当前蛋白身份：Q582V7
重新检索
差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 10JU Crystal Structure of serine/threonine-protein kinase (AEK1) T376D, S395D Mutant from Trypanosoma brucei (AMP-PNP) 提交 2026-01-22 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
54–406（353 aa）
片段：residues 54-406
链 B
54–406（353 aa）
片段：residues 54-406
|
突变：T376D, S395D 非标准单体：是（mmCIF未提供具体位点） 突变：T376D, S395D | SO4 SULFATE ION × 11 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.5；291 K；Index F6 0.2M Ammonium sulfate 0.1M Bis-Tris pH 5.5 25% PEG 3350. TrbrA.01480.a.WW4.PS38793 at 13.5 mg/mL. The C-terminal tail ~60 residues was disordered in each subunit. Residue Ser 71 in subunit A contained a large amount of density near the OG atom. This was modeled as a phosphoserine (SEP) although this is not a predicted phosphorylation site. 2mM AMP-PNP and 2mM MgCl2 added prior to crystallization. Prominent AMP-PNP density in subunit B but disorder in the phosphate portion. plate 20522 F6 drop 2, Puck: PSL-0904, Cryo: 80% crystallant + 20% glycerol
|
分辨率 2.15 Å R-free 0.250 |
| 9Z8K Crystal Structure of serine/threonine-protein kinase (AEK1) from Trypanosoma brucei 提交 2025-11-18 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
54–406（353 aa）
片段：residues 54-406
链 B
54–406（353 aa）
片段：residues 54-406
|
非标准单体：是（mmCIF未提供具体位点） | SO4 SULFATE ION × 11 CL CHLORIDE ION × 10 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.5；291 K；IDX G2 (25% (w/v) PEG 3350, 0.1 M BIS-TRIS pH 5.5, 0.2 M lithium sulfate. TrbrA.01480.a.WW4.PS38793 at 13.5 mg/mL. The C-terminal tail ~60 residues was disordered in each subunit. Residue Ser 71 in subunit A contained a large amount of density near the OG atom. This was modeled as a phosphoserine (SEP) although this is not a predicted phosphorylation site. plate 20520 G2 drop 1, Puck: PSL-0604, Cryo: 80% crystallant + 20% glycerol
|
分辨率 2.05 Å R-free 0.235 |