Current Protein Identity：Q96EP0
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Difference tags compare only the current result set; every original PDB and assembly record remains separate.
Related-Structure Differences
Each row represents one biological assembly in one PDB entry; multiple monomers of the same protein are listed separately.
| PDB Entry | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Experimental Method | Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2CT7 Solution Structure of the IBR domain of the RING finger protein 31 protein Deposited 2005-05-23 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
779–851（73 aa）
Fragment：IBR domain
|
Not recorded | ZN ZINC ION × 2 | SOLUTION NMR |
NMR measurement conditions
pH 7；298 K；Ionic strength (raw mmCIF value) 220；Pressure ambient
NMR sample composition
1.55mM IBR domain U-13C,15N; 20mM d-Tris-HCl; 200mM NaCl; 1mM d-DTT; 0.02% NaN3; 0.01mM ZnCl2; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 4DBG Crystal structure of HOIL-1L-UBL complexed with a HOIP-UBA derivative Deposited 2012-01-15 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
480–636（157 aa）
Fragment：Ub-associated domain, residues 480-636
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；15%(w/v) PEG6000, 0.1M KCl, 0.1M HEPES-Na buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.71 Å R-free 0.253 |
| 4JUY Crystal structure of the PUB domain of E3 ubiquitin ligase RNF31 Deposited 2013-03-25 | Assembly 1 Protein homooligomer Homooligomer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
1–180（180 aa）
Chain B
1–180（180 aa）
|
Not recorded | UNX UNKNOWN LIGAND × 40 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；0.8M K/Na tartrate tetrahydrate, 0.5% PEG MME 5000, 0.1 M BisTris, pH 8.5, vapor diffusion, hanging drop, temperature 291K
|
Resolution 2.40 Å R-free 0.258 |
| 4LJO Structure of an active ligase (HOIP)/ubiquitin transfer complex Deposited 2013-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
853–1072（220 aa）
Fragment：E3 ligase HOIP catalytic core (unp residues 853-1072)
|
Not recorded | ZN ZINC ION × 5 IMD IMIDAZOLE × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 6.5；293 K；0.1 M carboxylic acids, 0.1 M imidazole, MES, 30 % P550 MME_P20K, pH 6.5, vapour diffusion, temperature 293K, VAPOR DIFFUSION
|
Resolution 1.56 Å R-free 0.212 |
| 4LJP Structure of an active ligase (HOIP-H889A)/ubiquitin transfer complex Deposited 2013-07-05 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
853–1072（220 aa）
Fragment：E3 ligase HOIP catalytic core (unp residues 853-1072)
|
Mutation：H889A | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.5；293 K；0.1 M amino acids, 0.1 M imidazole, MES, 30 % P550 MME_P20K , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.15 Å R-free 0.216 |
| 4LJQ Crystal structure of the catalytic core of E3 ligase HOIP Deposited 2013-07-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
853–1072（220 aa）
Fragment：Catalytic domain (unp residues 853-1072)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；20% PEG 12000, 800 mM LiCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.45 Å R-free 0.243 |
| 4LJQ Crystal structure of the catalytic core of E3 ligase HOIP Deposited 2013-07-05 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
853–1072（220 aa）
Fragment：Catalytic domain (unp residues 853-1072)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 5 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；20% PEG 12000, 800 mM LiCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.45 Å R-free 0.243 |
| 4LJQ Crystal structure of the catalytic core of E3 ligase HOIP Deposited 2013-07-05 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
853–1072（220 aa）
Fragment：Catalytic domain (unp residues 853-1072)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；20% PEG 12000, 800 mM LiCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.45 Å R-free 0.243 |
| 4LJQ Crystal structure of the catalytic core of E3 ligase HOIP Deposited 2013-07-05 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
