当前蛋白身份：Q72I28
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 4V4I Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. 提交 2007-02-15 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：54-meric（54） 与全部聚合物一致 |
链 b
1–37（37 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24-26% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
分辨率 3.71 Å R-free 0.353 |
| 4V4J Interactions and Dynamics of the Shine-Dalgarno Helix in the 70S Ribosome. 提交 2007-07-18 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 48 PDB 声明：54-meric（54） 与全部聚合物一致 |
链 b
1–37（37 aa）
|
未记录 | 未记录非水小分子 | X-RAY DIFFRACTION |
X-ray结晶条件
pH 7.5；298 K；Buffer conditions: 25 mM K-HEPES (pH 7.5), 12.5 mM Na-cacodylate pH 5.5, 2.5 mM Tris-HCl (pH 7.5), 80 mM NH_4 Cl, 80 mM KCl, 11 mM MgCl_2, 6.5 mM Thermine HCl and 0.2 M KSCN. Crystallization by the hanging drop method over 26-30% MPD in the above buffer mix, using 2 uL of ribosome complex mixed with 2 uL of well mixture on siliconized cover slips, temperature 298K
|
分辨率 3.83 Å R-free 0.351 |
| 4V68 T. thermophilus 70S ribosome in complex with mRNA, tRNAs and EF-Tu.GDP.kirromycin ternary complex, fitted to a 6.4 A Cryo-EM map. 提交 2008-12-11 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 52 PDB 声明：60-meric（60） 与全部聚合物一致 |
链 B9
2–36（35 aa）
|
未记录 | PHA PHENYLALANINAL × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MAU N-METHYL KIRROMYCIN × 1 BME BETA-MERCAPTOETHANOL × 1 | ELECTRON MICROSCOPY |
cryo-EM玻璃化条件
冷冻剂 METHANE；A Vitrobot was used to shock-freeze the sample in liquid methane.
|
分辨率 6.40 Å |
| 4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 B9
1–37（37 aa）
|
未记录 | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
|
分辨率 3.86 Å R-free 0.317 |
| 4V9J 70S ribosome translocation intermediate GDPNP-II containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-23 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 D9
1–37（37 aa）
|
未记录 | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG20000, 2.8% PPG P400, 5.1% PEG550 MME, 200 mM potassium thiocyanate, Tris acetate
|
分辨率 3.86 Å R-free 0.317 |
| 4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-24 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 B9
1–37（37 aa）
|
未记录 | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 3.50 Å R-free 0.309 |
| 4V9K 70S ribosome translocation intermediate GDPNP-I containing elongation factor EFG/GDPNP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-24 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 D9
1–37（37 aa）
|
未记录 | GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 3.50 Å R-free 0.309 |
| 4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-24 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 B9
1–37（37 aa）
|
未记录 | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 3.50 Å R-free 0.328 |
| 4V9L 70S Ribosome translocation intermediate FA-3.6A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-24 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 56 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 D9
1–37（37 aa）
|
未记录 | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 3.50 Å R-free 0.328 |
| 4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-25 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 55 PDB 声明：60-meric（60） 与全部聚合物一致 |
链 B9
1–37（37 aa）
|
未记录 | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 4.00 Å R-free 0.329 |
| 4V9M 70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state. 提交 2013-04-25 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 55 PDB 声明：60-meric（60） 与全部聚合物一致 |
链 D9
1–37（37 aa）
|
未记录 | FUA FUSIDIC ACID × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
|
分辨率 4.00 Å R-free 0.329 |
| 4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. 提交 2014-07-02 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 55 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 B9
1–37（37 aa）
|
未记录 | GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 4 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.80 Å R-free 0.331 |
| 4W29 70S ribosome translocation intermediate containing elongation factor EFG/GDP/fusidic acid, mRNA, and tRNAs trapped in the AP/AP pe/E chimeric hybrid state. 提交 2014-07-02 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 55 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 D9
1–37（37 aa）
|
未记录 | GDP GUANOSINE-5'-DIPHOSPHATE × 1 FUA FUSIDIC ACID × 1 NMY NEOMYCIN × 2 MG MAGNESIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.80 Å R-free 0.331 |
| 4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome 提交 2015-04-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55MERIC（55） 与全部聚合物一致 |
链 19
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 1359 UNX UNKNOWN LIGAND × 1 MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 2 ARG ARGININE × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
分辨率 3.10 Å R-free 0.271 |
| 4ZER Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome 提交 2015-04-20 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55MERIC（55） 与全部聚合物一致 |
链 29
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 943 UNX UNKNOWN LIGAND × 1 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 K POTASSIUM ION × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 7.6；293 K；Tris-HCl, PEG 20000, MPD, Arginine
|
分辨率 3.10 Å R-free 0.271 |
| 5A9Z Complex of Thermous thermophilus ribosome bound to BipA-GDPCP 提交 2015-07-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 52 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 Af
