当前蛋白身份：Q8NFZ4
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 5XEQ Crystal Structure of human MDGA1 and human neuroligin-2 complex 提交 2017-04-05 | Assembly 1 其他组合 异源复合物；蛋白 × 4 PDB 声明：tetrameric（4） 与蛋白数一致 |
链 A
42–611（570 aa）
片段：UNP residues 42-610
|
突变：N98Q | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；300mM Ammonium citrate tribasic, pH 7.0, 13% 1,3-Butanediol and 18%(v/v) PEG3350
|
分辨率 3.14 Å R-free 0.278 |
| 8GS4 Cryo-EM structure of human Neuroligin 2 提交 2022-09-04 | Assembly 1 蛋白同源多聚体 同源多聚体；蛋白 × 2 PDB 声明：dimeric（2） 与蛋白数一致 |
链 A
1–835（835 aa）
链 B
1–835（835 aa）
|
未记录 | 未记录非水小分子 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 9FAJ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38, in a desensitised state (StateD1) 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 9 PDB 声明：nonameric（9） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 12 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 4 R16 HEXADECANE × 4 HEX HEXANE × 7 PLM PALMITIC ACID × 5 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.60 Å |
| 9FAK CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD2) 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 9 PDB 声明：nonameric（9） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | HEX HEXANE × 6 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 D10 DECANE × 7 PLM PALMITIC ACID × 8 CL CHLORIDE ION × 4 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.60 Å |
| 9FAM CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD3) 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 3 HEX HEXANE × 7 D10 DECANE × 1 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.50 Å |
| 9FAN CryoEM structure of gamma2 subunit of GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 from desensitised state obtained by focused refinement 提交 2024-05-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 CL CHLORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.90 Å |
| 9FAP CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC1) 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 PLM PALMITIC ACID × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.80 Å |
| 9FAQ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC2) 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PLM PALMITIC ACID × 5 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.90 Å |
| 9FAR CryoEM structure of gamma2 subunit of GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 from a closed state, obtained by focused refinement 提交 2024-05-10 | Assembly 1 蛋白异源复合物 异源复合物；蛋白 × 3 PDB 声明：trimeric（3） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 CL CHLORIDE ION × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.90 Å |
| 9FAT CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, Megabody38 and pregnenolone sulfate 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 8 PDB 声明：octameric（8） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 3 CL CHLORIDE ION × 2 R16 HEXADECANE × 1 HEX HEXANE × 3 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 A8W Pregnenolone sulfate × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.60 Å |
| 9FAU CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC1) 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 10 PDB 声明：decameric（10） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | HEX HEXANE × 6 D10 DECANE × 6 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 3 PLM PALMITIC ACID × 2 CLR CHOLESTEROL × 2 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.10 Å |
| 9FAV CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC2) 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 10 PDB 声明：decameric（10） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | HEX HEXANE × 5 D10 DECANE × 4 R16 HEXADECANE × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 3.20 Å |
| 9FAW CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, HEPES and Megabody25, in a desensitised state 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 10 PDB 声明：decameric（10） 与蛋白数一致 |
链 H
668–700（33 aa）
|
未记录 | HEX HEXANE × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 CLR CHOLESTEROL × 1 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.90 Å |
| 9FAX CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with Megabody25, doubly occupied GARLH4 and Neuroligin2 TMD, in a closed state 提交 2024-05-10 | Assembly 1 其他组合 异源复合物；蛋白 × 10 PDB 声明：decameric（10） 与蛋白数一致 |
链 G
668–700（33 aa）
链 H
668–700（33 aa）
|
未记录 | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 R16 HEXADECANE × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 CLR CHOLESTEROL × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 HEX HEXANE × 13 D10 DECANE × 5 PLM PALMITIC ACID × 5 | ELECTRON MICROSCOPY |
cryo-EM缓冲液
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM玻璃化条件
冷冻剂 ETHANE
|
分辨率 2.90 Å |