Current Protein Identity：Q16658
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1DFC CRYSTAL STRUCTURE OF HUMAN FASCIN, AN ACTIN-CROSSLINKING PROTEIN Deposited 1999-11-18 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
Fragment：FULL-LENGTH PROTEIN
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 4000, hepes, dtt, sodium azide , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.90 Å R-free 0.268 |
| 1DFC CRYSTAL STRUCTURE OF HUMAN FASCIN, AN ACTIN-CROSSLINKING PROTEIN Deposited 1999-11-18 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–493（493 aa）
Fragment：FULL-LENGTH PROTEIN
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；PEG 4000, hepes, dtt, sodium azide , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 2.90 Å R-free 0.268 |
| 3LLP 1.8 Angstrom human fascin 1 crystal structure Deposited 2010-01-29 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | GOL GLYCEROL × 3 K POTASSIUM ION × 1 BR BROMIDE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100mM Hepes, pH 7.5, 20% PEG 4000, 2% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.220 |
| 3LLP 1.8 Angstrom human fascin 1 crystal structure Deposited 2010-01-29 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–493（493 aa）
|
Not recorded | GOL GLYCEROL × 2 K POTASSIUM ION × 1 BR BROMIDE ION × 4 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；100mM Hepes, pH 7.5, 20% PEG 4000, 2% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.220 |
| 3P53 Structure of fascin Deposited 2010-10-07 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | GOL GLYCEROL × 4 12P DODECAETHYLENE GLYCOL × 1 1PE PENTAETHYLENE GLYCOL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；24% PEG 3350, 0.2 M Lithium Acetate, 1mM DTT, 4% glycerol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.220 |
| 3P53 Structure of fascin Deposited 2010-10-07 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–493（493 aa）
|
Not recorded | GOL GLYCEROL × 4 1PE PENTAETHYLENE GLYCOL × 2 PG0 2-(2-METHOXYETHOXY)ETHANOL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；24% PEG 3350, 0.2 M Lithium Acetate, 1mM DTT, 4% glycerol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.220 |
| 4GOV The crystal structure of human fascin 1 S39D mutant Deposited 2012-08-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Mutation：S39D | BR BROMIDE ION × 2 CL CHLORIDE ION × 5 GOL GLYCEROL × 3 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100 mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.234 |
| 4GOV The crystal structure of human fascin 1 S39D mutant Deposited 2012-08-20 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–493（493 aa）
|
Mutation：S39D | BR BROMIDE ION × 4 CL CHLORIDE ION × 8 GOL GLYCEROL × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100 mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.234 |
| 4GOV The crystal structure of human fascin 1 S39D mutant Deposited 2012-08-20 | Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–493（493 aa）
Chain B
1–493（493 aa）
|
Mutation：S39D Mutation：S39D | BR BROMIDE ION × 12 CL CHLORIDE ION × 26 GOL GLYCEROL × 20 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100 mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.20 Å R-free 0.234 |
| 4GOY The crystal structure of human fascin 1 K41A mutant Deposited 2012-08-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Mutation：K41A | BR BROMIDE ION × 3 CL CHLORIDE ION × 4 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.245 |
| 4GOY The crystal structure of human fascin 1 K41A mutant Deposited 2012-08-20 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–493（493 aa）
|
Mutation：K41A | BR BROMIDE ION × 5 CL CHLORIDE ION × 6 GOL GLYCEROL × 3 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.245 |
| 4GOY The crystal structure of human fascin 1 K41A mutant Deposited 2012-08-20 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–493（493 aa）
Chain B
1–493（493 aa）
|
Mutation：K41A Mutation：K41A | BR BROMIDE ION × 8 CL CHLORIDE ION × 10 GOL GLYCEROL × 6 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.245 |
| 4GOY The crystal structure of human fascin 1 K41A mutant Deposited 2012-08-20 | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–493（493 aa）