853–1072（220 aa）
Fragment：Catalytic domain (unp residues 853-1072)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；20% PEG 12000, 800 mM LiCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.45 Å R-free 0.243 |
| 4LJQ Crystal structure of the catalytic core of E3 ligase HOIP Deposited 2013-07-05 | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
853–1072（220 aa）
Fragment：Catalytic domain (unp residues 853-1072)
Chain C
853–1072（220 aa）
Fragment：Catalytic domain (unp residues 853-1072)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 9 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；20% PEG 12000, 800 mM LiCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.45 Å R-free 0.243 |
| 4LJQ Crystal structure of the catalytic core of E3 ligase HOIP Deposited 2013-07-05 | Assembly 6 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
853–1072（220 aa）
Fragment：Catalytic domain (unp residues 853-1072)
Chain D
853–1072（220 aa）
Fragment：Catalytic domain (unp residues 853-1072)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) Non-standard monomer：Yes (specific site not provided by mmCIF) | ZN ZINC ION × 9 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；20% PEG 12000, 800 mM LiCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
|
Resolution 2.45 Å R-free 0.243 |
| 4OWF Crystal structure of the NEMO CoZi in complex with HOIP NZF1 domain Deposited 2014-01-31 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain G
199–228（30 aa）
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；Co-crystals were obtained after 6 days in 20% (w/v) PEG-3350 and 0.2 M DL-malic acid
|
Resolution 2.00 Å R-free 0.313 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 10 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain J
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 11 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain K
1–184（184 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 12 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain L
1–184（184 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 13 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain M
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain D
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain F
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain G
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain H
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYJ Structure of the apo HOIP PUB domain Deposited 2014-02-12 | Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain I
1–184（184 aa）
|
Not recorded | SO4 SULFATE ION × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；295 K；1.3 M ammonium sulphate, 200 mM KI, 100 mM Tris, pH 8.5
|
Resolution 3.00 Å R-free 0.255 |
| 4OYK Structure of HOIP PUB domain bound to OTULIN PIM Deposited 2014-02-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
3–179（177 aa）
|
Not recorded | CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.4；295 K；32% PEG 6000, 1M LiCl, 100 mM Tris pH 8.4
|
Resolution 2.00 Å R-free 0.237 |
| 4OYK Structure of HOIP PUB domain bound to OTULIN PIM Deposited 2014-02-12 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
3–179（177 aa）
|
Not recorded | CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.4；295 K；32% PEG 6000, 1M LiCl, 100 mM Tris pH 8.4
|
Resolution 2.00 Å R-free 0.237 |
| 4P09 Crystal structure of HOIP PUB domain Deposited 2014-02-20 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
1–179（179 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8.5；293 K；0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 8.5
|
Resolution 1.70 Å R-free 0.230 |
| 4P0A Crystal structure of HOIP PUB domain in complex with p97 PIM Deposited 2014-02-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–179（179 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 6.5
|
Resolution 2.30 Å R-free 0.249 |
