当前蛋白身份：Q5V8Z9
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相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 4WRT Crystal structure of Influenza B polymerase with bound vRNA promoter (form FluB2) 提交 2014-10-25 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 9；281 K；1 M LiCl, 10% PEG 6000 and 0.1 M bicine pH 9.0
|
分辨率 2.70 Å R-free 0.211 |
| 4WSA Crystal structure of Influenza B polymerase bound to the vRNA promoter (FluB1 form) 提交 2014-10-26 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 9；281 K；FluB polymerase was concentrated to 9 mg per ml (37 microM) in a buffer containing 500 mM NaCl, 50 mM Hepes pH 7.5, 5% glycerol and 2mM Tris(2-carboxyethyl)phosphine (TCEP), and mixed with 40 microM vRNA for crystallization in hanging drops at 4 C. A trigonal crystal form (FluB1) was obtained by mixing polymerase with nucleotides 5-18 of the 3 prime end and 1-14 of the 5 prime end of the vRNA in a condition containing 0.1 M bicine pH 9.0, 10% MPD.
|
分辨率 3.40 Å R-free 0.265 |
| 5EPI CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT 提交 2015-11-11 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；100 MM TRI SODIUM CITRATE PH
5.6, 10 OR 100 MM NACL AND 12% MPD
|
分辨率 4.10 Å R-free 0.287 |
| 5EPI CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT 提交 2015-11-11 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 E
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；100 MM TRI SODIUM CITRATE PH
5.6, 10 OR 100 MM NACL AND 12% MPD
|
分辨率 4.10 Å R-free 0.287 |
| 5EPI CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT 提交 2015-11-11 | Assembly 3 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 I
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；100 MM TRI SODIUM CITRATE PH
5.6, 10 OR 100 MM NACL AND 12% MPD
|
分辨率 4.10 Å R-free 0.287 |
| 5EPI CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT 提交 2015-11-11 | Assembly 4 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 M
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；100 MM TRI SODIUM CITRATE PH
5.6, 10 OR 100 MM NACL AND 12% MPD
|
分辨率 4.10 Å R-free 0.287 |
| 5EPI CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT 提交 2015-11-11 | Assembly 5 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 Q
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；100 MM TRI SODIUM CITRATE PH
5.6, 10 OR 100 MM NACL AND 12% MPD
|
分辨率 4.10 Å R-free 0.287 |
| 5EPI CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT 提交 2015-11-11 | Assembly 6 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 U
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；100 MM TRI SODIUM CITRATE PH
5.6, 10 OR 100 MM NACL AND 12% MPD
|
分辨率 4.10 Å R-free 0.287 |
| 5FML Crystal structure of the endonuclease from the PA subunit of influenza B virus bound to the PB2 subunit NLS peptide 提交 2015-11-06 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 B
1–197（197 aa）
片段：ENDONUCLEASE DOMAIN RESIDUES 1-197
|
未记录 | MG MAGNESIUM ION × 2 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 6.5；0.1 M MES PH 6.5, 25% PEG6000
|
分辨率 1.70 Å R-free 0.276 |
| 5FMZ Crystal structure of Influenza B polymerase with bound 5' vRNA 提交 2015-11-10 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 D
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；0.1 M KCL, 10 MM MGCL2, 50 MM TRIS-HCL PH 8.5 AND 30% PEG400
|
分辨率 3.40 Å R-free 0.277 |
| 5FMZ Crystal structure of Influenza B polymerase with bound 5' vRNA 提交 2015-11-10 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 8.5；0.1 M KCL, 10 MM MGCL2, 50 MM TRIS-HCL PH 8.5 AND 30% PEG400
|
分辨率 3.40 Å R-free 0.277 |
| 5M3J Influenza B polymerase bound to four heptad repeats of serine 5 phosphorylated Pol II CTD 提交 2016-10-14 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 4 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 9；277 K；FluB polymerase in a buffer containing 500 mM NaCl, 50 mM Hepes pH 7.5, 5% glycerol and 2mM Tris(2-carboxyethyl)phosphine (TCEP) was mixed with 5' and 3' ends of vRNA promoter and CTD peptide. Reservoir solution contained 0.1 M bicine pH 9.0, 10% MPD.
