当前蛋白身份:Q16820
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差异标签只比较当前检索结果;所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly;同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 4GWM Crystal structure of human promeprin beta 提交 2012-09-03 | Assembly 1 其他组合 同源多聚体;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 A
23–614(592 aa)
片段:Promeprin beta ectomoiety, UNP residues 23-614
链 B
23–614(592 aa)
片段:Promeprin beta ectomoiety, UNP residues 23-614
|
突变:T23P, P24W 突变:T23P, P24W | ZN ZINC ION × 2 NA SODIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 CL CHLORIDE ION × 5 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 9;293 K;18.2%(wt/vol) PEG8000, 1M LiCl, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 1.85 Å R-free 0.188 |
| 4GWN Crystal structure of human mature meprin beta 提交 2012-09-03 | Assembly 1 其他组合 同源多聚体;蛋白 × 2 PDB 声明:dimeric(2) 与蛋白数一致 |
链 A
62–614(553 aa)
片段:Mature meprin beta ectomoiety, UNP residues 62-614
|
未记录 | CD CADMIUM ION × 2 NA SODIUM ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 8.5;293 K;20%(wt/vol) PEG 3350, 0.2M sodium malonate, 0.1M bis-Tris propane, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
分辨率 3.00 Å R-free 0.238 |
| 7AQ1 Crystal structure of human mature meprin beta in complex with the specific inhibitor MWT-S-270 提交 2020-10-20 | Assembly 1 其他组合 同源多聚体;蛋白 × 2 PDB 声明:dimer(2) 与蛋白数一致 |
链 A
62–595(534 aa)
链 B
62–595(534 aa)
|
未记录 | ZN ZINC ION × 2 EDO 1,2-ETHANEDIOL × 5 RUE 3-[[(3-Carboxyphenyl)methyl-[2-(hydroxyamino)-2-oxoethyl]amino]methyl]benzoic acid × 2 CA CALCIUM ION × 4 CL CHLORIDE ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 7.6;286 K;Protein was mixed with inhibitor in a molar ratio of 1 to 1.2 to a final protein concentration of 8 mg/mL in 30 mM Tris, 100 mM sodium chloride, pH 7.6.
200 nL protein/inhibitor was mixed with 200 nL praezipitation buffer and equilibrated against 55 micro Liter of praezipitation buffer, containing 25 percent (w/v) PEG 4000 and 30 percent (v/v) ethylene glycol.
|
分辨率 2.41 Å R-free 0.236 |
| 7AUW Inhibitory complex of human meprin beta with mouse fetuin-B. 提交 2020-11-03 | Assembly 1 其他组合 异源复合物;蛋白 × 4 PDB 声明:tetrameric(4) 与蛋白数一致 |
链 A
62–608(547 aa)
链 C
62–608(547 aa)
|
未记录 | NA SODIUM ION × 2 ZN ZINC ION × 2 SO4 SULFATE ION × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP;pH 9.3;293 K;20.0% PEG smear medium, 0.1M ammonium sulfate, 0.05M magnesium sulfate heptahydrate, 0.1M bicine pH 9.3, 10% ethylene glycol.
|
分辨率 2.80 Å R-free 0.261 |