|
Mutation：K41A | BR BROMIDE ION × 10 CL CHLORIDE ION × 12 GOL GLYCEROL × 6 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.245 |
| 4GP0 The crystal structure of human fascin 1 R149A K150A R151A mutant Deposited 2012-08-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Mutation：R149A K150A R151A | BR BROMIDE ION × 1 CL CHLORIDE ION × 11 GOL GLYCEROL × 4 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.249 |
| 4GP0 The crystal structure of human fascin 1 R149A K150A R151A mutant Deposited 2012-08-20 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–493（493 aa）
|
Mutation：R149A K150A R151A | BR BROMIDE ION × 6 CL CHLORIDE ION × 18 GOL GLYCEROL × 6 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.249 |
| 4GP0 The crystal structure of human fascin 1 R149A K150A R151A mutant Deposited 2012-08-20 | Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–493（493 aa）
Chain B
1–493（493 aa）
|
Mutation：R149A K150A R151A Mutation：R149A K150A R151A | BR BROMIDE ION × 14 CL CHLORIDE ION × 58 GOL GLYCEROL × 20 DTT 2,3-DIHYDROXY-1,4-DITHIOBUTANE × 6 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.249 |
| 4GP3 The crystal structure of human fascin 1 K358A mutant Deposited 2012-08-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Mutation：K358A | BR BROMIDE ION × 3 CL CHLORIDE ION × 5 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.25 Å R-free 0.252 |
| 4GP3 The crystal structure of human fascin 1 K358A mutant Deposited 2012-08-20 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–493（493 aa）
|
Mutation：K358A | BR BROMIDE ION × 4 CL CHLORIDE ION × 13 GOL GLYCEROL × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.25 Å R-free 0.252 |
| 4GP3 The crystal structure of human fascin 1 K358A mutant Deposited 2012-08-20 | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–493（493 aa）
Chain B
1–493（493 aa）
|
Mutation：K358A Mutation：K358A | BR BROMIDE ION × 7 CL CHLORIDE ION × 18 GOL GLYCEROL × 7 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.25 Å R-free 0.252 |
| 4GP3 The crystal structure of human fascin 1 K358A mutant Deposited 2012-08-20 | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–493（493 aa）
|
Mutation：K358A | BR BROMIDE ION × 8 CL CHLORIDE ION × 26 GOL GLYCEROL × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes, 16% PEG 4000, 1% isopropanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.25 Å R-free 0.252 |
| 6B0T Structural Insights into the Induced-fit Inhibition of Fascin by a Small Molecule Deposited 2017-09-15 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
7–493（487 aa）
Fragment：residues 7-493
|
Not recorded | C7V 4-methyl-N-(1-{[4-(trifluoromethyl)phenyl]methyl}-1H-indazol-3-yl)-1,2-oxazole-5-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；24% PEG 4000, 50mM Tris HCL (pH 8), 3.3% glycerol, 142mM NaCl, 5mM MgCl2, 10mM CaCl2 and 0.004% NaN3
|
Resolution 2.80 Å R-free 0.303 |
| 6B0T Structural Insights into the Induced-fit Inhibition of Fascin by a Small Molecule Deposited 2017-09-15 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
7–493（487 aa）
Fragment：residues 7-493
|
Not recorded | C7V 4-methyl-N-(1-{[4-(trifluoromethyl)phenyl]methyl}-1H-indazol-3-yl)-1,2-oxazole-5-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；24% PEG 4000, 50mM Tris HCL (pH 8), 3.3% glycerol, 142mM NaCl, 5mM MgCl2, 10mM CaCl2 and 0.004% NaN3
|
Resolution 2.80 Å R-free 0.303 |
| 6B0T Structural Insights into the Induced-fit Inhibition of Fascin by a Small Molecule Deposited 2017-09-15 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
7–493（487 aa）
Fragment：residues 7-493
|
Not recorded | C7V 4-methyl-N-(1-{[4-(trifluoromethyl)phenyl]methyl}-1H-indazol-3-yl)-1,2-oxazole-5-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；24% PEG 4000, 50mM Tris HCL (pH 8), 3.3% glycerol, 142mM NaCl, 5mM MgCl2, 10mM CaCl2 and 0.004% NaN3
|
Resolution 2.80 Å R-free 0.303 |
| 6B0T Structural Insights into the Induced-fit Inhibition of Fascin by a Small Molecule Deposited 2017-09-15 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
7–493（487 aa）
Fragment：residues 7-493
|