|
分辨率 3.50 Å R-free 0.268 |
| 5MSG Influenza B polymerase bound to vRNA promoter and capped RNA primer 提交 2017-01-04 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | PO4 PHOSPHATE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4；277 K；35 microM of FluB polymerase was mixed with 40 microM of the vRNA promoter and 40 microM 13-mer capped RNA primer in a buffer containing 500 mM NaCl, 50 mM HEPES pH 7.5, 5% glycerol and 2 mM TCEP. The best diffracting crystals appeared in 100 mM sodium acetate pH 3.8 - 4.0 and 150 mM di-ammonium phosphate
|
分辨率 3.80 Å R-free 0.267 |
| 6F5O A mechanism for the activation of the influenza virus transcriptase 提交 2017-12-02 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：pentameric（5） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 9.80 Å |
| 6FS8 Influenza B/Memphis/13/03 endonuclease with bound inhibitor, baloxavir acid (BXA) 提交 2018-02-19 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–197（197 aa）
|
未记录 | E4Z Baloxavir acid × 1 MN MANGANESE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；Protein, at 15-17 mg/ml, was incubated with 10-fold molar excess of BXA for 30 min at RT, mixtures were centrifuged at RT for 5 minutes at 12000 g, and soluble fraction was used for crystallization trials (final protein concentration 8-10 mg/ml).
Mother liquor was 0.2 M NaCl, 0.1 M Na(CH3)2AsO2 pH 6.5, 2 M (NH4)2SO4
|
分辨率 1.80 Å R-free 0.215 |
| 6FS8 Influenza B/Memphis/13/03 endonuclease with bound inhibitor, baloxavir acid (BXA) 提交 2018-02-19 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–197（197 aa）
|
未记录 | E4Z Baloxavir acid × 1 MN MANGANESE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；Protein, at 15-17 mg/ml, was incubated with 10-fold molar excess of BXA for 30 min at RT, mixtures were centrifuged at RT for 5 minutes at 12000 g, and soluble fraction was used for crystallization trials (final protein concentration 8-10 mg/ml).
Mother liquor was 0.2 M NaCl, 0.1 M Na(CH3)2AsO2 pH 6.5, 2 M (NH4)2SO4
|
分辨率 1.80 Å R-free 0.215 |
| 6FS9 Influenza B/Memphis/13/03 endonuclease with I38T mutation with bound inhibitor, baloxavir acid (BXA) 提交 2018-02-19 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–197（197 aa）
|
突变：I38T | MN MANGANESE (II) ION × 2 E4Z Baloxavir acid × 1 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；Protein, at 15-17 mg/ml, was incubated with 10-fold molar excess of BXA for 30 min at RT, mixtures were centrifuged at RT for 5 minutes at 12000 g, and soluble fraction was used for crystallization trials (final protein concentration 8-10 mg/ml).
Mother liquor was 0.2 M CaCl2, 0.1 M MES pH 6.0, 20% (w/v) PEG6000
|
分辨率 2.28 Å R-free 0.235 |
| 6FSB Influenza B/Memphis/13/03 endonuclease with I38T mutation 提交 2018-02-19 | Assembly 1 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
1–197（197 aa）
|
突变：I38T | MN MANGANESE (II) ION × 2 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；Protein, at 15-17 mg/ml, was incubated with 10-fold molar excess of BXA for 30 min at RT, mixtures were centrifuged at RT for 5 minutes at 12000 g, and soluble fraction was used for crystallization trials (final protein concentration 8-10 mg/ml).
Mother liquor was
|
分辨率 1.80 Å R-free 0.236 |
| 6FSB Influenza B/Memphis/13/03 endonuclease with I38T mutation 提交 2018-02-19 | Assembly 2 蛋白单体 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
1–197（197 aa）
|
突变：I38T | MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；293 K；Protein, at 15-17 mg/ml, was incubated with 10-fold molar excess of BXA for 30 min at RT, mixtures were centrifuged at RT for 5 minutes at 12000 g, and soluble fraction was used for crystallization trials (final protein concentration 8-10 mg/ml).
