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGGAAACUCACCAGGUCCAGACACAAUAAGGAUUGACAGAUUGAGAGCUCUUUCUUGAUUUUGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGUGAUUUGUCUGCUUAAUUGCGAUAACGAACGAGACCUUAACCUACUAAAUAGUGGUGCUAGCAUUUGCUGGUUAUCCACUUCUUAGAGGGACUAUCGGUUUCAAGCCGAUGGAAGUUUGAGGCAAUAACAGGUCUGUGAUGCCCUUAGACGUUCUGGGCCGCACGCGCGCUACACUGACGGAGCCAGCGAGUCUAACCUUGGCCGAGAGGUCUUGGUAAUCUUGUGAAACUCCGUCGUGCUGGGGAUAGAGCAUUGUAAUUAUUGCUCUUCAACGAGGAAUUCCUAGUAAGCGCAAGUCAUCAGCUUGCGUUGAUUACGUCCCUGCCCUUUGUACACACCGCCCGUCGCUAGUACCGAUUGAAUGGCUUAGUGAGGCCUCAGGAUCUGCUUAGAGAAGGGGGCAACUCCAUCUCAGAGCGGAGAAUUUGGACAAACUUGGUCAUUUAGAGGAACUAAAAGUCGUAACAAGGUUUCCGUAGGUGAACCUGCGGAAGGAUCAUUA

Entity 2 Entity 2 polymer

Description description	40S ribosomal protein S1-A
Molecular weight formula_weight	28798.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SB

MAVGKNKRLSKGKKGQKKRVVDPFTRKEWFDIKAPSTFENRNVGKTLVNKSTGLKSASDALKGRVVEVCLADLQGSEDHSFRKIKLRVDEVQGKNLLTNFHGMDFTTDKLRSMVRKWQTLIEANVTVKTSDDYVLRIFAIAFTRKQANQVKRHSYAQSSHIRAIRKVISEILTKEVQGSTLAQLTSKLIPEVINKEIENATKDIFPLQNIHVRKVKLLKQPKFDVGALMALHGEGSGEEKGKKVTGFKDEVLETV

Entity 3 Entity 3 polymer

Description description	40S ribosomal protein S4-A
Molecular weight formula_weight	29469.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SE

MARGPKKHLKRLAAPHHWLLDKLSGCYAPRPSAGPHKLRESLPLIVFLRNRLKYALNGREVKAILMQRHVKVDGKVRTDTTYPAGFMDVITLDATNENFRLVYDVKGRFAVHRITDEEASYKLGKVKKVQLGKKGVPYVVTHDGRTIRYPDPNIKVNDTVKIDLASGKITDFIKFDAGKLVYVTGGRNLGRIGTIVHKERHDGGFDLVHIKDSLDNTFVTRLNNVFVIGEQGKPYISLPKGKGIKLSIAEERDRRRAQQGL

Entity 4 Entity 4 polymer

Description description	40S ribosomal protein S6-A
Molecular weight formula_weight	27054.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SG

MKLNISYPVNGSQKTFEIDDEHRIRVFFDKRIGQEVDGEAVGDEFKGYVFKISGGNDKQGFPMKQGVLLPTRIKLLLTKNVSCYRPRRDGERKRKSVRGAIVGPDLAVLALVIVKKGEQELEGLTDTTVPKRLGPKRANNIRKFFGLSKEDDVRDFVIRREVTKGEKTYTKAPKIQRLVTPQRLQRKRHQRALKVRNAQAQREAAAEYAQLLAKRLSERKAEKAEIRKRRASSLKA

Entity 5 Entity 5 polymer

Description description	40S ribosomal protein S7-A
Molecular weight formula_weight	21658.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SH

MSAPQAKILSQAPTELELQVAQAFVELENSSPELKAELRPLQFKSIREIDVAGGKKALAIFVPVPSLAGFHKVQTKLTRELEKKFQDRHVIFLAERRILPKPSRTSRQVQKRPRSRTLTAVHDKILEDLVFPTEIVGKRVRYLVGGNKIQKVLLDSKDVQQIDYKLESFQAVYNKLTGKQIVFEIPSETH

Entity 6 Entity 6 polymer

Description description	40S ribosomal protein S8-A
Molecular weight formula_weight	22537.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SI