Not recorded | C7V 4-methyl-N-(1-{[4-(trifluoromethyl)phenyl]methyl}-1H-indazol-3-yl)-1,2-oxazole-5-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；24% PEG 4000, 50mM Tris HCL (pH 8), 3.3% glycerol, 142mM NaCl, 5mM MgCl2, 10mM CaCl2 and 0.004% NaN3
|
Resolution 2.80 Å R-free 0.303 |
| 6B0T Structural Insights into the Induced-fit Inhibition of Fascin by a Small Molecule Deposited 2017-09-15 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
7–493（487 aa）
Fragment：residues 7-493
|
Not recorded | C7V 4-methyl-N-(1-{[4-(trifluoromethyl)phenyl]methyl}-1H-indazol-3-yl)-1,2-oxazole-5-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；24% PEG 4000, 50mM Tris HCL (pH 8), 3.3% glycerol, 142mM NaCl, 5mM MgCl2, 10mM CaCl2 and 0.004% NaN3
|
Resolution 2.80 Å R-free 0.303 |
| 6B0T Structural Insights into the Induced-fit Inhibition of Fascin by a Small Molecule Deposited 2017-09-15 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain F
7–493（487 aa）
Fragment：residues 7-493
|
Not recorded | C7V 4-methyl-N-(1-{[4-(trifluoromethyl)phenyl]methyl}-1H-indazol-3-yl)-1,2-oxazole-5-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；292 K；24% PEG 4000, 50mM Tris HCL (pH 8), 3.3% glycerol, 142mM NaCl, 5mM MgCl2, 10mM CaCl2 and 0.004% NaN3
|
Resolution 2.80 Å R-free 0.303 |
| 6I0Z CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 1 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | GZQ ~{N}-(2,4-dichlorophenyl)-~{N}-methyl-ethanamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；16-20% PEG 3350, 200 mM LiAc, 4% glycerol
|
Resolution 1.77 Å R-free 0.227 |
| 6I0Z CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 1 Deposited 2018-10-27 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–493（493 aa）
|
Not recorded | GZQ ~{N}-(2,4-dichlorophenyl)-~{N}-methyl-ethanamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；292 K；16-20% PEG 3350, 200 mM LiAc, 4% glycerol
|
Resolution 1.77 Å R-free 0.227 |
| 6I10 CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 2 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | GZK 1-[(3~{R})-1,1-bis(oxidanylidene)thiolan-3-yl]-5-[(3,4-dichlorophenyl)methyl]pyrazolo[3,4-d]pyrimidin-4-one × 1 ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 2.10 Å R-free 0.287 |
| 6I11 CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 3 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ACT ACETATE ION × 1 H0H ~{N}-(1-methylpyrazol-4-yl)-1-oxidanylidene-2-(phenylmethyl)isoquinoline-4-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 1.67 Å R-free 0.247 |
| 6I12 CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 5 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ACT ACETATE ION × 1 H08 2-[(4-chlorophenyl)methyl]-~{N}-(1-methylpyrazol-4-yl)-1-oxidanylidene-isoquinoline-4-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 1.65 Å R-free 0.208 |
| 6I13 CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 7 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 3 H0Q 2-[(3-chlorophenyl)methyl]-~{N}-(1-methylpyrazol-4-yl)-1-oxidanylidene-isoquinoline-4-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 1.79 Å R-free 0.227 |
| 6I14 CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 9 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ACT ACETATE ION × 1 GZN 2-[(3,4-dichlorophenyl)methyl]-~{N}-(1-methylpyrazol-4-yl)-1-oxidanylidene-isoquinoline-4-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 1.73 Å R-free 0.216 |
| 6I15 CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 11 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 ACT ACETATE ION × 1 GZT 1-[[2,2-bis(fluoranyl)-1,3-benzodioxol-5-yl]methyl]-~{N}-methyl-2-oxidanylidene-pyridine-3-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 1.91 Å R-free 0.243 |
| 6I16 CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 15 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ACT ACETATE ION × 1 H0B 6-[(3,4-dichlorophenyl)methyl]-~{N}-(1-methylpyrazol-4-yl)-5-oxidanylidene-1,6-naphthyridine-8-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 2.00 Å R-free 0.239 |
| 6I17 CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH COMPOUND 24 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 1 GZW 2-[(3,4-dichlorophenyl)methyl]-~{N}-(1-methylpyrazol-4-yl)-1-oxidanylidene-6-piperidin-4-yl-2,7-naphthyridine-4-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 1.56 Å R-free 0.240 |
