Current Protein Identity:Q8NG31
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 3SI5 Kinetochore-BUBR1 kinase complex Deposited 2011-06-17 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain X
234–252(19 aa)
Fragment:UNP residues 234-252
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6;292 K;15% PEG 6000, 0.1M Magnesium Acetate, 0.1M Sodium Cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.20 Å R-free 0.247 |
| 3SI5 Kinetochore-BUBR1 kinase complex Deposited 2011-06-17 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain Y
234–252(19 aa)
Fragment:UNP residues 234-252
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 6;292 K;15% PEG 6000, 0.1M Magnesium Acetate, 0.1M Sodium Cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K
|
Resolution 2.20 Å R-free 0.247 |
| 4A1G The crystal structure of the human Bub1 TPR domain in complex with the KI motif of Knl1 Deposited 2011-09-15 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain E
150–200(51 aa)
Fragment:KI MOTIF, RESIDUES 150-200
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6;1.25-1.3 M NA MALONATE, PH 6.0.
|
Resolution 2.60 Å R-free 0.244 |
| 4A1G The crystal structure of the human Bub1 TPR domain in complex with the KI motif of Knl1 Deposited 2011-09-15 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain F
150–200(51 aa)
Fragment:KI MOTIF, RESIDUES 150-200
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6;1.25-1.3 M NA MALONATE, PH 6.0.
|
Resolution 2.60 Å R-free 0.244 |
| 4A1G The crystal structure of the human Bub1 TPR domain in complex with the KI motif of Knl1 Deposited 2011-09-15 | Assembly 3 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain G
150–200(51 aa)
Fragment:KI MOTIF, RESIDUES 150-200
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6;1.25-1.3 M NA MALONATE, PH 6.0.
|
Resolution 2.60 Å R-free 0.244 |
| 4A1G The crystal structure of the human Bub1 TPR domain in complex with the KI motif of Knl1 Deposited 2011-09-15 | Assembly 4 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain H
150–200(51 aa)
Fragment:KI MOTIF, RESIDUES 150-200
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
pH 6;1.25-1.3 M NA MALONATE, PH 6.0.
|
Resolution 2.60 Å R-free 0.244 |
| 4NF9 Structure of the Knl1/Nsl1 complex Deposited 2013-10-31 | Assembly 1 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain A
2117–2337(221 aa)
Fragment:Knl1 C-terminal domain, UNP residues 2117-2337
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;293 K;16-20 % (v/v) PEG 3350, 220 mM sodium citrate and 100 mM MES (pH 6 or 6.5) and 100 mM Hepes (pH 7), VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.265 |
| 4NF9 Structure of the Knl1/Nsl1 complex Deposited 2013-10-31 | Assembly 2 Protein heterocomplex Heteromer;Protein × 2 PDB declaration: dimeric(2) Consistent with protein count |
Chain B
2117–2337(221 aa)
Fragment:Knl1 C-terminal domain, UNP residues 2117-2337
|
Non-standard monomer:Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 7;293 K;16-20 % (v/v) PEG 3350, 220 mM sodium citrate and 100 mM MES (pH 6 or 6.5) and 100 mM Hepes (pH 7), VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.80 Å R-free 0.265 |
| 4NFA Structure of the C-terminal doamin of Knl1 Deposited 2013-10-31 | Assembly 1 Protein monomer Monomer;Protein × 1 PDB declaration: monomeric(1) Consistent with protein count |
Chain A
2117–2323(207 aa)
Fragment:Knl1 C-terminal domain, UNP residues 2131-2337
|
Not recorded | CL CHLORIDE ION × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP;pH 9;293 K;120-300 KSCN, 100 mM BisTris Propane (pH 8.6-9.2), 17 % (v/v) PEG 3350 and 1 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.50 Å R-free 0.242 |
| 8PPR Structure of the human outer kinetochore KMN network complex Deposited 2023-07-08 | Assembly 1 Protein heterocomplex Heteromer;Protein × 8 PDB declaration: octameric(8) Consistent with protein count |
Chain K
1–2342(2342 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å |
| 8Q5H Human KMN network (outer kinetochore) Deposited 2023-08-09 | Assembly 1 Protein heterocomplex Heteromer;Protein × 7 PDB declaration: heptameric(7) Consistent with protein count |
Chain K
2021–2342(322 aa)
|
Not recorded | No recorded non-water small molecule | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å |