当前蛋白身份:Q15149
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差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 1MB8 Crystal Structure of the actin binding domain of plectin 提交 2002-08-02 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
59–293(235 aa)
片段:Residues 59-293
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION | mmCIF 未提供已读取条件 | 分辨率 2.15 Å R-free 0.261 |
| 2N03 Solution NMR Structure plectin repeat domain 6 (4403-4606) of Plectin from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR6354E 提交 2015-03-04 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
4403–4606(204 aa)
片段:residues 4403-4606
|
未记录 | 未记录非水小分子 | SOLUTION NMR |
NMR测量条件
pH 6.5;298 K;离子强度(mmCIF原始值)100;压力 ambient
NMR样品组成
1 mM [U-100% 13C; U-100% 15N; U-2H; ILV-1H] protein, 20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02 mM NaN3, 50 uM DSS, 1 ratio protease inhibitor, 90/10 % H2O/D2O, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR样品组成
1 mM [U-100% 13C; U-100% 15N] protein, 20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02 mM NaN3, 50 uM DSS, 1 ratio protease inhibitor, 90/10 % H2O/D2O, 90% H2O/10% D2O | 90% H2O/10% D2O
NMR样品组成
1 mM [U-5% 13C; U-100% 15N] protein, 20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02 mM NaN3, 50 uM DSS, 1 ratio protease inhibitor, 90/10 % H2O/D2O, 90% H2O/10% D2O | 90% H2O/10% D2O
|
分辨率未提供 |
| 3F7P Crystal structure of a complex between integrin beta4 and plectin 提交 2008-11-10 | Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 A
1–293(293 aa)
片段:Actin-binding domain, residues 1-293
|
未记录 | EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION;pH 5.8;298 K;0.1M Sodium Acetate, 0.2M CaCl2, 10% PEG 6000, pH 5.8, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.75 Å R-free 0.257 |
| 3F7P Crystal structure of a complex between integrin beta4 and plectin 提交 2008-11-10 | Assembly 2 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 B
1–293(293 aa)
片段:Actin-binding domain, residues 1-293
|
未记录 | EDO 1,2-ETHANEDIOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION;pH 5.8;298 K;0.1M Sodium Acetate, 0.2M CaCl2, 10% PEG 6000, pH 5.8, VAPOR DIFFUSION, temperature 298K
|
分辨率 2.75 Å R-free 0.257 |
| 3PDY Structure of the third and fourth spectrin repeats of the plakin domain of plectin 提交 2010-10-25 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
653–858(206 aa)
片段:SPECTRIN REPEATS 3 AND 4 (UNP Residues 653-848)
|
未记录 | EDO 1,2-ETHANEDIOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.5;297 K;0.1M HEPES, 17% PEG 4000, 8% ISOPROPANOL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
分辨率 2.22 Å R-free 0.248 |
| 3PDY Structure of the third and fourth spectrin repeats of the plakin domain of plectin 提交 2010-10-25 | Assembly 2 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 B
653–858(206 aa)
片段:SPECTRIN REPEATS 3 AND 4 (UNP Residues 653-848)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.5;297 K;0.1M HEPES, 17% PEG 4000, 8% ISOPROPANOL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
分辨率 2.22 Å R-free 0.248 |
| 3PDY Structure of the third and fourth spectrin repeats of the plakin domain of plectin 提交 2010-10-25 | Assembly 3 蛋白同源多聚体 同源多聚体;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 A
653–858(206 aa)
片段:SPECTRIN REPEATS 3 AND 4 (UNP Residues 653-848)
链 B
653–858(206 aa)
片段:SPECTRIN REPEATS 3 AND 4 (UNP Residues 653-848)
|
未记录 | EDO 1,2-ETHANEDIOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.5;297 K;0.1M HEPES, 17% PEG 4000, 8% ISOPROPANOL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K
|
分辨率 2.22 Å R-free 0.248 |
| 3PE0 Structure of the central region of the plakin domain of plectin 提交 2010-10-25 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
750–1028(279 aa)
片段:SPECTRIN REPEATS 4 AND 5, SH3 (UNP Residues 747-918)
|
未记录 | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION;pH 7.2;277 K;0.1 M imidazole, 0.2 M calcium acetate, 9% PEG 8000, 2 mM DTT, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