Mother liquor was
|
分辨率 1.80 Å R-free 0.236 |
| 6QCS Influenza B polymerase pre-initiation complex 提交 2018-12-30 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 6QCT Influenza B polymerase elongation complex 提交 2018-12-30 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 6QCV Crystal structure of influenza B polymerase initiation state with capped 14-mer RNA primer and CTP 提交 2018-12-31 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | PO4 PHOSPHATE ION × 1 CTP CYTIDINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.4；277 K；Influenza B polymerase at 9 mg per ml in was mixed with 40 microM vRNA 5 prime end 14-mer, 40 microM vRNA 3 prime end 21-mer and 80 microM 14-mer capped RNA. The mother liquor contained 200 mM di-ammonium phosphate and 100 mM sodium acetate between pH 4.0 and 4.4. CTP was soaked for 18 hours into grown crystals at a final concentration of 5 mM in the drop.
|
分辨率 3.24 Å R-free 0.234 |
| 6QCW Crystal structure of influenza B polymerase initiation state with capped 14-mer RNA primer 提交 2018-12-31 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | PO4 PHOSPHATE ION × 3 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.4；277 K；Influenza B polymerase at 9 mg per ml in was mixed with 40 microM vRNA 5 prime end 14-mer, 40 microM vRNA 3 prime end 21-mer and 80 microM 14-mer capped RNA. The mother liquor contained 200 mM di-ammonium phosphate and 100 mM sodium acetate between pH 4.0 and 4.4.
|
分辨率 2.88 Å R-free 0.234 |
| 6QCX Crystal structure of influenza B polymerase initiation state with capped 15-mer RNA primer 提交 2018-12-31 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | PO4 PHOSPHATE ION × 1 POP PYROPHOSPHATE 2- × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.4；277 K；Influenza B polymerase at 9 mg per ml in was mixed with 40 microM vRNA 5 prime end 14-mer, 40 microM vRNA 3 prime end 21-mer and 80 microM 15-mer capped RNA. The mother liquor contained 200 mM di-ammonium phosphate and 100 mM sodium acetate between pH 4.0 and 4.4.
|
分辨率 3.08 Å R-free 0.226 |
| 6T0W Human Influenza B polymerase recycling complex 提交 2019-10-03 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.18 Å |
| 7R1F Early transcription elongation state of influenza B polymerase backtracked due to double incoproation of nucleotide analogue T1106 提交 2022-02-02 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 3 GTA P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.58 Å |
| 7Z42 Influenza B polymerase with Pol II pSer5 CTD peptide mimic bound in site 2B 提交 2022-03-03 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 5 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
突变：K135A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；FluPolB PA mutant K135A at 9 mg per ml was mixed with 40 microM of nucleotides 1-13 vRNA 5prime end, 5-pAGUAGUAACAAGA-3, and 1.8 mM 28-mer CTD peptide, YSPTpSPS x 4 in a buffer containing 50 mM HEPES pH 7.5, 500 mM NaCl, 5% glycerol, 2 mM TCEP. Hanging drops for crystallization were set up at 20 C.
|
分辨率 2.42 Å R-free 0.257 |
| 7Z42 Influenza B polymerase with Pol II pSer5 CTD peptide mimic bound in site 2B 提交 2022-03-03 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 5 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 D
1–726（726 aa）
|
突变：K135A | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；FluPolB PA mutant K135A at 9 mg per ml was mixed with 40 microM of nucleotides 1-13 vRNA 5prime end, 5-pAGUAGUAACAAGA-3, and 1.8 mM 28-mer CTD peptide, YSPTpSPS x 4 in a buffer containing 50 mM HEPES pH 7.5, 500 mM NaCl, 5% glycerol, 2 mM TCEP. Hanging drops for crystallization were set up at 20 C.