| 4P0A Crystal structure of HOIP PUB domain in complex with p97 PIM Deposited 2014-02-20 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–179（179 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 6.5
|
Resolution 2.30 Å R-free 0.249 |
| 4P0B Crystal structure of HOIP PUB domain in complex with OTULIN PIM Deposited 2014-02-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–179（179 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 7.5
|
Resolution 2.70 Å R-free 0.270 |
| 4P0B Crystal structure of HOIP PUB domain in complex with OTULIN PIM Deposited 2014-02-20 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
1–179（179 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；0,1M Tris, 20% 2-methyl-2,4-pentanediol, 15% Polyethyleneglycol 3350, pH 7.5
|
Resolution 2.70 Å R-free 0.270 |
| 5EDV Structure of the HOIP-RBR/UbcH5B~ubiquitin transfer complex Deposited 2015-10-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain A
696–1072（377 aa）
Fragment：UNP residues 696-1072
|
Not recorded | ZN ZINC ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；285 K；0.06M Tris, 0.04M Bicine, 0.02 M 1,6-Hexanediol, 0.02 M 1-Butanol, 0.02 M 1,2-
Propanediol (racemic), 0.02 M 2-Propanol, 0.02 M 1,4-Butanediol, 0.02 M 1,3-Propanediol, 20% PEG550MME, 10% PEG20K, 8% glycerol
|
Resolution 3.48 Å R-free 0.303 |
| 5EDV Structure of the HOIP-RBR/UbcH5B~ubiquitin transfer complex Deposited 2015-10-22 | Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric（8） Consistent with protein count |
Chain A
696–1072（377 aa）
Fragment：UNP residues 696-1072
Chain B
696–1072（377 aa）
Fragment：UNP residues 696-1072
|
Not recorded | ZN ZINC ION × 16 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；285 K；0.06M Tris, 0.04M Bicine, 0.02 M 1,6-Hexanediol, 0.02 M 1-Butanol, 0.02 M 1,2-
Propanediol (racemic), 0.02 M 2-Propanol, 0.02 M 1,4-Butanediol, 0.02 M 1,3-Propanediol, 20% PEG550MME, 10% PEG20K, 8% glycerol
|
Resolution 3.48 Å R-free 0.303 |
| 5EDV Structure of the HOIP-RBR/UbcH5B~ubiquitin transfer complex Deposited 2015-10-22 | Assembly 3 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric（4） Consistent with protein count |
Chain B
696–1072（377 aa）
Fragment：UNP residues 696-1072
|
Not recorded | ZN ZINC ION × 8 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；285 K；0.06M Tris, 0.04M Bicine, 0.02 M 1,6-Hexanediol, 0.02 M 1-Butanol, 0.02 M 1,2-
Propanediol (racemic), 0.02 M 2-Propanol, 0.02 M 1,4-Butanediol, 0.02 M 1,3-Propanediol, 20% PEG550MME, 10% PEG20K, 8% glycerol
|
Resolution 3.48 Å R-free 0.303 |
| 5LJN Structure of the HOIP PUB domain bound to SPATA2 PIM peptide Deposited 2016-07-18 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
5–176（172 aa）
|
Not recorded | SO4 SULFATE ION × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；1.7-1.9 M (NH4)2SO4, 50 mM sodium cacodylate pH 6.4-7.0, 15 mM MgCl2
Protein:precipitant ratio; 1:2
200 nl drops
|
Resolution 2.70 Å R-free 0.281 |
| 5LJN Structure of the HOIP PUB domain bound to SPATA2 PIM peptide Deposited 2016-07-18 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
5–176（172 aa）
|
Not recorded | SO4 SULFATE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；1.7-1.9 M (NH4)2SO4, 50 mM sodium cacodylate pH 6.4-7.0, 15 mM MgCl2
Protein:precipitant ratio; 1:2
200 nl drops
|
Resolution 2.70 Å R-free 0.281 |
| 5X0W Molecular mechanism for the binding between Sharpin and HOIP Deposited 2017-01-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
480–639（160 aa）
Fragment：UNP residues 480-639
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.3；289 K；0.1 M HEPES (pH 7.3), 7% (w/v) PEG8000, 8% (v/v) ethylene glycerol
|
Resolution 3.00 Å R-free 0.302 |
| 5X0W Molecular mechanism for the binding between Sharpin and HOIP Deposited 2017-01-23 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
480–639（160 aa）
Fragment：UNP residues 480-639
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.3；289 K；0.1 M HEPES (pH 7.3), 7% (w/v) PEG8000, 8% (v/v) ethylene glycerol
|
Resolution 3.00 Å R-free 0.302 |
| 5X0W Molecular mechanism for the binding between Sharpin and HOIP Deposited 2017-01-23 | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain E