MGISRDSRHKRSATGAKRAQFRKKRKFELGRQPANTKIGAKRIHSVRTRGGNKKYRALRIETGNFSWASEGISKKTRIAGVVYHPSNNELVRTNTLTKAAIVQIDATPFRQWFEAHYGQTLGKKKNVKEEETVAKSKNAERKWAARAASAKIESSVESQFSAGRLYACISSRPGQSGRCDGYILEGEELAFYLRRLTAKK

Entity 7 Entity 7 polymer

Description description	40S ribosomal protein S9-A
Molecular weight formula_weight	22487.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SJ

MPRAPRTYSKTYSTPKRPYESSRLDAELKLAGEFGLKNKKEIYRISFQLSKIRRAARDLLTRDEKDPKRLFEGNALIRRLVRVGVLSEDKKKLDYVLALKVEDFLERRLQTQVYKLGLAKSVHHARVLITQRHIAVGKQIVNIPSFMVRLDSEKHIDFAPTSPFGGARPGRVARRNAARKAEASGEAADEADEADEE

Entity 8 Entity 8 polymer

Description description	40S ribosomal protein S11-A
Molecular weight formula_weight	17785.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SL

MSTELTVQSERAFQKQPHIFNNPKVKTSKRTKRWYKNAGLGFKTPKTAIEGSYIDKKCPFTGLVSIRGKILTGTVVSTKMHRTIVIRRAYLHYIPKYNRYEKRHKNVPVHVSPAFRVQVGDIVTVGQCRPISKTVRFNVVKVSAAAGKANKQFAKF

Entity 9 Entity 9 polymer

Description description	40S ribosomal protein S13
Molecular weight formula_weight	17059.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SN

MGRMHSAGKGISSSAIPYSRNAPAWFKLSSESVIEQIVKYARKGLTPSQIGVLLRDAHGVTQARVITGNKIMRILKSNGLAPEIPEDLYYLIKKAVSVRKHLERNRKDKDAKFRLILIESRIHRLARYYRTVAVLPPNWKYESATASALVN

Entity 10 Entity 10 polymer

Description description	40S ribosomal protein S14-A
Molecular weight formula_weight	14562.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SO

MSNVVQARDNSQVFGVARIYASFNDTFVHVTDLSGKETIARVTGGMKVKADRDESSPYAAMLAAQDVAAKCKEVGITAVHVKIRATGGTRTKTPGPGGQAALRALARSGLRIGRIEDVTPVPSDSTRKKGGRRGRRL

Entity 11 Entity 11 polymer

Description description	40S ribosomal protein S22-A
Molecular weight formula_weight	14650.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SW

MTRSSVLADALNAINNAEKTGKRQVLIRPSSKVIIKFLQVMQKHGYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNVKIGDIEKWTANLLPARQFGYVILTTSAGIMDHEEARRKHVSGKILGFVY

Entity 12 Entity 12 polymer

Description description	40S ribosomal protein S23-A
Molecular weight formula_weight	16073.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SX

MGKGKPRGLNSARKLRVHRRNNRWAENNYKKRLLGTAFKSSPFGGSSHAKGIVLEKLGIESKQPNSAIRKCVRVQLIKNGKKVTAFVPNDGCLNFVDENDEVLLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS

Entity 13 Entity 13 polymer

Description description	40S ribosomal protein S24-A
Molecular weight formula_weight	15362.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	SY

MSDAVTIRTRKVISNPLLARKQFVVDVLHPNRANVSKDELREKLAEVYKAEKDAVSVFGFRTQFGGGKSVGFGLVYNSVAEAKKFEPTYRLVRYGLAEKVEKASRQQRKQKKNRDKKIFGTGKRLAKKVARRNAD

Entity 14 Entity 14 polymer

Description description	40S ribosomal protein S27-A
Molecular weight formula_weight	8893.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Sb

MVLVQDLLHPTAASEARKHKLKTLVQGPRSYFLDVKCPGCLNITTVFSHAQTAVTCESCSTILCTPTGGKAKLSEGTSFRRK

Entity 15 Entity 15 polymer

Description description	40S ribosomal protein S30-A
Molecular weight formula_weight	7137.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Se

