Current Protein Identity:Q0JRZ9
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 7OG1 AP2 clathrin adaptor core in complex with cargo peptide and FCHO2 Deposited 2021-05-05 | Assembly 1 Protein heterocomplex Heteromer;Protein × 8 PDB declaration: octameric(8) Consistent with protein count |
Chain DDD
314–444(131 aa)
Chain GGG
314–444(131 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;289 K;Crystals of AP-2 in complex with FCHO2 linker and TGN38 peptide, supplemented with 10mM K Na Tartrate, grew in sitting drops with reservoir 0.1M Mg formate dehydrate, 10% to 15% PEG 3350.
The crystals were cryo-protected with 0.1M Mg formate dehydrate, 13% PEG 3350, 18-24% Glycerol and 1mg/ml of peptide.
|
Resolution 3.25 Å R-free 0.306 |
| 7OHO Crystal structure of AP2 FCHO2 chimera Deposited 2021-05-11 | Assembly 1 Insufficient information Heteromer;Protein × 4 PDB declaration: tetrameric(4) Consistent with protein count |
Chain BBB
358–444(87 aa)
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6.2;289 K;18% PEG 12000 0.1M Na/K phosphate pH 6.2 0.2M NaCl 4mM DTT in the presence of 3-fold molar excess of IP6.
|
Resolution 2.88 Å R-free 0.273 |
| 7OHZ Crystal structure of AP2 Mu2 - FCHO2 chimera (His6-tagged) Deposited 2021-05-11 | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: trimeric(3) Review required |
Chain B
316–351(36 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7;289 K;20% w/v PEG 3,350 0.2 M DL-Malic acid pH 7.0. The crystals were cryo-protected by soaking in mother liquor supplemented with 30-32% glycerol.
|
Resolution 2.27 Å R-free 0.314 |
| 7OHZ Crystal structure of AP2 Mu2 - FCHO2 chimera (His6-tagged) Deposited 2021-05-11 | Assembly 2 Insufficient information Monomer;Protein × 1 PDB declaration: trimeric(3) Review required |
Chain A
316–351(36 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7;289 K;20% w/v PEG 3,350 0.2 M DL-Malic acid pH 7.0. The crystals were cryo-protected by soaking in mother liquor supplemented with 30-32% glycerol.
|
Resolution 2.27 Å R-free 0.314 |
| 7OI5 Crystal structure of AP2 Mu2 - FCHO2 chimera (GST cleaved) Deposited 2021-05-11 | Assembly 1 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain B
316–351(36 aa)
|
Not recorded | GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 9.1;289 K;20% w/v PEG 3350, 0.2 M Sodium phosphate dibasic dehydrate pH 9.1. The crystal was cryo-protected by soaking in mother liquor supplemented with 25% glycerol.
|
Resolution 2.61 Å R-free 0.297 |
| 7OI5 Crystal structure of AP2 Mu2 - FCHO2 chimera (GST cleaved) Deposited 2021-05-11 | Assembly 2 Insufficient information Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain D
316–351(36 aa)
|
Not recorded | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 9.1;289 K;20% w/v PEG 3350, 0.2 M Sodium phosphate dibasic dehydrate pH 9.1. The crystal was cryo-protected by soaking in mother liquor supplemented with 25% glycerol.
|
Resolution 2.61 Å R-free 0.297 |
| 7OIQ Crystal structure of AP2 Mu2 in complex with FCHO2 WxxPhi motif (C2 crystal form) Deposited 2021-05-12 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain DDD
422–432(11 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;289 K;30mM Magnesium chloride hexahydrate, 30mM Calcium chloride dihydrate, 100mM Sodium HEPES MOPS (acid) pH 7.5, 20% v/v Ethylene glycol; 10 % w/v PEG 8000
|
Resolution 1.85 Å R-free 0.209 |
| 7OIQ Crystal structure of AP2 Mu2 in complex with FCHO2 WxxPhi motif (C2 crystal form) Deposited 2021-05-12 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain CCC
422–432(11 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7.5;289 K;30mM Magnesium chloride hexahydrate, 30mM Calcium chloride dihydrate, 100mM Sodium HEPES MOPS (acid) pH 7.5, 20% v/v Ethylene glycol; 10 % w/v PEG 8000
|
Resolution 1.85 Å R-free 0.209 |
| 7OIT Crystal structure of AP2 Mu2 in complex with FCHO2 WxxPhi motif (P3221 crystal form) Deposited 2021-05-12 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain BBB
422–432(11 aa)
|
Not recorded | GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 6.5;289 K;20mM Sodium formate; 20mM Ammonium acetate; 20mM Sodium citrate tribasic dihydrate; 20mM Sodium potassium tartrate tetrahydrate; 20mM Sodium oxamate, 100mM Imidazole MES monohydrate pH6.5 , 20% v/v Glycerol; 10% w/v PEG 4000
|
Resolution 1.65 Å R-free 0.202 |