480–639（160 aa）
Fragment：UNP residues 480-639
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.3；289 K；0.1 M HEPES (pH 7.3), 7% (w/v) PEG8000, 8% (v/v) ethylene glycerol
|
Resolution 3.00 Å R-free 0.302 |
| 5X0W Molecular mechanism for the binding between Sharpin and HOIP Deposited 2017-01-23 | Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain G
480–639（160 aa）
Fragment：UNP residues 480-639
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.3；289 K；0.1 M HEPES (pH 7.3), 7% (w/v) PEG8000, 8% (v/v) ethylene glycerol
|
Resolution 3.00 Å R-free 0.302 |
| 6GZY HOIP-fragment5 complex Deposited 2018-07-05 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
853–1072（220 aa）
|
Not recorded | FHH methyl 4-[(2-oxidanylidene-1,5,6,7-tetrahydrocyclopenta[b]pyridin-3-yl)carbonylamino]butanoate × 1 ZN ZINC ION × 4 SO4 SULFATE ION × 4 EDO 1,2-ETHANEDIOL × 2 PGE TRIETHYLENE GLYCOL × 1 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；0.1 M Tris HCl pH 8.5, 28% w/v PEG 4000, 0.2 M Li2SO4
|
Resolution 2.15 Å R-free 0.246 |
| 6GZY HOIP-fragment5 complex Deposited 2018-07-05 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
853–1072（220 aa）
|
Not recorded | FHH methyl 4-[(2-oxidanylidene-1,5,6,7-tetrahydrocyclopenta[b]pyridin-3-yl)carbonylamino]butanoate × 1 ZN ZINC ION × 4 SO4 SULFATE ION × 3 EDO 1,2-ETHANEDIOL × 2 NA SODIUM ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；291 K；0.1 M Tris HCl pH 8.5, 28% w/v PEG 4000, 0.2 M Li2SO4
|
Resolution 2.15 Å R-free 0.246 |
| 6KC5 HOIP-HOIPIN1 complex Deposited 2019-06-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain B
853–1072（220 aa）
|
Not recorded | ZN ZINC ION × 4 D5U 2-[3-(2-methoxyphenyl)-3-oxidanylidene-propyl]benzoic acid × 1 GOL GLYCEROL × 4 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；293 K；100 mM HEPES-Na (pH 7.5), 200 mM NaCl, 25% PEG 3,350
|
Resolution 1.54 Å R-free 0.217 |
| 6KC6 HOIP-HOIPIN8 complex Deposited 2019-06-27 | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain A
853–1072（220 aa）
|
Not recorded | ZN ZINC ION × 4 D60 2-[3-[2,6-bis(fluoranyl)-4-(1~{H}-pyrazol-4-yl)phenyl]-3-oxidanylidene-propyl]-4-(1-methylpyrazol-4-yl)benzoic acid × 1 CL CHLORIDE ION × 3 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；200 mM KCl, 20% PEG3,350
|
Resolution 2.12 Å R-free 0.267 |
| 6KC6 HOIP-HOIPIN8 complex Deposited 2019-06-27 | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain C
853–1072（220 aa）
|
Not recorded | ZN ZINC ION × 4 D60 2-[3-[2,6-bis(fluoranyl)-4-(1~{H}-pyrazol-4-yl)phenyl]-3-oxidanylidene-propyl]-4-(1-methylpyrazol-4-yl)benzoic acid × 1 CL CHLORIDE ION × 1 GOL GLYCEROL × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；200 mM KCl, 20% PEG3,350
|
Resolution 2.12 Å R-free 0.267 |
| 6KC6 HOIP-HOIPIN8 complex Deposited 2019-06-27 | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain E
853–1072（220 aa）
|
Not recorded | ZN ZINC ION × 4 D60 2-[3-[2,6-bis(fluoranyl)-4-(1~{H}-pyrazol-4-yl)phenyl]-3-oxidanylidene-propyl]-4-(1-methylpyrazol-4-yl)benzoic acid × 1 CL CHLORIDE ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；200 mM KCl, 20% PEG3,350
|
Resolution 2.12 Å R-free 0.267 |
| 6KC6 HOIP-HOIPIN8 complex Deposited 2019-06-27 | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain G
853–1072（220 aa）
|
Not recorded | ZN ZINC ION × 4 D60 2-[3-[2,6-bis(fluoranyl)-4-(1~{H}-pyrazol-4-yl)phenyl]-3-oxidanylidene-propyl]-4-(1-methylpyrazol-4-yl)benzoic acid × 1 CL CHLORIDE ION × 1 GOL GLYCEROL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；200 mM KCl, 20% PEG3,350
|
Resolution 2.12 Å R-free 0.267 |
| 6KC6 HOIP-HOIPIN8 complex Deposited 2019-06-27 | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain I
853–1072（220 aa）
|
Not recorded | ZN ZINC ION × 4 D60 2-[3-[2,6-bis(fluoranyl)-4-(1~{H}-pyrazol-4-yl)phenyl]-3-oxidanylidene-propyl]-4-(1-methylpyrazol-4-yl)benzoic acid × 1 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；200 mM KCl, 20% PEG3,350
|