MAKVHGSLARAGKVKSQTPKVEKTEKPKKPKGRAYKRLLYTRRFVNVTLVNGKRRMNPGPSVQ

Entity 16 Entity 16 polymer

Description description	Pre-rRNA-processing protein PNO1
Molecular weight formula_weight	30380.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	CA

MVAPTALKKATVTPVSGQDGGSSRIIGINNTESIDEDDDDDVLLDDSDNNTAKEEVEGEEGSRKTHESKTVVVDDQGKPRFTSASKTQGNKIKFESRKIMVPPHRMTPLRNSWTKIYPPLVEHLKLQVRMNLKTKSVELRTNPKFTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDLYIETFEVKDVKTLTGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPGKVYGNLRTVASRLKERY

Entity 17 Entity 17 polymer

Description description	18S rRNA (guanine(1575)-N(7))-methyltransferase
Molecular weight formula_weight	30788.2 kDa
EC 编号 ec_number	2.1.1.309
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	CB

MSRPEELAPPEIFYNDSEAHKYTGSTRVQHIQAKMTLRALELLNLQPCSFILDIGCGSGLSGEILTQEGDHVWCGLDISPSMLATGLSRELEGDLMLQDMGTGIPFRAGSFDAAISISAIQWLCNADTSYNDPKQRLMRFFNTLYAALKKGGKFVAQFYPKNDDQVDDILQSAKVAGFSGGLVVDDPESKKNKKYYLVLSSGAPPQGEEQVNLDGVTMDEENVNLKKQLRQRLKGGKDKESAKSFILRKKELMKRRGRKVAKDSKFTGRKRRHRF

Entity 18 Entity 18 polymer

Description description	Ribosome biogenesis protein TSR1
Molecular weight formula_weight	90876.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	CC

MAGHSHRSSLKNGHKSYKSKHASKGALKRLYKGKVEKEPVGTGKPDKQVSKLQRKNKAKQLRAQRILDSIENRKLFEGKNGAAKIITIVPLVNDLDPLDILYKLLKCADDEGIMVQEVDSKRIFNVHIKKFKSNLKIIIPDMTNFLNILDCAKVADFVVFGLSGVQEVDEEFGEQIIRALELQGIASYIGVISNLSAVHEKEKFQLDVKQSLESYFKHFFPSEERVYNLEKNSDALNVLRTLCQRLPRSINWRDNRGYVVADFVDFVETSPDSGDLVIEGTVRGIGFNANRLVHIPDFGDFQLNKIEKISESSQKRKIIKEKATDSLSLELDLQTVFESNMNRDTLDEYAPEGTEDWSDYDEDFEYDGLTTARYDDHGFLPGREQTSKKAAVPKGTSDYQAKWYLDDVIDANEEEEAEQTNGKDETMMEIDDEMMVEQDNEEVAGDEEYDIEDNEGFEELSPEEEERQLREFRDMEKEDREFPDEIELEPSESAIERLKRYRGLKNLYNCDWQVDEKDPSSPAEWKRLLRIGNYKNTKNRIIKETKNEAQAIAGDRIRMFIRFPKFLLEKIQDPKQLLFAVYGLLLHEHKNAVVNFSLQRWEQYDKPVPSQEPIVVQYGVRRYTIQPLFSQGSNSPNNVHKYERFLHPDTVSVATCIAPVDFTQSPAIFFKPSPTDAKNIELIGHGTFLNADHSRILAKRAILTGHPFRFHKTVVTVRYMFFRPEDVEWFKSIPLFTKSGRSGFIKESLGTHGYFKATFDGKLSAQDVVAMSLYKRMWPMPSLPWNGM

Entity 19 Entity 19 non-polymer

Description description	ZINC ION
Molecular weight formula_weight	65.4 kDa
Source method src_method	syn
Copy count entity_count	1

▶

Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	—

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	triclinic

Resolution resolution	3.70 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	39865

Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Tsr1-2 (without Rps2)

1. Protein Identity and Related Structures Protein Identity & Related Structures

40S ribosomal protein S1-A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

40S ribosomal protein S4-A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

40S ribosomal protein S6-A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

40S ribosomal protein S7-A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

40S ribosomal protein S8-A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

40S ribosomal protein S9-A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

40S ribosomal protein S11-A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

40S ribosomal protein S13

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

40S ribosomal protein S14-A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

40S ribosomal protein S22-A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer