Isoform 2 of Gamma-aminobutyric acid receptor subunit gamma-2
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 3 PDB declaration: trimeric（3） Consistent with protein copy count | Chain C； UniProt 408–467 | Non-standard monomer：Yes (specific site not provided by mmCIF) | Neuroligin-2 × 1 （Q8NFZ4） LHFPL tetraspan subfamily member 4 protein × 1 （Q7Z7J7） PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 CL CHLORIDE ION × 1 | ELECTRON MICROSCOPY cryo-EM buffer：pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl cryo-EM vitrification conditions：Cryogen ETHANE | Resolution 2.90 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 9FAN | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 6D6U Human GABA-A receptor alpha1-beta2-gamma2 subtype in complex with GABA and flumazenil, conformation A Deposited 2018-04-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
Chain E
439–467（29 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 Y01 CHOLESTEROL HEMISUCCINATE × 12 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3 seconds blot time
|
Resolution 3.92 Å |
| 6HUG CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with picrotoxin and megabody Mb38. Deposited 2018-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 6HUJ CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with picrotoxin, GABA and megabody Mb38. Deposited 2018-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 6HUK CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with bicuculline and megabody Mb38. Deposited 2018-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | H0Z bicuculline methochloride × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.69 Å |
| 6HUO CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with alprazolam (Xanax), GABA and megabody Mb38. Deposited 2018-10-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 08H 8-chloro-1-methyl-6-phenyl-4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.26 Å |
| 6HUP CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with diazepam (Valium), GABA and megabody Mb38. Deposited 2018-10-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 DZP 7-CHLORO-1-METHYL-5-PHENYL-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.58 Å |
| 6I53 Cryo-EM structure of the human synaptic alpha1-beta3-gamma2 GABAA receptor in complex with Megabody38 in a lipid nanodisc Deposited 2018-11-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 6X3S Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with bicuculline methbromide Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
42–361（320 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 J94 (5S)-6,6-dimethyl-5-[(6R)-8-oxo-6,8-dihydrofuro[3,4-e][1,3]benzodioxol-6-yl]-5,6,7,8-tetrahydro[1,3]dioxolo[4,5-g]isoquinolin-6-ium × 2 MAN alpha-D-mannopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 3.12 Å |
| 6X3T Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus propofol Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
42–361（320 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PFL 2,6-BIS(1-METHYLETHYL)PHENOL × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 2.55 Å |
| 6X3U Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus flumazenil Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
42–361（320 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 FYP ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 3.50 Å |
| 6X3V Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus etomidate Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
42–361（320 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 3.50 Å |
| 6X3W Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus phenobarbital Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
42–361（320 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 UQA 5-ethyl-5-phenylpyrimidine-2,4,6(1H,3H,5H)-trione × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 3.30 Å |
| 6X3X Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus diazepam Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
42–361（320 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 DZP 7-CHLORO-1-METHYL-5-PHENYL-1,3-DIHYDRO-2H-1,4-BENZODIAZEPIN-2-ONE × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 2.92 Å |
| 6X3Z Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
42–361（320 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 3.23 Å |
| 6X40 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus picrotoxin Deposited 2020-05-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
42–361（320 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 RI5 (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 2.86 Å |
| 7T0W Complex of GABA-A synaptic receptor with autoimmune antibody Fab115 Deposited 2021-11-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
Chain E
440–467（28 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 7T0Z Complex of GABA-A synaptic receptor with autoimmune antibody Fab175 Deposited 2021-11-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain E
40–361（322 aa）
Chain E
440–467（28 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 MAN alpha-D-mannopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8BHG GABA-A receptor a5 heteromer - a5V2 - Bretazenil Deposited 2022-10-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric |
Chain B
40–409（370 aa）
|
Not recorded | QMU Bretazenil × 5 DMU DECYL-BETA-D-MALTOPYRANOSIDE × 5 NO3 NITRATE ION × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；279 K；22% PEG 400
0.37M potassium nitrate
0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.5