Resolution 2.12 Å R-free 0.267 |
| 6KC6 HOIP-HOIPIN8 complex Deposited 2019-06-27 | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric（1） Consistent with protein count |
Chain K
853–1072（220 aa）
|
Not recorded | ZN ZINC ION × 4 D60 2-[3-[2,6-bis(fluoranyl)-4-(1~{H}-pyrazol-4-yl)phenyl]-3-oxidanylidene-propyl]-4-(1-methylpyrazol-4-yl)benzoic acid × 1 CL CHLORIDE ION × 1 GOL GLYCEROL × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；200 mM KCl, 20% PEG3,350
|
Resolution 2.12 Å R-free 0.267 |
| 6SC5 dAb3/HOIP-RBR-Ligand2 Deposited 2019-07-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
697–1072（376 aa）
|
Not recorded | L6B methyl 4-[(2-oxidanylidene-5,6,7,8-tetrahydro-1~{H}-quinolin-3-yl)carbonylamino]but-3-enoate × 1 ZN ZINC ION × 8 SO4 SULFATE ION × 4 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Ammonium sulfate, sodium chloride, HEPES
|
Resolution 2.10 Å R-free 0.252 |
| 6SC6 dAb3/HOIP-RBR apo structure Deposited 2019-07-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
697–1072（376 aa）
|
Not recorded | ZN ZINC ION × 8 SO4 SULFATE ION × 5 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Ammonium sulfate, sodium chloride, HEPES
|
Resolution 2.25 Å R-free 0.246 |
| 6SC7 dAb3/HOIP-RBR-Ligand3 Deposited 2019-07-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
697–1072（376 aa）
|
Not recorded | L6H [2-[3-(cyclooct-4-en-1-yloxycarbonylamino)propylamino]-2-oxidanylidene-ethyl] (~{E})-4-[(2-oxidanylidene-5,6,7,8-tetrahydro-1~{H}-quinolin-3-yl)carbonylamino]but-2-enoate × 1 ZN ZINC ION × 8 SO4 SULFATE ION × 4 CL CHLORIDE ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Ammonium sulfate, sodium chloride, HEPES
|
Resolution 2.56 Å R-free 0.267 |
| 6SC8 dAb3/HOIP-RBR-Ligand4 Deposited 2019-07-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
697–1072（376 aa）
|
Not recorded | L6E [2-(methylamino)-2-oxidanylidene-ethyl] (~{E})-4-[(2-oxidanylidene-5,6,7,8-tetrahydro-1~{H}-quinolin-3-yl)carbonylamino]but-2-enoate × 1 ZN ZINC ION × 8 SO4 SULFATE ION × 3 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Ammonium sulfate, sodium chloride, HEPES
|
Resolution 2.11 Å R-free 0.261 |
| 6SC9 dAb3/HOIP-RBR-HOIPIN-8 Deposited 2019-07-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
697–1072（376 aa）
|
Not recorded | L68 2-[3-[2,6-bis(fluoranyl)-4-(1~{H}-pyrazol-4-yl)phenyl]-3-oxidanylidene-prop-1-enyl]-4-(1-methylpyrazol-4-yl)benzoic acid × 1 ZN ZINC ION × 8 SO4 SULFATE ION × 2 CL CHLORIDE ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；Ammonium sulfate, sodium chloride, HEPES
|
Resolution 2.47 Å R-free 0.252 |
| 7TV4 Crystal structure of NEMO CoZi in complex with HOIP NZF1 and linear diubiquitin Deposited 2022-02-03 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain K
350–379（30 aa）
Fragment：Residues 350-379
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；0.1 M Tris-HCl, pH 8.5, 22% v/v PEG Smear Broad
|
Resolution 4.20 Å R-free 0.286 |
| 7UY2 Structure of RNF31 in complex with FP06649, a Helicon Polypeptide Deposited 2022-05-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–179（179 aa）
|
Not recorded | NH2 AMINO GROUP × 1 WHL N,N'-(1,4-phenylene)diacetamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；0.06 M Divalents (0.3M Magnesium chloride hexahydrate; 0.3M Calcium chloride dihydrate), 0.1 M Buffer System 1 (Imidazole; MES monohydrate (acid) pH 6.5), 37.5% Precipitant Mix 4 (25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350)
|
Resolution 2.51 Å R-free 0.270 |
| 7UY2 Structure of RNF31 in complex with FP06649, a Helicon Polypeptide Deposited 2022-05-06 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–179（179 aa）
|
Not recorded | NH2 AMINO GROUP × 1 WHL N,N'-(1,4-phenylene)diacetamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；0.06 M Divalents (0.3M Magnesium chloride hexahydrate; 0.3M Calcium chloride dihydrate), 0.1 M Buffer System 1 (Imidazole; MES monohydrate (acid) pH 6.5), 37.5% Precipitant Mix 4 (25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350)
|