| 6I18 CRYSTAL STRUCTURE OF FASCIN IN COMPLEX WITH BDP-13176 Deposited 2018-10-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ACT ACETATE ION × 1 EDO 1,2-ETHANEDIOL × 5 H0N 5-[(3,4-dichlorophenyl)methyl]-4-oxidanylidene-1-piperidin-4-yl-~{N}-pyridin-4-yl-pyrazolo[4,3-c]pyridine-7-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 1.49 Å R-free 0.195 |
| 7ZAU Fascin-1 in complex with Nb 3E11 Deposited 2022-03-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 6 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；0.02 M D-glucose, D-mannose, D-galactose, L-fucose, D-xylose, N-acetyl-D-glucosamine; 0.1 M MES/imidazole pH 6.5, 10% PEG 8000, 20% ethylene glycol
|
Resolution 2.20 Å R-free 0.290 |
| 7ZAU Fascin-1 in complex with Nb 3E11 Deposited 2022-03-22 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–493（493 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 2 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；0.02 M D-glucose, D-mannose, D-galactose, L-fucose, D-xylose, N-acetyl-D-glucosamine; 0.1 M MES/imidazole pH 6.5, 10% PEG 8000, 20% ethylene glycol
|
Resolution 2.20 Å R-free 0.290 |
| 7ZAU Fascin-1 in complex with Nb 3E11 Deposited 2022-03-22 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
1–493（493 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 5 PEG DI(HYDROXYETHYL)ETHER × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；0.02 M D-glucose, D-mannose, D-galactose, L-fucose, D-xylose, N-acetyl-D-glucosamine; 0.1 M MES/imidazole pH 6.5, 10% PEG 8000, 20% ethylene glycol
|
Resolution 2.20 Å R-free 0.290 |
| 7ZAU Fascin-1 in complex with Nb 3E11 Deposited 2022-03-22 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
1–493（493 aa）
|
Not recorded | EDO 1,2-ETHANEDIOL × 3 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；295 K；0.02 M D-glucose, D-mannose, D-galactose, L-fucose, D-xylose, N-acetyl-D-glucosamine; 0.1 M MES/imidazole pH 6.5, 10% PEG 8000, 20% ethylene glycol
|
Resolution 2.20 Å R-free 0.290 |
| 8VO5 Cryo-EM structure of fascin bound to F-actin (actin binding site 1) Deposited 2024-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8VO6 Cryo-EM structure of fascin bound to F-actin (actin binding site 2) Deposited 2024-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 3 MG MAGNESIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8VO7 Cryo-EM structure of fascin crosslinked F-actin Deposited 2024-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 8VO8 Cryo-EM structure of fascin crosslinked F-actin (Eigen_left) Deposited 2024-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å |
| 8VO9 Cryo-EM structure of fascin crosslinked F-actin (Eigen_middle) Deposited 2024-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 8VOA Cryo-EM structure of fascin crosslinked F-actin (Eigen_right) Deposited 2024-01-14 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ADP ADENOSINE-5'-DIPHOSPHATE × 6 MG MAGNESIUM ION × 6 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.00 Å |
| 9FN6 Full-length crystal structure of human Fascin 1 Deposited 2024-06-09 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0,1M HEPES pH 7.5, 20% polyethylene glycol (PEG) 4000, 2% propanol
|
Resolution 2.20 Å R-free 0.304 |
| 9FN6 Full-length crystal structure of human Fascin 1 Deposited 2024-06-09 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–493（493 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；0,1M HEPES pH 7.5, 20% polyethylene glycol (PEG) 4000, 2% propanol
|
Resolution 2.20 Å R-free 0.304 |
| 9GS6 Crystal structure of fascin in complex with compound 4 Deposited 2024-09-13 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | ACT ACETATE ION × 1 A1IPD N-(1-(3,4-dichlorobenzyl)-6-oxo-1,6-dihydropyridin-3-yl)-5-oxopyrrolidine-2-carboxamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5；292 K；18-22% PEG 8000, 100-130 mM MgAc2, 100 mM citric acid pH 5.0
|
Resolution 1.97 Å R-free 0.268 |
| 9GXI Subtomogram average of fascin-actin complex Deposited 2024-09-30 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain A
1–493（493 aa）
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.00 Å |