|
分辨率 2.42 Å R-free 0.257 |
| 7Z43 Influenza B polymerase with Pol II pSer5 CTD peptide mimic bound in site 1B and 2B 提交 2022-03-03 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 5 PDB 声明：octameric（8） 与全部聚合物一致 |
链 AAA
1–726（726 aa）
|
未记录 | PO4 PHOSPHATE ION × 1 IC5 [(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-1~{H}-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphono hydrogen phosphate × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 4.4；277 K；200 mM di-ammonium phosphate, and 100 mM sodium acetate at pH 4.4
|
分辨率 3.12 Å R-free 0.272 |
| 8BDR Early transcription elongation state of influenza B/Mem polymerase backtracked due to double incoproation of nucleotide analogue T1106 and with singly incoporated T1106 at the U +1 position 提交 2022-10-19 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 2 GTA P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.70 Å |
| 8BE0 Early transcription elongation state of influenza B/Mem polymerase backtracked due to double incoproation of nucleotide analogue T1106 and with singly incoporated T1106 at the C +1 position 提交 2022-10-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 2 GTA P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.34 Å |
| 8BF5 Early transcription elongation state of influenza A/H7N9 polymerase stalled with incoming GTP analogue 提交 2022-10-24 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：hexameric（6） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 3 G2P PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 GTA P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.96 Å |
| 8RN1 Influenza B polymerase, monomeric encapsidase with 5' cRNA hook bound 提交 2024-01-09 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 3 PDB 声明：tetrameric（4） 与全部聚合物一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.64 Å |
| 8RN2 Monomeric apo-influenza B polymerase, encapsidase conformation 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.89 Å |
| 8RN3 Pseudo-symmetrical influenza B polymerase apo-dimer, encapsidase moiety (from "Influenza B polymerase pseudo-symmetrical dimer" | Local refinement) 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 A
1–726（726 aa）
链 D
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 3 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.78 Å |
| 8RN4 Pseudo-symmetrical influenza B polymerase apo-dimer, ENDO(T) moiety (from "Influenza B polymerase pseudo-symmetrical dimer" | Local refinement) 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 A
1–726（726 aa）
链 D
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.87 Å |
| 8RN5 Pseudo-symmetrical influenza B polymerase apo-dimer, ENDO(R) moiety (from "Influenza B polymerase pseudo-symmetrical dimer" | Local refinement) 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 A
1–726（726 aa）
链 E
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.88 Å |
| 8RN6 Pseudo-symmetrical influenza B polymerase apo-dimer, ENDO(E) moiety (from "Influenza B polymerase pseudo-symmetrical dimer" | Local refinement) 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 A
1–726（726 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.82 Å |
| 8RN7 Pseudo-symmetrical influenza B polymerase apo-dimer, core-only moeity (from "Influenza B polymerase pseudo-symmetrical dimer" | Local refinement) 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 A
1–726（726 aa）
链 E
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.09 Å |
| 8RN8 Influenza B polymerase pseudo-symmetrical apo-dimer (FluPol(E)|FluPol(S)) 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 6 PDB 声明：hexameric（6） 与蛋白数一致 |
链 A
1–726（726 aa）
链 D
1–726（726 aa）
|
未记录 | MG MAGNESIUM ION × 5 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.92 Å |
| 8RN9 Influenza B polymerase, replicase (from "Influenza B polymerase apo-trimer" | Local refinement) 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 A
1–726（726 aa）
链 E
1–726（726 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.31 Å |
| 8RNA Influenza B polymerase apo-trimer 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 10 PDB 声明：decameric（10） 与蛋白数一致 |
链 A
1–726（726 aa）
链 D
1–726（726 aa）
链 X
1–726（726 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.57 Å |
| 8RNB Influenza B polymerase, encapsidase plus 627(R) / human ANP32A (from "Influenza B polymerase apo-trimer" | Local refinement) 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 5 PDB 声明：pentameric（5） 与蛋白数一致 |
链 D
1–726（726 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.31 Å |
| 8RNC Influenza B polymerase, replication complex, an asymmetric polymerase dimer bound to human ANP32A (from "Influenza B polymerase apo-trimer" | Local refinement) 提交 2024-01-09 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 9 PDB 声明：nonameric（9） 与蛋白数一致 |
链 A
1–726（726 aa）
链 D
1–726（726 aa）
链 X
1–726（726 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 8
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.52 Å |