|
Resolution 2.39 Å R-free 0.283 |
| 8DD2 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus Zolpidem Deposited 2022-06-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 R5R Zolpidem × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 2.90 Å |
| 8DD3 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus DMCM Deposited 2022-06-17 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R63 methyl 4-ethyl-6,7-dimethoxy-9H-pyrido[3,4-b]indole-3-carboxylate × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 second blot
|
Resolution 2.90 Å |
| 8SGO Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus pregnenolone sulfate Deposited 2023-04-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
Chain E
439–467（29 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 PTY PHOSPHATIDYLETHANOLAMINE × 6 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 1 A8W Pregnenolone sulfate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.65 Å |
| 8SI9 Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus allopregnanolone Deposited 2023-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
Chain E
439–467（29 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 Y4B allopregnanolone × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.98 Å |
| 8SID Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus dehydroepiandrosterone sulfate Deposited 2023-04-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
Chain E
439–467（29 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 ZWY 17-oxoandrost-5-en-3beta-yl hydrogen sulfate × 1 PTY PHOSPHATIDYLETHANOLAMINE × 6 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 1 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.71 Å |
| 8VQY Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus methaqualone Deposited 2024-01-19 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 A1ADG 2-methyl-3-(2-methylphenyl)quinazolin-4(3H)-one × 3 PTY PHOSPHATIDYLETHANOLAMINE × 4 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.82 Å |
| 8VRN Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus PPTQ Deposited 2024-01-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 A1ADN 3-(4-methylphenyl)-2-phenylquinazolin-4(3H)-one × 2 PTY PHOSPHATIDYLETHANOLAMINE × 5 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 Q3G O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.57 Å |
| 9CRS Native human GABAA receptor of beta2-alpha1-beta2-alpha1-gamma2 assembly Deposited 2024-07-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–475（436 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5s blot
|
Resolution 2.90 Å |
| 9CRV Native human GABAA receptor of beta2-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
40–475（436 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5s blot
|
Resolution 3.18 Å |
| 9CSB Native human GABAA receptor of beta3-alpha1-beta2-alpha2-gamma2 assembly Deposited 2024-07-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain E
40–475（436 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.34 Å |
| 9CT0 Native human GABAA receptor of beta2-alpha1-beta2-alpha2-gamma2 assembly Deposited 2024-07-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain E
40–475（436 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.19 Å |
| 9CTJ Native human GABAA receptor of beta2-alpha1-beta3-alpha2-gamma2 assembly Deposited 2024-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain E
40–475（436 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.74 Å |
| 9CTP Native human GABAA receptor of beta2-alpha1-beta2-alpha3-gamma2 assembly Deposited 2024-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain E
40–475（436 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 3 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.62 Å |
| 9CTV Native human GABAA receptor of beta2-alpha1-gamma2-beta1-alpha2 assembly Deposited 2024-07-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
40–475（436 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.36 Å |
| 9CX7 Native human GABAA receptor of beta3-alpha1-gamma2-beta3-alpha2 assembly Deposited 2024-07-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
40–475（436 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.30 Å |
| 9CXA Native human GABAA receptor of beta2-alpha1-beta3-alpha1-gamma2 assembly Deposited 2024-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
1–475（475 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.04 Å |
| 9CXB Native human GABAA receptor of beta2-alpha1-beta1-alpha2-gamma2 assembly Deposited 2024-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain E
40–475（436 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.33 Å |
| 9CXC Native human GABAA receptor of beta3-alpha1-gamma2-beta2-alpha2 assembly Deposited 2024-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
40–475（436 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.30 Å |
| 9CXD Native human GABAA receptor of beta2-alpha1-beta1-beta1-gamma2 assembly Deposited 2024-07-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain E
40–475（436 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 4 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 3.36 Å |
| 9DRX Human GABAA receptor of beta2-alpha1-beta2-alpha1-gamma2 subtype in complex with GABA plus Lamotrigine Deposited 2024-09-26 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain E
40–361（322 aa）
Chain E
448–475（28 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 IYJ (6M)-6-(2,3-dichlorophenyl)-1,2,4-triazine-3,5-diamine × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE；3.5 s Blot.