Resolution 2.51 Å R-free 0.270 |
| 7UYJ Structure of RNF31 in complex with FP06652, a Helicon Polypeptide Deposited 2022-05-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
1–179（179 aa）
|
Not recorded | NH2 AMINO GROUP × 1 WHL N,N'-(1,4-phenylene)diacetamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；0.1 M Amino Acids (0.2M DL-Glutamic acid monohydrate; 0.2M DL-Alanine; 0.2M Glycine; 0.2M DL-Lysine monohydrochloride; 0.2M DL-Serine),0.1 M Buffer System 1 (Imidazole; MES monohydrate (acid) pH 6.5), 30.0% Precipitant Mix 1 (40% v/v PEG 500* MME; 20 % w/v PEG 20000)
|
Resolution 2.32 Å R-free 0.274 |
| 7UYJ Structure of RNF31 in complex with FP06652, a Helicon Polypeptide Deposited 2022-05-06 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
1–179（179 aa）
|
Not recorded | NH2 AMINO GROUP × 1 WHL N,N'-(1,4-phenylene)diacetamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；0.1 M Amino Acids (0.2M DL-Glutamic acid monohydrate; 0.2M DL-Alanine; 0.2M Glycine; 0.2M DL-Lysine monohydrochloride; 0.2M DL-Serine),0.1 M Buffer System 1 (Imidazole; MES monohydrate (acid) pH 6.5), 30.0% Precipitant Mix 1 (40% v/v PEG 500* MME; 20 % w/v PEG 20000)
|
Resolution 2.32 Å R-free 0.274 |
| 7UYK Structure of RNF31 in complex with FP06655, a Helicon Polypeptide Deposited 2022-05-06 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain A
480–639（160 aa）
|
Not recorded | NH2 AMINO GROUP × 1 WHL N,N'-(1,4-phenylene)diacetamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；10% w/v glycerol, 20% w/v ethanol
|
Resolution 2.70 Å R-free 0.245 |
| 7UYK Structure of RNF31 in complex with FP06655, a Helicon Polypeptide Deposited 2022-05-06 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
480–639（160 aa）
|
Not recorded | NH2 AMINO GROUP × 1 WHL N,N'-(1,4-phenylene)diacetamide × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293.15 K；10% w/v glycerol, 20% w/v ethanol
|
Resolution 2.70 Å R-free 0.245 |
| 7V8F Crystal structure of UBE2L3 bound to HOIP RING1 domain. Deposited 2021-08-22 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain B
697–793（97 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；pH 8；289 K；2%(v/v) 1,4-dioxane, 0.1 M Tris-HCl (pH 8.0), 15%(w/v) PEG 3,350.
|
Resolution 1.66 Å R-free 0.198 |
| 7V8G Crystal structure of HOIP RING1 domain bound to IpaH1.4 LRR domain Deposited 2021-08-23 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
697–793（97 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；pH 8；289 K；2%(v/v) 1,4-dioxane, 0.1 M Tris-HCl (pH 8.0), 15%(v/v) PEG 3,350
|
Resolution 2.75 Å R-free 0.278 |
| 7V8G Crystal structure of HOIP RING1 domain bound to IpaH1.4 LRR domain Deposited 2021-08-23 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
697–793（97 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
EVAPORATION；pH 8；289 K；2%(v/v) 1,4-dioxane, 0.1 M Tris-HCl (pH 8.0), 15%(v/v) PEG 3,350
|
Resolution 2.75 Å R-free 0.278 |
| 8Z30 Crystal structure of HOIP PUB domain in complex with tolfenamic acid complex Deposited 2024-04-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
4–179（176 aa）
Chain B
4–179（176 aa）
Chain C
4–179（176 aa）
|
Not recorded | PEG DI(HYDROXYETHYL)ETHER × 3 TLF 2-[(3-chloro-2-methylphenyl)amino]benzoic acid × 3 PE4 2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL × 1 TOE 2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；20 mM Tris, 150 mM NaCl, pH 7.5, 10% w/v PEG1000, 10% w/v PEG8000
|
Resolution 2.30 Å R-free 0.242 |
| 8Z36 Crystal structure of HOIP PUB domain in complex with sertraline complex Deposited 2024-04-14 | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain A
4–179（176 aa）
Chain B
4–179（176 aa）
Chain C
4–179（176 aa）
|
Not recorded | SRE (1S,4S)-4-(3,4-dichlorophenyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine × 3 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；20 mM Na3PO4, 20 mM K3PO4, pH 7.5, 20% PEG 3350
|
Resolution 2.63 Å R-free 0.269 |
