当前蛋白身份：Q91MK1
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差异标签只比较当前检索结果；所有PDB和assembly原始记录仍分别保留。
相关结构差异明细
一行代表一个 PDB 条目中的一个 biological assembly；同一蛋白的多个单体会分别列出。
| PDB 条目 | Assembly / 聚集状态 | 构建体 | 突变与修饰 | 配体、离子与非聚合物 | 实验方法 | 实验环境 | 结构质量 |
|---|---|---|---|---|---|---|---|
| 4KYC Structure of the C-terminal domain of the Menangle virus phosphoprotein, fused to MBP. 提交 2013-05-28 | Assembly 1 信息不足 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
339–388（50 aa）
片段：unprot P0AEX9 residues 27-392, unprot Q91MK1 residues 339-388
|
突变：E172A, N173A, E359A, K362A, D363A, C352S | EDO 1,2-ETHANEDIOL × 1 BO3 BORIC ACID × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 9.1；291.15 K；24 %(w/v) PEG8000, 0.2 M Boric acid/KOH, pH 9.1, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K
|
分辨率 1.95 Å R-free 0.213 |
| 7KD4 Structure of the C-terminal domain of the Menangle virus phosphoprotein (residues 329 -388), fused to MBP. Space group P21. 提交 2020-10-08 | Assembly 1 信息不足 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
329–388（60 aa）
|
突变：C352S | SO4 SULFATE ION × 9 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.5；291.15 K；1.65 M Ammonium sulphate, 0.2M Malic acid/KOH pH 5.5, Crystals were transferred into the following cryo-protective solution before vitrification: 1.65 M Ammonium sulphate, 0.2M Malic acid/KOH pH 5.5, 5mM Maltose, 1 M Lithium sulfate
|
分辨率 1.31 Å R-free 0.196 |
| 7KD4 Structure of the C-terminal domain of the Menangle virus phosphoprotein (residues 329 -388), fused to MBP. Space group P21. 提交 2020-10-08 | Assembly 2 信息不足 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 B
329–388（60 aa）
|
突变：C352S | SO4 SULFATE ION × 7 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 5.5；291.15 K；1.65 M Ammonium sulphate, 0.2M Malic acid/KOH pH 5.5, Crystals were transferred into the following cryo-protective solution before vitrification: 1.65 M Ammonium sulphate, 0.2M Malic acid/KOH pH 5.5, 5mM Maltose, 1 M Lithium sulfate
|
分辨率 1.31 Å R-free 0.196 |
| 7KD5 Structure of the C-terminal domain of the Menangle virus phosphoprotein (residues 329 -388), fused to MBP. Space group P212121 提交 2020-10-08 | Assembly 1 信息不足 单体；蛋白 × 1 PDB 声明：monomeric（1） 与蛋白数一致 |
链 A
329–388（60 aa）
|
突变：C352S | EDO 1,2-ETHANEDIOL × 4 PIN PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) × 3 PRO PROLINE × 1 | X-RAY DIFFRACTION |
X-ray结晶条件
VAPOR DIFFUSION, SITTING DROP；pH 6.7；291.15 K；20%(w/v) PEG 5000 mono-methyl ether, 0.2 M Pipes/KOH pH 6.7, 0.1M Proline, Crystals were transferred into the following cryo-protective solution before vitrification: 20%(w/v) PEG 5000 mono-methyl ether, 0.2 M Pipes/KOH pH 6.7, 0.1M Proline, 5mM Maltose, 20%(v/v) Ethylene Glycol
|
分辨率 1.55 Å R-free 0.189 |