1–37（37 aa）
|
未记录 | NMY NEOMYCIN × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM玻璃化条件
冷冻剂 ETHANE；LIQUID ETHANE
|
分辨率 4.70 Å |
| 5AA0 Complex of Thermous thermophilus ribosome (A-and P-site tRNA) bound to BipA-GDPCP 提交 2015-07-23 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 52 PDB 声明：58-meric（58） 与全部聚合物一致 |
链 Af
1–37（37 aa）
|
未记录 | NMY NEOMYCIN × 2 8AN 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) × 2 GCP PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL；pH 7.5；5 MM HEPES PH 7.5, 10 MM MGAC, 50 MM KCL, 10 MM NH4CL, AND 6 MM 2- MERCAPTOETHANOL
cryo-EM玻璃化条件
冷冻剂 ETHANE；LIQUID ETHANE
|
分辨率 5.00 Å |
| 5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon 提交 2016-03-31 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 GA
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 1510 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
|
分辨率 3.10 Å R-free 0.256 |
| 5J4D E. coli release factor 1 bound to the 70S ribosome in response to a pseudouridylated stop codon 提交 2016-03-31 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 LC
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 1383 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3.1 uL 70S*mRNA*tRNAfMet complex + 3.1 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate), reservoir: 300 uL 0.5-0.7 M sodium chloride, 4-fold molar excess of E. coli release factor 1 added during complex formation
|
分辨率 3.10 Å R-free 0.256 |
| 5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 提交 2017-03-22 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：52-meric（52） 与全部聚合物一致 |
链 19
1–37（37 aa）
|
未记录 | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 4 MG MAGNESIUM ION × 1306 ARG ARGININE × 2 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
|
分辨率 3.25 Å R-free 0.270 |
| 5V8I Thermus thermophilus 70S ribosome lacking ribosomal protein uS17 提交 2017-03-22 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 49 PDB 声明：52-meric（52） 与全部聚合物一致 |
链 29
1–37（37 aa）
|
未记录 | MPD (4S)-2-METHYL-2,4-PENTANEDIOL × 3 MG MAGNESIUM ION × 870 ZN ZINC ION × 6 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION；pH 7.6；293 K；0.1 M Tris-HCl, 0.2 M arginine, 2.75% PEG20,000, 9% 2-methyl-pentanediol, 0.5 mM beta-mercaptoethanol
|
分辨率 3.25 Å R-free 0.270 |
| 6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome 提交 2017-09-27 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 GA
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 896 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.40 Å |
| 6B4V Antibiotic blasticidin S and E. coli release factor 1 bound to the 70S ribosome 提交 2017-09-27 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 KC
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 787 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.40 Å |
| 6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome 提交 2017-11-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 51 PDB 声明：57-meric（57） 与全部聚合物一致 |
链 GA
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 727 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.40 Å |
| 6BOH Antibiotic blasticidin S and E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome 提交 2017-11-20 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 51 PDB 声明：57-meric（57） 与全部聚合物一致 |
链 LC
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 701 BLS BLASTICIDIN S × 1 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1*antibiotic complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 and 1 mM blasticidin S were added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.40 Å |
| 6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome 提交 2017-11-20 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 GA
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 826 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.55 Å |
| 6BOK E. coli release factor 1 (containing deletion 302-304) bound to the 70S ribosome 提交 2017-11-20 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 JC
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 692 ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；3 uL of 70S*mRNA*tRNAfMet*RF1 complex + 3 uL crystallization buffer (0.1 M Tris-HCl, pH 7.5, 4% v/v PEG20000, 8% v/v MPD, 0.2 M potassium thiocyanate). 4-fold molar excess of E. coli release factor 1 was added during complex formation. Reservoir: 300 uL 0.4-0.6 M sodium chloride.
|
分辨率 3.55 Å |
| 6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State 提交 2018-11-08 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：55-meric（55） 与全部聚合物一致 |
链 AL36
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 141 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.20 Å R-free 0.321 |
| 6N1D X-ray Crystal complex showing Spontaneous Ribosomal Translocation of mRNA and tRNAs into a Chimeric Hybrid State 提交 2018-11-08 | Assembly 2 蛋白–RNA 异源复合物；蛋白 × 50 PDB 声明：56-meric（56） 与全部聚合物一致 |
链 BL36
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 218 SF4 IRON/SULFUR CLUSTER × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
LIQUID DIFFUSION；pH 7；295 K；pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 295K
|
分辨率 3.20 Å R-free 0.321 |
| 6Q95 Structure of tmRNA SmpB bound in A site of T. thermophilus 70S ribosome 提交 2018-12-17 | Assembly 1 蛋白–RNA 异源复合物；蛋白 × 54 PDB 声明：61-meric（61） 与全部聚合物一致 |
链 f
1–37（37 aa）
|
未记录 | MG MAGNESIUM ION × 637 ZN ZINC ION × 3 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.5
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.70 Å |