分辨率 2.95 Å R-free 0.265 |
| 3PE0 Structure of the central region of the plakin domain of plectin 提交 2010-10-25 | Assembly 2 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 B
750–1028(279 aa)
片段:SPECTRIN REPEATS 4 AND 5, SH3 (UNP Residues 747-918)
|
未记录 | CA CALCIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION;pH 7.2;277 K;0.1 M imidazole, 0.2 M calcium acetate, 9% PEG 8000, 2 mM DTT, pH 7.2, VAPOR DIFFUSION, temperature 277K
|
分辨率 2.95 Å R-free 0.265 |
| 4GDO Structure of a fragment of the rod domain of plectin 提交 2012-08-01 | Assembly 1 蛋白同源多聚体 同源多聚体;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 A
1492–1530(39 aa)
片段:FRAGMENT OF THE ROD DOMAIN, UNP residues 1492-1530
链 B
1492–1530(39 aa)
片段:FRAGMENT OF THE ROD DOMAIN, UNP residues 1492-1530
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 4.5;298 K;0.1M sodium acetate, 2.2M sodium chloride, 0.2M lithium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.70 Å R-free 0.248 |
| 4GDO Structure of a fragment of the rod domain of plectin 提交 2012-08-01 | Assembly 2 蛋白同源多聚体 同源多聚体;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 C
1492–1530(39 aa)
片段:FRAGMENT OF THE ROD DOMAIN, UNP residues 1492-1530
链 D
1492–1530(39 aa)
片段:FRAGMENT OF THE ROD DOMAIN, UNP residues 1492-1530
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 4.5;298 K;0.1M sodium acetate, 2.2M sodium chloride, 0.2M lithium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.70 Å R-free 0.248 |
| 4GDO Structure of a fragment of the rod domain of plectin 提交 2012-08-01 | Assembly 3 蛋白同源多聚体 同源多聚体;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 E
1492–1530(39 aa)
片段:FRAGMENT OF THE ROD DOMAIN, UNP residues 1492-1530
链 F
1492–1530(39 aa)
片段:FRAGMENT OF THE ROD DOMAIN, UNP residues 1492-1530
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 4.5;298 K;0.1M sodium acetate, 2.2M sodium chloride, 0.2M lithium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
分辨率 1.70 Å R-free 0.248 |
| 4Q58 Crystal structure of the plectin 1a actin-binding domain/integrin beta 4 fragment complex 提交 2014-04-16 | Assembly 1 蛋白异源复合物 异源复合物;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 A
175–400(226 aa)
片段:actin-binding domain (UNP residues 175-400)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP;pH 6.5;295 K;20 mM HEPES, pH 6.5, 150 mM sodium formate, 7.5% PEG550 MME, 3% sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 4.00 Å R-free 0.287 |
| 4Q58 Crystal structure of the plectin 1a actin-binding domain/integrin beta 4 fragment complex 提交 2014-04-16 | Assembly 2 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 B
175–400(226 aa)
片段:actin-binding domain (UNP residues 175-400)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP;pH 6.5;295 K;20 mM HEPES, pH 6.5, 150 mM sodium formate, 7.5% PEG550 MME, 3% sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
分辨率 4.00 Å R-free 0.287 |
| 4Q59 Crystal structure of plectin 1a actin-binding domain 提交 2014-04-16 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
175–400(226 aa)
片段:actin-binding domain (UNP residues 175-400)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.5;295 K;0.05 M potassium phosphate monobasic, 20% PEG8000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 2.30 Å R-free 0.245 |
| 4Q59 Crystal structure of plectin 1a actin-binding domain 提交 2014-04-16 | Assembly 2 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 B
175–400(226 aa)
片段:actin-binding domain (UNP residues 175-400)
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.5;295 K;0.05 M potassium phosphate monobasic, 20% PEG8000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 2.30 Å R-free 0.245 |
| 5J1F Structure of the spectrin repeats 5 and 6 of the plakin domain of plectin 提交 2016-03-29 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
860–928(69 aa)
