Current Protein Identity:Q8WVM0
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 6AAX Crystal structure of TFB1M and h45 with SAM in homo sapiens Deposited 2018-07-19 | Assembly 1 Protein–RNA Monomer;Protein × 1 PDB declaration: dimeric(2) Consistent with all polymers |
Chain A
28–346(319 aa)
|
Not recorded | SAM S-ADENOSYLMETHIONINE × 1 PEG DI(HYDROXYETHYL)ETHER × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7;293 K;0.2M potassium chloride, 0.025M magnesium sulfate hydrate, 0.05M HEPES sodium, pH 7.0, 20% v/v polyethylene glycol 200
|
Resolution 2.99 Å R-free 0.271 |
| 6AAX Crystal structure of TFB1M and h45 with SAM in homo sapiens Deposited 2018-07-19 | Assembly 2 Protein–RNA Monomer;Protein × 1 PDB declaration: dimeric(2) Consistent with all polymers |
Chain C
28–346(319 aa)
|
Not recorded | SAM S-ADENOSYLMETHIONINE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 7;293 K;0.2M potassium chloride, 0.025M magnesium sulfate hydrate, 0.05M HEPES sodium, pH 7.0, 20% v/v polyethylene glycol 200
|
Resolution 2.99 Å R-free 0.271 |
| 6AJK Crystal structure of TFB1M and h45 in homo sapiens Deposited 2018-08-28 | Assembly 1 Protein–RNA Monomer;Protein × 1 PDB declaration: dimeric(2) Consistent with all polymers |
Chain A
28–346(319 aa)
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP;pH 5;293 K;0.1M sodium citrate, pH 5.0, 20% PEG 8000
|
Resolution 3.00 Å R-free 0.241 |
| 8CSP Human mitochondrial small subunit assembly intermediate (State A) Deposited 2022-05-13 | Assembly 1 Protein–RNA Heteromer;Protein × 32 PDB declaration: 33-meric(33) Consistent with all polymers |
Chain 5
1–346(346 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 4 MG MAGNESIUM ION × 28 FES FE2/S2 (INORGANIC) CLUSTER × 2 ZN ZINC ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.66 Å |
| 8CSQ Human mitochondrial small subunit assembly intermediate (State B) Deposited 2022-05-13 | Assembly 1 Protein–RNA Heteromer;Protein × 32 PDB declaration: 33-meric(33) Consistent with all polymers |
Chain 5
1–346(346 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 4 MG MAGNESIUM ION × 27 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.54 Å |
| 8CSR Human mitochondrial small subunit assembly intermediate (State C) Deposited 2022-05-13 | Assembly 1 Protein–RNA Heteromer;Protein × 33 PDB declaration: 34-meric(34) Consistent with all polymers |
Chain 5
1–346(346 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 5 MG MAGNESIUM ION × 31 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.54 Å |
| 8CSU Human mitochondrial small subunit assembly intermediate (State C*) Deposited 2022-05-13 | Assembly 1 Protein–RNA Heteromer;Protein × 32 PDB declaration: 33-meric(33) Consistent with all polymers |
Chain 5
1–346(346 aa)
|
Not recorded | SF4 IRON/SULFUR CLUSTER × 1 SAM S-ADENOSYLMETHIONINE × 1 K POTASSIUM ION × 5 MG MAGNESIUM ION × 26 NAD NICOTINAMIDE-ADENINE-DINUCLEOTIDE × 1 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.03 Å |
| 9G5B Assembly intermediate of human mitochondrial ribosome small subunit (State A) Deposited 2024-07-16 | Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers |
Chain c
1–346(346 aa)
|
Not recorded | MG MAGNESIUM ION × 12 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.4;20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å |
| 9H55 Assembly intermediate of human mitochondrial ribosome small subunit in complex with NOA1 and TFB1M (state N3) Deposited 2024-10-22 | Assembly 1 Protein–RNA Heteromer;Protein × 31 PDB declaration: 32-meric(32) Consistent with all polymers |
Chain 5
1–346(346 aa)
|
Not recorded | MG MAGNESIUM ION × 41 K POTASSIUM ION × 10 ZN ZINC ION × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ATP ADENOSINE-5'-TRIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 2 | ELECTRON MICROSCOPY |
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å |