|
Resolution 2.95 Å |
| 9EQG CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with GABA and puerarin Deposited 2024-03-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
1–475（475 aa）
|
Not recorded | PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 D10 DECANE × 11 HEX HEXANE × 10 PT5 [(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate × 2 R16 HEXADECANE × 5 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 A1H6W Puerarin × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 100 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.40 Å |
| 9FAJ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38, in a desensitised state (StateD1) Deposited 2024-05-10 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain C
64–467（404 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 12 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 4 R16 HEXADECANE × 4 HEX HEXANE × 7 PLM PALMITIC ACID × 5 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FAK CryoEM structure of human full-length alpha1beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD2) Deposited 2024-05-10 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 9 PDB declaration: nonameric |
Chain C
64–467（404 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | HEX HEXANE × 6 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 5 D10 DECANE × 7 PLM PALMITIC ACID × 8 CL CHLORIDE ION × 4 ABU GAMMA-AMINO-BUTANOIC ACID × 2 R16 HEXADECANE × 3 OLC (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FAM CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, GABA and Megabody38 in a desensitised state (StateD3) Deposited 2024-05-10 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
64–467（404 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 CL CHLORIDE ION × 3 HEX HEXANE × 7 D10 DECANE × 1 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å |
| 9FAP CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC1) Deposited 2024-05-10 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
66–467（402 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 PLM PALMITIC ACID × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FAQ CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 and Megabody38 in a closed state (StateC2) Deposited 2024-05-10 | Different construct Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
66–467（402 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 5 D10 DECANE × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PLM PALMITIC ACID × 5 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 HEX HEXANE × 2 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FAR CryoEM structure of gamma2 subunit of GABA(A)R in complex with GARLH4, the TMD of Neuroligin2 from a closed state, obtained by focused refinement Deposited 2024-05-10 | Parsed fields agree | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric |
Chain C
408–467（60 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 D10 DECANE × 1 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FAS CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with pregnenolone sulfate Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
64–475（412 aa）
|
Not recorded | HEX HEXANE × 8 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 17 PLM PALMITIC ACID × 3 CL CHLORIDE ION × 3 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 R16 HEXADECANE × 5 A8W Pregnenolone sulfate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å |
| 9FAT CryoEM structure of human full-length alpha1beta3gamma2 GABA(A)R in complex with GARLH4, the TMD of Neuroligin2, Megabody38 and pregnenolone sulfate Deposited 2024-05-10 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 8 PDB declaration: octameric |
Chain C
64–467（404 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 3 D10 DECANE × 3 CL CHLORIDE ION × 2 R16 HEXADECANE × 1 HEX HEXANE × 3 PLM PALMITIC ACID × 1 CLR CHOLESTEROL × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 A8W Pregnenolone sulfate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9FAU CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC1) Deposited 2024-05-10 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
65–467（403 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | HEX HEXANE × 6 D10 DECANE × 6 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 2 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 3 PLM PALMITIC ACID × 2 CLR CHOLESTEROL × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FAV CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2 and Megabody25, in a closed state (StateC2) Deposited 2024-05-10 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
64–467（404 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | HEX HEXANE × 5 D10 DECANE × 4 R16 HEXADECANE × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9FAW CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with GARLH4, the TMD of Neuroligin2, HEPES and Megabody25, in a desensitised state Deposited 2024-05-10 | Different construct Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
60–467（408 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | HEX HEXANE × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 3 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FAX CryoEM structure of human full-length beta3gamma2 GABA(A) receptor in complex with Megabody25, doubly occupied GARLH4 and Neuroligin2 TMD, in a closed state Deposited 2024-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Other combination Heteromer；Protein × 10 PDB declaration: decameric |
Chain C
64–467（404 aa）
Chain E
64–467（404 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 2 R16 HEXADECANE × 5 PX2 1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE × 2 CLR CHOLESTEROL × 2 PGW (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate × 4 HEX HEXANE × 13 D10 DECANE × 5 PLM PALMITIC ACID × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；15 mM Hepes pH 7.4, 150 mM NaCl