| 9AZJ Structure of ubiquitinated NEMO UBAN K285C-Ub G76C bound to HOIP NZF1 Deposited 2024-03-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 9 PDB declaration: nonameric（9） Consistent with protein count |
Chain E
351–379（29 aa）
Fragment：HOIP NZF domain, residues 351-379
Chain G
351–379（29 aa）
Fragment：HOIP NZF domain, residues 351-379
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.6；293 K；150nl protein + 50 nl mother liquor: 0.1 M Tris/Bicine pH 8.6, 24.2% PEG 500 MME, 8% PEG 20K, 0.03 M each of NaI, NaBr, and NaF. Cryoprotected in mother liquor containing 20% glycerol
|
Resolution 3.32 Å R-free 0.285 |
| 9B0B Structure of Optineurin bound to HOIP NZF1 domain Deposited 2024-03-11 | Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain G
350–379（30 aa）
|
Not recorded | PGE TRIETHYLENE GLYCOL × 5 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；293 K；3:1 (protein:mother liquor: 10% PEG 8K, 20% ethylene glycol, 0.1 M Tris/Bicine pH 8.5, 0.03 M MgCl2 and 0.03 M CaCl2. cryoprotected in mother liquor containing 10% glycerol
|
Resolution 1.70 Å R-free 0.222 |
| 9B0B Structure of Optineurin bound to HOIP NZF1 domain Deposited 2024-03-11 | Assembly 2 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric（3） Consistent with protein count |
Chain E
350–379（30 aa）
|
Not recorded | PGE TRIETHYLENE GLYCOL × 5 PG4 TETRAETHYLENE GLYCOL × 1 ZN ZINC ION × 1 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；293 K；3:1 (protein:mother liquor: 10% PEG 8K, 20% ethylene glycol, 0.1 M Tris/Bicine pH 8.5, 0.03 M MgCl2 and 0.03 M CaCl2. cryoprotected in mother liquor containing 10% glycerol
|
Resolution 1.70 Å R-free 0.222 |
| 9B0Z Structure of Optineurin bound to HOIP NZF1 domain and M1-linked diubiquitin, crystal form 2 Deposited 2024-03-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 7 PDB declaration: heptameric（7） Consistent with protein count |
Chain E
350–379（30 aa）
Chain F
350–379（30 aa）
|
Not recorded | ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8.5；293 K；20% PEG 2K MME, 0.2 M TAO and 0.1 M Tris pH 8.5. Cryoprotected in mother liquor containing 20% glycerol
|
Resolution 2.41 Å R-free 0.270 |
| 9B12 Structure of Optineurin bound to HOIP NZF1 domain and M1-linked diubiquitin, crystal form 1 Deposited 2024-03-12 | Assembly 1 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain G
350–379（30 aa）
Chain H
350–379（30 aa）
|
Not recorded | PG4 TETRAETHYLENE GLYCOL × 1 PGE TRIETHYLENE GLYCOL × 1 CL CHLORIDE ION × 1 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；293 K；3:1 with reservoir solution containing 50% PEG 200 and 0.1 M HEPES pH 7.5
|
Resolution 1.81 Å R-free 0.269 |
| 9B12 Structure of Optineurin bound to HOIP NZF1 domain and M1-linked diubiquitin, crystal form 1 Deposited 2024-03-12 | Assembly 2 Protein heterocomplex Heteromer；Protein × 5 PDB declaration: pentameric（5） Consistent with protein count |
Chain I
350–379（30 aa）
Chain J
350–379（30 aa）
|
Not recorded | PG4 TETRAETHYLENE GLYCOL × 1 CL CHLORIDE ION × 2 EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 3 ZN ZINC ION × 2 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；293 K；3:1 with reservoir solution containing 50% PEG 200 and 0.1 M HEPES pH 7.5
|
Resolution 1.81 Å R-free 0.269 |
| 9IIC Crystal structure of HOIP RING2-LDD in complex with STK4 KD domain Deposited 2024-06-20 | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain C
857–1071（215 aa）
Fragment：RING2-LDD
|
Not recorded | GOL GLYCEROL × 1 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289 K；0.2 M Ammonium citrate tribasic (pH 7.0), 20% w/v Polyethylene glycol 3350
|
Resolution 2.78 Å R-free 0.280 |
| 9IIC Crystal structure of HOIP RING2-LDD in complex with STK4 KD domain Deposited 2024-06-20 | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric（2） Consistent with protein count |
Chain D
857–1071（215 aa）
Fragment：RING2-LDD
|
Not recorded | GOL GLYCEROL × 1 ZN ZINC ION × 4 | X-RAY DIFFRACTION |
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；289 K；0.2 M Ammonium citrate tribasic (pH 7.0), 20% w/v Polyethylene glycol 3350
|
Resolution 2.78 Å R-free 0.280 |