Current Protein Identity:Q8N0V3 New Search
Main Difference Dimensions in This Set
Different construct Different mutation/modification Different assembly state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics

Difference tags compare only the current result set; every original PDB and assembly record remains separate.

Related-Structure Differences

Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.

PDB Entry Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Experimental Method Experimental Conditions Structure Quality
2E7G Solution structure of putative ribosome-binding factor A (RbfA) from human mutochondrial precursor Deposited 2007-01-09 Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count
Chain A 86–201(116 aa) Fragment:KH FOLD
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7;298 K;Ionic strength (raw mmCIF value) 120mM;Pressure ambient
NMR sample composition 0.79mM 13C-15N PROTEIN; 20mM d-Tris-HCl(ph7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O | 90% H2O/10% D2O
Resolution not provided
7PNX Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation a Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 64 K POTASSIUM ION × 16 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.76 Å
7PNY Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation b Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 61 K POTASSIUM ION × 20 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.06 Å
7PNZ Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and METTL15 conformation c Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 61 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.09 Å
7PO0 Assembly intermediate of human mitochondrial ribosome small subunit without mS37 in complex with RBFA and IF3 Deposited 2021-09-08 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 MG MAGNESIUM ION × 65 K POTASSIUM ION × 17 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.90 Å
7X9U Type-II KH motif of human mitochondrial RbfA Deposited 2022-03-16 Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count
Chain A 86–201(116 aa)
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 7;298 K;Ionic strength (raw mmCIF value) 100;Pressure 1
NMR sample composition 0.79 mM [U-99% 13C; U-99% 15N] human mitochondrial RbfA, 100 mM non NaCl, 1 mM [U-99% 2H] d-DTT, 20 mM [U-99% 2H] d-Tris HCl, 0.02 % w/v non NaN3, 90% H2O/10% D2O | 90% H2O/10% D2O
Resolution not provided
8CSR Human mitochondrial small subunit assembly intermediate (State C) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer;Protein × 33 PDB declaration: 34-meric(34) Consistent with all polymers
Chain 6 1–343(343 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 5 MG MAGNESIUM ION × 31 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.54 Å
8CSS Human mitochondrial small subunit assembly intermediate (State D) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer;Protein × 32 PDB declaration: 33-meric(33) Consistent with all polymers
Chain 6 1–343(343 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 7 MG MAGNESIUM ION × 32 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.36 Å
8CST Human mitochondrial small subunit assembly intermediate (State E) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain 6 1–343(343 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 4 MG MAGNESIUM ION × 30 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 2.85 Å
8CSU Human mitochondrial small subunit assembly intermediate (State C*) Deposited 2022-05-13 Assembly 1 Protein–RNA Heteromer;Protein × 32 PDB declaration: 33-meric(33) Consistent with all polymers
Chain 6 1–343(343 aa)
Not recorded SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 5 MG MAGNESIUM ION × 26 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.03 Å
8IPL The structure of human mitochondrial methyltransferase METTL15 with RBFA and SAM Deposited 2023-03-14 Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count
Chain B 227–260(34 aa)
Not recorded SAM S-ADENOSYLMETHIONINE × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;289 K;17% w/v PEG 4000, 0.05M Potassium chloride, 0.1M Lithium chloride, 0.012M Spermine tetrahydrochloride, 0.05M MES PH6.5
Resolution 2.20 Å R-free 0.235
8IPM The structure of human mitochondrial methyltransferase METTL15 with h44_RNA, RBFA and SAM Deposited 2023-03-14 Assembly 1 Protein–RNA Heteromer;Protein × 2 PDB declaration: trimeric(3) Consistent with all polymers
Chain C 227–260(34 aa)
Not recorded SFG SINEFUNGIN × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP;289 K;20% PEG 4000, 0.2 M ADA (pH 6.4)
Resolution 3.10 Å R-free 0.288
9G5B Assembly intermediate of human mitochondrial ribosome small subunit (State A) Deposited 2024-07-16 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded MG MAGNESIUM ION × 12 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.20 Å
9G5C Assembly intermediate of human mitochondrial ribosome small subunit (State B) Deposited 2024-07-16 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded MG MAGNESIUM ION × 23 K POTASSIUM ION × 4 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9G5D Assembly intermediate of human mitochondrial ribosome small subunit (State C) Deposited 2024-07-16 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded MG MAGNESIUM ION × 10 K POTASSIUM ION × 1 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.4;20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9H51 Assembly intermediate of human mitochondrial ribosome small subunit in complex with PUS1, METTL15 and RBFA(IN conformation) (state M1) Deposited 2024-10-22 Assembly 1 Protein–RNA Heteromer;Protein × 33 PDB declaration: 34-meric(34) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 MG MAGNESIUM ION × 40 K POTASSIUM ION × 6 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 SAH S-ADENOSYL-L-HOMOCYSTEINE × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.10 Å
9H54 Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and partial RBFA (state N2) Deposited 2024-10-22 Assembly 1 Protein–RNA Heteromer;Protein × 30 PDB declaration: 31-meric(31) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded MG MAGNESIUM ION × 42 K POTASSIUM ION × 11 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.00 Å
9H55 Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and TFB1M (state N3) Deposited 2024-10-22 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain a 1–343(343 aa)
Not recorded MG MAGNESIUM ION × 41 K POTASSIUM ION × 10 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.40 Å
9IGT Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15, RBFA, and mtIF2 (State M2.1) Deposited 2025-02-20 Assembly 1 Protein–RNA Heteromer;Protein × 33 PDB declaration: 34-meric(34) Consistent with all polymers
Chain a 42–343(302 aa)
Not recorded MG MAGNESIUM ION × 12 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.96 Å
9IGU Assembly intermediate of human mitochondrial ribosome small subunit bound to METTL15 and RBFA (Inward conformation) (State M2) Deposited 2025-02-20 Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers
Chain a 42–343(302 aa)
Not recorded MG MAGNESIUM ION × 31 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 SPM SPERMINE × 1 ELECTRON MICROSCOPY
cryo-EM buffer pH 7.5
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 3.64 Å