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FFV Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with Nb38 in the long-lived symmetric resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
40–361（322 aa）
Chain C
447–475（29 aa）
|
Not recorded | D10 DECANE × 5 CL CHLORIDE ION × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å |
| 9FFW Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
40–361（322 aa）
Chain C
447–475（29 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FFX Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
40–361（322 aa）
Chain C
447–475（29 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9FFY Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open1 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
40–361（322 aa）
Chain C
447–475（29 aa）
|
Not recorded | D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FFZ Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 1 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
40–361（322 aa）
Chain C
447–475（29 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 1 CLR CHOLESTEROL × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FG0 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric open 2 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
64–361（298 aa）
Chain C
447–475（29 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å |
| 9FG1 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the short-lived asymmetric desensitised 2 state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
40–361（322 aa）
Chain C
447–475（29 aa）
|
Not recorded | ABU GAMMA-AMINO-BUTANOIC ACID × 2 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 D10 DECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FG2 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
40–361（322 aa）
Chain C
447–475（29 aa）
|
Not recorded | D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 CL CHLORIDE ION × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 5 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 9FG3 Cryo-EM structure of the alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Nb38 bound twice in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 7 PDB declaration: heptameric |
Chain C
40–361（322 aa）
Chain C
447–475（29 aa）
|
Not recorded | D10 DECANE × 5 D3D (19S,22R,25R)-22,25,26-trihydroxy-16,22-dioxo-17,21,23-trioxa-22lambda~5~-phosphahexacosan-19-yl (9E)-octadec-9-enoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å |
| 9FG7 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the short-lived symmetric bound-closed state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
1–475（475 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 D10 DECANE × 3 CL CHLORIDE ION × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9FG8 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
1–475（475 aa）
|
Not recorded | CL CHLORIDE ION × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 ABU GAMMA-AMINO-BUTANOIC ACID × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FG9 Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in complex with GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
1–475（475 aa）
|
Not recorded | CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 LBN 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine × 2 R16 HEXADECANE × 2 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å |
| 9FGA Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to two PIP2 molecules and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 3 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FGB Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-A and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å |
| 9FGC Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs bound to one PIP2 molecule at chain alpha1-D and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | D10 DECANE × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |
| 9FGD Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in SMALPs without PIP2 and in complex with Mb38 Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 D10 DECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å |
| 9FGF Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc in the long-lived symmetric resting state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions | Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric |
Chain C
1–475（475 aa）
|
Not recorded | CL CHLORIDE ION × 3 PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 R16 HEXADECANE × 2 D10 DECANE × 5 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å |
| 9FGG Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in Saposin A nanodisc bound to GABA and Etomidate in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 D10 DECANE × 5 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 V8D Etomidate × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 R16 HEXADECANE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å |
| 9FGH Cryo-EM structure of the full-length alpha1beta3gamma2 GABA(A) receptor in large MSP2N2 nanodisc in complex with GABA in the long-lived symmetric desensitised state Deposited 2024-05-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Other combination Heteromer；Protein × 6 PDB declaration: hexameric |
Chain C
1–475（475 aa）
|
Not recorded | PIO [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate × 2 CL CHLORIDE ION × 3 ABU GAMMA-AMINO-BUTANOIC ACID × 2 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
70 other PDB entries and 70 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | GBRG2_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain C； PDBConstruct 1–60； UniProt 408–467 |