链 A
999–1116(118 aa)
|
突变:F752A,F752A 突变:F752A,F752A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP;pH 6;277 K;A protein solution at 30 mg/ml in 10 mM Tris-Hcl (pH 7.5), 50 mM NaCl, 1 mM DTT was mixed with an equal volume of crystallization solution 0.1 M Bis-Tris-Propane (pH 6.0), 18% PEG 3350, 0.2 Na malonate
|
分辨率 3.00 Å R-free 0.277 |
| 5J1F Structure of the spectrin repeats 5 and 6 of the plakin domain of plectin 提交 2016-03-29 | Assembly 2 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 B
860–928(69 aa)
链 B
999–1116(118 aa)
|
突变:F752A,F752A 突变:F752A,F752A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP;pH 6;277 K;A protein solution at 30 mg/ml in 10 mM Tris-Hcl (pH 7.5), 50 mM NaCl, 1 mM DTT was mixed with an equal volume of crystallization solution 0.1 M Bis-Tris-Propane (pH 6.0), 18% PEG 3350, 0.2 Na malonate
|
分辨率 3.00 Å R-free 0.277 |
| 5J1G Structure of the spectrin repeats 7 and 8 of the plakin domain of plectin 提交 2016-03-29 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1004–1233(230 aa)
片段:UNP residues 1004-1233
|
未记录 | 1PE PENTAETHYLENE GLYCOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP;pH 7.7;298 K;A protein solution at 27 mg/ml in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl was mixed with an equal volume of crystallization solution 50 mM Tris-HCl (pH 7.7), 0.2 M NaF, 18% PEG 3350
|
分辨率 1.80 Å R-free 0.218 |
| 5J1G Structure of the spectrin repeats 7 and 8 of the plakin domain of plectin 提交 2016-03-29 | Assembly 2 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 B
1004–1233(230 aa)
片段:UNP residues 1004-1233
|
未记录 | 1PE PENTAETHYLENE GLYCOL × 1 EDO 1,2-ETHANEDIOL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP;pH 7.7;298 K;A protein solution at 27 mg/ml in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl was mixed with an equal volume of crystallization solution 50 mM Tris-HCl (pH 7.7), 0.2 M NaF, 18% PEG 3350
|
分辨率 1.80 Å R-free 0.218 |
| 5J1H Structure of the spectrin repeats 5 and 6 of the plakin domain of plectin 提交 2016-03-29 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
860–928(69 aa)
链 A
999–1116(118 aa)
|
突变:F752A,F752A 突变:F752A,F752A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.5;277 K;Protein at 30 mg/ml in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT was mixed with an equal volume of the crystallization solution 0.2 M Na/K tartrate, 20% PEG3350
|
分辨率 2.80 Å R-free 0.269 |
| 5J1H Structure of the spectrin repeats 5 and 6 of the plakin domain of plectin 提交 2016-03-29 | Assembly 2 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 B
860–928(69 aa)
链 B
999–1116(118 aa)
|
突变:F752A,F752A 突变:F752A,F752A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.5;277 K;Protein at 30 mg/ml in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT was mixed with an equal volume of the crystallization solution 0.2 M Na/K tartrate, 20% PEG3350
|
分辨率 2.80 Å R-free 0.269 |
| 5J1I Structure of the spectrin repeats 7, 8, and 9 of the plakin domain of plectin 提交 2016-03-29 | Assembly 1 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 A
1004–1372(369 aa)
片段:UNP residues 1104-1372
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP;pH 8;298 K;Protein solution at 12 mg/ml in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT was mixed with crystallization solution 0.1 M Bis-Tris-propane (pH 8.0), 16% PEG 3350, 0.3 M Na/K tartrate
|
分辨率 2.80 Å R-free 0.322 |
| 5J1I Structure of the spectrin repeats 7, 8, and 9 of the plakin domain of plectin 提交 2016-03-29 | Assembly 2 蛋白单体 单体;蛋白 × 1 PDB 声明:monomeric(1) 与蛋白数一致 |
链 B
1004–1372(369 aa)
片段:UNP residues 1104-1372
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP;pH 8;298 K;Protein solution at 12 mg/ml in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT was mixed with crystallization solution 0.1 M Bis-Tris-propane (pH 8.0), 16% PEG 3350, 0.3 M Na/K tartrate
|
分辨率 2.80 Å R-free 0.322 |